• 한국자원식물학회지
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• 제11권3호
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• pp.296-300
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• 1998

When medicinal mushroom, Poria cocos, is cultured , inoculation method of spawn is cross slice inoculation of which the both sides of pine tree were peeled and spawn of P.cocos was inoculated. However, this method required lots of inoculation amount. This study was carried out to improve the culturing method of P. cocos. A good growth of P.cocos was observed in MCM(mushroom complete medium), showing proper mycelia growth and density. In inoculation amount, conventional method(cross slice inoculation) requires 20 bottles of spawn. In contrast, short log method required 8 bottles of spawn and drilling inoculation method 2~3 bottles, which could save by 60% and 85-90% respectively. In the selectrion of tree species, pine and larch had better condition for spawn culture and sclerotia formation condition.In terms of yield , pine was 33.7kg/3.3$m^2$. In the yield of pine, conventional method was 23.4kg/3.3$m^2$, drilling inoculation 29.4kg/3.3$m^2$, short log inoculation 31.7kg/3.3$m^2$, therefore drilling inoculation could increase by 25% and short log inoculation 35%, In addition, management cost was also saved.

• International Journal of Industrial Entomology and Biomaterials
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• 제34권2호
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• pp.38-44
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• 2017

Mulberry leaves containing 1-deoxynojirimycin (DNJ) have been recognized as a potentially important source for prevent or treat hyperglycemia. However, DNJ content of natural mulberry leaf are as low as 0.1%. Thus, the most effective method for increasing ${\alpha}$-glucosidase inhibitory activity with the DNJ high-production is needed. In this study, we investigated the influence of ${\alpha}$-glucosidase inhibitory activity according to different pH values (6-9) and inoculation amounts (0.1-0.5%) when Bacillus subtilis cultured on mulberry leaf powder media. We confirmed that ${\alpha}$-glucosidase inhibitory activity was difference according to culture conditions of different pH values, inoculation amounts, and fermentation times. The results of mulberry leaf fermentation according to pH values and inoculation amounts were shown that the optimal conditions for ${\alpha}$-glucosidase inhibitory activity were defined as pH 7 and 9, inoculation amount 0.4%, and incubation until 2 to 4 days. These results can be provided a basic data for the optimal culture conditions increasing ${\alpha}$-glucosidase inhibitory activity from mulberry leaf fermentation.

• Journal of the Korean Wood Science and Technology
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• 제26권1호
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• pp.19-28
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• 1998

For cultivation on sawdust-bed of oak-mushroom until present time, inoculation of spawn on sawdust bed has been performed by sawdust spawn. But, liquid spawn may have advantages for rapid mass production of spawn, and now, sawdust-cultivation by liquid spawn inoculation should be applied instead of sawdust spawn. Therefore, investigations were performed to evaluate the effect of sawdust-cultivation by liquid spawn inoculation. The results were as follows: 1. When 11 kinds of liquid media were applied, the oak-mushroom culture medium was the most excellent in growth. Most suitable temperature at PDA was $25^{\circ}C$, and $22.5\sim27.5^{\circ}C$ in range were optimal for liquid culture. In liquid culture, amount of mycelial growth increases rapidly up to 40 days of cultivation. Incubation at fermentor brought yield of 106mg dry mycelia per 40ml media after 17 days. 2. In 1l-spawn bottle, growth of mycelium by inoculation of 20ml-liquid spawns were faster than 6g-sawdust spawn in spread of mycelia. On 2kg-bag culture, inoculations of 10ml-, 20ml- and 30ml-liquid spawns were all slower than 20g-sawdust spawn in mycelial spread. So, amount increasement in ampunt of liquid spawn should be discussed. Yields of mushrooms until third sproutings of 2kg-bag culture were 580g in 30ml-liquid spawn inoculation, but 510g, 486g and 470g from 20g-sawdust spawn, 20ml-liquid spawn and 10ml-liquid spawn, respectively. Thus, 30ml-liquid spawn inoculation was highest in yield.

