• Food Science and Biotechnology
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• 제14권6호
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• pp.860-865
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• 2005

A gene for a putative glucoamylase, stg, of a hyperthermophilic archae on Sulfolobus tokodaii was cloned and expressed in Escherichia coli. The recombinant glucoamylase (STGA) had an optimal temperature of $80^{\circ}C$ and was extremely thermostable with a D-value of 17 hr. The pH optimum of the enzyme was 4.5. Being different from fungal glucoamylases, STGA hydrolyzed maltotriose (G3) most efficiently. Gel permeation chromatography and sedimentation equilibrium analytical ultracentrifugation analysis showed that the enzyme existed as a dimer. STGA was stable enough to hydrolyze liquefied com starch to glucose in 4 hr at $90^{\circ}C$ with a yield of95%. Comparison of the $k_{cat}$ values for the hydrolysis and the reverse reaction at $75^{\circ}C$ and $90^{\circ}C$ indicated that glucose production by STGA was more efficient at $90^{\circ}C$ than $75^{\circ}C$. Therefore, STGA showed great potential for application to the industrial glucose production process due to its high thermostability.

• Biomolecules & Therapeutics
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• 제20권2호
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• pp.220-225
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• 2012

To develop a ginseng product possessing an efficacy for diabetes, ginseng radix ethanol extract was treated with pectinase and obtained the GINST. In the present study, we evaluate the beneficial effect of GINST on high fat diet (HFD)-induced hyperglycemia and hyperlipidemia and action mechanism(s) in ICR mice. The mice were randomly divided into five groups: regular diet group (RD), high fat diet group (HFD), HFD plus GINST at 75 mg/kg (GINST75), 150 mg/kg (GINST150), and 300 mg/kg (GINST300). Oral glucose tolerance test reveals that GINST improves the glucose tolerance after glucose challenge. Fasting plasma glucose and insulin levels were decreased by 4.3% and 4.2% in GINST75, 10.9% and 20.0% in GINST150, and 19.6% and 20.9% in GINST300 compared to those in HFD control group. Insulin resistance indices were also markedly decreased by 8.2% in GINST75, 28.7% in GINST150, and 36.4% in GINST300, compared to the HFD control group. Plasma triglyceride, total cholesterol and non-esterified fatty acid levels in the GINST300 group were decreased by 13.5%, 22.7% and 24.1%, respectively, compared to those in HFD control group. Enlarged adipocytes of HFD control group were markedly decreased in GINST-treated groups, and shrunken islets of HFD control mice were brought back to near normal shape in GINST300 group. Furthermore, GINST enhanced phosphorylation of AMP-activated protein kinase (AMPK) and glucose transporter 4 (GLUT4). In summary, GINST prevents HFD-induced hyperglycemia and hyperlipidemia through reducing insulin resistance via activating AMPK-GLUT4 pathways, and could be a potential therapeutic agent for type 2 diabetes.

• Mycobiology
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• 제45권3호
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• pp.129-138
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• 2017

In this study, we aimed to characterize fungal samples from necrotic lesions on collar regions observed in different sweetpotato growing regions during 2015 and 2016 in Korea. Sclerotia appeared on the root zone soil surface, and white dense mycelia were observed. At the later stages of infection, mother roots quickly rotted, and large areas of the plants were destroyed. The disease occurrence was monitored at 45 and 84 farms, and 11.8% and 6.8% of the land areas were found to be infected in 2015 and 2016, respectively. Fungi were isolated from disease samples, and 36 strains were preserved. Based on the cultural and morphological characteristics of colonies, the isolates resembled the reference strain of Sclerotium rolfsii. Representative strains were identified as S. rolfsii (teleomorph: Athelia rolfsii) based on phylogenetic analysis of the internal transcribed spacer and large subunit genes along with morphological observations. To test the pathogenicity, sweetpotato storage roots were inoculated with different S. rolfsii strains. 'Yulmi' variety displayed the highest disease incidence, whereas 'Pungwonmi' resulted in the least. These findings suggested that morphological characteristics and molecular phylogenetic analysis were useful for identification of S. rolfsii.

