• Journal of Ginseng Research
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• v.47 no.5
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• pp.662-671
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• 2023

Background: 20(S)-protopanaxadiol (PPD), a ginsenoside metabolite, has prominent benefits for the central nervous system, especially in improving learning and memory. However, its transcriptional targets in brain tissue remain unknown. Methods: In this study, we first used mass spectrometry-based drug affinity responsive target stability (DARTS) to identify the potential proteins of ginsenosides and intersected them with the transcription factor library. Second, the transcription factor PURA was confirmed as a target of PPD by biolayer interferometry (BLI) and molecular docking. Next, the effect of PPD on the transcriptional levels of target genes of PURA in brain tissues was determined by qRT-PCR. Finally, bioinformatics analysis was used to analyze the potential biological features of these target proteins. Results: The results showed three overlapping transcription factors between the proteomics of DARTS and transcription factor library. BLI analysis further showed that PPD had a higher direct interaction with PURA than parent ginsenosides. Subsequently, BLI kinetic analysis, molecular docking, and mutations in key amino acids of PURA indicated that PPD specifically bound to PURA. The results of qRT-PCR showed that PPD could increase the transcription levels of PURA target genes in brain. Finally, bioinformatics analysis showed that these target proteins were involved in learning and memory function. Conclusion: The above-mentioned findings indicate that PURA is a transcription target of PPD in brain, and PPD upregulate the transcription levels of target genes related to cognitive dysfunction by binding PURA, which could provide a chemical and biological basis for the study of treating cognitive impairment by targeting PURA.

• Journal of Digital Convergence
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• v.10 no.8
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• pp.135-148
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• 2012

This work is intended to investigate how the factors of enterprise mobile SNS affect user satisfaction and continuous use intention through technology acceptance model proposed by Davis. To achieve the purpose, this researcher explored Information Systems Success model proposed by DeLone & McLean, Technology Acceptance Model proposed by Davis, and Model after Acceptance, and on the basis of the investigation, performed a study. For the data of this work, 9 enterprises, each of which has more than 100 employees and is located in Seoul, were chosen, and a questionnaire survey was conducted on their 276 employees who experienced enterprise mobile SNS. As a data collection tool, a structured self-administered questionnaire was used. For data analysis, SPSS 18.0 and AMOS 18.0 were used for applying Structural Equation modelling. According to the results of this work, three factors of enterprise mobile SNS-systematic factor (system quality, information quality, and service quality), user factor (personal innovation and personal familiarity), social factor (social effects and social interaction)-affected user satisfaction and continuous use intention through perceived availability, perceived easiness, and perceived enjoyment. Also, it was found that the direction of effects matched a theoretical prediction. And, it was revealed that the decision variables and mediating variables significantly affected user satisfaction and continuous use intention. Theoretical and practical meanings were discussed for the study result, and some suggestions were made for the issues of this work and future studies.

• Journal of Internet Computing and Services
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• v.10 no.6
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• pp.51-79
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• 2009

Today the service industry is growing, and differentiated services for marketing and service quality is emerging as a serious problem. Until now, the WebCam is used for observation, and the negative aspects, such as from a human rights issue has become. Discipline and Punish is not to use a webcam to customer satisfaction research is needed. Therefore, wireless monitoring in WebCam service quality of the service reconfiguration and service quality factors for the configuration of the customer's preference regarding how research was recognized. Configuration of the service quality factors WebCam service customer satisfaction and customer loyalty was to identify factors that affect. These services to study the correlation between the quality variables WebCam provides wireless monitoring services for the preschool and children, a survey of the house. Configuring a wireless monitoring service quality in order to research the factors WebCam video quality and transmission speed, mobility and portability, convenience, visibility, reliability, and the interaction of the castle set. Severely and video quality and transmission speed, visibility, reliability, customer satisfaction, the interaction factors are said. Inclination to participate in social issues, innovation, digital devices that use a skilled, depending on the difference in the service quality has been confirmed. However, the age and quality of service awareness and job types showed a low correlation, and the marketing of these results will be discussed and how that can be used.

