• Proceedings of the Korean Institute of Communication Sciences Conference
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• 2004.07a
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• pp.335-335
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• 2004

• Proceedings of the Zoological Society Korea Conference
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• 1999.10b
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• pp.219.1-219
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• 1999

No Abstract, See Full Text

• Korean Journal of Microbiology
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• v.30 no.6
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• pp.519-523
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• 1992

The glucoamylase of Candida tsuubaensis was purified to homogeneity form culture filtrate by means of ultrafiltration, Sephacryl S-200 gel filtration and Sp-Sephadex C-50 chromatography. The purified enzyme was a glycoprotein with a molecular mass of approximately 50 kDa, which was a monomeric protein. Km values were 5.8 mg/ml for soluble starch and 0.04 mM for maltose. Glucoamylase also released only glucose from both pullulan and isomaltose. The analysis of amino acid composition revealed that the enzyme contained a high content of acidic and polar amino acids. In addition, Western blotting analysis indicates that C. tsukubaensis glucoamylase is resistant to glucose repression.

• Journal of Microbiology and Biotechnology
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• v.11 no.1
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• pp.65-71
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• 2001

A gene from Paenibacillus sp. KCTC 8848P encoding ${\beta}-amylase$ was cloned and expressed in Escherichia coli. The Paenibacillus ${\beta}-amylase$ gene cosisted of a 2,409-bp open reading frame without a translational stop codon, encoding a protein of 803 amino acids. The presumed ribosime-binding site, GGAGG, was located 10 bp upstream from the TTG initiation codon. The deduced amino acid sequence of the ${\beta}-amylase$ gene had a 95% similarity to the ${\beta}-amylase$ of Bacillus firmus. The ${\beta}-amylase$ gene was introduced into wild-type strains of Saccharomyces cerevisiae using a linearized yeast integrating vector containing a geneticin resistance gene and its product was secreted into the culture medium.

• Journal of Microbiology and Biotechnology
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• v.12 no.2
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• pp.340-344
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• 2002

A gene, npl, encoding neopullulanase from Paenibacillus sp. KCTC 8848P was cloned and expressed in Escherichia coli. It consisted of an open reading frame of 1,530 bp for a protein that consisted of 510 amino acids with a molecular weight of 58,075 Da. The deduced amino acid sequence of the neopullulanase gene had $92\%$ identity with the neopullulanase of Bacillus polymyxa. The npl gene was also expressed in Saccharomyces cerevisiae secreting Schwanniomyces occidentalis glucoamylase (GAM1) under the control of the yeast actin gene (ACT1) promoter. Secretion of the neopullulanase was directed by the yeast mating pheromone ${\alpha}$ -factor ($MF{\alpha}1$) prepro region. Enzyme assays confirmed that co-expression of npl and GAM1 enhanced starch and pullulan degradation by S. cerevisiae.

• Fashion & Textile Research Journal
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• v.19 no.1
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• pp.40-48
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• 2017

In this study, the impact of the fashion design education on the brain wave and the psychological change of the senior females and its change was intended to analyze. 16 senior females were selected as a subject and the brain wave and the psychology were analyzed before and after the educational program of 4 hours a day for total 3 weeks. Out of the brain wave, the increase of alpha wave represents the mental stability and the comfortable state and its decrease represents the tension and stress. On the contrary, the increase of beta wave represents the tension and the excitation. In the brain wave analysis results, the alpha wave was increased and the beta wave was decreased after the design education program, through which it is deemed that the design education would help to increase the psychological stability and to decrease the tension and the stress in the senior females. In the results of analyzing the psychological test, while the test results for 4 senior females were shown as anxious out of 16 senior females, the BAI score was decreased to 'minor anxiety' after the education and the BAI score was decreased except 2 subjects, through it is deemed that the fashion design education would help to reduce the anxiety in the senior females and it seems that it is coincided with the beta wave analysis results.

• Journal of Oriental Neuropsychiatry
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• v.27 no.2
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• pp.67-76
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• 2016

Objectives The purpose of this study is to report that a patient with obsessive-compulsive disorder (OCD) significantly responds to Korean medical treatments and psychotherapy.Methods We treated the patient with Korean medical treatments (acupuncture and herbal medication) and Melonis Calyx Vomiting Therapy. We also used psychotherapy including Li-Gyeung-Byun-Qi therapy. The patient was diagnosed by DSM-IV diagnostic criteria for OCD. We used Obsessive-Compulsive Inventory-Revised-Korean (OCI-R-K), Visual Analogue Scale (VAS), Beck’s Depression Inventory (BDI), and Beck’s Anxiety Inventory (BAI) to evaluate the patient.Results OCI-R-K score was decreased from 39 points to 9 points. BDI score was decreased from 14 points to 6 points. BAI score was decreased from 25 points to 13 points.Conclusions This study suggests that Korean medical treatment is an effective way to treat patients who suffer from OCD.

• Journal of Microbiology and Biotechnology
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• v.9 no.5
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• pp.668-671
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• 1999

The gene encoding Schwanniomyces occidentalis $\alpha-amylase$(AMY) was introduced into Saccharomyces cerevisiae var. diastaticus which secreted only glucoamylase, by using a linearized yeast integrating vector to develop stable strains with a capability of secreting $\alpha-amylase$and glucoamylase simultaneously. A dominant selectable marker, the geneticin(G418) resistance gene (Gt^r$), was cloned into a vector to screen wild-type diploid transformants harboring the AMY gene. The amylolytic activities of transformants were about 3-7 times higher than those of the recipient strains. When grown in nonselective media, the transformants with the linearized integrating vector containing the AMY gene exhibited almost all of the mitotic stability after 100 generations.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.9
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• pp.387-393
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• 2016

The aim of this study is to analyze the effectiveness of QEEG-based neurofeedback clinical tests on patients diagnosed with anxiety disorder. Researchers tested six patients with anxiety disorder using 32-channel QEEG(Quantitative electroencephalograpy) and neurofeedback training equipment. The study measured anxiety levels of patients using QEEG and BAI physiological psychology tests. Test results found hyperactive beta waves present in all six patients' temporal lobe. The intensity of the wave of the right hemisphere temporal lobe T4(M=31.07) was the higher than that of the left hemisphere temporal lobe 3(M=29.11). Following Neurofeedback training, the beta wave of the right hemisphere lobe was reduced significantly in all patients. The average anxiety level decreased from 23.57 to 12.14 after the neurofeedback training. In addition, among the two patients who were taking medication, one patient reduced his dosage while the other patient discontinued taking medication. This result implies that the QEEG neurofeedback training technique can be effectively applied to patients with anxiety disorder.

• Microbiology and Biotechnology Letters
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• v.21 no.6
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• pp.582-587
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• 1993

The extracellular alpha-amylase was purified to homogenity from the culture filtrate of starch grown Sch. castellii CBS 2863. The purified enzyme was glycoprotein with a molecular weight of about 56 kDa. The pH and temperature optimum were 5.5 and 40C, respectively. The enzyme was fairly stable up to 40C and at acid pH range (pH 4.0-7.0). The apparent Km and Vmax of the enzyme toward starch was 1.0mg/ml and 100U/mg protein, respectively. The analysis of amino acid composition was found to be acidic protein. The amino acid sequence of N-terminal peptide consisted of Asp-Val-Ser-Ser-Ala-X-X-Thr-Arg-Ser-Glu-Ser-Ile-Tyr.