• Title/Summary/Keyword: Intensity Analysis

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Intensity measure-based probabilistic seismic evaluation and vulnerability assessment of ageing bridges

  • Yazdani, Mahdi;Jahangiri, Vahid
    • Earthquakes and Structures
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    • v.19 no.5
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    • pp.379-393
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    • 2020
  • The purpose of this study is to first evaluate the seismic behavior of ageing arch bridges by using the Intensity Measure - based demand and DCFD format, which is referred to as the fragility-hazard format. Then, an investigation is performed for their seismic vulnerability. Analytical models are created for bridges concerning different features and these models are subjected to Incremental Dynamic Analysis (IDA) analysis using a set of 22 earthquake records. The hazard curve and results of IDA analysis are employed to evaluate the return period of exceeding the limit states in the IM-based probabilistic performance-based context. Subsequently, the fragility-hazard format is used to assess factored demand, factored capacity, and the ratio of the factored demand to the factored capacity of the models with respect to different performance objectives. Finally, the vulnerability curves are obtained for the investigated bridges in terms of the loss ratio. The results revealed that decreasing the span length of the unreinforced arch bridges leads to the increase in the return period of exceeding various limit states and factored capacity and decrease in the displacement demand, the probability of failure, the factored demand, as well as the factored demand to factored capacity ratios, loss ratio, and seismic vulnerability. Finally, it is derived that the probability of the need for rehabilitation increases by an increase in the span length of the models.

Optimization of Gene Transfection Using Fluorescence-Activated Cell Sorter(FACS) Analysis of Green Fluorescent Protein(GFP) (Green Fluorescent Protein(GFP)의 Fluorescence-Activated Cell Sorter(FACS) 분석을 통한 유전자 이입의 최적화)

  • 김태경;박민태;이균민
    • KSBB Journal
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    • v.14 no.3
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    • pp.377-379
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    • 1999
  • In order to improve the transfection efficiency of CHO/dhfr- cells using cationic lipid, optimal concentrations of the cationic lipid($LipofectAmine^{TM}$) and DNA(pEGFP-C1) need to be determined. The use of green fluorescent protein(GFP) gene as a reporter gene facilitated the quantification of transfection efficiency. The green fluorescence intensity of each cell transfected at various lipid-DNA concentrations was measured using fluorescence-activated cell sorter(FACS) analysis. A combination of $2.0{\mu}L$ cationic lipid and 0.4{$\mu}g$ DNA in a well resulted in the highest trasfection efficiency. Taken together, the method using FACS analysis of GFP is simple and fast, facilitating the optimization of transfection.

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Analysis of Three-Dimensional Cracks in Inhomogeneous Materials Using Fuzzy Theory

  • Lee, Yang-Chang;Lee, Joon-Seong
    • International Journal of Fuzzy Logic and Intelligent Systems
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    • v.5 no.2
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    • pp.119-123
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    • 2005
  • This paper describes a fuzzy-based system for analyzing the stress intensity factors (SIFs) of three-dimensional (3D) cracks. 3D finite element method(FEM) was used to obtain the SIF for subsurface cracks and surface cracks existing in inhomogeneous materials. A geometry model, i.e. a solid containing one or several 3D cracks is defined. Several distributions of local node density are chosen, and then automatically superposed on one another over the geometry model by using the fuzzy theory. Nodes are generated by the bucketing method, and ten-noded quadratic tetrahedral solid elements are generated by the Delaunay triangulation techniques. The singular elements such that the mid-point nodes near crack front are shifted at the quarter-points, and these are automatically placed along the 3D crack front. The complete FE model is generated, and a stress analysis is performed. The SIFs are calculated using the displacement extrapolation method. The results were compared with those surface cracks in homogeneous materials. Also, this system is applied to analyze cladding effect of surface cracks in inhomogeneous materials.

