Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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First Report of Pseudomonas viridiflava Causing Leaf Spot of Cucumber in Korea

Pseudomonas viridiflava에 의한 오이 점무늬병의 발생 보고

  • Seo, Yunhee (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science) ;
  • Park, Mi-Jeong (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science) ;
  • Back, Chang-Gi (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science) ;
  • Park, Jong-Han (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science)
  • 서윤희 (국립원예특작과학원원예특작환경과) ;
  • 박미정 (국립원예특작과학원원예특작환경과) ;
  • 백창기 (국립원예특작과학원원예특작환경과) ;
  • 박종한 (국립원예특작과학원원예특작환경과)
  • Received : 2018.09.10
  • Accepted : 2018.10.16
  • Published : 2018.12.31
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Abstract

A severe disease with leaf spots and necrotic symptoms was observed in cucumber (Cucumis sativus L.) seedlings in April 2018 at a nursery in Kimjae, Korea (35o 47'09.8"N 127o 2'24.3"E). The infected plants initially showed spots on water-soaked cotyledons which, at later stages, enlarged and spread to the leaves, which the lesions becoming dry and chlorotic. The symptomatic samples were collected from cucumber and the isolates were cultured on LB agar. The representative bacterial strain selected for identification showed fluorescent on King's medium B, was potato rot-positive, levan and arginine dihydrolase-negative, oxidase-negative and tobacco hypersensitivity-positive in LOPAT group 2 as determined by LOPAT tests. A pathogenicity test was carried out on a 3-week-old cucumber. After 3 days of inoculation, leaf spots and necrotic symptoms appeared on the cucumber, similar to the originally infected plants. The infecting bacterial strain was identified as Pseudomonas viridiflava, by 16S rDNA sequence analysis. This is the first report of leaf spot diseases on cucumber caused by P. viridiflava.

2018년 4월 전북 김제 오이 육묘장에서 오이의 자엽과 본엽에 반점이 형성되고 괴사되는 증상이 관찰되었다. 초기증상은 물방울이 잘 맺히는 자엽 부근에서 시작되었고, 병증상이 확대되면 본엽에 반점이 생기면서 백화 되어 마르는 증상이 관찰되었다. 세균병이 의심되는 식물체를 채집하여 LB agar에 계대하였다. 분리한 한 균주를 가지고 LOPAT test를 수행한 결과, KB agar에서 형광발현, 감자절편에서 무름 증상이 나타나며, Arginine dihydrolase 음성임을 확인하였다. oxidase를 형성하지 않고 담배과민성 실험에서 양성인 것을 확인하여 LOPAT 2그룹에 속하는 것을 확인하였다. 병원성 실험은 발아 후 3주된 오이에 접종하여 접종 3일 후 병징이 동일하게 발생된 것을 확인하였다. 16s rDNA 유전자 염기서열을 이용하여 염기서열분석과 계통수를 분석하여 분리된 균주가 P. viridiflava로 동정되었다. 이 병은 국내에서 처음으로 P. viridiflava에 의해 발생되는 오이점무늬병으로 보고하고자 한다.

Keywords

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Fig. 1. Symptoms of the leaf-spot disease on container-grown cucumber seedlings in a nursery (A). Water soaked spots with chlorotic lesions on the cotyledons (B). Small, irregular chlorotic spot on a true leaf (C).

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Fig. 2. Symptoms developing observed on cucumber after 3 days of incubation with P. viridiflava. Left: No symptoms were observed in control plants (A). Right: irregular spots, chlorotic and greyish necrotic spots were noted on leaves of the infected plants (B-D).

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Fig. 3. Phylogenetic analysis of the pathogen species using the neighbor-joining method on the basis of 16S rDNA sequences. The numbers beside branches represent the percentage of congruent clusters in 1,000 bootstrap trials. The bar indicates 2% sequence dissimilarity.

Table 1. Comparison of the biochemical characteristics of the isolates from infected cucumber and the other Pseudomonas spp.

SMBRCU_2018_v24n4_328_t0001.png 이미지

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