• Applied Microscopy
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• 제21권1호
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• pp.46-62
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• 1991

The intermediate filament is one of the most important constituents of the intracytoplasmic cytoskeleton microtubule, actin, myosin and intermediate filament. It is composed of keratin, desmin, vimentin, neurofilament and glial filament, and has important role as a cellular marker, epithelial or mesenchymal origin. So it will be important to differentiated from some poorly or undifferentiated neoplasm to provide adequate therapeutic modalities. This study was performed by using immunohistochemical staining and electron microscopic observation to find out intermediate filaments of epithelial and non-epithelial tumor cells evaluate the degree of differentiation in tumors and therefore to provide some diagnostic and therapeutic modalities. The materials consisted of 83 epithelial and non-epithelial elements bearing 23 normal control, 28 epithelial tumors, and 32 non-epithelial tumors, that are resected for definite treatment at Chosun University Hospital from June, 1988 to June, 1990. Immunohistochemical stain for keratin, desmin and vimentin, and electron microscopic study were performed in all cases. The results obtained were as follows. 1. Immunohistochemical stain for intermediate filament were very useful diagnostic aid for differentiated epithelial tumor to non-epithelial tumor in diagnostic neoplasia. 2. In the electron microscopic finding, the size of intermediate filaments were possible differentiated to cell components of epithelial tumor and non-epithelial tumors.

• 천문학회지
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• 제42권1호
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• pp.9-15
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• 2009

A necessary condition for the formation of a filament is magnetic helicity. In the present paper we seek the origin of magnetic helicity of intermediate filaments. We observed the formation of a sinistral filament at the boundary of a decaying active region using full-disk $H_{\alpha}$ images obtained from Bi Bear Solar Observatory. We have measured the rate of helicity injection during the formation of the filament using full-disk 96 minute-cadence magnetograms taken by SOHO MDI. As a result we found that 1) no significant helicity was injected around the region (polarity inversion line; PIL) of filament formation and 2) negative helicity was injected in the decaying active region. The negative sign of the injected helicity was opposite to that of the filament helicity. On the other hand, at earlier times when the associated active region emerged and grew, positive helicity was intensively injected. Our results suggest that the magnetic helicity of the intermediate filament may have originated from the helicity accumulated during the period of the growth of its associated active region.

• 천문학회보
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• 제35권2호
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• pp.49.2-49.2
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• 2010

In order to understand the configuration of magnetic field producing a solar penumbral microjet that was recently discovered by Hinode, we performed a magnetohydrodynamic simulation reproducing a dynamic process of how that configuration is formed in a modeled solar penumbral region. A horizontal magnetic flux tube representing a penumbral filament is placed in a stratified atmosphere containing the background magnetic field that is directed in a relatively vertical direction. Between the flux tube and the background field there forms the intermediate region in which the magnetic field has a transitional configuration, and the simulation shows that in the intermediate region magnetic reconnection occurs to produce a clear jet- like structure as suggested by observations. The result that a continuous distribution of magnetic field in three-dimensional space gives birth to the intermediate region producing a jet presents a new view about the mechanism of a penumbral microjet, compared to a simplistic view that two field lines, one of which represents a penumbral filament and the other the background field, interact together to produce a jet. We also discuss the role of the intermediate region in protecting the structure of a penumbral filament subject to microjets.

• Preventive Nutrition and Food Science
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• 제3권1호
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• pp.92-97
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• 1998

The changes of morphology and protein pattern of sarcoma 180 cells treated with or without trannins extracted from persimmon leaves were evaluated by light microscopy, electrophoresis and Western blotting. The sarcoma 180 cells treated with tannins increased the amount of proteins which presumably were intermediate filament cytokeratins detected by electrophoresis and Western blot. Tannins was indirectly cytotoxic to the sarcoma 180 cells and increased the intermediate filament protein level in the cells.

• 대한의생명과학회지
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• 제12권4호
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• pp.413-418
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• 2006

Charcot-Marie-Tooth disease (CMT) is blown as one of the inherited disorder of peripheral nervous system. Recently, it was found that point mutations in the neurofilament light subunit (NF-L) gene cause CMT. Neurofilaments (NFs) are heteropolymers consist of NF-L, NF-M and NF-H. To assess the relationship between CMT and NF-L mutation in cellular level, we performed phenotypic analysis of the mutant NF-L (E397K) using cultured cell lines. Vimentin-deficient human adrenal carcinoma SW13 (Vim-) cells have a potential to form the intermediate filaments when the cells are expressing both NF-L and NF-M. Our results show that co-expression of wild type NF-L with NF-M showed intermediate filament formation in SW13 (Vim-) cells, while E397K with NF-M did not. This result means that E397K mutant lost its ability to form the intermediate filament in vivo, and further suggests that the E397K mutation is closely related to CMT.

