• Journal of The Korean Society of Agricultural Engineers
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• v.48 no.2
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• pp.3-9
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• 2006

The chemical fractions of phosphorus were investigated in the soils of reclaimed and natural tidelands of southwest coastal area of Korea. The content of total-P varied to ranged from 322 to 614 mg/kg dry weight with a mean value of 467 mg/kg. The order of different fractions of phosphorus in reclaimed tideland soils was as follows: 1) inorganic phosphorus: Ca-P > Fe-P > Al-P > reductant soluble Fe-P. 2) organic phosphorus: Inositol-P > nucleic acid-P > phospholipid-P. The content of Al-P showed negative correlation with organic matter content but showed positive correlation with pH. The content of Fe-P showed negative correlation with clay mineral content but showed positive correlation with organic matter content. Reductant soluble Fe-P and Ca-P were no correlation with soil properties. The content of inositol-P showed highly positive correlation with clay mineral, organic matter content, and CEC.

• Journal of Photoscience
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• v.6 no.2
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• pp.71-75
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• 1999

Tempting to further understand the molecular mechanism of pharmacological action of ethanol, we evaluated effects of phosphatidylethanol (PET) on inositol 1,4,5-triphosphate (IP3) level and protein kinase C (PKC) activity in cultured NG108-15 cells. PET increased intracellular concentration of IP$_3$. PET incorporation into membranes of NG108-15 cells had no effect on the phosphorylation of the PKC-specific substrate MBP$\_$4-14/, thus indicates that PET does not affect PKC activity in this system.

• Korean Journal of Microbiology
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• v.40 no.3
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• pp.211-216
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• 2004

A bacterial strain JE-ll found to produce active extracellular phospholipase D (PLD) was selected from the soil isolates. It was identified as Streptomyces somaliensis on the basis of 16S rDNA sequence analysis, morphological and physiological characteristics. The gene (sspld) encoding S. somaliensis PLD was isolated and characterized. The open reading frame was suggested to encode 538 amino acids with a signal peptide of 33 amino acids. The deduced amino acid sequence of the sspld shared a sequence similarity of 70-88％ with PLDs of other Streptomyces sp. so far reported. The PLD converted phosphatidylcholine to phosphatidylglycerol or phosphatidylserine with the yield of 96 to 99％ (㏖/㏖), but did not act on inositol or ethanolamine as a transphosphatidylation donor.

• Journal of Applied Biological Chemistry
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• v.62 no.4
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• pp.425-431
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• 2019

Platelet activation is essential for hemostatic process on blood vessel damage. However, excessive platelet activation can cause some cardiovascular diseases including atherosclerosis, thrombosis, and myocardial infarction. Scoparone is commonly encountered in the roots of genus Artemisia or Scopolia, and has been studied for its potential pharmacological properties including immunosuppression and vasorelaxation, but antiplatelet effects of scoparone have not been reported yet. We investigated the effect of scoparone on human platelet activation prompted by an analogue of thromboxane A₂, U46619. As the results, scoparone dose-dependently increased cyclic adenosine monophosphate (cAMP) levels as well as cyclic guanosine monophosphate (cGMP) levels, both being aggregation-inhibiting molecules. In addition, scoparone strongly phosphorylated inositol 1, 4, 5-triphosphate receptor (IP3R) and vasodilator-stimulated phosphoprotein (VASP), substrates of cAMP dependent kinase and cGMP dependent kinase. Phosphorylation of IP₃R by scoparone resulted in inhibition of Ca²⁺ mobilization in calcium channels in a dense tubular system, and phosphorylation of VASP by scoparone led to an inability of fibrinogen being able to bind to αIIb/β3. Finally, scoparone inhibited thrombin-induced fibrin clotting, thereby reducing thrombus formation. Therefore, we suggest that scoparone has a strong antiplatelet effect and is highly probable to prevent platelet-derived vascular disease.

• Archives of Pharmacal Research
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• v.27 no.12
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• pp.1207-1210
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• 2004

The antidiabetic activity-guided fractionation and isolation of the 80% EtOH extracts from Peucedani Radix (Peucedanum japonicum, Umbelliferae) led to the isolation and characterization of a coumarin and a cyclitol as active principles, that is, peucedanol 7-O-${\beta}$ -D-glucopyranoside (1) and myo-inositol (2). Their structures were identified by spectroscopic methods. Compound 1 showed 39% inhibition of postprandial hyperglycemia at 5.8 mg/kg dose, and compound 2 also significantly inhibited postprandial hyperglycemia by 34% (P<0.05).

• Journal of Applied Biological Chemistry
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• v.63 no.3
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• pp.235-241
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• 2020

An essential component of the hemostatic process during vascular damage is platelet activation. However, many cardiovascular diseases, such as atherosclerosis, thrombosis, and myocardial infarction, can develop due to excessive platelet activation. Isoscopoletin, found primarily in plant roots of the genus Artemisia or Scopolia, has been studied to demonstrate potential pharmacological effects on Alzheimer's disease and anticancer, but its mechanisms and role in relation to thrombus formation and platelet aggregation have not yet been discovered. This research investigated the effect of isoscopoletin on collagen-induced human platelet activation. As a result, isoscopoletin strongly increased cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) levels in a concentration-dependent manner. In addition, isoscopoletin greatly phosphorylated inositol 1,4,5-triphosphate receptor (IP₃R) and vasodilator-stimulated phosphoprotein (VASP), known substrates of cAMP-dependent kinase and cGMP dependent kinase. Phosphorylation of IP₃R by isoscopoletin induced Ca²⁺ inhibition from the dense tubular system Ca²⁺ channels, and VASP phosphorylation was involved in fibrinogen binding inhibition by inactivating αIIb/β₃ in the platelet membrane. Isoscopoletin finally reduced thrombin-induced fibrin clot production and finally reduced thrombus formation. Therefore, this research suggests that isoscopoletin has strong antiplatelet effects and is likely to be helpful for thrombotic diseases involving platelets by acting as a prophylactic and therapeutic agent.

