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Homologous and Heterologous Antibody Response of the Patients with Aspergillosis Against Young Mycelia of Aspergilli by Fluorescence Antibody Reaction (형광항체반응을 이용한 Aspergillus 증 환자의 균사표면항원에 대한 항체반응 양상에 관한 연구)

  • Moon, Hi-Joo;Kwon, Hyuk-Han
    • The Korean Journal of Mycology
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    • v.17 no.2
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    • pp.82-90
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    • 1989
  • Detection of antibody against pathogenic fungi in serum specimens of the patients with pulmonary tuberculosis or other lung diseases has been carried out(male) using the indirect fluorescence antibody technique and immunodiffusion tests. Immunodiffusion tests revealed that 104(36.5%) out of 285 patients examined showed a positive precipitin reaction against one or more of fungal antigens. The majority of ID positive patients 64(61.5%) reacted with Aspergillus fumigatus antigen and 49(47.1%) patients reacted with Candida albicans antigen ID positive reaction to A. fumigatus was found little more frequently among male patients, while Candida albicans reactors were found more frequently among female patients. Age distribution of ID positive reactors was high(49.1-43.3%) in age group of 40-59 years, but least or none in age group of less than 30 years. Age of fungal mycelium used as antigen did not effect sensitivity of the indirect flubrescence (IF) technique in detecting antibody to A. fumigatus. Antibody class against A. fumigatus that showed highest titer was IgG and thus FITC labeled anti-IgG immunoglobulin shoul be preferable. As relatively large amount of cell wall components of Aspergilli shared antigenically, a considerable cross-reaction was observed among A. fumigatus, A. flavus and A. niger, but not much with C. albicans. While (IF) has much better sensitivity when compared with ID, relative specificity of the latter procedure cannot to be overried, so that they could be batter used together in order to obtain quantitative measurement of antibody with relative specificity.

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Influence of Dichlorprop and MCPB on the Reduced Effect of Fruit Drop and Fruit Quality before and after Storage in Apples (생장조정제(生長調整劑) Dichlorprop 및 MCPB 처리(處理)가 사과의 후기낙과(後期落果) 경감효과(輕減效果) 및 저장후기(貯藏後期) 과실(果實)의 품질(品質)에 미치는 영향(影響))

  • Lee, H.S.;Kang, C.K.;Ryu, G.H.;Park, Y.S.;Jung, J.H.
    • Korean Journal of Weed Science
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    • v.11 no.2
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    • pp.128-136
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    • 1991
  • This experiment was conducted to evaluate the effect of plant growth regulators, dichlorprop and MCPB on the reduced effect of fruit drop and fruit quality before and after storage in apples. Dichlorprop was tested with dilution of 1000 at 30, 40, 50 days before harvesting, and MCPB with dilution of 4000 at 15, 25, 35 days before harvesting. The results are summarized as follows : Percentage of fruit drop was appeared to the notable reduction as compared with the untreated control when regulators was applied with dilution of 1000 at 30 days before harvesting by dichlorprop and with dilution of 4000 at 35 days before harvesting by MCPB. Degree of fruit colour showed to the remarkable promotion at all the treatment of 30, 40, 50 days before harvesting by dichlorprop as compared with the untreated control. Sugar contents in flesh was increased a little at the treatment of 30 days before harvesting by dichlorprop, but acid contents in flesh was reduced at all the treatment of 30, 40, 50 days before harvesting by dichloroprop and at 15, 25, 35 days before harvesting by MCPB. Passed firmness of fruit after storage was maintained at the treatment with dilution of 4000 at 35 days before harvesting. Therefore, it was repressed a softening of fruit, but by dichlorprop treatment at 30, 40, 50 days before harvesting, fruit firmmess was appeared to reduce according to the passage of storage period. Amount of ethylene evolution after storage was showed to reduce at all the treatment by early treated time of dichoroprop and MCPB, but carbon dioxide increased at treatment conditions such as the front. Accordingly, these relationship showed to be contrary each other.