• Journal of Ginseng Research
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• v.45 no.4
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• pp.465-472
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• 2021

Background: Ginseng can help regulate brain excitability, promote learning and memory, and resist cerebral ischemia in the central nervous system. Ginsenosides are the major effective compounds of Ginseng, but their protein targets in the brain have not been determined. Methods: We screened proteins that interact with the main components of ginseng (ginsenosides) by affinity chromatography and identified the 14-3-3 ζ protein as a potential target of ginsenosides in brain tissues. Results: Biolayer interferometry (BLI) analysis showed that 20(S)-protopanaxadiol (PPD), a ginseng saponin metabolite, exhibited the highest direct interaction to the 14-3-3 ζ protein. Subsequently, BLI kinetics analysis and isothermal titration calorimetry (ITC) assay showed that PPD specifically bound to the 14-3-3 ζ protein. The cocrystal structure of the 14-3-3 ζ protein-PPD complex showed that the main interactions occurred between the residues R56, R127, and Y128 of the 14-3-3 ζ protein and a portion of PPD. Moreover, mutating any of the above residues resulted in a significant decrease of affinity between PPD and the 14-3-3 ζ protein. Conclusion: Our results indicate the 14-3-3 ζ protein is the target of PPD, a ginsenoside metabolite. Crystallographic and mutagenesis studies suggest a direct interaction between PPD and the 14-3-3 ζ protein. This finding can help in the development of small-molecular compounds that bind to the 14-3-3 ζ protein on the basis of the structure of dammarane-type triterpenoid.

• Journal of Digital Convergence
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• v.6 no.3
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• pp.45-52
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• 2008

For the domestic digital contents, it is particularly important that the planning and mediating functions should cover the whole Industries so that dividends and assistance to reach all the way back to the primary contents-creating level, where economic risks are especially high. A proactive cooperation system or framework between the government, the businesses and research institutions, which would allow for free flow of interaction among these players, can play that role. We can broadly define cooperation frameworks into two; one between the central and the local governments and another among private-sector groups, such as companies, associations and groups. These players in the framework have differing, sometimes, conflicting views regarding the digital contents industry whether the digital contents should aim for skilled applicability or the abstract or whether the emphasis should rest on public interest or profitability. Immature competition and lack of trust among these players also give rise to such inefficiencies as overlap in investments, inexpertness and inefficient use of resources. We have proposed and realized the National Integrated Cooperation System to bridge these gaps among the major players in the industry. The National Integrated Cooperation System rests on the following major functions. The first major function of the NICS is to decipher any ambiguity that may be embedded in external Inputs by stratified role and bias. The second function is to create cooperative groups that will deal with the ambiguities based on its consequent situation. The third is a feedback function that will draw out a new cooperative way by re-feeding the capacities and the conflicts that stem from the existing organizations and strategies into cooperation and adjustment process. Our NICS has compared and evaluated with England and Australia digital content industry models under AHP(Analytic Hierarchy Process) method. NICS has turned out to be well designed and have strong points based on OECD innovation and cooperation criteria.

• Journal of Digital Convergence
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• v.11 no.8
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• pp.95-108
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• 2013

Agile development methods regards an interaction among software developers, operating softwares, and cooperation with customers as its main value. Most of the developing processes undergo a procedure called tailoring to meet the relevant project. Yet, in reality, projects run in disorder due to their overlap with other methods and management processes. As a result, the advantages of agile process cannot be fully used. So, the information system audit has an important role to detect these problems and improve the qualities of agile application projects. Also, researches should be conducted on Agile-based Iformation System Audit Model in order to enhance the effectiveness of agile projects. This paper suggests a model for an information system audit that applied agile developing methods. This model proposes audit domains and check lists for agile-based information system. In order to verify the suitability of the suggested model, more than 89.3% of the checklists were verified as suitable in all domains.

