Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
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Effects of Dietary Supplement of Probiotics on Growth and Blood Assay of Rockfish Sebastes schlegeli

생균제 첨가사료가 조피볼락 Sebastes schlegeli의 성장 및 혈액성분에 미치는 영향

  • Published : 2008.02.25
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Abstract

We determined the effects of dietary supplements for lactic acid bacteria(LAB) such as Lactobacillus brevis(Lb) and Lactobacillus plantarum(Lp) in juvenile rockfish Sebastes schlegeli cultured in flow-through system for 10 weeks. The experimental diets contained $10^4cfu/g,\;10^6cfu/g\;and\;10^8cfu/g$ level each LAB(Lb-4, Lb-6, Lb-8 or Lp-4, Lp-6, Lp-8), respectively. The effects of LAB supplementation was determined by various factor such as weight gain(WG), specific growth rate(SGR), feeding efficiency(FE) and blood assay. For rearing experiment, Lp-8 treatment had significantly high growth rate than control diet treatment. However, all Lb treatment had no significance effect with control diet treatment. In case of the blood assay, hematocrit(Ht) and hemoglobin(Hb) of fish were not affected by LAB supplemental levels. On the other hand, total cholesterol in plasma of Lb-8, Lp-6 and Lp-8 treatments were significantly low than the control diet treatment. We verified the influence of LAB which was originated from species specificity and amount in diet. Consequently, the dietary supplementation as $10^8cfu/g$ level of L. plantarum could be of help for growth enhancement to the juvenile rockfish.

조피볼락 Sebastes schlegeli 치어의 성장률 향상을 도모하고 적용할 만한 생균제로서의 그 가능성을 보고자, 평균 체중 0.7 g의 조피볼락 치어에 2종의 유산균을 사료에 첨가하여 본 실험을 수행하였다. 각각의 첨가구는 Lactobacillus brevis 및 Lactobacillus plantarum을 농도별로 실험사료 1 g 중량당 유산균 첨가수준을 $10^4,\;10^6$ 및 $10^8$ cfu 수준으로 처리한 첨가구로 하였다. 성장효과에서 L. plantarum을 농도별로 실험사료 1g 중량당 유산균 첨가수준을 $10^8$ cfu 수준으로 처리한 Lp-8 실험구에서 중량 증가 효과가 있었으며, L. brevis의 경우는 첨가효과가 없었다. 실험종료 후, 혈액분석 결과는 hematocrit 28.5-32.5%, hemoglobin 8.3-8.6 g/dl으로 차이는 없었으나, 혈장 내 총 단백질 함량은 Lp-8 실험구에서 높았다. 혈장 내 총 콜레스테롤 함량은 유산균 2종에서 높은 첨가농도 일수록 그 수치가 낮아지는 경향을 보였다. 상기 실험결과로 미루어 볼 때, 수중환경에 서식하는 어류의 장내 정상적인 균주가 아닌 유산균은 개별적 종에 따라 다른 효과를 얻을 수는 있으나, 조피볼락 치어는 L. plantarum을 $10^8$ cfu/g 수준으로 사료 내 첨가하여 성장효과를 거둘 수 있었다.

Keywords

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