대한한의학회지 (The Journal of Korean Medicine)
- 제27권1호
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- Pages.184-195
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- 2006
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- 1010-0695(pISSN)
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- 2288-3339(eISSN)
화피(樺皮)의 항염(抗炎) 효과(效果)에 관(關)한 연구(硏究)
Anti-inflammatory Effects of an Ethanolic Extract form Betula Platyphylla
- 유미현 (경희대학교 동서 골관절연구원) ;
- 박은경 (경희대학교 동서 골관절연구원) ;
- 김영훈 (경희대학교 동서 골관절연구원) ;
- 이연아 (경희대학교 의과대학 내과학교실) ;
- 이상훈 (경희대학교 의과대학 내과학교실) ;
- 양형인 (경희대학교 의과대학 내과학교실) ;
- 홍승재 (포천중문의과대학 류마티스내과학교실) ;
- 백용현 (경희대학교 한의과대학 침구학교실) ;
- 박동석 (경희대학교 한의과대학 침구학교실) ;
- 한정수 (경희대학교 의과대학 정형외과학교실) ;
- 유명철 (경희대학교 의과대학 정형외과학교실) ;
- 김경수 (경희대학교 동서 골관절연구원)
- Ryu Mi-Hyun (East-West Bone & Joint Research Center, College of Medicine, Kyung Hee University) ;
- Park Eun-Kyung (East-West Bone & Joint Research Center, College of Medicine, Kyung Hee University) ;
- Kim Young-Hoon (East-West Bone & Joint Research Center, College of Medicine, Kyung Hee University) ;
- Lee Yeon-Ah (Dept. of Internal Medicine, Kyung Hee University) ;
- Lee Sang-Hoon (Dept. of Internal Medicine, Kyung Hee University) ;
- Yang Hyung-In (Dept. of Internal Medicine, Kyung Hee University) ;
- Hong Seung-Jae (Dept. of Internal Medicine, College of Medicine, Pochon CHA University) ;
- Baek Yong-Hyeon (Dept. of College of Medicine, Kyung Hee University) ;
- Park Dong-Suk (Dept. of College of Medicine, Kyung Hee University) ;
- Han Jung-Soo (Dept. of Orthopedic Surgery, College of Medicine, Kyung Hee University) ;
- Yoo Myung-Chul (Dept. of Internal Medicine, College of Medicine, Kyung Hee University) ;
- Kim Kyoung-Soo (East-West Bone & Joint Research Center, College of Medicine, Kyung Hee University)
- 발행 : 2006.03.01
초록
Objectives : Betula Platyphylla(BP) is a traditional analgesic, anti-fungal, anti-inflammatory herb used in Chinese 1medicine. However, no information is available to explain its action. In this study. we investigated the anti-inflammatory 1effects of BP to elutidate the molecular pharmacological activity in the ethanol extract of BP(BPE). Methods : We performed WTS assay in lipopolysaccharide (LPS) stimulated RAW264.7 macrophages with BPE. Nitrite was measured by Griess assay, prostaglandin E2 (PGE2) by enzyme-linked immunosorbent assay (ELISA) in LPS induced RAW264.7 macrophages with BPE. Inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX-2) were determined by Western blot. Activation of nuclear factor-kappaB (NF-kB) was measured by electrophoretic mobility shift assay (EMSA). Results : BPE significantly suppressed production of nitric oxide (NO) and PGE2 in LPS-induced RAW264.7 macrophages in a dose-dependent manner. The maximal inhibition rate of NO and PGE2 production by BPE was ca. 88.8% and 93% at the concentration of