• Restorative Dentistry and Endodontics
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• v.23 no.1
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• pp.328-338
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• 1998

Porphyromonas endodontalis, an anaerobic Gram negative cocobacillus which was known to be associated with the infected root canals and periapical lesions, is very difficult to culture and to detect by the traditional method in that it requires much time to induce the specific black pigmentation, and it is very sensitive to oxygen and the antibiotics added in the culture medium. In this study, the nucleotide sequences of the 'probe h' (0.73kb), one of the specific DNA probes top. endodontalis (ATCC 35406) which had been developed by our department, was determined and then a pair of primers for PCR amplification was fabricated to identify P. endodontalis. The plasmids containing 'probe h' were purified by $Wizard^{TM}$ Midipreps DNA Purification System (Promega Corp.), and the nucleotide sequences of the 'probe h' were determined by the dideoxy chain termination method using TaqTrack Sequencing System (Promega Corp.) and detected by fluorescent labelling method. The sense/antisense PCR primers were designed with computer software (Lasergene, DNASTAR Ind. PCR was done with a programmable GeneAmp PCR System 2400 (Perkin Elmer-Cetus Co.). Each sample containing the whole genomic DNA of P. endodontalis and other black-pigmented Bacteroides was itailly denatured at $94^{\circ}C$ for 5 min and then subjected to 30 cycles, each of them consisting of 60s at $94^{\circ}C$, 60s at $60^{\circ}C$, and 90s. at $72^{\circ}C$. The amplified DNA was resolved electrophoretically in a 1.0 % agarose gel in 1X TAE buffer, stained with EtBr, and photographed on a UV transilluminator. The results were as follows : 1. The nucleotide sequences of 'probe h' (743 base pairs) were obtained by dideoxy chain termination method, and from that results the specific primers to P. endodontalis (ATCC 35406), 'Primer H1/ Primer H2', were designed. 2. It has been found that 'Primer H1/H2' could detect P. endodontalis (ATCC 35406) using PCR. 3. The PCR system with this primers may be a powerful technique to amplify the specific sequences of 'probe h' of P. endodontalis (ATCC 35406) that produce distinct identification of it from other black-pigmented Bacteroides, and this could help us to determine the nature of periapical disease.

• Research in Plant Disease
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• v.24 no.4
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• pp.328-331
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• 2018

A severe disease with leaf spots and necrotic symptoms was observed in cucumber (Cucumis sativus L.) seedlings in April 2018 at a nursery in Kimjae, Korea (35o 47'09.8"N 127o 2'24.3"E). The infected plants initially showed spots on water-soaked cotyledons which, at later stages, enlarged and spread to the leaves, which the lesions becoming dry and chlorotic. The symptomatic samples were collected from cucumber and the isolates were cultured on LB agar. The representative bacterial strain selected for identification showed fluorescent on King's medium B, was potato rot-positive, levan and arginine dihydrolase-negative, oxidase-negative and tobacco hypersensitivity-positive in LOPAT group 2 as determined by LOPAT tests. A pathogenicity test was carried out on a 3-week-old cucumber. After 3 days of inoculation, leaf spots and necrotic symptoms appeared on the cucumber, similar to the originally infected plants. The infecting bacterial strain was identified as Pseudomonas viridiflava, by 16S rDNA sequence analysis. This is the first report of leaf spot diseases on cucumber caused by P. viridiflava.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2019.06a
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• pp.212-215
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• 2019

