• Asian-Australasian Journal of Animal Sciences
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• v.31 no.1
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• pp.3-12
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• 2018

Objective: DNA methylation plays a major role in regulating the expression of genes related to traits of economic interest (e.g., weight gain) in livestock animals. This study characterized and investigated the functional inferences of genome-wide DNA methylome in the loin (longissimus dorsi) muscle (LDM) of swine. Methods: A total of 8.99 Gb methylated DNA immunoprecipitation sequence data were obtained from LDM samples of eight Duroc pigs (four pairs of littermates). The reference pig genome was annotated with 78.5% of the raw reads. A total of 33,506 putative methylated regions (PMR) were identified from methylated regions that overlapped at least two samples. Results: Of these, only 3.1% were commonly observed in all eight samples. DNA methylation patterns between two littermates were as diverse as between unrelated individuals (p = 0.47), indicating that maternal genetic effects have little influence on the variation in DNA methylation of porcine LDM. The highest density of PMR was observed on chromosome 10. A major proportion (47.7%) of PMR was present in the repeat regions, followed by introns (21.5%). The highest conservation of PMR was found in CpG islands (12.1%). These results show an important role for DNA methylation in species- and tissue-specific regulation of gene expression. PMR were also significantly related to muscular cell development, cell-cell communication, cellular integrity and transport, and nutrient metabolism. Conclusion: This study indicated the biased distribution and functional role of DNA methylation in gene expression of porcine LDM. DNA methylation was related to cell development, cell-cell communication, cellular integrity and transport, and nutrient metabolism (e.g., insulin signaling pathways). Nutritional and environmental management may have a significant impact on the variation in DNA methylation of porcine LDM.

• Journal of Microbiology and Biotechnology
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• v.27 no.11
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• pp.2052-2059
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• 2017

The emergence and dissemination of Salmonella genomic island 1 (SGI1) are strongly associated with the occurrence of multidrug-resistant (MDR) enterobacteria in humans and animals. Diverse SGI1s have been reported among Salmonella enterica and Proteus mirabilis in several countries. We aimed to characterize SGI1 in P. mirabilis isolates from humans and chickens in Chungcheong Province, Korea. A total of 44 P. mirabilis isolates were recovered from humans (n = 20) and chickens (n = 24). Antimicrobial susceptibility was determined by disk diffusion assay. To detect and characterize SGI1s, PCR amplification and PCR mapping experiments were performed. Repetitive extragenic palindromic-PCR (REP-PCR) was performed to assess the clonality of the isolates. The four P. mirabilis strains (16.7%) from chicken harbored a SGI1, whereas none of the isolates from clinical specimens contained SGI1. The SGI1s detected in our study were all confirmed as SGI1-PmABB harboring aminoglycoside-resistant genes (aacCA5 and aadA7). In P. mirabilis isolates, the presence of SGI1-PmABB was significantly correlated with high resistance rates of the isolates to antimicrobial agents, such as gentamicin, streptomycin, and spectinomycin. Moreover, the four SGI1-bearing isolates showed the same REP-PCR patterns and that suggested both horizontal and clonal spread of the isolates. This study is the first attempt to determine SGI1s in P. mirabilis isolates in Korea. We confirmed that P. mirabilis isolates carrying SGI1-PmABB were distributed at poultry farms in Korea. The present study emphasizes the need for continuous monitoring of SGI1s to prevent spreading of the MDR genomic islands among P. mirabilis isolates from humans and animals.

• Korean Journal of Head & Neck Oncology
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• v.33 no.1
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• pp.21-29
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• 2017

Methylation of CpG islands in the promoter region of genes acts as a significant mechanism of epigenetic gene silencing in head and neck squamous cell carcinoma (HNSCC). DNA methylation markers are particularly advantageous because DNA methylation is an early event in tumorigenesis, and the epigenetic modification, 5-methylcytosine, is a stable mark. In the present study, we assessed the genome-wide preliminary screening and were to identify novel methylation biomarker candidate in HNSCC. Genome-wide methylation analysis was performed on 10 HNSCC tumors using the Methylated DNA Isolation Assay (MeDIA) CpG island microarray. Validation was done using immunohistochemistry using tissue microarray of 135 independent HNSCC tumors. In addition, in vitro proliferation, migration/invasion assays, RT-PCR and immunoblotting were performed to elucidate molecular regulating mechanisms. Our preliminary validation using CpG microarray data set, immunohisto-chemistry for HNSCC tumor tissues and in vitro functional assays revealed that methylation of the Homeobox B5 (HOXB5) and H6 Family Homeobox 2 (HMX2) could be possible novel methylation biomarkers in HNSCC.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.23 no.11
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• pp.848-853
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• 2010

