• Title/Summary/Keyword: Isolation Test

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Epidemiological aspects of Salmonella spp infections of domestic animals in Gyeongbuk province (경북지역 가축에서 Salmonella속 균 감염증에 대한 역학적 특성)

  • Kim, Sang-Yun;Lee, Hee-Moo;Kim, Sin;Hong, Hyon-Pyo;Kwon, Heon-Il
    • Korean Journal of Veterinary Service
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    • v.24 no.1
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    • pp.51-68
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    • 2001
  • The result of studying the epidemiological characteristics of Salmonella strains which have been isolated from the domestic animals in Gyeongbuk province from February 1998 to August 2000 were summarized as follows. The isolation rates of Salmonella strains were 2.0% from cattle feces, 6.3% from cattle lymph node, 9.5% from pig feces, and 25.1% from pig lymph node. In poultry, the isolation rates were 30.3%. The isolates of Salmonella showed positive reaction for MUCAP test, methyl red test, but showed negative reaction for urea test, indole test, Voges Proskauer test. On TSI agar, the isolates showed acid butt, alkaline slant. Also, the isolates were identified as Salmonella strain by API 20E kit. Non H$_2$S Production Salmonella strains isolated from poultry were identified as S gallinarum. As a result of serotyping, B group were the most common in cattle and pig, Dl in chickens. 21 serovars were found. the common serovar from the domestic animals was S typhimurium, S derby, S agona, S schwarzenground, S enteritidis and S gallinarum. The most commonly encountered serovars in cattle were S agona and S typhirimurium in pig, S gallinarum in chicken. As a result of antimicrobial susceptibility test, all Salmonella isolates were susceptible to amikacin, ciprofloxacin, norfloxacin; cefotaxime and polumcin B. The resistance rates to tetracycline and streptomycin was 58% and 56%, respectively. 69.3% of all isolates were resistant to more than one antimicrobial agent. Out of the resistant isolates, the isolates resistant to streptomycin and tetracycline was 36%. There were 24 strains of multiresistant isolates resistant to more than 5 antimicrobial agents. S typhimurium were resistant to all antimicrobial agents, also had a lot of multiresistant strains. Therefore, S typhimurium was considered as a major agent of antimicrobial resistance.

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The Investigative Study on the Small-sized Isolation Device: regarding of the isolation performance and function test In Emergency Disaster Circumstances (재난현장 소형격리장비의 성능 및 기능평가에 관련된 탐색적 연구)

  • Choi, Jin Woo;Haam, Sunnie;Yoon, Myuong O
    • Fire Science and Engineering
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    • v.34 no.1
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    • pp.135-141
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    • 2020
  • The first case of Middle East Respiratory Syndrome (MERS) was reported in Korea in 2015, after a Korean man from Bahrain tested positive for the infection. After first eruption, there were numbers of secondary infectees and whole country were frightened. At the time, isolated ambulances were available but few, and there was no specific way to prevent contamination during transfer. Therefore, Seoul National University Hospital and the University of Seoul developed isolation units and air filters. Four years after the MERS outbreak, this study measured the differential pressure inside and outside the small isolation units based on the differential pressure and air flow derived from simulation and testing, and proposed minimum values for virus leakage and internal sealing. A performance evaluation and testing method for the transfer equipment is proposed. The critical function of the small isolated transport equipment was defined and selected as an evaluation item; performance evaluation was carried out by simulating a real-world case. The results provide the proper pressure configuration of positive and negative pressure inside the segregation feeder; the clear criteria for the HEPA filter; and the performance criteria for the segregation feeder and air purifier.

Isolation and Characteristics of Composting-promoting-bacteria (부숙촉진 미생물 분리 및 분리균의 특성)

  • Lee, Young-Han;Park, Sang-Ryeol
    • Korean Journal of Soil Science and Fertilizer
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    • v.34 no.6
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    • pp.394-400
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    • 2001
  • This experiment was conducted to determine the safety and feasibility of using compost-promoting-bacteria. Compost-promoting-bacteria was isolated from livestock compost containing sawdust. The isolated bacteria was identified as Bacillus subtilis LYH201 by the method of the composition of the fatty acid with MIDI system and Bergey's manual. This Bacillus subtilis LYH201 had the following characteristics : Gram-positive, straight rod ($0.5{\sim}0.7{\mu}m$ width, $2.5{\sim}3.0{\mu}m$ length), facultatively aerobic and product of xylanase, CMCase, catalase, oxidase, protease and $0.5{\sim}0.7{\mu}m$-amylase. Growth of Bacillus subtilis LYH201 at saccharose as carbon source(0.5%) was faster than other carbon source. Activity of cellulase. $0.5{\sim}0.7{\mu}m$-amylase and protease from Bacillus subtilis LYH201 after 24 hours at $50^{\circ}C$ by agar diffusion method was higher than that of low temperature. Optimum growth condition of Bacillus subtilis LYH201 was $50^{\circ}C$ and pH 6.

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Study on the Effect of Vibration Reduction Characteristics of Floors on the Inside Running Noise in Railway Vehicles (전동차 바닥구조의 진동절연특성이 실내소음에 미치는 영향)

  • Woo, Kwan-Je
    • Proceedings of the KSR Conference
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    • 2008.06a
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    • pp.908-912
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    • 2008
  • Structure-borne noise is known to dominate internal running noise levels in open field. In this paper vibration isolation characteristics of floors are investigated to see their effect on the internal running noise. By using the analysis and test results of test specimen of small size, internal running noise level of complete train is estimated. Analysis results show that soft rubber as a vibration isolator has the most favorable effect on the inside running noise.

