• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.6
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• pp.869-874
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• 1998

A feeding trial was carried out to investigate the optimum density of juvenile abalone (Haliotis discus hannai) fed the formulated diet or macroalgae ( Undaria). Abalone with initial average shell length of 8.41 mm were stocked into three replicate tanks at four different densities of 1430, 2860, 4290, and 5720 individual/$m^2$ for 15 weeks. The formulated diet produced higher body weight and shell growth, soft body weight and survival rate than Undaria in abalone. Abalone fed the formulated diet have significantly (P<0,05) higher lipid and ash, and lower moisture and protein in soft body. Higher density resulted in lower growth and survival (P<0.05). Whereas shell growth, survival rate and weight gain were not significantly (P>0.05) different within the abalone stocked at stocking densities of 1430 and 2860 individual/$m^2$ in each diet group. Body composition of abalone was not affected by increasing density. These study indicate that the number of abalone (shell length ranges of between 8 mm and 17 mm) below 2860 individual/$m^2$ were predicted to effective density in the indoor tank culture system.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.36 no.2
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• pp.100-106
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• 2003

This study was conducted to investigate the supplementary effects of vitamin C and E to moist pellet on growth and body composition in juvenile olive flounder (Paralichthys olivaceus). Four groups of diet made : moist pellet (MP) diet group was used as the control group where the ratio of raw feed and commercial compound meal is 5:5 $(CP\;30\%,\;CL\;17\%),$ vitamin C added group (VC), vitamin I added group (VE) and vitamin C and I added group (VCE). The supplementary amount of vitamin C and E corresponded to 1000 mg/kg (dry wt.) and 220mg/kg (dry wt.), respectively. Vitamin C was destroyed $50\%$ and vitamin E was destroyed $20\%$ for manufacturing process. After 8 weeks feeding trial, weight gain was $121.9\%$ in MP group, while it ranged from 180.5 to $184.9\%$ in the VC, VE and VCE group. Feed efficiency was $71.\6%$ in MP group, whereas it ranged from 78.7 to $80.6\%$ in the VC, VE and VCE group, weight gain and feed efficiency of fish fed vitamin supplemented diets were significantly higher than control group and no significant difference among the vitamin added groups was observed (p>0.05). There were no significant differences in moisture, crude protein, crude lipid, crude ash, condition factor (CF), hepatosomatic index (HSI), and visceraweight index (VWI) of body composition. Hemoglobin (Hb) was $3.79\%$ in MP group, whereas $4.99\%$ in VC group, $4.55\%$ in VE group and $5.07\%$ in VCE group; Hb was significantly higher in the vitamin added groups than the control group (p<0.05). Vitamin C retention in liver was 18.1, 20.7, 46.6, 54.0 mg/kg in MP, VE, VC and VCE groups, respectively. Vitamin E retention in liver was 25.9, 53.8, 88.2, 124.5 mg/kg in MP, VC, VE and VCE groups, respectively. These results indicate that supplementation of vitamin C and E to moist pellet diet significantly improved growth and feed efficiency of olive flounder, and the mixed addition of vitamin C (1,000 mg/kg dry wt.) and vitamin E (220 mg/kg dry wt.) did not lead to the synergy effect.

• Fisheries and Aquatic Sciences
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• v.19 no.8
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• pp.32.1-32.6
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• 2016

A feeding trial was conducted to evaluate the effects of two different sizes of extruded pellets (EP) ($EP_1$ - 3 mm or $EP_2$ - 5 mm) and a moist pellet (MP) in olive flounder, Paralichthys olivaceus, reared in semi-recirculation system. A total of 450 fish with an average initial weight of $5.0{\pm}0.2g$ (mean ${\pm}$ SD) were fed one of the three experimental diets in triplicate groups. At the end of a 6-week feeding trial, weight gain, specific growth rate, and feed efficiency of fish fed EP diets were significantly higher than those of fish fed MP (P < 0.05). Water quality parameters like turbidity, total ammonia nitrogen, and total phosphorous from tanks of fish fed $EP_1$ and $EP_2$ were significantly lower than those from tanks of fish fed MP. Blood plasma glutamic oxaloacetic transaminase and glucose concentration were significantly higher in fish fed MP diet compared to fish fed EP diets (P < 0.05). Whole body crude protein contents in fish fed EP diets were higher than those from the fish fed MP diet. Whole body amino acid content like threonine, aspartic acid, serine, tyrosine, and cystine were found to be significantly higher in fish fed EP diets than those in fish fed MP diet. In considering overall performance of olive flounder, $EP_2$ diet could be recommended for the successful aquaculture of this important fish species.

