• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.46 no.6
• /
• pp.769-776
• /
• 2013

This study evaluated fermented soybean meal (FSM) as a fish meal (FM) replacement and determined the appropriate amount of FSM in juvenile olive flounder diet. Twenty-four aquaria with a flowing-water system were stocked with fish averaging 20.9 g at a density of 25 fish/tank. Five experimental diets were prepared replacing FM with 0, 10, 20, 30, or 40% FSM based on FM protein (designated $FSM_{0}$, $FSM_{10}$, $FSM_{20}$ $FSM_{30}$, and $FSM_{40}$, respectively). Two additional diets were prepared that replaced 30 or 40% of the FM with FSM with added amino acids (methionine and lysine) (designated $FSM_{30+AA}$, and $FSM_{40+AA}$, respectively). Fish (triplicates) were fed one of the eight experimental diets (50% crude protein and 16.7 kJ available energy $g^{-1}$ diet) for 8 weeks. Survival did not differ among the treatments during the feeding experiment. There were no significant differences in weight gain (WG) or specific growth rate (SGR) among the fish fed diets with up to 30% of the FM replaced. However, fish fed $FSM_{40}$ or $FSM_{40+AA}$ had a reduced WG and SGR, as compared to $FSM_0$ (control) (P < 0.05). The feed efficiency and apparent digestibility showed a similar trend (P < 0.05). The proximate composition in the whole body of fish differed only between the control and $FSM_{40}$ for the crude protein level and between the control and $FSM_{30+AA}$ for the crude lipid level. The whole-body amino acid composition did not differ among treatments. No significant differences were found between the diet groups with and without amino acid supplementation, indicating that amino acid supplementation had no effect. The major finding of this study is that fermented soybean meal may replace up to 30% of fish meal without amino acid supplementation for normal growth of juvenile olive flounder.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.46 no.6
• /
• pp.761-768
• /
• 2013

We conducted two feeding trials to investigate the optimal feeding frequency of juvenile Korean rockfish Sebastes schlegeli fed a commercial diet of expanded pellets containing 47.2% crude protein, 9.2% crude lipid, and 14.5% ash at two different water temperatures. In the first experiment, triplicate groups of 20 fish with an average weight of 2 g were fed an equal amount of diet (5.97% based on body weight) at one of six feeding frequencies (2, 3, 4, 5, 7, or 9 meals/day) for four weeks at $17.5^{\circ}C$. After four weeks, we measured weight gain (WG), specific growth rate (SGR), feeding efficiency (FE), and protein efficiency ratio (PER). Fish fed diet five meals/day grew significantly better than those fed nine meals/day. The second experiment used identical experimental conditions and feeding regions, except the food ration was slightly less (5.92% based on body weight) and the water temperature was increased to $20^{\circ}C$. After four weeks, we again measured WG, SGR, FE and PER. Fish fed seven meals/day grew significantly faster than those fed 2, 3, 4, or 9 meals/day. Whole-body protein levels in fish fed three meals/day was higher than those fed four meals/day in $17.5^{\circ}C$ water, but whole-body lipids in the fish fed four meals/day was higher than those fed two meals/day in $20^{\circ}C$ water. A second-order polynomial analysis based on WG suggested the optimal feeding frequency for juvenile Korean rockfish was five meals/day at $17.5^{\circ}C$ and six meals/day at $20^{\circ}C$, indicating that fish reared in higher water temperature require higher feeding frequencies.

• Journal of Aquaculture
• /
• v.10 no.3
• /
• pp.255-260
• /
• 1997

In order to investigate the effects of phosphorus and iron levels in the diets containing fish meal as only protein source on the growth, feed conversion, body composition and blood characteristics of juvenile Korean rockfish, either graded monobasic potassium phosphate (MPP) of 1% (MPP-1), 2% (MPP-2), 3% (MPP-3) and 4% (MPP-4), or Fe-citrate (FC) of 0.022% (FC-1), 0.044% (FC-2), 0.066% (FC-3) and 0.088% (FC-4) was added to fish meal-based diet (MMP-0 or FC-0), respectively. Duplicate groups of 40 fish weighing 7g were fed the diets for 21 weeks. Weight gain, feed efficiency and nutrient retention of fish fed diets with different MPP levels were not significantly different (P>0.05). Lipid content of whole body decreased with an increase in dietary MMP (P<0.05). No significant differences were found in the Ht, Hb, total protein, or total glucose in serum among diets containing different MMP levels (P>0.05). Weight gain of fish fed the FC-0, FC-1 adn FC-3 diets was significantly higher than that of FC-4 diet (P>0.05), whereas feed efficiency, nutrient retention, hematological values (Ht, Hb, total protein, and total glucose), and whole body composition were not affected by dietary FC levels (P>0.05). The present results revealed that phosphate or iron supplementation to the diet containing 65% fish meal for juvenile Koran rockfish was not necessary.

