• Title/Summary/Keyword: Isolation unit

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Isolation and Identification of ${\beta}-Glucuronidase$ producing Clostridium sp. from Fecal Microflora (${\beta}-Glucuronidase$ 생산 인체장내 Clostridium sp.의 분리 . 동정)

  • Park, Jong-Hyun;Shin, Jee-Young
    • Korean Journal of Food Science and Technology
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    • v.31 no.5
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    • pp.1357-1362
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    • 1999
  • For the study of human intestinal environments with the intestinal bacteria producing ${\beta}-glucuronidase\;and \;7{\alpha}-dehydroxylase$, genus Clostridium, known as the producer, were isolated from the fecal microflora. Through screening twice for one person, fecal microflora without major bacterial group seemed to be changed, which indicated the microflora would be changeable by the diet factors. With using Neomycin-Nagler selective medium during the screening, 14 Clostridium spp. were isolated and then the harmful enzyme activities were determined. Isolate-11 among them produced strongly ${\beta}-glucuronidase$ and its activity was 0.021 unit/mg Protein. However, the strain producing $7{\alpha}-dehydroxylase$ was not isolated. The Isolate-11 was tentatively identified as Clostridium scatologenes through cultural and physiological characteristic.

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Triplexer based on Filter Characteristics of CRLH Transmission Line and Triple-Band Amplifier Applications (CRLH 전송선로의 필터 특성을 이용한 트리플렉서와 삼중대역 증폭기에의 응용)

  • Yun, Jeong-Ho;Kim, Young;Yoon, Young-Chul
    • Journal of Advanced Navigation Technology
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    • v.16 no.3
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    • pp.433-439
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    • 2012
  • In this paper, we proposed the triplexer using unit-cell of CRLH transmission line which has a bandpass characteristic to reduce adjacent channel interference. The input impedance of triplexer with each channel filter is operated open-circuit at the resonance frequencies of other channels. Such property is due to the combination a series and parallel resonance circuits of CRLH-TL unit-cell. The measured triplexer results are showed a measured insertion loss of each channel, less than 1.5 dB, matching at each port, less than 15dB and isolation between channel, better than 25 dB. Also, to validate the triplexer, a small signal amplifier with triple-band is designed and tested. the measured amplifier results show good agreements with prediction.

Fault Detection and Diagnosis for EVA Production Processes Using AE-SOM (AE-SOM을 이용한 EVA 생산 공정 이상 검출 및 진단)

  • Park, Byeong Eon;Ji, Yumi;Sim, Ye Seul;Lee, Kyu-Hwang;Lee, Ho Kyung
    • Korean Chemical Engineering Research
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    • v.58 no.3
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    • pp.408-415
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    • 2020
  • In this study, the AE-SOM method, which combines auto-encoder and self-organizing map, is used to detect and diagnose faults in EVA production process. Then, the fault propagation pathways are identified using Granger causality test. One year and seven months of operation data were obtained to detect faults of the process, and the process variables of the autoclave reactor are mainly analyzed. In the data pretreatment process, the data are standardized and 200 samples of each grade are randomly chosen to obtain a fault detection model. After that, the best matching unit (BMU) of each grade is confirmed by applying AE-SOM. The faults are determined based on each BMU. When a fault is found, the most causative variable of the fault is identified by using a contribution plot, and the fault propagation pathway is identified by Granger causality test. The prognostic of the two shutdowns is detected, and the fault propagation pathway caused by the faulty variable was analyzed.

Balanced Mixer Based on Composite Right/Left-Handed Transmission Line Leaky-Wave Antenna (CRLH 전송 선로 리키 웨이브 안테나를 이용한 평형 믹서)

  • Kim, Young
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.19 no.9
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    • pp.985-991
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    • 2008
  • This paper presents a novel balanced mixer receiver front-end design based on a metamaterial structure applicable to differential-/common-mode excitation. This metamaterial structure functions as a leaky-wave antenna and provides in-trinsic common-mode suppression. Low LO leakage and high RF to LO isolation are achieved without additional filters for LO and RF paths. The metamaterial is based on a unit-cell which under a differential-mode excitation behaves like a composite right/left-handed(CRLH) metamaterial. In contrast, the metamaterial unit-cell is below cut-off under a common-mode excitation. Experimental results are used to verify the proposed metamaterial's differential-/common-mode characteristics. The metamaterial is integrated with a balanced mixer design resulting in an operation frequency range of $1.96{\sim}2.40$ GHz with an optimum mixer conversion loss of 21.1 dB at 2,4 GHz.