• Fisheries and Aquatic Sciences
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• v.23 no.4
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• pp.7.1-7.8
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• 2020

Background: To minimize the use of antibiotics and to obtain a more sustainable fish culture and aquaculture industry, development of alternative natural source of immunostimulant to replace antibiotic in aquafeed is highly needed. Objective: Dietary inclusion effect of yacon (YC), ginger (GG), and blueberry (BB) on growth, body composition, and disease resistance of black rockfish against Vibrio anguillarum was compared to ethoxyquin (EQ). Methods: Four hundred eighty juvenile (an initial weight of 4.2 g) fish were randomly distributed into 12 of 50 L flowthrough tanks (forty fish per tank). Four experimental diets were prepared; the control (Con) diet with 0.01% EQ inclusion, and YC, GG, and BB diets at 1% each additive inclusion. Each additive was included into the experimental diets at the expense of wheat flour. Each diet was assigned to triplicate tanks of fish and hand-fed to satiation twice daily for 8 weeks. At the end of 8-week feeding trial, 20 fish from each tank fish were artificially infected by intraperitoneal injection with 0.1 mL of culture suspension of pathogenic V. anguillarum containing 3.3 × 10⁶ cfu/mL respectively. Fish were monitored for the following 8 days after V. anguillarum infection and dead fish were removed every 6 h for the first 4 days and 12 h for the rest of the study. Results: Weight gain, specific growth rate (SGR), and feed efficiency ratio (FER) of fish fed the YC diet was higher than those of fish fed all other diets. However, feed consumption, protein efficiency ratio, and protein retention was not affected by dietary additive. Moisture, crude protein, and crude lipid content of the whole body of fish were affected by dietary additive. Analysis of the Kaplan-Meier survival curves showed that survival of fish fed the YC, BB, and GG diets was higher than the Con diet. Conclusion: Oral administration of YC can improve not only weight gain, SGR, and FER of black rockfish, but lower mortality of rockfish at occurrence of V. anguillarum.

• Journal of Aquaculture
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• v.16 no.4
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• pp.210-215
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• 2003

When fed on one of the six isonitrogenous (45%) and isolipic (8%) feed containing 5 or 10% Undaria, 2% wasabi leaf, 2% wasabi stem and 0.5% herb (Obosan) for a period of 8 weeks, 95-98% juvenile flounder survived. Growth, feed efficiency and condition factor of the flounder fed on diet containing 0.5% herb were significantly (p<0.05) higher than those fed on diet supplemented with 10% Undaria. There was no significant (p>0.05) differences in moisture, crude protein, lipid and ash of the flounder receiving the different diets. The flounder fed on diet supplemented with 10% Undaria had the highest moisture but the lowest lipid in liver. Hematological parameters such as red blood cell, hematocrit and hemoglobin and serum constituents such as glucose, total cholesterol and glutamic-oxaloacetic transaminase of the flounder fed on the diets varied but no specific trend became apparent. Lysozyme activity in the serum of the flounder fed on diet supplemented with 5% Undaria and the herb, as well as nitroblue tetrazolium (NBT) reduction of macrophage in the head kidney of the flounder fed on diet containing the herb and 2% wasabi stem were significantly (p<0.05) higher than those receiving control diet. Briefly, the herb supplementation promoted growth and that of Undaria and wasabi stem enhanced non-specific immune response.

• Journal of Aquaculture
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• v.17 no.4
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• pp.235-239
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• 2004

Experiments were carried out to examine the effects of the different levels of oxygen, ammonia and hydrogen sulfide on survival, specific growth rate (SGR) and feed conversion ratio (FCR) of juvenile shrimp, Fenneropenaeus chinensis. Survival of the shrimp exposed to the hypoxia with $\leq$2.5 mg/L dissolved oxygen for 24 days were significantly affected. SGR and FCR of the shrimp exposed to$\leq$3.0 mg/L dissolved oxygen were significantly reduced than those of shrimp reared at 6.8 mg/L dissolved oxygen concentration. Survival of the shrimp exposed to $\geq$2.0 mg/L of ammonia levels for 24 days was significantly affected. Moreover, compared to the control group significant decrease of SGR and FCR of the shrimp has been observed with $\geq$1.0 mg/L and $\geq$0.5 mg/L of ammonia concentrations. In case of hydrogen sulfide, $\geq$0.5 mg/L was the critical level showed its significant negative effect on survival rate of shrimp exposed for 24 days. While, the group exposed with $\geq$0.07 mg/L and $\geq$0.05 mg/L hydrogen sulfide levels had a lower SGR and FCR values than did the control group in the same stipulated time of exposure.