지난 4월4일 고성 산불로 사망자 2명과 부상자 1명, 가옥 500여 채, 삼림 1757ha가 불에 탔다. 강원 산불에 이어 영덕지진 등에서 늑장대응을 보여준 재난방송시스템에는 많은 국민들에게 실망감을 안겨주었다. 재난방송 주관방송사인 KBS는 물론, MBC, SBS의 경우도 재난방송시스템에 관련 된 측면에서 본다면 아직 이웃나라 인 일본에 비해서는 매우 열악한 형편이다. 그럼에도 불구하고 점점 대형화, 다발화 하는 재난발생에 대응하기 위해서는 국가적인 차원에서도 특단의 조치가 필요하다. 미국의 경우는 차세대 재난정보 전달체계인 IPAWS(Integrated Public Alert and Warning System)를 개발해 지상파뿐만 아니라, 케이블TV, SNS 등 다양한 매체를 통해 재난정보를 신속하게 전달하고 있다. 일본도 이와 유사한 재난경보전달시스템인 J-Alert를 개발해 2020년까지는 '재난 약자 제로(Zero)시대'를 목표로 구현하고 있다. 우리나라는 지난 아현동 KT 화재사건에서도 경험했듯이 통신이 먹통이 되는 통신블랙아웃도 경험했다. 따라서 대형재난발생 시는 신속한 재난경보전달시스템이 재난피해를 줄일 수 있는 가장 중요한 생명줄이 될 수 있다. 미국이나 일본의 경우는 재난방송전달시스템을 관련법령으로 제도화 하고 있다. 특히, 일본에는 재난에 관한 모법이라고 할 수 있는 (1)"재해대책기본법"이 있는데, 이는 재해로부터 국토, 국민의 생명과 재산을 보호하기 위한 기본법으로 규정되어 있다. 그 밖에도 (2)방송법 (3)대규모지진대책특별 조치법 (4)국민보호법 (5)소방조직법 (6)수해방지법 등으로 규정하고 있다. 과거 일본도 우리나라와 같이 대형 산불이 잦았으나 요즘은 소형 산불만 발생하는 추세다. 이는 NHK가 보유한 700여 대의 로봇카메라와 전 국토를 샅샅이 감시하는 CCTV 덕택이다. 또한, NHK 보도국의 '기상 재해센터'는 재난에 대비해 40여 명의 전문 인력이 24시간 대응체제를 갖추고 있다. 나아가 NHK는 전국 12개의 거점지역에 헬리콥터 15대를 배치하여 신속하게 취재하고 있다. 이 뿐만 아니라, 46개의 지역방송국을 7개의 거점방송국으로 분할하여, 거점방송국마다 40여명의 카메라맨을 상주시켜 언제든지 재난을 취재할 수 있도록 하고 있다. 세계 각국에서 사용하고 있는 방송 주파수는 공공재(公共材)다. 국제전기통신연합(ITU : International Telecommunication Union)으로부터 주파수를 할당받아 사용하고 있기 때문에 주파수에 관한 사용 권한은 각국의 국민 모두에게 있다. 그러나 효과적인 주파수 활용을 전제로 정부가 일정한 자격을 갖춘 방송사업자에게 일시적으로 주파수 사용권을 위임하고 있다. 따라서 일본 정부도 국가적인 위기나 대형 재난발생으로 국민들의 생명과 재산이 위협받고 있을 때에는 공공재인 주파수를 즉시 재난방송으로 사용할 수 있도록 <재해대책기본법 제6조>와 방송법 제108조에 규정하고 있다.

• Korean Journal of Child Education & Care
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• v.19 no.1
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• pp.45-57
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• 2019

Objective: The purpose of this study is to critically analyze the process of framing child abuse cases through the news coverage after the enforcement of mandatory CCTV installation policy which was initiated in 2015. Methods: The research uses the revised and supplemented frame analysis tools developed by Iyengar (1990) and Semetko and Valkenburg (2000) to investigate 25 broadcasting news reported by KBS, from September 2015 to December 2017. Results: First, according to the formal analysis, the reports on abuse cases prove to be either a mixed or episodic frame which covers the cases heavily based on a particular incident rather than holistically looks into the situation. Second, in content analysis, child abuse news is delivered mainly through "human interest frame." Conclusion/Implications: The study identifies three dominant discourses formed as a result of the child abuse CCTV news: 'CCTV is a all-around player' discourse, 'preschool teachers as potential criminals' discourse and 'preschool teachers as nonprofessionals' discourse. This study is significant in that it presents that mass media do not always say the truth, but frame social issues within an ideological delivery system.

