• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
• /
• v.4 no.4
• /
• pp.305-311
• /
• 1999

This study aims to decipher surface water mass interaction in summer in the South China Sea and East China Sea by radium isotope distribution pattern. Salinity and activity ratio of radium ($^{228}Ra/^{226}Ra$) showed gradual changes, which were adequate to apply simple two end-member mixing between Kuroshio surface water and Changjiang Dilute Water for the East China Sea and the former and Nearshore Diluted Watermass (NDW) for the South China Sea. Two tracer methods, salinity and Ra isotope ratio, were compared for East China Sea. Results showed remarkable consistency for waters near Kuroshio, however, discrepancy were noticeable after Tsushima Warm Current branching. Mixing with subsurface waters may cause the discrepancy. When mixed with subsurface waters, salts and radium isotope ratio are expected to be biased in opposite direction, i. e. prone to underestimate the fraction of less saline water in the case of salts and vice versa for Ra isotope ratio. Taking the mean values of two different results seems more realistic to estimate fraction of end-members.

• Journal of the Mineralogical Society of Korea
• /
• v.28 no.2
• /
• pp.187-193
• /
• 2015

The purpose of the present study is to trace the provenance of lead raw materials using the lead isotope ratio of 9 lead glasses excavated from the Sarira hole of Mireuksaji stone pagoda and to determine correlation between them and other lead glasses excavated from the Wanggungri site. The results of chemical analysis of the 9 lead glasses show that they are common lead glass system($PbO-SiO_2$) with respect to the contents of PbO (70 wt.%) and $SiO_2$ (30 wt.%). The lead isotope ratios of them plot to northern Korean peninsula when applied to the distribution map of lead isotopes of East Asia. On the other hand, southern Korean peninsula is verified as the main deposits of the lead ore in the distribution map of lead isotopes of South Korea. With respect to the results, it is notable that the provenance of the 9 lead glasses can be very different depending on the distribution map. In addition, a comparative study between them and the lead glasses excavated from the Wanggungri which was built in the same region and period shows that their lead isotopes are highly correlated.

• Conservation Science in Museum
• /
• v.9
• /
• pp.105-116
• /
• 2008

The Conservation Science Team of National Museum of Korea has established the data base of lead isotope ratio as the scientific research of bronze patina, which was acquired from conservtion process of metal objects, and based on this result, it intends to conduct the research related to the origin of raw material for the bronze objects. As the equipment for analysis of lead isotope ratio of the bronze patina, the thermal ionization mass spectrometer(TIMS) was used. As a part of this study, in 2nd year 2008, lead isotope ratios of total 18 samples inclding 2 samples of Round-type gold-bronze belong to Baekje period, the 2 items of head of bronze arrow belong to Nangnang(Lelang) and 10 items of the flower-shaped bronze dishes from the Unified Silla period, the 4 items of the bronze patina from the objects(era of 1 item not identified) of Wonpungtongbo(year 1078-North Sung) were analyzed.

• Journal of Conservation Science
• /
• v.32 no.2
• /
• pp.179-188
• /
• 2016

This paper presents a new information on animal husbandry practice in the Baekje period using stable isotope analysis of 17 animal bones excavated from the Pungnaptoseong Fortress. Stable carbon isotope evidence implies that wild animals such as deer and pigs had mainly $C_3$-based diet whereas domestic cattle and horses might have consumed more $C_4$ plants. In addition, we propose one potential that domestic pigs show higher nitrogen isotope values in comparison with wild boar because they were possibly fed by humans.

• Korean Journal of Environmental Agriculture
• /
• v.25 no.4
• /
• pp.371-381
• /
• 2006

New proteomic techniques have been pioneered extensively in recent years, enabling the high-throughput and systematic analyses of cellular proteins in combination with bioinformatic tools. Furthermore, the development of such novel proteomic techniques facilitates the elucidation of the functions of proteins under stress or disease conditions, resulting in the discovery of biomarkers for responses to environmental stimuli. The ultimate objective of proteomics is targeted toward the entire proteome of life, subcellular localization biochemical activities, and the regulation thereof. Comprehensive analysis strategies of proteomics can be classified into three categories: (i) protein separation via 2-dimensional gel electrophoresis (2-DE) or liquid chromatography (LC), (ii) protein identification via either Edman sequencing or mass spectrometry (MS), and (iii) proteome quantitation. Currently, MS-based proteomics techniques have shifted from qualitative proteome analysis via 2-DE or 2D-LC coupled with off-line matrix assisted laser desorption ionization (MALDI) and on-line electrospray ionization (ESI) MS, respectively, toward quantitative proteome analysis. In vitro quantitative proteomic techniques include differential gel electrophoresis with fluorescence dyes. protein-labeling tagging with isotope-coded affinity tags, and peptide-labeling tagging with isobaric tags for relative and absolute quantitation. In addition, stable isotope-labeled amino acids can be in vivo labeled into live culture cells via metabolic incorporation. MS-based proteomics techniques extend to the detection of the phosphopeptide mapping of biologically crucial proteins, which ale associated with post-translational modification. These complementary proteomic techniques contribute to our current understanding of the manner in which life responds to differing environment.