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Occurrence and Repression of Off-Odor in Cooked Rice during Storage under Low Temperature Warming Condition of Electric Rice Cooker (전기밥솥으로 저온보온한 쌀밥의 이취 발생 및 억제)

  • Park, Seok-Kyu;Ko, Yong-Duck;Cho, Young-Sook;Shon, Mi-Yae;Seo, Kwon-Il
    • Korean Journal of Food Science and Technology
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    • v.29 no.5
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    • pp.919-924
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    • 1997
  • The changes in physicochemical properties of cooked rice stored in an electric rice cooker at different temperature (63, 66, 69 and $72^{\circ}C$) were investigated. The growth of thermophilic bacteria was gradually increased in all samples with storage time up to 6 hours and increased rapidly afterward. The bacterial growth rate was higher during storage at low temperature than at high temperature, and the bacterial number generally reached the maximum at 18 hours of the storage. The number of bacteria in sample stored at $63^{\circ}C$ was increased to $10^6$ CFU/g after storage for 6 hours. The heat treatment at 6 hours of storage decreased the bacterial number to $10^5 $ CFU/g at 8 hours of storage. Bacterial number was gradually increased with storage time after the first heat treatment. When the sample was reheated after 8 hours of storage, the bacterial number was reduced to the level at which off-odor was not detected after storage for 24 hours. During the storage, moisture content of heat-treated sample was lower than that of sample stored at $63^{\circ}C$ but higher than that of sample stored at $72^{\circ}C$. The L value of heat-treated sample was higher than that of the sample stored at $72^{\circ}C$, but lower than that of the sample stored at $63^{\circ}C$. The b value showed an opposite trend to the L value with regard to the storage temperature. Changes in texture were not remarkable during the storage for the sample heat-treated and stored at low temperature. The occurrence of off-odor and browning was depressed in the heat-treated sample, and the texture and overall eating quality were more acceptable than the samples stored at low temperature.

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Effects of pH and Natural Polysaccharide Gums on the Foam Stability of Soy Protein Isolate (pH와 천연 다당류 고무질이 분리대두단백질의 거품안정성에 미치는 영향)

  • Yang, Seung-Taek;Kim, Mi-Sook;Park, Chun-Og
    • Korean Journal of Food Science and Technology
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    • v.24 no.5
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    • pp.482-491
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    • 1992
  • Experiments were conducted to elucidate the effects of pH change and sodium alginate, gum karaya and gum arabic on the foaming properties of soy protein isolate (SPI). The surface tensions of SPI solution (5%) adding to gums at pH 4.0 and 5.0, near the isoelectric point (pH 4.5) were higher than those at pH 7.0 and 8.0. Specific viscosity of the solutions adding to gums (0.1, 0.2 and 0.3%) at pH 7.0 and 8.0 were $3.6{\sim}51.8$ and $4.0{\sim}51.2$, respectively. In cases of addition of sodium alginates specific viscosity were predominantly increased, while those of gum arabic did not almost increase. Addition of gums reduced the overrun, but it stabilized the foams, especially those of sodium alginates (0.2%) increased the foam stability at pH 5.0, 7.0 and 8.0 by 57, 413 and 315%, respectively. Foaming ability, foam stability and heat stability of the foam were significantly (p<0.05) affected by changing pH. There was a tendency to increase the foaming ability according to the decrease of surface tension. Specific viscosity was also important in foam stability and heat stability.

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Gamma Irradiation Effects on Conchospores of Porphyra Species 2. The Effects of High Gamma Irradiation on Germination and Growth of Conchospores of Two Varieties (김의 각포자에 대한 r-선의 조사효과 2. 두 품종의 각포자의 발아생장에 미치는 고선량 r-선의 조사효과)

  • KIM Joong-Rae
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.18 no.1
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    • pp.52-56
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    • 1985
  • For the fundamental studies of radiation breeding in edible marine algae, the biological effects on conchospores of Porphyra species by gamma-irradiation were examined. Two varieties, Keun-cham-gim (Porphyra tenera Kjell. form tamatsuensis Miura) and Saga No.5, were chosen for this study, and their conchospores after r-irradiation($5.0{\sim}20.0$ KR) were cultured for 50 days. The results obtained were summarized as follows. 1. Gamma-irradiation in less than the dose of 20KR did not affect germination of conchospores, and almost all spores grew into two cells germ in 24 hours after irradiation, but withering germs were gradually increased in number according to higher exposure within 5 days old culture. 2. High irradiation caused the induction of giant cells, abnormal useless growth of hold-fast, lumpish thalli and callus-like lumpy tissues. 3. The liberation of neutral spores from young germs and carpospores from mature thalli were observed on the frond exposed at $10{\sim}20$ KR irradiation. All spores were normal in division and its size. 4. The best irradiation effect on growth of Keun-cham-gim was observed at 10 KR dose, whose growth-rates were $140\%$ in wet weight and $108\%$ in mean frond area, but only $48\%$ was recorded in wet weight at 20 KR exposure. Saga No.5 were in contrast with Keun-cham-gim, and their most growth-rate was $400\%$ in wet weight ($258\%$ in frond area) at 10 KR irradiation and the worst was $20\%$ at the dose of 20 KR. 5. The withering phenomenon to death by treatment of gamma-ray presented substantial difference between two varieties. Survival rate compared with control in Keun-cham-gim was $70.7\%$ at 20 KR, but that in Saga No.5 recorded $47.0\%$ at same dose. 6. Synthesizing the results of high and low r-irradiation, it was suggested tat high r-irradiation in more than 5.0 KR inhibited conspicuously the growth of germs derived from conchospores, and about half of them withered at 15.0 KR dose or more.