• Journal of Ginseng Research
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• 제32권3호
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• pp.187-193
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• 2008

The present study was designed to investigate the anti-diabetic effect and mechanism of Korean red ginseng in C57BL/KsJ db/db mice. The db/db mice were divided into three groups: diabetic control group (DC), Korean red ginseng group (KRG, 100 mg/kg) and metformin group (MET, 300 mg/kg), and treated with drugs once per day for 10 weeks. Compared to the DC group, fasting blood glucose levels were decreased by 19.8% in KRG-, 67.7% in MET-treated group. With decreased plasma glucose and insulin levels, the insulin resistance index of the KRG-treated group was reduced by 27.6% compared to the DC group. The HbA1c levels in KRG and MET-treated groups were also decreased by 11.0% and 18.9% compared to that of DC group, respectively. Plasma triglyceride and non-esterified fatty acid levels were decreased by 18.8% and 16.8%, respectively, and plasma adiponectin and leptin levels were increased by 20.6% and 12.1%, respectively, in the KRG-treated group compared to those in DC group. Histological analyses of the liver and fat tissue of mice treated with KRG revealed significantly decreased number of lipid droplets and decreased size of adipocytes compared to the DC group. From the pancreatic islet double-immunofluorescence staining, we observed KRG has increased insulin contents, but decreased glucagon production. To elucidate action mechanism of KRG, effects on AMP-activated protein kinase (AMPK) and its downstream target proteins responsible for fatty acid oxidation and gluconeogenesis were explored in the liver. KRG activated AMPK and acetyl-coA carboxylase (ACC) phosphorylations, resulting in stimulation of fatty acid oxidation. KRG also caused to down regulation of SREBP1a and its target gene expressions such as FAS, SCD1 and GPAT. In summary, our results suggest that KRG exerted the anti-diabetic effect through AMPK activation in the liver of db/db mice.

• 한국의사학회지
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• 제16권2호
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• pp.105-140
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• 2003

1. "Eonhaegukupbang(諺解救急方)" is a medical book that was published and edited by Naeuiwon(內醫院) under the king's command in the Joseon Dynasty in 1607. 2. It includes the contents but doesn't a preface and an epilogue. And several medical treatments and herb medicines of diseases from emergency are noted in the original text of "Eonhaegukupbang(諺解救急方)". The book is composed of two volume and 181 pages. 3. A number of cultural properties and medical books were lost and all people of the nation were suffered from poverty and diseases during Japanese invasion of Korea in 1592. Then some books of Korean translation of Chinese classics were edited under the king's command with the intention of recompiling the lost books and easy application of medical knowledge for the people. 4. "Eonhaegukupbang(諺解救急方)" admit several books and notes the reference books at the beginning or the end. This book has 37 kinds of reference books, and those are "Boncho(本草)", "Uihakgangmok(醫學綱目)", "Uihakimmun(醫學入門)", "Deukhyobang(得效方)", "Uihakjeongjeon(醫學正傳)", "Dan-gyebang(丹溪方)" and so on in the main. 5. "Gugupbang(救急方)"(in the Sejo Dynasty) is similar to "Eonhaegukupbang(諺解救急方)" in aspect of helping the people with convenient contents. But it is a point of difference that the composition and contents of "Eonhaegukupbang(諺解救急方)" are more centering about the medical prescriptions than those of "Gukupbang(救急方)". 6. "Eonhaegukupbang(諺解救急方)" is very similar to the "Gukupmun救急門" in the "Donguibogam(東醫寶鑑)" in the contents and construction of the list. "Eonhaegukupbang(諺解救急方)" was published during the writing of "Donguibogam(東醫寶鑑)", so it has a deep meaning that "Eonhaegukupbang(諺解救急方)" is a condensed version of easy application for the civilian by regulating already established section. 7. It seems that "Eonhaegukupbang(諺解救急方)" has continuous influences in the part of emergency in the "Donguibogam(東醫寶鑑)"

• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제44회 동계 정기학술대회 초록집
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• pp.144-144
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• 2013

Non-thermal atmospheric pressure plasmas have recently garnered much attention due to their unique physical and chemical properties that are sometimes significantly different from those of low pressure plasmas. It can offer many possible application areas including nano and bio/medical areas. Many different types of plasma sources have been developed for specific needs, which can be one of the important merits of the atmospheric pressure plasmas since characteristics of the produced plasma depend significantly on operating parameters such as driving frequency, supply gas type, driving voltage waveform, gas flow rate, gas composition, geometrical factor etc. Among many source configurations, parallel plate type geometry is one of the simplest configurations so that it can offer many insights for understanding basic underlying physics. Traditionally, the parallel plate type set up has been studied actively for understanding low pressure plasma physics along with extensive employment in industries for the same reason. By considering that understanding basic physics, in conjunction with plasma-surface interactions especially for nano & bio materials, should be pursued in parallel with applications, we investigated atmospheric pressure discharge characteristics in a parallel plate type capacitive discharge source with two parallel copper electrodes of 60 mm in diameter and several millimeters in gap distance. In this presentation, some plasma characteristics by varying many operating variables such as inter-electrode distance, gas pressure, gas composition, driving frequency etc will be discussed. The results may be utilized for plasma control for widening application flexibility.