We have grown GaN nanowires by the low pressure MOCVD method on Ni deposited oxidized Si surface and have established optimum conditions by observing surface microstructure and its photoluminescence. Optimum growth temperature of $880^{\circ}C$, growth time of 30 min, TMG source flow rate of 10 sccm have resulted in dense nanowires on the surface, however further increase of growth time or TMG flow rate has not increased the length of nanowire but has formed nanocrystals. On the contrary, the increase of ammonia flow has increased the length of nanowires and the coverage of nanowire over the surface. The shape of nanowire is needle-like with a Ni droplet at its tip; the length is tens of micron with more than 40 nm in diameter. Low temperature photoluminescence obtained from the sample at optimum growth condition has revealed several peaks related to exciton decay near band-edge, but does not show any characteristic originated from one dimensional quantum confinement. Strong and broad luminescence at 2.2 eV is observed from dense nanowire samples and this suggests that the broad band is related to e-h recombination at the surface state in a nanowire. The current result is implemented to the nanowire device fabrication by nanowire bridging between micro-patterned neighboring Ni catalysis islands.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• v.5 no.2
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• pp.37-42
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• 2024

This study used a GPS tracker to determine characteristics of wintering habitat use by black-tailed gulls on the west coast of Korea. Results of this study showed a southward movement in December and a northward movement in March. The wintering habitat was confirmed to include the southwest coastal island area (Group 1), the inland coastal area (Group 2), and China (Group 3). The wintering home range was the narrowest in October and the widest in December. Home range of Group 1 was increased from November, showing the maximum in December, decreased in February, and then tended to increase again in March. Group 2 showed the widest home range in November and a narrow range of home range from December. Home range of Group 3 showed a mixed pattern of group 1 and group 2. The flight rate was the lowest in January and the highest in March. Day and night flight rates showed significant differences by month. This study identified wintering characteristics of the West Coast population of black-tailed gulls on the West Coast, including their wintering areas, migration periods, and use patterns. These wintering characteristics of black-tailed gull in the west coast area are thought to be affected by environmental factors (such as food resources and fishing activities) and geographical characteristics of the West Coast where many islands are distributed.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2021.10a
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• pp.167-169
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• 2021

There are only 14 public medical facilities available to residents regularly in the island area of Chungcheongnam-do, one health life support center, and one hospital ship, and these facilities and equipment cannot effectively respond to emergency patients such as severe trauma, cerebrovascular and cardiovascular diseases. The fastest means of transporting emergency patients is to use a doctor helicopter deployed at Dankook University Hospital (based in Cheonan). However, there is only one doctor helicopter deployed, and it cannot operate at night, and since it is in charge of all areas of Chungcheongnam-do, there is a limit to rapid transport in the event of a large number of patients. Therefore, we would like to review the necessity of "Chungnam-type Doctor UAM Development" to compensate for the problems of the urban aviation mobility (UAM) industry and the emergency patient transport system in islands, a field of the 4th industry.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2009.11a
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• pp.114-114
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• 2009

ZnO has received considerable attention due to its potential applicability to optoelectronic devices such as ultraviolet-light emitting diodes (UVLEDs) and laser diodes (LDs). As well known, however, polar ZnO with the growth direction along the c-axis has spontaneous and piezoelectric polarizations that will result in decreased quantum efficiency. Recently, nonpolar ZnO has been studied to avoid such a polarization effect. In order to realize applications of nonpoar ZnO-based films to LEDs, growth of high quality alloys for quantum well structures is one of the important tasks that should be solved. $Mg_xZn_{1-x}O$ and $Cd_xZn_{1-x}O$ is ones of most promising alloys for this application because the alloys of ZnO with MgO and CdO provide a wide range of band-gap engineering spanning from 2.4 to 7.8 eV. In this study, we investigated on $Mg_xZn_{1-x}O$ films grown with various Mg/Zn flux ratios The films were grown on R-plane sapphire substrates by plasma-assisted molecular beam epitaxy (PAMBE). we investigated on $Mg_xZn_{1-x}O$ films grown with various Mg/Zn flux ratios. The films were grown on R-plane sapphire substrates by plasma-assisted molecular beam epitaxy (PAMBE). With the relatively low Mg/Zn flux ratios, a typical striated anisotropic surface morphology which was generally observed from the nonpolar (11-20) ZnO film on r-plane sapphire substrates. By increasing the Mg/Zn flux ratio, however, additional islands were appeared on the surface and finally the surface morphology was entirely changed, which was generally observed for the (0001) polar ZnO films by losing the striated morphology. Investigations by X-ray $\Theta-2{\Theta}$ diffraction revealed that (0002) and (10-11) ZnO planes are appeared in $Mg_xZn_{1-x}O$ films by increasing the Mg/Zn flux ratio. Further detailed investigation by transmission electron microscopy (TEM) and photoluminescence (PL) will be discussed.