• Journal of Aquaculture
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• v.18 no.2
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• pp.98-106
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• 2005

A feeding trial of two extruded pellets (EPI, 45% crude protein with 7% crude lipid and EP2, 47% crude protein with 10% crude lipid)${\times}$ two satiation rates (satiation and 80% satiation)${\times}$ three feeding frequencies (three, two and one meal a day) factorial design with three replications was carried out to investigate the optimum feeding frequency and satiation rate for growth of juvenile flounder. Flounder averaging 55 g were fed a extruded pellets for 58 days. Daily feed intake was affected by dietary composition, feeding frequency and satiation rate (P<0.05). Weight gain of fish fed the EP1 and EP2 was not affected by both dietary composition and feeding frequency (P>0.05), but affected by feeding satiation rate (P<0.05). Weight gain of fish fed the each of EP1 and EP2 twice daily with satiation was the highest among treatments, but was not significantly different to that of fish fed the diets three times daily with satiation feeding. Feed efficiency and protein efficiency ratio of the fish were not affected by dietary composition, feeding frequency and satiation rate (P>0.05). Moisture, crude protein and crude lipid contents of the muscle of fish were affected by satiation rate. Moisture and crude protein contents of the liver were affected by dietary composition. Based on fish performance results, it can be concluded that the proper feeding frequency for the growth of juvenile flounder grown from 55 g to 90 g is two meals a day with satiation feeding, regardless of dietary composition used in this study.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.42 no.1
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• pp.56-62
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• 2009

Feeding regimens was investigated for the growth and survival rate of juvenile Maroon clownfish (Premnas biaculeatus) in order to establish a seed production technique for this fish. Three types of feeding regimens were used, and growth and survival were highest under the following regimen: rotifer from day 0, Artemia from day 4, copepod from day 9 and artificial food from day 15, respectively (P<0.05). The average body lengths of the larvae were $6.76{\pm}0.55\;mm$, $7.63{\pm}0.50\;mm$ and $7.33{\pm}0.43\;mm$ in the Nannochloropsis oculata, Isochrysis galbana and control treatments conducted with 3 larvae/L, respectively (P<0.05). There were significant differences in larval growth between the treatments and controls conducted with 6 larvae/L and 9 larvae/L, respectively (P<0.05) but there was no significant difference between the N. oculata and I. galbana treatments (P>0.05). There were no significant differences in larval growth between the treatments at densities of 3-9 larvae/L (P>0.05). At highest density level, the larvae reared in I. galbana had the highest survival rate whereas survival was lowest in the control.

• Environmental Analysis Health and Toxicology
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• v.22 no.1 s.56
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• pp.83-90
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• 2007

This study was investigated the effort of growth and gonadal development in olive flounder, Paralichthys olivaceus exposed to PCBs Effluent Allowance Concentration, 3.0 ppb in Korea. Total length, body weight and weight gam of fish were no significant differences between two treatments (control: T.L.-110.0 mm, T.W.-9.61 g: treatment: T.L.-112.8 mm, T.W.-9.68 g), however, weight gain of fish unexposed to PCBs wat observed to increase gradually from 100 days after hatching (30 days after treatment). In the indifferentiated gonad to the juvenile stage, the differentiation of the ovary occured at 60 days after hatching (20 days after treatment) and that of the testis occured at 70 days after hatching (30 days after treatment) in two groups. In the ovary, the oogonia and ovarian lamellar appeared 130 days after hatching (50 days after treatment). The spermatogonia and resticular lobule of the testis were observed at 130 days after hatching (50 days after treatment). Testicular cyst appeared 140 days after hatching (60 days After treatment). There was no difference on the development of ovary and testis. There was no obvious difference of sex ratios. Therefore, These results considerate that PCBs have any effect for growth and gonadal development, but a little act for early growth in olive flounder.

• Journal of Aquaculture
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• v.11 no.1
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• pp.83-90
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• 1998

The effects of Obosan in the diet on growth, percent weight gain, specific growth rate, feed conversion ration and body composition of nile tilpia were investigated. First feeding fry average weighing 11.0 mg were fed the diets containing 0%, 0.25%, 0.5%, 1.0% and 2.0% Obosan for 7 weeks. Body weight, percent weight gain, specific growith rate of fish fed the diets containing 0.25%, 0.5% and 1.0% Obosan were significantly higher than control diet (P<0.05). Feed conversion ratio of fish fed the diets containing 0.25% and 0.5% Obosan showed best results compare to other experimental groups (P<0.05). Juvenile average weighing 281.0g and adult fish average weighing 563.0g were fed control and 0.3% Obosan supplemented diet for 30 days and 175 days, respectively. Body weight, percent weight gain and specific growth rate were significantly higher in the fish fed Obosan supplemented diet than control diet (P<0.05). Crude protein, crude lipid, crude ash and moisture of dorsal muscle and liver were not significantly different between two experimental groups.