• Journal of The Korean Society of Grassland and Forage Science
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• v.14 no.2
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• pp.82-87
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• 1994

This study was planned to identify the variety of Italian ryegrass using electrophoresis. Thirty seven varieties of Italian ryegrass were tested by starch gel electrophoresis. The specific electrophoretic zymograms of each variety were observed by Alcohol dehydrogenase(ADH) and Esterase. The results were surnrnerized as follows; 1. AU varieties displayed two band zones by ADH and Rf values were 0.63 and 0.6 (Table 2, Fig. 2). 2. There were five band type for ADH isozyme of 37 varieties classified with isozyme banding pattern. According to the isozyme band type 7, 2, 6, 18 and 4 varieties belong to group, I, II, III, IV, and V, respectively (Table 2). 3. The varieties displayed single band zone for Esterase isozyme and Rf value was 1.00 (Table 2, Fig. 4). 4. According to banding type, Esterase isozyme of 37 varieties classified into 3 groups, 22, 8 and 7 varieties belong to group, I , II, and III, respectively (Table 2).

• The Korean Journal of Zoology
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• v.31 no.3
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• pp.225-235
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• 1988

This study was carried out to compare distribution and isozyme pattern of nonspecific esterase, acid phosphatase and alkaline phosphatase on developing sparganum and adult of Spinometra erinacei by using enzyme-histochemical method and electrophoresis The sparganum and adult were recovered from rats and cat that were infected by sparganum. The results obtained were as follows: Nonspecific esterase had a strong activity in the parenchymal musculature of sparganum and adult, but no detectable level in ihe tegument. A total of 7 and 8 nonspecific esterase bands were detectable in sparganum and adult, respectively. Of these bands, band 3 and 4 were major bands in sparganum and adult. Acid phosphatase had a strong activity in the tegument and the epidermal musculature of sparganum, but no detectable level in the parenchymal musculature. A total of 3 bands were detectable in sparganum and adult. Of these bands, band 3 was major band in sparganum and adult. Alkaline phosphatase had a strong activity in the tegument and the epidennal musculature of sparganum and of adult, but no detectable level in the parenchymal musculature. A total of 2 and 4 bands were detectable in sparganum and adult. Of these bands, band 2 was major band in sparganum and adult. Based on the present results isozyme band patterns showed qualitative and quantitative changes in each tissues of sparganum and of adult during the development.

• The Korean Journal of Mycology
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• v.16 no.2
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• pp.87-94
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• 1988

Electrophoretic isozyme patterns from mycelia, primordia, cap and stem of Pleurotus spp. collected in Korea were compared. Primordia, cap and stem of fruitbody showed very similar isozyme patterns but mycelial isozyme patterns were different from those of fruitbody. Isozyme patterns of malate dehydrogenase, acid phosphatase in Pleurotus ostreatus collected from different regions in Korea were similar but those of esterase, peroxidase, leucine amino peptidase and superoxide dismutase were different. Interspecific comparison of esterase isozyme patterns among Pleurotus ostreatus P. cornucopiae and, P. florida was very different and may be valuable subsidiary tool to conventional taxonomic techniques for identifying species of Pleurotus. A dendrogram by similarities of isozyme band pattern was presented.

• The Korean Journal of Zoology
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• v.22 no.1
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• pp.1-10
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• 1979

From the experimental results of cellulose acetate membrane electrophoresis we concluded the followings in explaining the LDH isozyme patterns found in the retina and cerebrum of vertebrata. Lactate dehydrogenase of the retina and cerebrum of both Carassinus carassinus and Cyprinus carpio was found to have one diffused band located between $LDH_2$ and $LDH_1$. LDH isozyme patterns of heart, pectoral muscle, liver and stomach of the Cyprinus carpio had the same diffused band in all organs. LDH isozyme patterns of the cerebrum of Hynobius leechii and Rana nigromaculata were observed to be different, in Hynobius leeichi a single band moved to the negative pole and two bands of $LDH_5$ and $LDH_4$ were obtained in the Rana nigromaculata. The retina and cerebrum of Natrix tigrina lateralis were observed as one band but amyda maakii had different LDH isozymes of the retina and cerebrum. The retina of Amyda maakii had five distinct LDH isozyme bands which had decreasing activity in the order of $LDH_5, LDH_4, LDH_3, LDH_2 and LDH_1$. The cerebrum of Amyda maakii had one band like Natrix tigrina lateralis but it moved to the negative pole. LDH isozymes in the retina and cerebrum of Gallus gallus domesticus and Melopsittacus undulatus showed one band. Five characteristic LDH isozyme bands were obtained from the retina of mammals, Oryctolagus cuniclus, Canis familiaris, Sus scrofa bos taurus and in the cerebrum of mouse, albino rat, Rhinolophus ferrum-equinum kokai.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.33 no.2
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• pp.122-125
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• 1988