• 한국미생물·생명공학회지
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• 제36권3호
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• pp.233-239
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• 2008

A new approach to the solubilization of excess activated sludge by the inoculation of lactic acid bacteria was studied to reduce the amount of sludge produced in the activated sludge treatment process. Aerobic microorganism in sludge was lysed in anaerobic condition and the cytoplasmic substance eluted was utilized as a carbon source by lactic acid bacteria. On the basis of sludge solubilization efficiency, Lactobacillus brevis and Leuconostoc mesenteroides subsp mesenteroides were selected the best candidates among five kinds of Lactobacillus sp. and seven kinds of Leuconostoc sp. The sludge solubilization efficiency by heterofermentative lactic acid bacteria was more efficient than that of homofermentative bacteria. Initial value of soluble COD (sCOD) was 1050 mg/L at the initial inoculation time increased to 3070 mg/L (192% solubilization) at 96 h of the incubation time. The inoculation of lactobacillus brevis to the sludge resulted in 2824% increase in sCOD value after 96 h of incubation than the control experiment. Leuconostoc mesenteroides subsp mesenteroides showed 152% increase of solubilization and 30% increase of S-COD/T-COD on 96 h of incubation time. Considering the increase of S-COD by the inoculation of Leuconostoc sp. on 24 h, 10% inoculation of lactic acid bacteria to the sludge was most effective.

• 한국식물병리학회지
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• 제13권2호
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• pp.85-89
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• 1997

Etiological components of the rice cultivars possessing durable resistance to rice blast were evaluated. In the greenhouse test by artificial inoculation, number of leaf blast lesion in Dongjinbyeo was not increased by any race of the pathogen, while the lesion number on the resistant cultivar Palgongbyeo and susceptible cultivar Jinmibyeo was increased rapidly. Size of the lesion in Dongjinbyeo was smaller and progressed more slowly than the resistant cultivar Palgongbyeo until 11 days after inoculation. Latent period in Dongjinbyeo was intermediate. However, the period was significantly variable depending upon the races. Least amount of conidia was produced in Dongjinbyeo by race KJ-107 and the number of conidia was decreased rapidly from 11 days after inoculation.

• 한국균학회지
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• 제49권4호
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• pp.525-530
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• 2021

The cultivation in sawdust media, mushroom productivity, and fruiting body characteristics of Lentinula edodes strains NIFoS 2778 and NIFoS 3363 were compared according to the inoculation conditions. The cultivation period was 5% shorter when liquid spawn was used. Fruiting bodies were induced after 113 days of incubation on media inoculated with liquid spawn, and the cultivation period was 119 days on media inoculated with solid spawn. Mushroom productivity of NIFoS 2778 was the highest (661.4 g) when 36 mL of liquid spawn was used. For NIFoS 3363, mushroom production was higher under liquid inoculation conditions when the same amount of liquid and solid spawns were used. The mushroom characteristics of the two strains were not significantly different, except for gill width and stipe diameter.

• 대한수의학회지
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• 제16권1호
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• pp.53-57
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• 1976

The growth of Sal. choleraesuis and its kunzendorf variety in tetrathionate broth containing various amounts of iodine solution was studied and compared with that of Sal. typhi, Sal. typhimurium and E. coli. The results obtained were as followings. 1. When 2.0 ml of iodine solution, normal amount, was added to 100 ml of tetrathionate broth base, the number of Sal. choleraesuis decreased rapidly until 24 hours after inoculation and slightly increased 48 hours after inoculation. The numbers of Sal. choleraesuis var. kunzendorf and E. coli decreased rapidly and none of the organisms recovered 24 and 48 hours after inoculation, respectively. The growth of Sal. typhi and Sal. typhimurium, however, was not inhibited at all. 2. When 4.0 ml of iodine solution was added, to 100 ml of tetrathionate broth base, the growth of all the organisms was inhibited, among which Sal. choleraesuis, Sal. choleraesuis var. kunzendorf, and E. coli were not recovered 24 and 48 hours after inoculation. 3. When reduced amounts of iodine solution, 1.0 ml and 0.5 ml, were added to 100 ml of tetrathionate broth base, the growth of all the organisms was not inhibited.