Fault Management Design Verification Test for Electrical Power Subsystem and Attitude and Orbit Control Subsystem of Low Earth Orbit Satellite (저궤도위성의 전력계 및 자세제어계 고장 관리 설계 검증시험)

  • Lee, Sang-Rok;Jeon, Hyeon-Jin;Jeon, Moon-Jin;Lim, Seong-Bin
    • Aerospace Engineering and Technology
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    • v.12 no.2
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    • pp.14-23
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    • 2013
  • Fault management design of the satellite describes preparations for failures which can occur during operational phase. Fault management design contains detection and isolation function of anomaly, and also it contains function to maintain the satellite in safe condition until the ground station finds out a cause of failure and takes a countermeasure. Unlike normal operation, safing operation is automatically performed by Power Control and Distribution Unit and Integrated Bus Management Unit which loads Flight Software without intervention of ground station. Since fault management operation is automatical, fault management logic and functionality of relevant hardware should be thoroughly checked during ground test phase, and error which is similar to actual should be carefully applied without damage. Verification test for fault management design is conducted for various subsystems of satellite. In this paper, we show the design process of fault management design verification test for Electrical Power Subsystem and Attitude and Orbit Control Subsystem of Low Earth Orbit satellite flight model and the test results.

Isolation and Charcterization of Micrococcus sp. HJ-19 Secreting Extracellular Protease (단백질 분해효소를 분비하는 Micrococcus sp. HJ19의 분리 및 특성)

  • Cha, In-Tae;Oh, Yong-Sik;Roh, Dong-Hyun
    • Korean Journal of Microbiology
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    • v.43 no.3
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    • pp.222-226
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    • 2007
  • Proteases are degradative enzymes which hydrolyze a peptide bond between amino acids and they are abundantly applied to commercial field. In order to screen new source of pretense, bacteria secreting extracellular pretense were isolated by enrichment culture from deep sea water samples of East Sea, Korea. A bacterium, named as HJ19, showed the best growth and the largest clear zone in plates supplemented skim milk at $30^{\circ}C$. The partial DNA sequence analysis of the 16S rRNA gene, phenotypic tests and morphology identified that this strain was In genus Micrococcus. The strain HJ19 could not grow at $10^{\circ}C$ but it started growth and showed pretense activity at $20^{\circ}C$. The optimal growth was at $37^{\circ}C$ and the maximal protease activity at $30^{\circ}C$ was about 480unit/ml.

Isolation and Identification of Cellulomonas fimi, Characteristics of its Cellulase and Conversion of the Sawdust into Ethanol (Cellulomonas fimi의 분리(分離) 및 동정(同定), cellulase 특성(特性)과 톱밥의 Ethanol 전환(轉換))

  • Lee, Chan-Yong;Lee, Ke-Ho
    • Applied Biological Chemistry
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    • v.28 no.2
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    • pp.98-105
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    • 1985
  • In the sheep and cattle's rumen, facultative anaerobic cellulolytic bacteria were isolated by using Hungate's roll tube technique. In the 21 isolated species, one was screened by its strong cellulolytic activity and identified as Cellulomonas fimi C-14 by investigate morphological, cultural, physiological characteristics and electron microgram. Optimum conditions of the cell growth and enzyme production were pH 6.5 an $30^{\circ}C$, Thiamine and biotin support a good growth of C. fimi C-14. In the enzyme activities, Crystalline cellulose hydrolyzing activity, CMCase activity and ${\beta}-glucosidase$ activity were 20.6, 226.6 and 0.56$(unit{\times}10^3/ml)$ at pH 6.0, $40^{\circ}C$. By addition of fungal cellulase, enzyme activity was increased. Simultaneous Saccharification Fermentation is better than two step fermentation in ethanol yield with Saccharomyces cerevisiae DY2.