• Journal of Information Management
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• v.36 no.4
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• pp.199-215
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• 2005

Experts participating in the knowledge expert network externalize their implicit knowledge by providing information or writing reports. Almost all the members of the network share externalized knowledge and the network facilitate the dissemination and diffusion of knowledge. Individuals reproduce another implicit knowledge by internalizing shared knowledge through the network and re-created knowledge is externalized, establishing knowledge circulation. In this paper, we analyze the expert groups of the Global Network of Korean Scientists & Engineers(KOSEN, www.kosen21.org), the Korea's No. 1 science and engineering knowledge expert community, with the application of the theory of policy network proposed by Marsh & Rhodes. According to the principal standards of policy network classification such as the number of participants, interaction among participants, consistency, distribution of resources and dependency, we categorize the KOSEN expert groups as closed policy network and opened issue network, and divide closed policy network into core community and periphery community.

• Journal of Society of Preventive Korean Medicine
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• v.14 no.2
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• pp.1-12
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• 2010

The individual differences in disease development and susceptibility have been researched primarily on the subject of genes, environment or the interaction between genes and the environment respectively. However, there have been limitations in explaining complex diseases, and the differences in health and diseases in monozygotic and dizygotic twins. Fortunately, thanks to active research on the relationship between genes and the environment, and epigenetics, there has been much progress in the understanding of body's reactions and changes. Epigenetics is referred to as a study of gene expression through the interactions of DNA methylation, chromatin's histone and the change of structure in tail, RNA editing without any change in DNA sequence. In this paper, we introduce the basic concepts and mechanisms of epigenetics. The result of the epigenetics is heritable ; can regulate gene expressions ; is reversible ; and has many variable forms depending on cell types. The influences of epigenetics occur throughout life, but it is mainly determined in utero during early pregnancies. Diseases occur or the risk rises if these influences continue after birth until adult life when problems occur in excess/lack of nutrition, environmental plasticity, or already inputted data. Therefore, there is a need for change and innovation, especially in interest and investment in health education for young women near pregnancies and correct treatment of epigenetic-related diseases.

• Journal of the Korean Society of Costume
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• v.58 no.4
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• pp.113-127
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• 2008

Artificial lights have effected the changes of art and fashion concepts as well as human life since the invention of electric light bulb in late 19th century. Artist and designer have had more interested in these artificial lights as the development of digital technology and the change of millennium and they have tried to apply the lights into their works. The purpose of this study is to analyze the aesthetic characteristics of contemporary fashion design using artificial light as a medium. Artificial light for fashion design means the light using luminescent material like phosphorescent and fluorescent materials or in combination with electroluminescent digital technology or the light that can be perceived as images when light projects from media using a light projector or other digital equipment. Fashion design using this light type can change colors or form temporarily and it can playa role as a gadget for hm or as equipment to provide information much as a computer monitor does. And designer can create virtual patterns on the surface of clothes, or virtual fashion like a 3-dimensional holography in empty space. In these fashion designs, the virtual image of light is substituted for physical formative elements in fashion, and the viewer can experience an ambiguity between reality and virtuality. The results of the study were as follow; The formative characteristics of those fashion designs were identified as visibility, indeterminacy, integration and virtuality. And they reflected the internal meanings; the persue of protection and safety, the search for experiment and innovation, the will for interaction and communication and the desire for the deviation and fun.

• Journal of Satellite, Information and Communications
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• v.12 no.4
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• pp.11-17
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• 2017

In this paper, we propose the authoring tools engine supported a 4 dimension display contents which serve display of high quality (UHD*250) curved using 4 dimension engine, which is a innovation technology by authoring tools of management software supported 4 dimension editing environment on curved flexible display. The important technology is as follows, the first, the authoring tools of 4 dimension viedo wall for a flexible high quality display(UHD*256) using multiscreen synchornization, the second implemented a bidirection interaction technology of UI/UX interface, which is established by 360 degree recognition sensor for digital signage, the 4 dimension viedowall flexible display with curve, the third, implemented the authoring tool software for various dimension electronic books first in the world.