• 대한한의학회지
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• 제27권1호
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• pp.184-195
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• 2006

Objectives : Betula Platyphylla(BP) is a traditional analgesic, anti-fungal, anti-inflammatory herb used in Chinese 1medicine. However, no information is available to explain its action. In this study. we investigated the anti-inflammatory 1effects of BP to elutidate the molecular pharmacological activity in the ethanol extract of BP(BPE). Methods : We performed WTS assay in lipopolysaccharide (LPS) stimulated RAW264.7 macrophages with BPE. Nitrite was measured by Griess assay, prostaglandin E2 (PGE2) by enzyme-linked immunosorbent assay (ELISA) in LPS induced RAW264.7 macrophages with BPE. Inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX-2) were determined by Western blot. Activation of nuclear factor-kappaB (NF-kB) was measured by electrophoretic mobility shift assay (EMSA). Results : BPE significantly suppressed production of nitric oxide (NO) and PGE2 in LPS-induced RAW264.7 macrophages in a dose-dependent manner. The maximal inhibition rate of NO and PGE2 production by BPE was ca. 88.8% and 93% at the concentration of $100{\mu}g/ml$ (non-cytotoxic concentration), respectively. BPE also decreased iNOS protein and COX-2 protein in LPS-induced RAW264.7 macrophages. EMSA demonstrated that BPE inhibited the DNA binding activity of the NF-kB. Conclusions : These results suggest that BPE inhibits NF-${\kappa}B$-mediated gene expression and downregulates inflammatory mediator production in RAW264.7 macrophages.

• 한국강구조학회 논문집
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• 제24권6호
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• pp.613-625
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• 2012

철도교는 일반적으로 설계하중에 근접한 열차하중이 빈번하게 통과할 가능성이 있으므로, 초기설계단계부터 여러 구조상세에 대해 피로특성을 충분한 반영하는 것이 필요하다. 그럼에도 불구하고 철도교에 작용하는 축하중의 크기 및 배치 특성, 피로에 대한 부적절한 구조상세의 적용 등으로 인하여 강철도 플레이트거더교에서 부분적으로 피로균열이 보고되고 있다. 상부플랜지와 복부의 용접이음부에서의 피로균열의 주요 발생 원인의 하나는 침목을 지지하는 주거더와 열차하중이 작용되는 레일과의 중심간격 차이에 따른 열차하중의 편심작용에 의한 것이다. 이 연구에서는 공용중인 강철도 상로플레이트거더교를 대상으로 현장조사 및 현장계측, 일련의 구조해석을 실시하고, 대상교량의 구조거동 특성 및 피로균열의 발생 원인의 규명, 보수 보강(안)에 대하여 검토하였다.

• 인간행동과 음악연구
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• 제17권1호
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• pp.97-125
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• 2020

본 연구는 자폐범주성장애(ASD) 아동의 또래 간 상호작용 촉진을 위한 동기화 기반 그룹 음악치료 사례연구이다. 중재 프로그램은 ASD 아동의 사회기술 발달에 있어 운동조절이 미치는 요인을 확인한 선행연구 결과를 반영하여 구성되었다. 총 5명의 ASD 아동이 대상자로 참여하였으며, 중재는 주 2회씩 총 8회기로 진행되었다. 결과분석을 위해 중재 안에서 관찰된 공동 행동 참여, 동시적 움직임, 눈맞춤 시도, 상호작용 시도 행동의 발생률과 중재 과정에서 나타난 의미 있는 음악행동 변화를 분석하였으며, 세션 내 관찰행동의 변화가 외부 환경에서도 유사하게 관찰되는지 확인하기 위하여 대상자의 부모에게 사전·사후 부모용 또래 놀이행동 척도(PIPPS-P) 검사를 실시하였다. 분석 결과, 중재에 참여한 대상자 모두 또래 간 동기화된 움직임 및 또래에 대한 자발적인 상호작용 시도 행동, 또래 놀이행동 척도 점수가 증가했음을 알 수 있었다. 이러한 결과는 ASD 아동의 지각-운동 연합에 있어서의 문제를 반영한 동기화 기반 그룹 음악치료가 ASD 아동 간 동기화된 움직임을 유도하는 데 효과적임을 보여주는 것이다. 본 연구는 동기화에 기반을 둔 음악치료적 개입이 어떻게 ASD 아동의 또래 상호작용을 촉진할 수 있는지 임상 현장에서 구체적으로 적용 가능한 기초자료를 제공하였다는 점에서 그 의의가 있다.