Immunohistochemical analysis of the effect of low power GaAlAs laser treatment on the expression of proliferating cell nuclear antigen (PCNA) in full-thickness excisional wound of rat skin (CaAlAs 저출력 레이저 자극이 흰쥐의 피부 전층결손 절제 창상의 치유시 proliferating cell nuclear antigen(PCNA)발현에 대한 면역조직화학법적 분석)

  • Kim, Soon-Ja;Koo, Hee-Seo
    • Journal of Korean Physical Therapy Science
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    • v.10 no.1
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    • pp.198-205
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    • 2003
  • We evaluated the effect of low power GaAsAl laser on re-epithelization in full-thickness excisional wound of rat skin. Two full-thickness excisions were made on the back of the experimental animals. Low power laser applications with 10mW intensity were treated experimental animals twice a day for 7 days. On the seventh postoperative day the quantitative analysis of re-epithelization was performed using immunohistochemical staining for proliferating cell nuclear antigen (PCNA). The majority of PCNA immunoreactive cells was observed at epithelial cells in the margin of full thickness excisional wound. The low power laser treatments significantly increased the number of PCNA immunoreactive cell as compared to that of non treated animal group (p<0.01). The shape of PCNA immunoreactive cell appeared as small dark, round to ovoid structures. Most PCNA immunoreactive cells exhibited a high intensity of staining that contrasted sharply with the surrounding background. In conclusion, these findings suggest that GaAlAs laser treatments effectively enhance the epithelial wound healing by the stimulating cell proliferation. Furthermore, the majority of cell proliferation occurred in the margin of full thickness excisional wound.

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Sensory Profiles of Cooked Rice, Including Functional Rice and Ready-to-Eat Rice by Descriptive Analysis (묘사분석에 의한 기능성쌀밥 및 즉석밥의 관능프로필)

  • Kim, Dong-Hee;Kim, Hee-Sup
    • Korean journal of food and cookery science
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    • v.23 no.5
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    • pp.761-769
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    • 2007
  • The purpose of this study was to provide the sensory profiles for cooked rice varieties, with 3 types of functional rice and 4 types of ready -to -eat rice, using descriptive analysis. Eighteen descriptors as well as reference standards were developed. The cooked functional rice, ready-to-eat rice, and plain cooked rice were separated into 4 groups by PCA. PC1 & PC2 explained 65% of the total valiance. PC 1 was responsible for the highest percentage of separation based on the texture intensities. The cooked functional rice showed higher intensity for hardness and roughness. The ready-to-eat rice A, B, and C samples had higher intensities in terms of moistness, glossiness, and moisture content, as well as sour and raw rice flavor. The ready-to-eat rice D sample had low grain wholesomeness, and high intensity for raw rice flavor. Finally, the plain cooked rice had more roasted and burnt rice flavor.

Analysis of crack occurs under unsteady pressure and temperature in a natural gas facility by applying FGM

  • Eltaher, Mohamed A.;Attia, Mohamed A.;Soliman, Ahmed E.;Alshorbagy, Amal E.
    • Structural Engineering and Mechanics
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    • v.66 no.1
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    • pp.97-111
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    • 2018
  • Cracking can lead to unexpected sudden failure of normally ductile metals subjected to a tensile stress, especially at elevated temperature. This article is raised to study the application of a composite material instead of the traditional carbon steel material used in the natural gas transmission pipeline because the cracks occurs in the pipeline initiate at its internal surface which is subjected to internal high fluctuated pressure and unsteady temperature according to actual operation conditions. Functionally graded material (FGM) is proposed to benefit from the ceramics durability and its surface hardness against erosion. FGM properties are graded at the radial direction. Finite element method (FEM) is applied and solved by ABAQUS software including FORTRAN subroutines adapted for this case of study. The stress intensity factor (SIF), temperatures and stresses are discussed to obtain the optimum FGM configuration under the actual conditions of pressure and temperature. Thermoelastic analysis of a plane strain model is adopted to study SIF and material response at various crack depths.

A Study of Musculoskeletal Disorders at Automobile Industries in Korea (국내 자동차산업의 근골격계질환 실태에 관한 연구)

  • 김철홍
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.24 no.67
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    • pp.1-10
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    • 2001
  • A series of studies with collaboration of university, research institute, and labor union were conducted to examine the situation and severity of work-related musculoskeletal disorders (MSDs) at an automobile company which is one of the three major car companies in Korea. As the first part of the study, a questionnaire survey was conducted on 4,000 employees at 2 different assembly sites and A/S centers over the country. The questionnaire was consisted of three parts; symptom survey, work environment information, and labor intensity survey. Among 4,000 questionnaires, 2,643 responses were considered for analysis. The result showed that 68.5% of the respondents complained pains on at least one part of the body and 48.8% on at least two parts of the body. After cross-sectional analysis of various information and risk factors such as severity of symptom, duration and frequency of pain, 31.5% of respondents were considered as at high risk of having MSDs. This high-risk rate showed similar result when compared with previous studies conducted at various manufacturing industries in Korea. Also the analysis of the work environment and job intensity survey supported the high-risk rate of MSDs.