• 한국토양비료학회지
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• 제25권4호
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• pp.378-386
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• 1992

Cowpea군(群) 두과작물(豆科作物)에 대한 땅콩근류균(根瘤菌) HCR-46의 근류형성능(根瘤形成能) 및 질소고정능(窒素固定能)을 검토(檢討)하고자 동부, 녹두, 팥, 땅콩에 무접종(無接種)+N : 0kg/10a, 무접종(無接種)+N : 6kg/10a, 접종(接種)+N : 0kg/10a, 접종(接種)+N : 6kg/10a, 접종(接種)+N : 12kg/10a로 처리(處理)하여 시험(試驗)한 결과(結果) 초기근류형성시기(初期根瘤形成時期)는 팥이 가장 빠르고 동부, 녹두, 땅콩순(順)이었으며 질소(窒素)를 증시(增施)할 수록 지연(遲延)되는 경향(傾向)이었다. 근류수(根瘤數)와 근류중(根瘤重)은 생육(生育)이 진행(進行)될 수록 증가(增加)하였고, 질소(窒素)를 증시(增施)할 경우 땅콩을 제외한 동부, 녹두, 팥에서 감소(減少)하였다. 건물생산량(乾物生産量)과 질소흡수량(窒素吸收量)은 균접종구(菌接種區)가 무접종구(無接種區)에 비(比)해 높았고 균접종시(菌接種時)는 질소무시용구(窒素無施用區)에 비(比)해 질소(窒素) 6kg/10a 시용구(施用區)가 높았다. 접종균(接種菌)에 의해 고정(固定)된 질소량(窒素量)은 파종후(播種後) 60일(日) 질소무시용구(窒素無施用區)의 동부, 녹두, 팥, 땅콩이 각각(各各) 39.32, 42.39, 28.07, 78.56mg/주(株) 이었고 질소(窒素)6kg/10a 시용구(施用區)에서는 각각(各各) 25.46, 24.97, 16.80, 47.65mg/주(株)로서 질소무시용구(窒素無施用區)가 질소(窒素)6kg/10a시용구(施用區)에 비(比)해 많아 균접종효과는 질소시용구(窒素施用區)보다는 무시용구(無施用區)에서 뚜렷했다.

• 한국응용곤충학회지
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• 제58권1호
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• pp.49-54
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• 2019

클로버씨스트선충(Heterodera trifolii)은 우리나라 고랭지에서 배추에 피해를 주는 주요 씨스트선충으로 최근에 분포가 확인되었다. 본 연구는 온도와 접종밀도에 따른 클로버씨스트선충의 증식에 미치는 영향을 알아보기 위하여 수행하였다. $15{\sim}30^{\circ}C$ 온도조건에서 클로버씨스트선충이 증식되었으며 $20^{\circ}C$에서 암컷 성충 수, 알 수, 씨스트 수 모두 가장 많았다. 접종밀도에 따른 클로버씨스트선충의 증식은 접종밀도가 높아질수록 암컷 씨스트선충의 수가 증가하는 경향이었는데 토양 1 g당 16개의 알 처리 시 증식량, 알 수, 씨스트 수 모두 가장 많았다. 암컷 씨스트 수와 알 수는 접종밀도에 상관없이 배추가 케일에 비하여 많이 형성되어 배추가 케일보다 클로버씨스트선충의 증식에 유리한 기주였다.

• 한국작물학회지
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• 제24권2호
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• pp.75-81
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• 1979

우리나라 장여품종을 위함한 15개 대두품종들의 근류균에 대한 친화성과 접종효과를 밝히기 위하여 사경시험을 실시하였던 바 그 얻어진 결과를 요약하면 다음과 같다. 1. 정석은 근류균접종 15일후에도 근류가 형성되지 않았으나 Lindarin #62는 주당 3.0개의 근류가 형성 되었으며 개화기의 근류형성수도 품종간에 크게 차이가 있어서 정석은 주당 37.8대로 가장 적었고 Haman은 주당 122.2개로 가장 많이 형성되었는데 품종간에 유의차가 인정되었다. 2. 개화기까지의 근류형성수에 대한 시기별 근류형성비율은 품종간에 크게 차이가 있었는데 공시품종평균에 있어서는 접종 15일후에 2,1％, 접종30일후에는 60.8％, 그리고 접종 45일후에는 79.8％가 형성되었다. 3. 근류병접종효과는 현저하여 초장, 경태, 근장, 건물중 및 엽록소함량이 무접종구에 비하여 접종구가 크게 증가하여 전체처리 및 품종간, 그리고 접종처리간에 고도로 유의하였는데 다만 엽록소함량과 건물중의 증가추세와는 반드시 일치되지 않았다. 4. 공시품종평균에 있어서 접종구에 대한 무접종구의 몇가지 현질의 형성비율은 초장 94.0％, 건물중 81.6％, 그리고 엽록소의 함량은 66.8％였다.