• Fisheries and Aquatic Sciences
• /
• v.21 no.9
• /
• pp.26.1-26.8
• /
• 2018

Inclusion effect of soybean meal (SBM) and fermented SBM (FSM) in extruded pellet for juvenile abalone (Haliotis discus) was compared in abalone farm. Dietary inclusion effect of the combined macroalgae (MA) (Undaria pinnatifida and Hizikia fusiforme) and a single Saccharina japonica on abalone was also compared. Three thousand six hundred juvenile abalone were purchased from a private hatchery and acclimated to the experimental conditions for 2 weeks. Six 5-ton flow-through raceway tanks were used, and abalone were randomly distributed into tanks (n = 600 per tank). Three experimental diets were prepared in duplicate. Fish meal, FSM, corn gluten meal, and shrimp meal and wheat flour and dextrin were used as the protein and carbohydrate sources, respectively, in the FSM diet. MA was also included in the FSM diet. FSM and MA in the FSM diet were substituted with SBM at the expense of wheat flour and S. japonica, referred to as the SBM and SJ diets. The experimental diets were pelletized by an extruded pelleter. Water stability of nutrients in the experimental diets was monitored at 12, 24, and 48 h after seawater immersion. The experimental diets were fed to abalone once a day to satiation with a little leftover for 120 days. The retained crude protein and lipid and ash content of the extruded pellets were changed over all period of time. Weight gain and specific growth rate (SGR) of abalone fed the SBM diet were greater than those of abalone fed the FSM and SJ diets. Weight gain and SGR of abalone fed the SJ diet were also greater than those of abalone fed the FSM diet. The longest shell length, widest shell width, highest shell height, and greatest soft body weight were obtained in abalone fed the SBM diet, followed by the SJ and FSM diets. Proximates of the soft body of abalone were not different among the experimental diets. In conclusion, SBM was a superior protein source to FSM in extruded pellet for growth performance of abalone. Dietary inclusion of a single S. japonica was superior to the combined inclusion of U. pinnatifida and H. fusiforme in the production of abalone.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.47 no.6
• /
• pp.824-831
• /
• 2014

This study was conducted to investigate the effects of fishmeal replacement with acid-concentrated soybean meal (ACSBM) on growth performance, blood biochemistry, and ingredient digestibility in juvenile olive flounder Paralichthys olivaceus. Six experimental diets were formulated to replace fishmeal protein with ACSBM at 0%, 20%, 30%, 40%, 50%, and 60% (designated ACSBM0, ACSBM20, ACSBM30, ACSBM40, ACSBM50, and ACSBM60, respectively). Triplicate groups of fish (initial fish mean weight: $14.3{\pm}0.03g$) were fed the experimental diets to apparent satiation (twice daily at 08:00 and 18:00 h). After a 12-week feeding trial, a total of 180 healthy fish were randomly distributed into three Guelph system tanks at a density of 60 fish/tank (initial fish mean weight : $50.6{\pm}2.4g$) to test the apparent digestibility coefficients of the ingredients (ACSBM, fishmeal, and soybean meal). Although negative effects were observed with ACSBM40, ACSBM50 and ACSBM60 after 12 weeks of feeding, up to 20% of the fishmeal protein could be successfully replaced with ACSBM without significant growth depression. Hemoglobin and hematocrit values of fish fed the ACSBM50 and ACSBM60 diets were significantly lower than those of fish fed the ACSBM0 diet. Glucose values of fish fed the ACSBM60 diet were significantly higher than those of fish fed the ACSBM0 and ACSBM20 diets. Digestibility of protein in ACSBM and soybean meal was 85.9% and 82.5%, respectively. Results indicated that at least 20% of fishmeal protein can be replaced by ACSBM in diets of juvenile olive flounder without supplementation of limiting amino acids.

• The Journal of the Korea Contents Association
• /
• v.13 no.8
• /
• pp.152-159
• /
• 2013

Delinquency young people committed society as evolves is also increasing rapidly. Juvenile delinquency that occurred recently is serious. As a result, young people are feeling fear of delinquency. Fear of delinquency young people feel, a major impact on the personality development of the youth of the period of growth. In particular, the violent delinquency, to give a physical impact, damage youth receives is large. As well as physical damage to people in and around the juvenile victims, given the damage mental serious form, fear violent delinquency has been shown in many previous studies. Therefore, in this study you have the purpose to study the effects on the reaction of juvenile victims for fear of delinquency caused by the delinquency of violence. Set randomly on campus and external experience and damage directly or indirectly, are classified into action evasive action defensive reaction of fear by delinquency, factors that affect the risk caused by violent delinquency, Daegu after conducting a survey of high school students in general in the areas in which we tested the hypothesis. As a result of the verification, exerting a significant influence in order to act defensive becomes clear, experience of direct damage and experience of damage indirect fear of delinquency, avoided experience of direct damage it was found to exert a significant effect on to the action manner.