• Journal of Embryo Transfer
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• v.33 no.4
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• pp.195-203
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• 2018

Interferon-tau (IFNT) is known as a major conceptus protein that signals the process of maternal recognition of pregnancy in ruminants. Also, multiple interferon genes exist in cattle, However, molecular mechanisms of these bovine IFNT (bIFNT) genes whose expressions are limited have not been characterized. We and others have observed that expression levels of bovine subtype IFNT genes in the tissues of ruminants; thus, bIFNT1 and other new type I (bIFNTc1/c2/c3) gene co-exist during the early stages of conceptus development and non-trophoblast cells. Its genes transcription could be regulated through CDX2 and ETS2 and JUN and/or cAMP-response element binding protein (CREB)-binding protein (CREBBP) expression, a transcription factor implicated in the control of cell differentiation in the trophectoderm. Bovine ear-derived fibroblast cells, were co-transfected with luciferase reporter constructs carrying upstream (positions -1000 to +51) regions of bIFNT1 and other new type I gene and various transcription factor expression plasmids. Compared to each - 1kb-bIFNT1/c1/c2/c3-Luc increased when this constructs were co-transfected with CDX2, ETS2, JUN and/or CREBBP. Also, Its genes was had very effect on activity by CDX2, either alone or with the other transcription factors, markedly increased luciferase activity. However, the degree of transcriptional activation of the bIFNTc1 gene was not similar to that bIFNT1/c2/c3 gene by expression plasmid. Furthermore, Sequence analyses also revealed that the expression levels of bIFNT1/c2/c3 gene mRNAs expression were highest on day 17, 20 and 22 trophoblast and, Madin-Darby bovine kidney (MDBK), Bovine ear-derived fibroblast (EF), and endometrium (Endo) non-trophoblast cells. But, bIFNTc1 mRNA had not same expression level, bIFNTc1 lowest levels than those of IFNT1/c2/c3 gene in both trophoblast and non-trophoblast cells. These results demonstrate that bovine subtype bIFNT genes display differential, in the trophoblast and non-trophoblast cells.

• The Journal of the Korea Contents Association
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• v.19 no.5
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• pp.644-657
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• 2019

This study examines the types of strategic alliances formed between terrestrial broadcasters and content start-ups, their resource inter-dependence and the dynamic evolution of their relationship through a case study of KBS Labs, a strategic alliance between KBS and content start-ups. Mergers, acquisitions and strategic alliances sought by large media companies or strategic alliances made only between disparate providers in broadcasting and telecommunications industries are now actively pursued by traditional media and start-ups. The alliances between traditional media and start-ups are beneficial to both parties: traditional media can quickly implement start-ups' innovative ideas and business models and start-ups can tap into not only vast amounts of content being created by or in the archives of traditional media but also their capital, office space and reputation. Therefore, the case study of KBS Labs will help draw out important implications for motivating strategic alliances between traditional media and start-ups. To this end, the resource-based view and the evolutionary theory of the firm are adopted to explore the characteristics and development process of the KBS Labs business.

• Journal of Industrial Convergence
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• v.20 no.1
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• pp.53-62
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• 2022

The election broadcast began utilizing virtual studios in the mid-1990s. In 1996, SBS's virtual studio was evaluated as an innovation in Korean broadcasting technology that introduced the world's first virtual studio. However, there have been cases where KBS and MBC named it a virtual studio and used it for election broadcasting. Various CG (Computer Graphics) case studies of election broadcasting have been conducted since the 2000s, but the initial research is inadequate. Therefore, this paper complements existing research by analyzing the cases of the mid-1990s when they actually started using virtual studios. Beginning with SMOCKEY (KBS) and MAGICII (MBC) in 1995, we presented the initial model of the virtual studio, and then with SBS Virtual Studio, each broadcasting company evolved into the names Dream Studio (KBS) and Space 21 Studio (MBC). As a result of the analysis, it was found that the election broadcast is a chart showing the data and the winning prediction, and that the election broadcast was the trigger to compose the story based on the introduction of the virtual studio. It is a form of historical research dealing with the value of the early virtual studios in this paper. It is meaningful to see the process of Remediation.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.202-202
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• 2022

