• Microbiology and Biotechnology Letters
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• v.23 no.3
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• pp.297-303
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• 1995

Antitumor antibiotic material was produced by Streptomyces sp. YBE-316 which was isolated from soil, and the optimal culture conditions for the antitumor antibiotic material production were as follows; 2.0% (w/v) sucrose, 0.8% (w/v) polypeptone, 0.4% (w/v) yeast extract, 0.2% (w/v) K$_{2}$HPO$_{4}$, pH 7.0, at 30$\circ$C, 150 rpm and for 100 hours culture. The antitumor antibiotic material had strong antitumor antibiotic activities against most testing tumor cell lines, gram positive and negative bacteria, yeasts, and, especially, Penicillium chrysogenum in fungi.

• Microbiology and Biotechnology Letters
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• v.20 no.6
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• pp.630-636
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• 1992

Hansenula nonfermentans KYP-l was selected and identified from 19 methanol utilizing yeasts isolated from soil samples by the enrichment culture technique. This strain showed a high cell concentration and a high aldehyde production. Aldehyde production was carried out in a resting cell system using methanol utilizing yeast as a biocatalyst. The molar yield of acetaldehyde was the highest among the aldehyde investigated, and the maximum amount of aldehyde was produced by cells obtained from a 40 hours' culture.

• KSBB Journal
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• v.15 no.6
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• pp.560-565
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• 2000

13-Deacetyl-taxchinin I, a taxoid having a rearranged 11(15\longrightarrow1)-abeo-taxane skeleton, has been isolated and identified from plant cell cultures of Taxus chinensis. The compound has not previously been encountered in nature. Its structure was elucidated by 1-and 2D NMR techniques including H-H COSY, HMQC, and HMBC experiments. This taxoid also provides information for better understanding of structure-activity relationships and biosynthesis, as well as improving the quality control of paclitaxel production.

• International Journal of Automotive Technology
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• v.8 no.2
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• pp.233-241
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• 2007

A lumped parameter model is proposed for the analysis of dynamic behaviour of a Passive Hydraulic Engine Mount (PHEM), incorporating inertia track and throttle, which is characterized by effective and efficient vibration isolation behaviour in the range of both low and high frequencies. Most of the model parameters, including volume compliance of the throttle chamber, effective piston area, fluid inertia and resistance of inertia track and throttle are identified by an experimental approach. Numerical predictions are obtained through a finite element method for responses of dynamic stiffness of the rubber spring. The experiments are made for the purpose of PHEM validation. Comparison of numerical results with experimental observations has shown that the present PHEM achieves good performance for vibration isolation.

• The Plant Pathology Journal
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• v.34 no.3
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• pp.157-162
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• 2018

The objective of this work was to accomplish the isolation, molecular identification and characterizing the physiology of the causal agent of the algal spot in mango trees. For this purpose, the pathogen growth was assessed in different culture media, with subsequent observation and measurements of the filamentous cells. The molecular identification was made using mycelium obtained from leaf lesions and pure algae colonies grown in culture medium. Descriptions based on DNA sequencing indicated that the algae is Cephaleuros virescens. The algae must be isolated primarily in liquid medium for further pricking into agar medium. The highest mycelial growth average in Petri dishes occurred when the algae were grown in Trebouxia and BBM. Trebouxia enabled larger cells in the filamentous cells when compared to other culture media.

• Biomedical Science Letters
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• v.16 no.3
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• pp.133-138
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• 2010

Despite of the increasing importance of environmental mycobacteria, detection and identification of mycobacteria from environmental sources including water have been fraught with technical difficulties. Although, several protocols to optiruize isolation of mycobacteria from water sources have been reported, standard method has not yet been established. In this study, usefulness of cetylpyridinium chloride (CPC), a cationic quaternary ammonium compound, for the isolation of environmental mycobacteria from diverse water samples was evaluated. For this, water samples from diverse water sources such as effluent water, lake water, and underground water were collected, treated with diverse concentrations of CPC, and plated on the solid agar plates. Subsequently individual colonies grown on the plates were sequence analyzed for identification of each colony. In brief, the results from this study showed that the growth of mycobacteria was enhanced by use of CPC as a pre-treatment reagent to water samples by inhibiting growth of other non-mycobacteria in water. In fact, the effect of CPC to decontaminate non-mycobacteria for isolation of mycobacteria was better than 1~4% of NaOH, which is a routinely used decontaminating reagent widely employed for culturing mycobactera from sputum specimens. Therefore, the results from this study seems to support that the CPC pre-treatment may be useful for isolation of mycobacteria from diverse sources including clinical specimens which are often contaminated with other bacteria.

• YAKHAK HOEJI
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• v.50 no.1
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• pp.21-25
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• 2006

Reiterated normal-phase column chromatography lead to the isolation and purification of six known compounds but for the first time from the whole plant of Sasa borealis (Hack.) Makino (Gramineae): tricin 4'-O-(erythro-${\beta}$-guaia-cylglyceryl) ether (1), tricin 4'-O-(threo-${\beta}$-guaiacylglyceryl) ether (2), tricin 4'-O-[erythro-${\beta}$-guaiacyl-(9'-O-acetyl)-glyceryl] ether (3), tricin 4'-O-[threo-${\beta}$-guaiacyl-(9'-O-acetyl)-glyceryl] ether (4), (-)-pinoresinol (5), and vanillin (6). The structures of the compounds (1-6) were established based on interpretation of high resolution NMR (COSY, HSQC, HMBC, and NOESY) spectral data. In particular, compounds 1 and 3 were diastereomers of compounds 2 and 4, respectively. These two sets of diastereomers were able to be simultaneously identified and quantified by a gradient reversed-phase HPLC method with UV photodiode array, This sensitive HPLC method is noteworthy as a simultaneous separation and identification method to test the extract of the family Gramineae which contains these compounds.

• Journal of Applied Biological Chemistry
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• v.50 no.1
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• pp.29-31
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• 2007

• Proceedings of the Korean Society of Food and Cookery Science Conference
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• 1997.05a
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• pp.255-255
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• 1997

• Korean Journal of Environmental Agriculture
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• v.28 no.4
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• pp.442-446
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• 2009

This study was undertaken to isolate and to identify a fungal pathogen Colletotrichum sp. KV-21 associated with apple anthracnose. Rotted Gala apples were used for the isolation of the fungus. The infected tissues were sterilized with 70% ethanol, washed with sterilized distilled water and were transferred to 50 ml containing potato broth (PDB) flasks. The peripheral hyphae of the fungal colony which developed from the infected tissues were isolated on to potato dextrose agar (PDA). On PDA plates the fungus grew well at $25^{\circ}C$ and occupied more than half of a 9 cm petri dish within 5 days. The fungal cultures on PDA were used for morphological observation and identification of the fungus. Conidiophores were produced on the gray to whitish sporodochial structures scattered on PDA plates which gave rise to conidiogenous cells. The structures of the conidia produced on PDA plates were subcylindrical to obovoid, fusoid, tapered and 4 to $6\;{\mu}m$ in size.