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Changes in Lipid Contents of the Tomato Fruits during Storage (도마도과실(果實)의 저장중(貯藏中) 함유지질(含有脂質)의 변화(變化)에 관(關)하여)

  • Lim, Young-Sook
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.15 no.1
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    • pp.27-38
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    • 1986
  • This experiment was conducted to observe the changes of $CO_2$ production, firmness and lipid components of tomato fruits at the temperature of $15^{\circ}C,\;20^{\circ}C\;and\;25^{\circ}C$ each. The phenomenon of climacteric rise appeared first at $25^{\circ}C$. The firmness of tomato fruits during storage were decreased rapidly under $25^{\circ}C$ but slowly under $15^{\circ}C$. The total lipid contents of tomato fruits were decreased under $15^{\circ}C\;and\;20^{\circ}C$ until the middle stage of storage but were increased rapidly at $15^{\circ}C$ from 30 to 40 days in storage. Various changes were not observed at the neutral lipid contents of tomato fruits. But. glycolipid and phospholipid contents were increased. The results which were observed the composite fatty acid of each lipid components were a lot of palmitic acid as a saturated fatty acid and linoleic acid. linolenic acid and oleic acid as an unsaturaturad fatty acid at the all fractions which were neutral lipid, glycolipid and phospholipid. The ratios of saturated fatty acid to unsaturated fatty acid of tomato fruits during storage were generally increased at the fraction of neutral lipid. tended to decrease at the fraction of glycolipid and were almost constant at the fraction of phospholipid.

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The Diagnosis for Educational Behavioral Strategies of Community Health Nurse-Community Health Worker for Control of Hypertensive Urban Young Black Men in America (간호사-지역사회건강상담자팀의 미국 도시지역 젊은 흑인 남자 집단의 고혈압 관리를 위한 전략 활동의 교육-행위진단)

  • Park, Kyung-Min
    • Research in Community and Public Health Nursing
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    • v.7 no.1
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    • pp.80-99
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    • 1996
  • Young black men(YBM) have the most severs levels of high blood pressure(HBP) and, in all reports but one, the lowest of HBP control of any age /sex /race group. To increase entry into care, remaining in care, and BP control for young(18-49 years) Black men, It is needed to review socio-demographic, medical characteristics, and behaviors(importance of and difficulty with HBP control behaviors, or worry about mdication) for experimental intervention study(educational- behavior strategies) of hypertensive urban young black men. The 204 participants had an average age of 38.8+7.0 years and an average educational level of $11.0{\pm}2.4$ years; only 23.1% were employed full- or part-time while 26% were on disability ; and 6% were married. Only 35.3% had an MD for HBP care and 37.3% had some form of health insurance. The average BP of those men currently being in care on medication(35.3%) was $148.2/95.1{\pm}19.5/11.3$ compared to those men not taking HBP care $153.7/99.1{\pm}14.0/9.8(p<.05)$. The average creatinine level was 1.3(excluding 3 marked elevations of 15.9, 9.6, and 7.7) for the 163 men consenting to have their blood drawn. Self-reported co-morbidity induded heart disease 7.8%, diabetes 8.9%, high cholesterol 18.2%, CVA 3.4%, alcohol and drug related problems 27.9% and 22.5% respectively. The kidney disease of those men currently being in care & on medication was 9.7 compared to those men not taking HBP care 0.8(p<.05). The problems of with sex life, physicl activity and dearly thinking of those men currently being in care & on medication was higher compared to those men not taking HBP care(p<.05). Questions of 'during the past month, on how many days did you have 5 or more drinks (bottles) of any alcoholic beverag?' and smoking of those men currently being in care & on medication was 18.1% and 72.2% compared to those men not taking HBP care 27.3 and 82.6%, respectively. HBP control behaviors was assessed with 1-5 point Likert subscales(5=extreme, 1-none at all), In general, th men reportd low levels of perceived psychological barrier to HBP care and control behaviors; importance of and difficulty with HBP control behaviors, or worry about mdication. For example, on a five point scale(1=none at all, 5=extreme), average ratings for perceived important and difficulty with BP care and behaviors were 2.8(SD=1.2) and 2.5(SD=1.1). Average ratings for perceived benefit with BP care and behaviors worry about medication of those men currently being in care on medication was 4.0(SD=0.9) and 2.2(SD=1.1) compared to those men not taking HBP care 3.6(SD=0.8), 2.8 (SD=1.6) respectively(p<.05). These data support the need for educational-behavioral strategies of community health nurse to improve high blood pressure control in this high risk group through perceived barriers to treatment, health care skills and use of resources, and social support.