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An Application of 2-D BEM with Laplace Transformation to Impact Crack Analysis (균열의 충격해석에 대한 Laplace 변환 2차원 경계요소법의 응용)

  • 조상봉;김태규;최선호
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.16 no.5
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    • pp.883-890
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    • 1992
  • Analysis of dynamic or impact problems is very important in engineering fields such as airplanes and automobiles. In the present study, two-dimensional elastodynamic BEM program with Laplace transformation is developed to analyze dynamic or impact problems. Accuracy and efficiency of the BEM program are tested by making the comparision of impact analysis of some models with other's published results. The BEM developed is applied to the impact crack problem and the dynamic stress intensity factors of some impact cracks is obtained by the displacement extrapolation method. It is confirmed to be possible to analyze impact problems accurately with only a little elements in simple models. And also it is found to be careful to use the singular element usually using in static crack problems because that the elastodynamic fundamental solution usually using in static crack problems because that the elastodynamic fundamental solution has more sensitive singularity than the static fundamental solution and to determine the boundary conditions in dynamic problems.

Comparative Analysis for General and Estrus-related Vocalizations in Sows (모돈의 일반 발성음과 발정기 특이음의 비교분석)

  • Jeon, J.H.;Yeon, S.C.;Chang, H.H.
    • Journal of Animal Science and Technology
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    • v.47 no.1
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    • pp.133-140
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    • 2005
  • The aim of this study was to divide vocalizations of sows into general(GVs) and estrus-related vocalizations( EVs) and to find out their phonetic characteristics. Ten sows(Landrace) were recorded using digital video recorders twice daily(06: 00 - 08 : 00h and 17: 00 - 19 : 00h) during the anestrus and estrus periods. The GVs and EVs were divided based on the shapes of spectrum and spectrogram. The GVs and EVs were identified as 5 and 3 types, respectively. Pitch, formant I, formant 2, and formant 3 between GVs and EVs were not significantly different(P> 0.05), whereas intensity(P < 0.001), duration(P < 0.05), and formant 4(P < 0.01) were significantly different. Three parameter groups(Group I : Formant vector alone, Group II: Formant veetor+ parameters from time signal, Group III: Formant vector+parameters from time signal-parameters eliminated by stepwise discriminant analysis backward) were compared by discriminant function analysis. The classification system adopted in the Group II represented the higher discrimination rate than those in other groups(Group I : 76.1 0/0, Group II : 88.1 0/0, Group Ill: 87.3 %). These results suggest that EVs are present and intensity, formant 2, and formant 4 are available parameters for discrimination of EVs in sows.

Fracture Analysis of Spot-Welds with Edge Cracks using 2-D Hybrid Special Finite Element (이차원 하이브리드 요소를 이용한 균열을 내포하는 용접점의 유한요소 파단해석)

  • Song J. H.;Yang C. H.;Huh H.;Kim H. G.;Park S. H.
    • Transactions of Materials Processing
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    • v.13 no.6 s.70
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    • pp.484-489
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    • 2004
  • This paper employed a systematic analysis using a 2-D hybrid special finite element containing an edge crack in order to describe the fracture behavior of spot-welds in automotive structures. The 2-D hybrid special finite element is derived form a mixed formulation with a complex potential function with the description of the singularity of a stress field. The hybrid special finite element containing an edge crack can give a better description of its singularity with only one hybrid element surrounding one crack. The advantage of this special element is that it can greatly simplify the numerical modeling of the spot welds. Some numerical examples demonstrate the validity and versatility of the present analysis method. The lap-shear, lap-tension and angle-clip specimens are analyzed and some useful fracture parameters such as the stress intensity factor and the initial direction of crack growth are obtained simultaneously.