• 한국인터넷방송통신학회논문지
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• 제13권5호
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• pp.1-8
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• 2013

본 논문에서는, 차량 섀시 제어시스템 중 ACC(Adaptive Cruise Control)와 LKS(Lane Keeping System)를 통합하여 차량용 OS인 OSEK OS 기반으로 구현하고 실험을 통하여 그 성능을 분석한다. 최근 자동차의 안전성을 향상시키기 위해 능동 안전 및 운전자 지원 시스템이 논의 되고 있다. 그 중 차량의 종방향에 대한 가감속 제어를 수행하는 ACC와 차량이 차선을 유지할 수 있도록 보조해주는 LKS를 통합하여 차량 내 제어시스템을 구현한다. 구현한 제어시스템은 차량용 소프트웨어의 재사용성과 안전성 및 응용 소프트웨어의 하드웨어 의존성 제거 등을 목표로 표준 제안된 OSEK/VDX를 적용한다. OSEK/VDX에서 지원하는 OSEK OS를 기반으로 재설계한 제어시스템은 실시간 태스크 관리와 인터럽트 처리 및 공유자원 관리가 가능하다. EILS(ECU-In-the-Loop Simulation) 수행 결과에 의해 OSEK OS 기반의 ACC/LKS 통합제어기와 기존 일체형 펌웨어로 구현한 ACC/LKS 통합제어기가 대등함을 입증한다.

• 한국병원경영학회지
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• 제25권1호
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• pp.46-57
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• 2020

Objectives: The purpose of this study is to evaluate the factors affecting patient satisfaction for the nursing service of comprehensive nursing care service unit in Tertiary hospital Methods: This study employed a descriptive survey to investigate the nursing satisfaction and the study data was collected from 74 patients among 83 patient who were admitted to the nursing care integration service ward of an tertiary hospital for 10 days from May 9, 2018 to May 19, 2018. Data was analyzed by descriptive statistics, t-test, ANOVA, and multiple linear regression using SPSS 21 program. Results: The average with nursing satisfaction was significantly different according to marriage type and KPCS level. It was found that physical factor and educational factor have statistically significant effect on nursing satisfaction. As physical care increase by one unit, patient satisfaction increase by 0.226(95% CI; 0.061-0.390) and also satisfaction increase by 0.443(95% CI; 0.070-0.816) as education care increase by one unit. Conclusions: As a result of study, it was found that it is necessary to provide patients with more careful nursing service in physical and educational perspective, in order to improve the service quality of comprehensive nursing care service unit.

• 설비공학논문집
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• 제26권12호
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• pp.579-587
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• 2014

This paper deals with precise analytical state equation modeling of a variable speed refrigeration system (VSRS) for optimum control in state space. The VSRS is described as multi-input and multi-output (MIMO) system, which has two controlled variables and two control inputs. First, the Navier-Stokes equation and mass flow rate were applied to each component of the basic refrigeration cycle to build a dynamic model. The dynamic model, represented by a differential equation, was transformed into the state equation formula. Next, a full-order state observer was built to estimate all of the state variables to compose an optimum control system. Then, an optimum controller was designed to minimize an evaluation function that has input energy and control error. Finally, simulations and experiments were conducted to verify the validity of the proposed modeling and designed optimum controller to regulate target temperature and superheat in a 1RT oil cooler system. The results show that the proposed method, state equation modeling and optimum control, is efficient to ensure optimal control performance of the VSRS.

• 한국컴퓨터정보학회논문지
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• 제12권5호
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• pp.29-37
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• 2007

본 논문에서는 일반적인 지진 데이터 전송방식보다 좀 더 빠르게 수집될 수 있는 MMA 데이터를 이용하는 새로운 실시간 지진동 감시 시스템을 제안한다. 제안 시스템은 관측소로부터 전송되는 20sps 데이터 기반의 초당 최대, 최소 및 평균값을 연속적으로 수신한다. 그리고 지진동의 크기로써 PGA를 계산하고 이를 가시화하여 우리나라의 지진동을 감시한다. MMA 데이터로부터 계산된 PGA 데이터를 검증하기 위하여 2002년 6월 29일 중국 무단장에서 발생한 지진 데이터를 분석하였으며, 2007년 1월 20일 강원도 오대산에서 발생한 지진 데이터의 로그 파일을 사용하여 제안한 시스템을 정밀히 검증하였다. 실험결과로서 제안한 시스템은 기존 방법보다 빠르게 이벤트(지진)를 감지하며, 유용한 정량적 정보를 생성하는 것으로 판명되었다.