• BMB Reports
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• 제41권12호
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• pp.868-874
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• 2008

Neurofilaments (NFs) are neuronal intermediate filaments composed of light (NF-L), middle (NF-M), and heavy (NF-H) subunits. NF-L self-assembles into a "core" filament with which NF-M or NF-H co-assembles to form the neuronal intermediate filament. Recent reports show that point mutations of the NF-L gene result in Charcot-Marie-Tooth disease (CMT). However, the most recently described rod domain mutant of human NF-L (A148V) has not been characterized in cellular level. We cloned human NF-L and used it to engineer the A148V. In phenotypic analysis using SW13 cells, A148V mutation completely abolished filament formation despite of presence of NF-M. Moreover, A148V mutation reduced the levels of in vitro self-assembly using GST-NF-L (H/R) fusion protein whereas control (A296T) mutant did not affect the filament formation. These results suggest that alanine at position 148 is essentially required for NF-L self-assembly leading to subsequent filament formation in neuronal cells.

• 한국잠사곤충학회지
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• 제40권2호
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• pp.150-157
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• 1998

This study was carried out to define the effects of anti-juvenile hormone analogue(AJH) treatment at different silkworm larval stages on some silk filament properties. It was revealed that the treatments at the 1st and 2nd days of the 3rd instar as well as the 1st day of the 4th instar resulted to trimolters induction without lethal effect. The trimolters induced by treatment at the 1st day of the 4th instar showed the shortest larval duration and the lowest single cocoon weight, cocoon shell ratio, cocoon size, single filament length and denier of filament compared with those of the control, while the trimolters induced by the 3rd-instar treatment showed intermediate values. All treatments showed no significant differences from the aspect fo degumming ratio, elongation rate and crystallinity index of fibroin. However the silk filament obtained from the treatment at the 1st day of the 4th instar showed the highest tenacity which was revealed to be related to the better uniformity of filament by the means of microscopic photographing. FT-IR spectroscopy of the silk fibroin showed a sharp and strong peak at the position of 794.72 cm-1 in all AJH-treated samples regardless of trimolters induction, which was absent in control.

• Advances in concrete construction
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• 제15권3호
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• pp.171-178
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• 2023

Cytoskeleton components play key role in maintaining cell structure and in giving shape to the cell. These components include microtubules, microfilaments and intermediate filaments. Among these filaments intermediate filaments are the most rigid and bear large compressive force. Actually, these filaments are surrounded by other filaments like microtubules and microfilaments. This network of filaments makes a layer as a surface on intermediate filaments that have great impact on buckling behavior of intermediate filaments. In the present article, buckling behavior of intermediate filaments is studied by taking into account the effects of surface by using Euler Bernoulli and Timoshenko beam theories. It is found that effects of surface greatly affect the critical buckling force of intermediate filaments. Further, it is observed that the critical buckling force is inversely proportional to the length of filament. Such types of observations are helpful for further analysis of nanofibrous in their actual environments within the cell.

• 한국추진공학회지
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• 제21권2호
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• pp.94-101
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• 2017

토우프리프레그는 한 가닥의 탄소섬유, 유리섬유, 아라미드 등의 보강섬유에 일정한 비율의 수지가 미리 함침되어 있는 토우 형태의 프리프레그를 의미하며, 필라멘트와인딩 공법을 위한 중간재이다. 최근 고성능 압력용기 개발에 대한 연구가 활발해지면서 기존의 습식 필라멘트와인딩 공법을 대체할 수 있는 건식 와인딩 공법을 위한 소재인 토우프리프레그에 대한 관심이 증대되고 있다. 본 논문에서는 로켓 모터케이스, 수소연료전지 자동차용 연료탱크, 산업용 고성능 압력용기 등 성능과 신뢰성을 동시에 필요로 하는 부품 제조용 소재로 주목받고 있는 탄소섬유 토우프리프레그의 국내외 최신 연구동향에 대하여 살펴보았다.

• The Plant Pathology Journal
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• 제29권1호
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• pp.17-30
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• 2013

Barley stripe mosaic virus (BSMV) induces massive actin filament thickening at the infection front of infected Nicotiana benthamiana leaves. To determine the mechanisms leading to actin remodeling, fluorescent protein fusions of the BSMV triple gene block (TGB) proteins were coexpressed in cells with the actin marker DsRed: Talin. TGB ectopic expression experiments revealed that TGB3 is a major elicitor of filament thickening, that TGB2 resulted in formation of intermediate DsRed:Talin filaments, and that TGB1 alone had no obvious effects on actin filament structure. Latrunculin B (LatB) treat-ments retarded BSMV cell-to-cell movement, disrupted actin filament organization, and dramatically decreased the proportion of paired TGB3 foci appearing at the cell wall (CW). BSMV infection of transgenic plants tagged with GFP-KDEL exhibited membrane proliferation and vesicle formation that were especially evident around the nucleus. Similar membrane proliferation occurred in plants expressing TGB2 and/or TGB3, and DsRed: Talin fluorescence in these plants colocalized with the ER vesicles. TGB3 also associated with the Golgi apparatus and overlapped with cortical vesicles appearing at the cell periphery. Brefeldin A treatments disrupted Golgi and also altered vesicles at the CW, but failed to interfere with TGB CW localization. Our results indicate that actin cytoskeleton interactions are important in BSMV cell-to-cell movement and for CW localization of TGB3.