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Feasibility of Application of Roy's Adaptation Model to Family Health Assessment (로이적응모델의 가족건강사정에의 적용가능성)

  • Jang Sun-Ok
    • Journal of Korean Public Health Nursing
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    • v.8 no.2
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    • pp.35-56
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    • 1994
  • This article was intended to survey whether Roy' Adapation model ('Roy Model') can be applied to family health assessment and to study whether application of the Roy Model to a Korean family is feasible. under the Roy Model, a family is viewed as an adaptation system having a series of process of input. process, feedback, and output. Further, the Roy Model indicates that a family contains Physiolosical, self-concept. role function and interdependent mode in respect of internal or external stimuli. In the event where the family health assessed, the adaptation mode of that family must be assess at the first stage. Then, the focal, contextual, residual stimuli affecting the family must be assessed. In 1984 Hanson suggested four types of family adaptation mode based upon the Roy Model and thereby enhanced the possibility for family health assessment. In order survey whether the Roy Model can be applied to the Korean family, the author of this article contracted adults of 169 who live in 'A' city to make open questions regarding family and then analyzed responses from them by utilizing Roy model. This study categorized family Adaptation mode based upon the' four types of family adaptation mode developed by Hanson. As a result of this study, family adaptation mode was categorized into 117 concepts. Those 117 concepts are consisted or Physiolosical mode of 47. self­concept mode of 56, role function mode of 9 and interdependent mode of 5. Further. stimuli affecting family were classified based upon Roy's definition as to three types of stimuli. Stimuli on a family are comprised focal stimuli concept of 19, contextual stimuli concepts of 19, one residual stimuli concept. this result implies that the Roy's Model can be applied to Korean family. Physiological mode shows meaning of survival. while self-concept mode reflects meaning of growth and emphasizes harmony among the family based on the familism. The role function mode shows continuity rather control of family member. By contrast, interdependent mode shows interaction with community to which the family belongs. but the degree of interaction does not appear too high. The analysis of family stimuli led this study to conclude that troubles within a family. changes in family structure and diease of family member generate stimuli. However, an application of the Roy Model contains the following problems: First, Roy argued that the family adaptation mode should be assessed at the first level family health assessment and then stimuli affecting family adaptation should be adaptation assessed at the second stage. To the belief of the author of this article. however, for checking family adaptation level. focal, contextual, residual stimuli should be confirmed by assessing stimuli at first stage. Then, the family adaptation mode in respect of such stimuli should be assessed. The rationale for this is that the family adaptation level is determined depending on degree of strength of focal. contextual. residual stimuli. Second. Whall (1991) raised a question 'Does one assess family adaptation mode and intervene in the stimuli?' 'Likewise, assessment of the family adaptation should be made in the following manner in order for family health to be enhanced. Third. Roy believes that additional stimuli (such as contextual and residual) are same as internal process (including nurturance. support, and socialization). However, the basis for this Roy's belief is not too clear. In spite of these problems which the author indicated above, it can be concluded that the Roy Model can serve as a good device for an assessment of family health and that the Roy Model can be applied to a Korean family. Finally, further research of family adaptation theory and family nursing theory is required for a development of these theories.