• 동의생리병리학회지
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• 제29권4호
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• pp.305-312
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• 2015

Paeonol is a major component found in the Paeoniaceae family such as Paeonia suffruticosa Andrews. Paeonia suffruticosa Andrews has traditionally been used to enhance blood flow and relieve joint pain in east Asian countries including China, Korea and Japan. Current research has shown that paeonol blocked the voltage-gated sodium channel and L-type calcium channel. However, there is a lack of research to reveal the relation between cardiac function and blockade of ion channels by paeonol. Therefore, the aim of this study is to investigate whether paeonol has anti-arrhythmic effects via modulating cardiac ion channels. It is collected that the effects of paeonol on multiple ion channels such as the fast sodium channel and L-type calcium channel from published papers. To incorporate the information on multi-channel block, we computed the effects using the mathematical cardiac model of the guinea-pig and rat ventricular cells (Noble 1998 and 1991 model) and induced early after-depolarizations (EADs) to generate an arrhythmia in the whole heart. Paeonol slightly shortened the action potential duration in the normal cardiac ventricular action potential by the inhibition of sodium channel and L-type calcium channel. Paeonol presented the protective effect from EADs by the inactivation of sodium channel but not L-type calcium channel. Paeonol did not show any changes when it treated on normal ventricular cells through the inhibition of sodium channel, but the protective effect of paeonol through sodium channel on EADs was dose-dependent. These findings suggest that paeonol and its original plant may possess anti-arrhythmic activity, which implies their cardioprotective effects.

• 대한물리치료과학회지
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• 제5권4호
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• pp.785-794
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• 1998

We have made a survey of 40 patients in the university hospitals and oriental medical centers in Seoul from Sep. 1, 1997 to Mar. 1, 1998. We sampled 25 of them and the result shows that there were 12 MCA damaged patients(48%), 5 SAH(20%), 5 ACA(20%), 2 PCA (8%), 1 PCOA(4%). The number of MCA patients were the most. 1. As the cause of each disease, 4 of the 12 MCA damaged patients(33.35%) have infarction and cerebral hemorrhage, 2 of 5 SAH patients(40%) have cerebral hemorrhage and head injury, 3 ACA damaged patients have cerebral hemorrhage. 11 of 25 brain bloodvessel damaged patients(44%) were hemorrhage patients. 2. Rt. hemiparesis was the main symptom of 6 of 12 MCA damaged patients(50%) and 3 of 5 SAH patients(60%), and the main symptom of 3 of 5 ACA patients(60%) was Lt. hemiparesis. The main symptom of 13 of 25 brain bloodvessel damaged patients(52%) was Lt. hemiparesis 11 of them(44%) Rt. hemiparesis, and 1 of them(8.3%) Quadriplegia. 3. Language was the most well preserved function. 12 MCA damaged patients could understand language. 4. Retraction of shoulder girdle, among VIE flexor synergy, was the most frequent element because 9 of 12 MCA damaged patients had it. Among VIE flexor synergy, 5 SAH patient's most frequent synergy was Elbow flexion because all of them had it. All of 5 ACA damaged patients have shoulder girdle elevation, shoulder joint, hyperextension, abduction, and external rotation among VIE flexor synergy. 5. 7 of 12 MCA damaged patients(58.3%) were stereognosis handicapped patients, 3 of 5 SAH patients(60%) have handicap of position sense, light touch, and temperature, 3 of 5 ACA patients(60%) have position handicap. 13 of brain bloodvessel damaged patients(52%) have light touch handicap. 6. 8 of MCA damaged patients(66.7%) have facial palsy, 4 of SAH damaged patients(80%) have memory and action decline, and 3 of ACA damaged patients(60%) have action decline and facial palsy. The problem of Hemiplegia is very extensive from muscle weakness, atrophy, or deformation to psychical problems. Therefore physical therapists should have sufficient interest in psychological handicap as well as physical handicap as they deal with adult hemiplegia.

• Molecules and Cells
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• 제37권8호
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• pp.620-627
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• 2014

We have previously shown that microRNAs (miRNAs) miR-760, miR-186, miR-337-3p, and miR-216b stimulate premature senescence through protein kinase CK2 (CK2) downregulation in human colon cancer cells. Here, we examined whether these four miRNAs are involved in the replicative senescence of human lung fibroblast IMR-90 cells. miR-760 and miR-186 were significantly upregulated in replicatively senescent IMR-90 cells, and their joint action with both miR-337-3p and miR-216b was necessary for efficient downregulation of the ${\alpha}$ subunit of CK2 ($CK2{\alpha}$) in IMR-90 cells. A mutation in any of the four miRNA-binding sequences within the $CK2{\alpha}3^{\prime}$-untranslated region (UTR) indicated that all four miRNAs should simultaneously bind to the target sites for $CK2{\alpha}$ downregulation. The four miRNAs increased senescence-associated ${\beta}$-galactosidase (SA-${\beta}$-gal) staining, p53 and $p21^{Cip1/WAF1}$ expression, and reactive oxygen species (ROS) production in proliferating IMR-90 cells. $CK2{\alpha}$ overexpression almost abolished this event. Taken together, the present results suggest that the upregulation of miR-760 and miR-186 is associated with replicative senescence in human lung fibroblast cells, and their cooperative action with miR-337-3p and miR-216b may induce replicative senescence through $CK2{\alpha}$ downregulation-dependent ROS generation.