Recently days, soybean production in paddy field is increasing, from 4,422 ha in 2016 to 10,658 ha in 2021 in Korea. It is easy for Phytophthora stem and root rot (PSR) occurring in paddy field condition, when it is poorly drained soils with a high clay content, and temporary flooding and ponding. Therefore PSR resistant soybean cultivar is required. The objective of this study is to identify QTL region and candidate genes relating to PSR resistance of the race in main soybean cultivation area in Korea. 210 soybean materials including cultivars and germplasm were used for inoculation and genome-wide association study (GWAS). Inoculation was conducted using stem-scar method with 2 replications in 2-year for the race 3053 from Kimje and 3617 from Andong. 210 materials were genotyped with Soya SNP 180K chip, and structure analysis and association mapping were conducted with QTLMAX V2. The results of inoculation showed that survival ratio ranged from 0% to 96.7% and mean 9.7% for 3053 and ranged from 0% to 100% and mean 7.6% for 3617. Structure analysis showed linkage disequillibrium (LD) was decayed below r²=0.5 at 335kb of SNP distance. Significant SNPs (LOD>7.0) were identified in Chr 1, 2, 3, 4, 5, 11, 14, 15 for 3053 and Chr 1, 2, 3, 7, 10, 14 for 3617. Especially, LD blocks (AX-90455181;15,056,628bp~AX-90475572;15,298,872bp) in Chr 2 for 3053 and 3067 were duplicated. 29 genes were identified on these genetic regions including Glyma.02gl47000 relating to ribosome recycling factor and defense response to fungus in Soybase.

• The Journal of the Acoustical Society of Korea
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• v.25 no.1
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• pp.21-29
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• 2006

In this Paper a split bandwidth wideband speech coder and its highband coding method are Proposed. The coder uses a split-band approach. where the wideband input speech signal is split into two equal frequency bands from 0-4kHz and 4-8kHz. The lowband and the highband are coded respectively by the 11.8kb/s G.729 Annex E and the proposed coding method. After the LPC analysis, the highband is divided by two modes according to the properties of signals. In stationary mode. the highband signals are compressed by the mixture excitation model; CELP algorithm and W (Matching Pursuit) algorithm. The others are coded by the only CELP algorithm. We compare the performance of the new wideband speech coder with that of G.722 48kbps SB-ADPCM and G.722.2 12.85kbps in a subjective method. The simulation results show that the Performance of the proposed wideband speech coder has better than that of 48kbps G.722 and no better than that of 12.85kbps G.722.2.

• Animal Bioscience
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• v.37 no.5
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• pp.795-806
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• 2024

Objective: Karan Fries (KF), a high-producing composite cattle was developed through crossing indicine Tharparkar cows with taurine bulls (Holstein Friesian, Brown Swiss, and Jersey), to increase the milk yield across India. This composite cattle population must maintain sufficient genetic diversity for long-term development and breed improvement in the coming years. The level of linkage disequilibrium (LD) measures the influence of population genetic forces on the genomic structure and provides insights into the evolutionary history of populations, while the decay of LD is important in understanding the limits of genome-wide association studies for a population. Effective population size (N_e) which is genomically based on LD accumulated over the course of previous generations, is a valuable tool for e valuation of the genetic diversity and level of inbreeding. The present study was undertaken to understand KF population dynamics through the estimation of N_e and LD for the long-term sustainability of these breeds. Methods: The present study included 96 KF samples genotyped using Illumina HDBovine array to estimate the effective population and examine the LD pattern. The genotype data were also obtained for other crossbreds (Santa Gertrudis, Brangus, and Beefmaster) and Holstein Friesian cattle for comparison purposes. Results: The average LD between single nucleotide polymorphisms (SNPs) was r² = 0.13 in the present study. LD decay (r² = 0.2) was observed at 40 kb inter-marker distance, indicating a panel with 62,765 SNPs was sufficient for genomic breeding value estimation in KF cattle. The pedigree-based N_e of KF was determined to be 78, while the N_e estimates obtained using LD-based methods were 52 (SNeP) and 219 (genetic optimization for N_e estimation), respectively. Conclusion: KF cattle have an N_e exceeding the FAO's minimum recommended level of 50, which was desirable. The study also revealed significant population dynamics of KF cattle and increased our understanding of devising suitable breeding strategies for long-term sustainable development.