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Identification of a novel triterpene saponin from Panax ginseng seeds, pseudoginsenoside RT8, and its antiinflammatory activity

  • Rho, Taewoong;Jeong, Hyun Woo;Hong, Yong Deog;Yoon, Keejung;Cho, Jae Youl;Yoon, Kee Dong
    • Journal of Ginseng Research
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    • v.44 no.1
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    • pp.145-153
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    • 2020
  • Background: Panax ginseng Meyer (Araliaceae) is a highly valued medicinal plant in Asian regions, especially in Korea, China, and Japan. Chemical and biological studies on P. ginseng have focused primarily on its roots, whereas the seeds remain poorly understood. This study explores the phytochemical and biological properties of compounds from P. ginseng seeds. Methods: P. ginseng seeds were extracted with methanol, and 16 compounds were isolated using various chromatographic methods. The chemical structures of the isolates were determined by spectroscopic data. Antiinflammatory activities were evaluated for triterpene and steroidal saponins using lipopolysaccharide-stimulated RAW264.7 macrophages and THP-1 monocyte leukemia cells. Results: Phytochemical investigation of P. ginseng seeds led to the isolation of a novel triterpene saponin, pseudoginsenoside RT8, along with 15 known compounds. Pseudoginsenoside RT8 exhibited more potent antiinflammatory activity than the other saponins, attenuating lipopolysaccharide-mediated induction of proinflammatory genes such as interleukin-1β, interleukin-6, inducible nitric oxide synthase, cyclooxygenase-2, and matrix metalloproteinase-9, and suppressed reactive oxygen species and nitric oxide generation in a dose-dependent manner. Conclusion: These findings indicate that pseudoginsenoside RT8 has a pharmaceutical potential as an antiinflammatory agent and that P. ginseng seeds are a good natural source for discovering novel bioactive molecules.

Isolation and Identification of Protease Producing Bacteria in Kimchi (김치에서 단백질분해효소활성 균주분리 및 동정)

  • Min, Sung-Gi;Kim, Jung-Hee;Kim, Tae-Woon;Kim, Kyung-Nam
    • Korean Journal of Food Science and Technology
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    • v.35 no.4
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    • pp.666-670
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    • 2003
  • Strains producing the protease, which is essential for growth of lactic acid bacteria and fermented kimchi, were screened and identified. Among five types of selected pulmuone kimchis (Jeonlado kimchi, ripened kimchi, yeolmu kimchi, kakdugi, and baechu kimchi), nine strains of bacteria were screened and identified by whole cell protein pattern and API test. The nine strains consisted of one of Lactobacillus sp., one of Leuconostoc sp., six of Bacillus sp., and one of Brevibacillus sp. The protease activities of these strains were compared with known strains (Bac. subtilis KCCM 12248 and Bac. licheniformis KCCM 11851) producing protease. Among tested strains, K-2 (Brevibacillus sp.) showed the highest value (0.11 unit/mg) in protease activity.

GPGPU Task Management Technique to Mitigate Performance Degradation of Virtual Machines due to GPU Operation in Cloud Environments (클라우드 환경에서 GPU 연산으로 인한 가상머신의 성능 저하를 완화하는 GPGPU 작업 관리 기법)

  • Kang, Jihun;Gil, Joon-Min
    • KIPS Transactions on Computer and Communication Systems
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    • v.9 no.9
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    • pp.189-196
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    • 2020
  • Recently, GPU cloud computing technology applying GPU(Graphics Processing Unit) devices to virtual machines is widely used in the cloud environment. In a cloud environment, GPU devices assigned to virtual machines can perform operations faster than CPUs through massively parallel processing, which can provide many benefits when operating high-performance computing services in a variety of fields in a cloud environment. In a cloud environment, a GPU device can help improve the performance of a virtual machine, but the virtual machine scheduler, which is based on the CPU usage time of a virtual machine, does not take into account GPU device usage time, affecting the performance of other virtual machines. In this paper, we test and analyze the performance degradation of other virtual machines due to the virtual machine that performs GPGPU(General-Purpose computing on Graphics Processing Units) task in the direct path based GPU virtualization environment, which is often used when assigning GPUs to virtual machines in cloud environments. Then to solve this problem, we propose a GPGPU task management method for a virtual machine.