• Journal of the Korean Society of Food Science and Nutrition
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• v.25 no.5
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• pp.735-740
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• 1996

This study was carried out to determine the composition of glycolipids and phospholipids in Japonica and Indica rice bran oils. The ratio of glycolipids and phospholipids was 4.1 : 6.5% in Japonica rice bran oils and 2.6 : 3.7% in Indica rice bran oils. Polar lipid content was significantly higher in Japonica rice bran oils. The main components of glycolipids were esterified steryl glycoside, monogalactosyl diglyceride steryl glycoside, cerebroside and digalactosyl diglyceride. The content of esterified steryl glycoside was the highest, resulting in 48.8~52.1% of total glycolipids. Phospholipids in rice bran oils consisted of diphosphatidyl glycerol, phosphatidyl ethanolamines, phosphatidyl inositol, phosphatidyl serines, phosphatidyl choline and lysophosphotidyl choline. Major fatty acids of the glycolipids and phospholipids fractions were oleic, linoleic and palmitic acids in Japonica and Indica rice bran oils.

• Journal of Microbiology and Biotechnology
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• v.12 no.2
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• pp.279-285
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• 2002

Phytase (myo-inositol hexakisphosphate phospho-hydrolase, EC 3.1.3.8) catalyzes the hydrolysis of phytate (myo-inositol hexakisphosphate) to release inorganic phosphate. A bacterial strain producing phytase was isolated from soil around a cattle shed. To identify the strain, cellular fatty acids profiles, the GC contents, a quinine-type analysis, and physiological test using an API 20NE kit were carried out. The strain was identified to be a genus of Pseudomonas sp. and named as Pseudomonas sp. YH40. The optimum culture condition for the maximum productivity of phytase by Pseudomonas sp. YH40 were attained in a culture medium composed of $1.0\%$ (w/v) glycerol, $2.0\%$ (w/v) peptone, and $0.2\%$ (w/v) $FeSO_4{\cdot}7H_2O$. Within the optimal medium condition, the production of phytase became highest after 10 h of incubation, and the maximal phytase production by Pseudomonas sp. YH40 was observed at $37^{\circ}C$ and pH 6.0.

• Proceedings of the Korean Society of Life Science Conference
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• 2001.06a
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• pp.53-66
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• 2001

본 실험실에서는 혈당강하효과를 갖는 chiroinositol을 다량 함유하고 있는 식품이나 식용물질을 탐색하여 선발하고, 선택된 식품이나 식용물질에서 chiroinositol을 lab-scale로 분리 정제하여 제조된 chiroinositol의 추출물로 동물실험을 실시하여 혈당강하효과를 증명하고 그 기작을 연구하고 있다. 약 300여종의 식품 및 식용물질을 GC-MS 및 HPLC로 분석한 결과 식품으로 안전한 탈지대두와 두부 순물에서 chiroinositol의 함량이 각각 6.45mg/g, 20mg/g으로 조사되어 선택되었고, chiroinositol의 순도가 40-60%(w/w)인 chiroinositol의 추출물을 이용하여 동물실험을 통한 혈당강하효과를 조사한 결과, 1) steptozotocin(STZ)으로 유발된 고혈당쥐에 경구투여시, 농도 의존적으로 혈당을 감소시키는 탁월한 기능을 나타내었고, 2) 정상쥐의 혈당제거율 검사(glucose tolerance test)에서강한 제거율 및 6시간의 지속시간을 나타내었으며, 3) STZ로 유발된 고혈당쥐의 혈당제거율 검사에서 강한 혈당강하 효과(약 40%)를 나타내었으며 지속시간은 약 12시간이었다. 4) 극심한 고혈당(450 내지 500mg/d)의 경우, 경미한 고혈당(300내지 350mg/d)의 경우보다 혈당 감소율이 20% 가량 높았으며, 지속시간도 1시간 이상 긴 것으로 나타났으며, 5) 인슐린과 복합처리 했을 때 상승작용(synergy)을 나타내었으며, 저혈당증으로 전혀 발전되지 않았다.

• YAKHAK HOEJI
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• v.41 no.4
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• pp.433-438
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• 1997

MT 51005 as a potent inhibitor of phospholipase C(PLC) was purified from the culture broth of a fungal strain No. 51005 isolated from soil. It was identified as a benzaldehyde d erivative, anguillosporal. by the physico-chemical properties and spectroscopic data. Anguillosporal showed the inhibitory activity against purified PLC with an $IC_{50}\;of\;13{\mu}g/ml$. And it also inhibited the formation of inositol phosphates($IP_t$) in platelet-derived growth factor(PDGF)-stimulated $NIH3T3{\gamma}1$ cells with an $IC_{50}\;of\;0.8{\mu}g/ml$.