A comparative electrophoretic study on protein and several important enzymes of calli induced from stem, intercostal area and minor vein area were conducted in Pinella ternata (Thunb.) Breit. Soluble protein band patterns of calli induced from stem, intercostal area and minor vein area were distinctly different from those of the corresponding plant parts. Esterase isozyme patterns of calli induced from stem, intercostal area and minor vein area were different from those of the corresponding plant parts. Glutamate oxalo-acetate transaminase isozyme patterns of calli grown for 4 weeks induced from 3 plant parts were similar to those of the corresponding plant parts. But a high molecular weight isozyme band appeared in the calli grown for 8 weeks. Alow molecular weight isozyme band disappeared on the peroxidase isozyme patterns of calli grown for 4 weeks appeared on those of the corresponding plant parts.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.2
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• pp.174-176
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• 1991

A electrophoretic study on seveal important enzymes was conducted with seed, leaf and stem of Benincasa hipida (Thunb) Cogn and Commelina communis L. Isozyme patterns of esterase were different from the parts and fruit of wax gourd appeared only one band. In the peroxidase isozyme, fruit of wax gourd appeared four bands, but stem of common dayflower no appeared. In the amylase isozyme, seed and fruit of 'gourd were appeared clearly one band, respectively.

• Journal of The Korean Society of Grassland and Forage Science
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• v.12 no.1
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• pp.71-76
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• 1992

Starch gel electrophoresis was used to examine the banding pattern of Esterase isozyme in the leaf, root-nodule and seedling of four wild legume species, Trifolim repense, Glycine soja, Phaseolus nipponensis and Vigna uexillata. The number of band, enzyme activity and migrating rate of esterase isozyme varies depending on the species and tissues of legume plants. The isozyme banding pattern in the cotyledon and radicle of T. repense showed same pattern, however, the number of band were varible among the cotyledon of G. soja, P. nipponensis and V. vexzllata, respectively. Est-1 in the leaf of G. soja, V, vexillata. root-nodule of G. soja and seedling of V. vexillata expreesed the highest enzyme activity. The Est-1 showed the rapidest migrating rate among the isozymes.

• Korean Journal of Environmental Biology
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• v.17 no.2
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• pp.209-215
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• 1999

Changes of malate dehydrogenase isozyme in oyster exposed to different temperature, pH and salinity were investigated by polyacrylamide gel electrophoresis. MDH isozyme in control group was separated into two bands on the positive side. In case of temperature and pH stress, MDH isozyme was separated into only one band after 12 hours exposure but two bands after 24, 48 hours exposure on the positive side. In case of salinity stress, after 12 hours exposure, MDH isozyme bands were separated into two bands in 5 ppt, 30 ppt and three bands in 10 ppt, 40 ppt concentration on the positive side. After 24 hours and 48 hours exposure case in salinity stress, MDH isozyme bands was separated into two bands on the positive side in all concentration. Activities of isozyme bands show their characteristics according to the condition of experiment. In conclusion, changes of MDH isozyme was a biochemical defense mechanism in oyster and result from effect of environmental stress to oyster.

• The Korean Journal of Zoology
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• v.15 no.3
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• pp.105-110
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• 1972

A cellulosse acetate electrophoretic survey of Korean Anura has revealed the presence of diverse lactate and malate dehydrogenase (LDH and MDH) isozymes. The pattern of LDH and MDH isozymes in the tissues of the central nervous system of the six species of Anura examined are species specific and differ from those of mammals and birds. Both Rana nigromaculata nigromaculata and Rana nigromaculata coreana have two molecular forms of LDH and MDH, respectively, with almost the same pattern. Whole brain homogenate of Rana temporaria shows also a maximum of only two LDH isozymes. Both Bufo bufo asiaticus and Bombina orientalis have five molecular forms of LDH with an entirely different spacing on the zymograms, whereas Rana rugosa has three. Two molecular forms of MDH are present in all animals examined and one band is shown in olfactory lobe and mixture of cerebellum and medulla oblongata of Rana nigromaculata nigromaculata.

• Journal of The Korean Society of Grassland and Forage Science
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• v.11 no.4
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• pp.199-202
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• 1991

The esterase isozyme of several legume plants were separated and visualized by horizontal starch gel electrophoresis using enzyme-specific staining. Extracts used were prepared from fully expanded young leaf and stem of six legume species which were red clover(Trifolium Pretense L.), ladino clover(Trifolium repense L.), wild white clover(Trifolium repense L.), alfalfa(Medicage sativa L.), mimosoides(Cassia mimosoides var nomame Makino), and amoena(Vicia amoena Fisch). The number of band, Phenotype and staining intensity of esterase isozyme in leaf and stem varies depending on the plant species. However, there are little difference between leaf and stem esterase isozyme in same species except alfalfa. And in the leaf and stem of mimosoides and amoena showed not any esterase(Fig. 2). Among the examined plants, the highest staining intensity and the rapidest migrating esterase isozyme was Est 1.