Korean Journal of Microbiology (미생물학회지)
- Volume 30 Issue 6
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- Pages.519-523
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- 1992
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
Purification and Properties of Glucoamylase form Yeast Candida tsukuaensis
- Kim, Sanga-Moon (Department of Spectacles and Optics, Kwang-ju Health Junior College) ;
- Bai, Suk (Department of microbiology, College of Natural Sciences, Chonnam National University) ;
- Chung, Hee-Young (Department of microbiology, College of Natural Sciences, Chonnam National University) ;
- Park, Jong-Chun (Department of microbiology, College of Natural Sciences, Chonnam National University) ;
- Lee, Jin-Jong (Department of microbiology, College of Natural Sciences, Chonnam National University) ;
- Kim, Dong-Ho (Department of microbiology, College of Natural Sciences, Chonnam National University) ;
- Song, Myoung-Hee (Department of microbiology, College of Natural Sciences, Chonnam National University) ;
- Chun, Soon-Bai (Department of microbiology, College of Natural Sciences, Chonnam National University)
- Published : 1992.12.01
Abstract
The glucoamylase of Candida tsuubaensis was purified to homogeneity form culture filtrate by means of ultrafiltration, Sephacryl S-200 gel filtration and Sp-Sephadex C-50 chromatography. The purified enzyme was a glycoprotein with a molecular mass of approximately 50 kDa, which was a monomeric protein. Km values were 5.8 mg/ml for soluble starch and 0.04 mM for maltose. Glucoamylase also released only glucose from both pullulan and isomaltose. The analysis of amino acid composition revealed that the enzyme contained a high content of acidic and polar amino acids. In addition, Western blotting analysis indicates that C. tsukubaensis glucoamylase is resistant to glucose repression.