• Bulletin of the Korean Chemical Society
• /
• v.35 no.3
• /
• pp.821-827
• /
• 2014

An isotope dilution liquid chromatography tandem mass spectrometry (ID LC-MS/MS) method has been developed and applied to the determination of arsenobetaine (AsB, ${(CH_3)_3}^+AsCH_2COO^-$) from oyster candidate certified reference material (CRM). The exact matching isotope dilution approach was adopted for accurate determination of AsB using $^{13}C_2$-labeled AsB as an internal standard. Efficiencies of different AsB extraction methods were evaluated using a codfish reference material and a simple sonication method was selected as the method of choice for the certification of the oyster candidate CRM. The hydrophilic interaction liquid chromatography (HILIC) combined with electrospray ionization tandem mass spectrometry (ESI/MS/MS) in selected reaction monitoring (SRM) mode was optimized for adequate chromatographic retention and robust quantification of AsB from codfish and oyster samples. By analyzing 12 subsamples taken from each 12 bottles systematically selected from the whole oyster CRM batch, the certified value of AsB was determined as $6.60mg{\cdot}kg^{-1}{\pm}0.31mg{\cdot}kg^{-1}$ and it showed excellent between-bottle homogeneity of less than 0.42%, which is represented by relative standard deviation of 12 bottles from the CRM batch. The major source of uncertainty was the certified value of the AsB standard solution.

• Korean Journal of Ecology and Environment
• /
• v.46 no.4
• /
• pp.471-487
• /
• 2013

This review paper was written to provide background information as well as future application for aquatic ecologists interested in using stable isotope. Stable isotope techniques has proved to be an extremely useful to elucidate a lot of environmental and ecological problems. Stable isotopes have been used as possible tracers to identify sources, to quantify relative inputs in a system. When utilized carefully, stable isotope tools provides apparent advantages for the scientists to find out the processes of material cycles in various environments and energy flows in natural ecosystems.

• Korean Journal of Ecology and Environment
• /
• v.51 no.3
• /
• pp.245-252
• /
• 2018

Stable isotope tracers were first applied to evaluate the Microcystis cell assimilation efficiency of bivalves, since the past identification method has been limited to tracking the changes of each chl-a, clearity, and nutrient. The filter-feeders (Sinanodonta woodiana and Unio douglasiae) were assessed under the condition of cyanobacteria (Microcystis aeruginosa) blooms through an in mesocosm experiment using $^{13}C$ and $^{15}N$ dual isotope tracers. chl-a concentration in the treatment mesocosm was dramatically decreased after the beginning of the second day, ranging from 116 to $66{\mu}g\;L^{-1}$. In addition, the incorporated $^{13}C$ and $^{15}N$ atom % in the S. woodiana bivalve showed higher values than U. douglasiae bivalves. The results demonstrate that U. douglasiae has less capacity to assimilate toxic cyanobacteria derived from diet. Our results therefore also indicate that S. woodiana can eliminate the toxin more rapidly than U. douglasiae, having a larger detoxification capacity.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
• /
• v.7 no.1
• /
• pp.1-7
• /
• 2002

Optical dating, using infrared stimulation of K-feldspar grains, was undertaken to determine the sedimentation rate of a recently deposited tidal flat on the western coast of Korea. Very low luminescence of the natural surface sample confirms that the materials we investigated were well bleached at deposition. The five IRSL ages obtained show a reasonable stratigraphic correlation with the expected sedimentation rate of approximately 50cm/100yr. In addition the recuperation effect due to thermal transfer was discussed. We conclude that the optical dating used here shows a great promise to determine the sedimentation rates of tidal flats.

• Mass Spectrometry Letters
• /
• v.8 no.3
• /
• pp.59-64
• /
• 2017

In clinical diagnosis, it's well known that the abnormal level of uric acid (UA) in human body is implicated in diverse human diseases, for instance, chronic heart failure, gouty arthritis, diabetes, and so on. As a primary method, an isotope dilution mass spectrometry (IDMS) has been used to obtain the accurate quantity of UA in blood or serum and also develop the certificated reference material (CRM) so as to provide a SI-traceability to clinical laboratories. Due to the low solubility of UA in water, an ammonium hydroxide ($NH_4OH$) has been considered as a promising solvent to increase the solubility of UA that enables the preparation of both UA and its isotope standard solution for next IDMS-based absolute quantification. But, because of using this $NH_4OH$ solvent, it gives rise to the unwanted degradation of UA. In this study, we sought to optimize condition for the stability of UA in $NH_4OH$ solution by varying the mole ratios of UA to $NH_4OH$, followed by ID-LC-MRM analysis. In addition, we also inspected minutely the effect of the storage temperatures. Additionally, we also performed the quantitative analysis of UA in the KRISS serum certificated reference material (CRM, 111-01-02A) with diverse mixing ratios of UA to $NH_4OH$ and then compared those values to its certification value. Based on our experiments, adjusting the mole ratio of 1/2 ($UA/NH_4OH$) with the storage temperature of $-20^{\circ}C$ is an effective way to secure both the solubility and stability of UA in $NH_4OH$ solution for next IDMS-based quantification of UA in serum.