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Isolation of Anaplasma centrale from Korean Cattle (아나플라즈마병(病)에 관(關)한 연구(硏究) 제(第) II 보(報) 한우(韓牛)에서 Anaplasma centrale 분리(分離))

  • Jeon, Yeong
    • Korean Journal of Veterinary Research
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    • v.18 no.1
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    • pp.19-22
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    • 1978
  • In order to isolate and identify Anaplasma bodies, two dairy cattle were inoculated with the whole blood of Korean cattle reacted to capillary tube agglutination test (Ana-test). The results obtained are as follows: 1. In the infected dairy cattle, Anaplasma bodies were first detected between 30 to 40 days post infection. 2. The isolates were identified as Anaplasma centrale on the basis of morphology of infected anaplasma bodies in erythocytes.

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Design and Performance Test of Rubber Mounts for Shock-Isolation (고무를 이용한 완충요소의 설계 및 성능시험)

  • 유춘화;권형오;이신언
    • Journal of KSNVE
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    • v.2 no.1
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    • pp.41-47
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    • 1992
  • In order to reduce the shock which may occur during shipping of a drumtype container, rubber mounts were designed using a commercial FEM package and manufactured, and then the performance was checked by static and dynamic test. According to the design specifications, the container system was tested by dropping. The experimental results are compared with the theoretical ones.

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Isolation of kpn I restriction endonuclease from klebsiella pneumonia (Klebsiella pneumonia로 부터 제한효소 Kpn I의 분리)

  • 이상철;이대실;유명희
    • Korean Journal of Microbiology
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    • v.25 no.1
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    • pp.73-79
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    • 1987
  • A restriction endonuclease, Kpn I has been isolated from Klebsiella pneumonia. Cells were broken by sonication. After ultracentrifugation the supernatant containing Kpn I activity was further purified by Sepharose-6B gel filtration, DEAD-Cellulose, Heparin-Agarose, and Aminohexyl-Agarose column chromatography. Final enzyme preparation was essentially free of contamination exonuclease and phosphatase, as judged by ligation-recut test. Total activity of the enzyme recovered from 10 grams of cells was $4.7\times 10^5$ units.

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Development and Application of Educational Equipment for Vibration Engineering (진동공학 교육장비 개발 및 적용)

  • Rim, Kyung-Hwa;Yang, Xun
    • The Journal of Korean Institute for Practical Engineering Education
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    • v.1 no.1
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    • pp.61-65
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    • 2009
  • Due to the less variety of experiment equipments for engineering education, the development of designated educational equipment is really in urgent need at most time. Especially for the in-depth engineering major subject, vibration engineering, there are almost no related educational equipments in Korea. Accordingly, in order to simulate the vibration phenomena occurred in the actual field, the development of educational equipment for vibration engineering theory and experiment education is required. This paper introduces four development educational equipments developed in graduated research works, which are critical speed test educational equipment, balancing machine educational equipment, isolation educational equipment using eddy current and active vibration isolation educational equipment. Through using them in experiment lesson, the utilization and practicality of educational equipment are verified. The proposed improvement for future development and application of educational equipment is shown in addition.

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Sequential Fault Detection and Isolation for Redundant Inertial Sensor Systems with Uncertain Factors

  • Kim, Jeong-Yong;Yang, Cheol-Kwan;Shim, Duk-Sun
    • 제어로봇시스템학회:학술대회논문집
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    • 2003.10a
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    • pp.2594-2599
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    • 2003
  • We consider some problems of the Modified SPRT(Sequential Probability Ratio Test) method for fault detection and isolation of inertial redundant sensor systems and propose an Advanced SPRT method to solve the problems of the Modified SPRT method. One problem of the Modified SPRT method to apply to inertial sensor system comes from the effect of inertial sensor errors such as misalignment, scale factor error and sensor bias in the parity vector, which make the Modified SPRT method hard to be applicable. The other problem is due to the correlation of parity vector components which may induce false alarm. We use a two-stage Kalman filter to remove effects of the inertial sensor errors and propose the modified parity vector and the controlled parity vector which removes the effect of correlation of parity vector components. The Advanced SPRT method is derived form the modified parity vector and the controlled parity vector. Some simulation results are presented to show the usefulness of the Advanced SPRT method to redundant inertial sensor systems.

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Distribution and Antibiotic Resistance of Vibrio vulnificus isolated in Incheon Coastal Area (인천지역 해양환경에서 분리된 비브리오패혈증군의 분포 및 항생제 내성 양성)

  • Hwang, Kyoung-Wha;Oh, Bo-Young;Gong, Young-Woo;Lee, Jae-Mann;Go, Jong-Myoung;Kim, Yong-Hee
    • Journal of environmental and Sanitary engineering
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    • v.22 no.4
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    • pp.55-63
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    • 2007
  • We investigated the distribution and drug susceptibility test of V. vulnificus isolated from environmental sources in Incheon. In this survey, total 4,302 samples were obtained from different sites of the Incheon coastal area during the periods from march 2004 to November 2006. Among the 4,302 samples, 310 strains were isolated. The isolation rates of Vibrio vulnificus from fish, shellfish, estuarine water and sediment were 6.7%, 4.7%, 12.4% and 23.2%, respectively. The highest isolation rate was 14.6% in September and in spite of low temperature the isolation rate was 5.1% in November. The highest rate of antibiotic resistance was observed against streptomycin(15.3%), cefazolin(8.5%), cephalothin(8.3%), amikacin(8.3%), cefoxitin(6.7%) and nalidixic acid(6.7%). Seventeen percent of isolates were observed to be resistant to two or more of the antibiotics tested.