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Study on the Oral Health Care by Parents Who have The Child (자녀(子女)에 대한 구강(口腔) 보건(保健) 기초(基礎) 지식(知識)에 관한 연구(硏究) (대구시내(大邱市內) 치과내원(齒科內院) 환자(患者)의 부모(父母)를 중심(中心)으로))

  • Lee, Jong-Do
    • Journal of Technologic Dentistry
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    • v.19 no.1
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    • pp.175-184
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    • 1997
  • The purpose of this study is to examine the effect of parents knowledge on oral health status toward their children. This study was carried out to investigate and analyze the congnitive degree of dental subjects and public who have an experience to be treated for their teeth on the dental knowledge, operative dentistry, and dental prothesis in the oral health care through the questionnaires. The results are as follows; 1. In questioning the fundamental knowledges, 47.6% of respondents in their thirties said that milk tooth of their children are number 20. Although schooling level of their parents is not rel evant to this survey, 21.9% who have only high-school diploma, said also "number 20". As a conclusion, most parents have a big concern in their children's teeth. 2. 44.0% parents answered, asked "who do decayed teeth sping up, and 26.4% said" It is why children eat sugary. Asked "What is efficient preventive measures decayed teeth", 26.5% said it is very relevant to be in the utilization of F-tooth-paste. 3. For children's good theeth, periodically brushing is the important. Most parents recognized brushing efficient. 4. In questioning the possession amount of decayed teeth, 45.8% have more than four. Usually those parents who have children filled their decayed teeth with artificial or installed with dental prosthesis. As a conclusion age is relevant in this study in P<0.05 level. 5. In asking for efficient starting-year of tooth brushing, 42.6% said that 2 or 3 year age is the most efficient, and especially 80.6% teath children to learn brushing themselves(P<0.05) 6. In surveying the way they obtained dental information, 38.9% were given from mass media, but 90% were once not delivered with the education of dental health. Because parents have been know narrow and deficient information of dental health mass media, experts in dental health have to deliver help their parents to be delivered with systematical dental information. The conclustion of this study can summarized that parent's role very important to their children's tooth health and the education program for systematical dental information should be delivered to their parents.

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Studies on the Development of Blood Sausage from By-products -Appreciation of Blood Sausage Qualities from the Animal Experiments- (부산물(副産物)을 이용(利用)한 혈액(血液)소시지 개발(開發)에 관한 연구(硏究) -동물실험(動物實驗)을 통한 제품가치(製品價値) 평가(評價)-)

  • Koh, Jin-Bog;Moon, Yoon-Hee;Kim, Jae-Young;Moon, Yeong-Duck
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.13 no.3
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    • pp.319-325
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    • 1984
  • This experiment aims to observe the nutritive value of blood sausages by manufacturing three kinds of them in order to utilize various by-products of swine when it is processed. In attempt to do so, five different diets were made: one control diet and four diets in which three kinds of blood sausages(blood sausage-1 was made with sausage meat 10%, fat 40%, blood 20%, skin 20% and liver 2%. blood sausage-2 was made with ham meat 30%, fat 20%, blood 20%, liver 2% and skin 20%. blood sausage-3 was made with smoked ham patch 30%, fat 20%, blood 20%, liver 2% and skin 20%) and mixed sausage(sausage meat 20%, fat 10% and fish meat 35%) were mixed with control diet at the rates of 10% respectively. These diets were fed to growing male and female albino rats for 6 weeks. The results obtained through this study are summarized as follows. In body weight gain of male rats, blood sausage-1 and 2 groups were significantly higher than mixed sausage group during the experimental period, and in that of female rats, control group and blood sausage-1 group were similar, but the other groups were lower than control group. Food intake was different in each group every week, but the amount of that was similar to each one throughout all experimental period. Food efficiency ratio and protein efficiency ratio in male were similar in each group, but slightly different in female during the feeding period. Liver weights of male in mixed sausage group and blood sausage-3 group were lower than those in control group and blood sausage-2 group, but liver weight of female in blood sausage-2 group was lower than that in control group. And all groups were similar in liver weight per 100 g of body weight. Mixed sausage group and blood sausage-1 group were lower than control group in kidney weight of male, and blood sausage-1 group was lower than control group in kidney weight of female. Kidney weight per 100 g of body weight of male showed that blood sausage-1 group was lower than the other groups. Spleen weight was similar in all groups. Hematocrit and hemoglobin contents in blood, total protein, albumin, albumin/globulin ratio, total lipid and total cholesterol contents in serum were showed almost similar in all groups.

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