• Journal of Aquaculture
• /
• v.19 no.2
• /
• pp.125-132
• /
• 2006

Black rockfish larvae and juveniles were reared for 95 days after parturition (DAP) in order to determine four enzyme activities (trypsin, pepsine-like enzyme, lipase, amylase) during ontogeny. Larvae were fed rotifers Brachionus plicatilis from 1 to 25 DAP, Artemia nauplii from 10 to 78 DAP and then gradually changed to pelleted feed from 30 DAP. Temperature was kept between $13.5{\sim}14.9^{\circ}C$. Trypsin and lipase activities were found in 2 DAP larvae ($7.0{\pm}1.5$ unit and $4.5{\pm}1.4$ unit, $mean{\pm}SD$, respectively). The evolution of both enzymes activities showed a profile marked by drastic increases between postflexion and juvenile stage. There is an increment on specific trypsin activity at 10 DAP, corresponding with the beginning of Artemia feeding. Pepsin-like enzyme activity was found at 11 DAP and increased drastically from 56 DAP, cor-responding with the initiation of juvenile stage. Amylase activity was also found at 11 DAP and maintained at a low level up to 38 DAP followed by a drastic increase from 39 DAP to 50 DAP. Considering our results of both trypsin and pepsin-like enzyme activities, it might be concluded that higher somatic growth of Sebastes inermis could be possible with the initiation juvenile of stage and the early juvenile stage is a suit-able period for feeding an artificial diet for fish.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.59 no.2
• /
• pp.181-187
• /
• 2014

Polycyclic aromatic hydrocarbons (PAHs) are a group of ubiquitous hazardous pollutants derived from fossil fuel, various combustion sources and pyrolysis of a wide range of plastics. Because PAHs can be uptake into crop plants, the inhibitory effects on rice and soybean plants were examined in greenhouse and growth chamber experiment. Soil-applied PAHs (phenanthrene of 0, 10, 30, 100 ppm) slightly reduced the plant height and dry weight both in transplanted rice and soybean plant. The inhibitory effect on growth was greater in soybean than rice. Plant height of soybean plants treated by 100 ppm was 58.9 cm and this value was 87.2% of untreated plant. In rice plant, the plant height was less inhibited (96.0% of untreated plant) by 100 ppm at 80 days after treatment (DAT). However, leaf chlorophyll content and chlorophyll fluorescence were less inhibited by PAHs at late growth stage (after heading) although the photosynthesis-related parameters were slightly inhibited from 20 DAT to 70 DAT. In agar medium experiment with infant seedlings, inhibition of seedling length and fresh weight by phenanthrene at 100 ppm were greater as compared to the experiment with adult plant in pot. Seedling length and fresh weight were reduced by 54.2% and 33.3% for rice and 27.9% and 13.2% for soybean, respectively. The results reflected that PAHs were more inhibitory during juvenile stage than adult stage and more inhibitory to rice plant than soybean for juvenile stage.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.32 no.3
• /
• pp.290-294
• /
• 1999

An 18-week growth trial was conducted in flow-through aquarium system to investigate the effects of several additives in formulated diets on the growth and body composition of juvenile abalone (Haliotis discus hannai). Four replicate groups of abalone averaging 150 mg were fed one of four isonitrogenous ($33.8\%$) and isolipidic ($5.8\%$) diets containing $5\%$ squid meal, $0.5\%$ attractant, $1\%$ herb or $0.5\%$ lecithin. In addition, these formulated diets were compared to commercial diet. Survival rate and shell growth of abalone were not significantly affected by the different dietary additives and commercial diet (P>0.1). Body weight gain of abalone fed the diet containing herb was higher than those of abalone fed the diets containing squid meal or lecithin and commercial diet (P<0.1). Moisture, protein, lipid and ash contents of soft body were not influenced by dietary additives and commercial diet (P>0.1). The results suggest that herb as additive in this formulated diet can improve weight gain of abalone.

• Journal of Aquaculture
• /
• v.21 no.1
• /
• pp.1-6
• /
• 2008

We determined the effects of dietary supplements for lactic acid bacteria(LAB) such as Lactobacillus brevis(Lb) and Lactobacillus plantarum(Lp) in juvenile rockfish Sebastes schlegeli cultured in flow-through system for 10 weeks. The experimental diets contained $10^4cfu/g,\;10^6cfu/g\;and\;10^8cfu/g$ level each LAB(Lb-4, Lb-6, Lb-8 or Lp-4, Lp-6, Lp-8), respectively. The effects of LAB supplementation was determined by various factor such as weight gain(WG), specific growth rate(SGR), feeding efficiency(FE) and blood assay. For rearing experiment, Lp-8 treatment had significantly high growth rate than control diet treatment. However, all Lb treatment had no significance effect with control diet treatment. In case of the blood assay, hematocrit(Ht) and hemoglobin(Hb) of fish were not affected by LAB supplemental levels. On the other hand, total cholesterol in plasma of Lb-8, Lp-6 and Lp-8 treatments were significantly low than the control diet treatment. We verified the influence of LAB which was originated from species specificity and amount in diet. Consequently, the dietary supplementation as $10^8cfu/g$ level of L. plantarum could be of help for growth enhancement to the juvenile rockfish.