• Journal of the Korean Society of Combustion
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• v.20 no.1
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• pp.6-14
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• 2015

Numerical study was conducted to clarify effects of added $H_2O$ for the downstream interaction between $H_2$-air and CO-air premixed flames in counterflow configuration. The reaction mechanism adopted was Davis model which had been known to be well in agreement with reliable experimental data. The results showed that both lean and rich flammable limits were reduced in increase of strain rate. The most discernible difference between the two with and without having $H_2O$ and/or $H_2$ addition into $H_2$-air and CO-air premixtures was two flammable islands for the former and one island for the latter at high strain flame conditions. Even a small amount of $H_2$, in which $H_2$-air premixed flame cannot be sustained by itself, participates in CO oxidation, thereby altering the CO-oxidation reaction path from the main reaction route $CO+O_2{\rightarrow}CO_2+O$ with a very long chemical time in CO-air flame to the OH-related reaction routes including $CO+OH{\rightarrow}CO_2+H$ with very short chemical times. This intrinsic nature alters flame stability maps appreciably. The results also showed that chemical effects of added $H_2O$ help lean flames at relatively low strain rate be sustained, and suppress the flame stabilization at high strain rates.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.19 no.3
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• pp.11-27
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• 2016

This study is a survey of the flora distributed in Seocheon tidal flat wetland protected area of nearby island (Daejuk-do, Tokki-deung, Muk-do) and Yubo-do Seocheon-gun Chuncheongnam-do, Korea. The numbers of vascular plants were summarized as 231 taxa including 65 families, 167 genera, 204 species, 2 subspecies, 23 varieties and 2 forms. The halophytes were 33 taxa including 15 families, 29 genera, 29 species, 3 varieties and 1 form (Chenopodium album var. stenophyllum, Cnidium japonicum, Juncus haenkei and so on). The rare plants were 4 taxa including Pseudoraphis ukishiba (VU), Belamcanda chinensis (VU), Phacelurus latifolius (LC) and Polygonum bellardii (DD). The Korean endemic plants were 2 taxa including Salix pseudolasiogyne and Forsythia koreana. In the specific plants by floristic region were 24 taxa, a degree I were 19 taxa (Rhodotypos scandens, Zanthoxylum planispinum, Cynodon dactylon and so on), 2 taxa of a degree III (Elymus mollis and Asparagus oligoclonos), 3 taxa of a degree IV (Polygonum bellardii, Wisteria floribunda and Pseudoraphis ukishiba) and degree II, V were not found. The naturalized plants were 40 taxa including Phytolacca americana, Sicyos angulatus, Lactuca scariola and so on. Naturalization rate (NR) was 17.3% of all 231 taxa of vascular plants and urbanization index (UI) was 12.5% of all 321 taxa of naturalized plants. This study was performed to used as a basic data to identify the protected from plant ecosystems of type classify into analyze the vegetation characteristic of based on flora distributed in Yubu-do and nearby islands.

• Journal of the Economic Geographical Society of Korea
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• v.8 no.1
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• pp.153-169
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• 2005

In this study, we analysed the state of the fisheries in Chungnam around 1910 from Hangook Susanji. As a result, there were about 30 ports in the west coast area in Chungnam, and we can classify their locations into 5 types Type I is located in small and mid sized rivers. Type II is located in a costal port. Type III is ports facing the open sea, and type IV is ports in a bay. Type V is ports on islands of coast. Species of fish are porges, mackerels, sting rays, croakers, hairtails, shrimps, etc. The base of fishing porge was Jukdo and Yeondo. The fishing banks of croakers are formed from Chilsantan, Jeonlado to the area of seashore, Chungnam. Hairtails were caught near all the areas of seashore. Salt manufacturing is done near all coasts of Chungnam, Seocheon-bay, Ungcheon-bay, Ocheon-bay, Anmyeon island, Taean, Dangjin area, etc represent this work. Especially, the salt which was produced in Taeangun was so famous that it was called ' Taeansalt'. In the Kum-river area there were 32 fishing villages. Freshwater fish were caught in the upper stream area and the mouth of Geumgang, down stream, was the center for producing Baengeo. There were 23 fishing villages, and the species consisted of croaker, hairtail, Forge, gray mullet, sting ray, sharks, anchovy, shrimps, etc in Biin-bay. The coast of Boryeong had about 33 fishing villages, but their fishing industry was not developed more than their good salt manufacturing. Though there are records that Chunsu-bay had 28 fishing villages, Taean peninsula and Garolim-bay had about 70 fishing villages, and Asan-bay area had about 55 fishing villages, they were developed a little, but not more than Weir fisheries or salt manufacture on the base of salt area and the tideland because these areas were just for farming. South of Anmyeon island of West coast island areas is the biggest area of Stow nets on stakes fishing and the island areas down mouth of Kum river were centers for producing porge.