• 한국산학기술학회논문지
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• 제17권4호
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• pp.400-413
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• 2016

80 MPa급 고강도 콘크리트가 강거더의 압축 플랜지로 대체하는 아중합성 거더의 경우, 플랜지와 케이싱 및 케이싱과 바닥판 2개의 접합부 계면이 형성되는데 각 계면의 수평 전단 저항능력은 구조물의 안전성에 있어 중요한 요소이다. 본 연구는 계면 상세를 달리한 6개의 실험체를 도로교설계기준(한계상태설계법)에 따라 설계 및 제작하여 이중합성 보의 휨 파괴 대비 수평 전단에 대한 구조 성능 실험을 수행하였다. 실험체의 주요변수로 스터드 전단연결재의 저항계수, 바닥판 콘크리트 및 철근의 재료저항계수, 콘크리트 인장강도에 따른 부착계수, 케이싱 콘크리트의 표면 상태 그리고 수평전단철근의 간격을 고려하였다. 실험 결과, 강재 상부 플랜지와 고강도 케이싱의 계면이 고강도 케이싱과 바닥판 계면 보다 결합성이 큰 것으로 나타났다. 그리고 고강도 케이싱과 바닥판 계면에서는 케이싱 표면에 요철 또는 거칠기를 주는 것 보다 보수적으로 수평전단철근을 배근하는 것이 소성영역까지 합성 거동을 유지하는 것으로 나타났다.

• Geomechanics and Engineering
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• 제36권3호
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• pp.303-316
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• 2024

Seismic records are composed of mainshock and a series of aftershocks which often result in the incremental damage to underground structures and bring great challenges to the rescue of post-disaster and the repair of post-earthquake. In this paper, the repetition method was used to construct the mainshock-aftershocks sequence which was used as the input ground motion for the analysis of dynamic time history. Based on the Daikai station, the two-dimensional finite element model of soil-station was established to explore the failure process of station under different seismic precautionary intensities, and the concept of incremental damage of station was introduced to quantitatively analyze the damage condition of structure under the action of mainshock and two aftershocks. An arc rubber bearing was proposed for the shock absorption. With the arc rubber bearing, the mode of the traditional column end connection was changed from "fixed connection" to "hinged joint", and the ductility of the structure was significantly improved. The results show that the damage condition of the subway station is closely related to the magnitude of the mainshock. When the magnitude of the mainshock is low, the incremental damage to the structure caused by the subsequent aftershocks is little. When the magnitude of the mainshock is high, the subsequent aftershocks will cause serious incremental damage to the structure, and may even lead to the collapse of the station. The arc rubber bearing can reduce the damage to the station. The results can offer a reference for the seismic design of subway stations under the action of mainshock-aftershocks.

• 근관절건강학회지
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• 제25권1호
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• pp.21-29
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• 2018

Purpose: The purpose of this study was to investigate the effects of a community-based empowerment program for resident leaders participating in the Healthy Apartment project. Methods: The design of this study was a single group pre-post test design. This program was consisted of 4-hour sessions. The total participants were 22 leaders. Results: The participants showed significant changes in social support (Z=-2.33, p=.020), collective action (Z=-3.91, p<.001), conflict and homogeneity (Z=-2.03, p=.042), and community perception index (Z=-3.97, p<.001) as sub factors of the individual level of SC-IQ (Integrated Questionnaire for the Measurement of Social Capital). They also showed significant changes in necessity of the resident organization (Z=-4.13, p<.001), resident participation (Z=-3.84, p<.001), convergence of resident opinion (Z=-3.94, p<.001) and the role of community leaders (Z=-3.94, p<.001) as sub factors of the perception and confidence of resident leaders (Z=-3.85, p<.001). Conclusion: Results indicate that the community-based empowerment program is effective in perceptions of social capital on the individual level and improvement of recognition as resident leaders and confidence for resident leaders.

• Structural Engineering and Mechanics
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• 제74권4호
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• pp.457-470
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• 2020

The force resisting ability of a connection has direct implications on the overall response of a timber framed structure to various actions, thereby governing the integrity and safety of such constructions. The behavior of timber framed structures has been studied by many researchers by testing full-scale-connections in timber frames so as to establish consistent design provisions on the same. However, much emphasis in this approach has been unidirectional, that has focused on a particular connection configuration, with no research output stressing on the refinement of the existing connection details in order to optimize their performance. In this regard, addition of adhesive to dowelled timber connections is an economically effective technique that has a potential to improve their performance. Therefore, a comparative study to evaluate the performance of various full-scale timber frame Nailed connections (Bridled Tenon, Cross Halved, Dovetail Halved and Mortise Tenon) supplemented by adhesive with respect to Nailed-Only counterparts under tensile loading has been investigated in this paper. The load-deformation values measured have been used to calculate stiffness, load capacity and ductility in both the connection forms (with and without adhesion) which in turn have been compared to other configurations along with the observed failure modes. The observed load capacity of the tested models has also been compared to the design strengths predicted by National Design Specifications (NDS-2018) for timber construction. Additionally, the experimental behavior was validated by developing non-linear finite element models in ABAQUS. All the results showed incorporation of adhesive to be an efficient and an economical technique in significantly enhancing the performance of various timber nailed connections under tensile action. Thus, this research is novel in a sense that it not only explores the tensile behavior of different nailed joint configurations common in timber construction but also stresses on improvising the same in a logical manner hence making it distinctive in its approach.