• Title/Summary/Keyword: Injection process

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Development of Disposable Enzyme-linked Immunosensor Strip Platform (일회용 스트립형 효소면역센서용 플랫폼의 개발)

  • Choi, Ji-Hye;Yi, Seung-Jae;Chang, Seung-Cheol;Kim, Kyung-Chun
    • Journal of Sensor Science and Technology
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    • v.20 no.6
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    • pp.400-405
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    • 2011
  • This study introduced the development of a strip type disposable enzyme-linked immunosensor platform for the detection of IgG. Strips of the strip sensor were fabricated by using commercial nitrocellulose filter membranes and a housing holder for the strips was manufactured by using a standard injection molding process for a plastic material. An IgG-urease conjugate was prepared and used for the competitive immune-binding with sample IgG. From the enzymatic reaction between the conjugated urease and urea added, ammonia was generated and caused a localized alkaline pH change on the immobilized antibody band which was coated onto the sensor strips. This pH increase subsequently caused a color change of the antibody band in the presence of a pH indicator, phenol red. Used in conjunction with a competitive immunoassay format, the intensity of the color produced is directly linked with the concentration of target analyte, IgG, and specific measurement of IgG in a lateral flow immunoassay format was achieved over the range 100 ppb to 2000 ppb IgG.

CCD Image Sensor with Variable Reset Operation

  • Park, Sang-Sik;Uh, Hyung-Soo
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.3 no.2
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    • pp.83-88
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    • 2003
  • The reset operation of a CCD image sensor was improved using charge trapping of a MOS structure to realize a loe voltage driving. A DC bias generating circuit was added to the reset structure which sets reference voltage and holds the signal charge to be detected. The generated DC bias is added to the reset pulse to give an optimized voltage margin to the reset operation, and is controlled by adjustment of the threshold voltage of a MOS transistor in the circuit. By the pulse-type stress voltage applied to the gate, the electrons and holes were injected to the gate dielectrics, and the threshold voltage could be adjusted ranging from 0.2V to 5.5V, which is suitable for controlling the incomplete reset operation due to the process variation. The charges trapped in the silicon nitride lead to the positive and negative shift of the threshold voltage, and this phenomenon is explained by Poole-Frenkel conduction and Fowler-Nordheim conduction. A CCD image sensor with $492(H){\;}{\times}{\;}510(V)$ pixels adopting this structure showed complete reset operation with the driving voltage of 3.0V. The resolution chart taken with the image sensor shows no image flow to the illumination of 30 lux, even in the driving voltage of 3.0V.

Similarity between a stagnant point diffusion flame and an evolving jet diffusion flame (전개확산제트화염과 정체점 확산화염과의 유사성)

  • Park, Jeong;Shin, Hyun-Dong
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.21 no.4
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    • pp.494-502
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    • 1997
  • Experiments on corresponding jet flames with stagnant point diffusion flames have been carried out in initial injection periods. A compensated measurement of maximum flame temperature, which is based on the ion signal, has been employed to inspect flame responses to time-varying strain rates. The flame responses are obtained at two conditions for the slowly time-varying strain rate and the case of flame extinction, and analyzed to confirm similarity between a stagnant point diffusion flame and an evolving jet diffusion flame. Nonsteady effects are addressed via the comparison between several time scales. The time variation with low strain rates, in which illustrates the flame behavior of the upper branch far from extinction in the well-known S-curve, is confirmed to produce a quasi-steady flame response through the nonsteady experiments. The time variation with strain rates in the case of flame extinction indicates an unsteady effect of flame response. It is therefore found that the flame responses near jet tip depend on time histories of characterized strain rates in the developing process.

A Proposal for Diesel Spray Model Using a TAB Breakup Model and Discrete Vortex Method

  • Yeom, Jeong-Kuk;Lee, Myung-Jun;Chung, Sung-Sik;Ha, Jong-Yul;Jiro Senda;Hajime Fujimoto
    • Journal of Mechanical Science and Technology
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    • v.16 no.4
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    • pp.532-548
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    • 2002
  • A hybrid model consisting of a modified TAB (Taylor Analogy Breakup) model and DVM (Discrete Vortex Method) is proposed for numerical analysis of the evaporating spray phenomena in diesel engines. The simulation process of the hybrid model is divided into three steps. First, the droplet breakup of injected fuel is analyzed by using the modified TAB model. Second, spray evaporation is calculated based on the theory of Siebers'liquid length. The liquid length analysis of injected fuel is used to integrate the modified TAB model and DVM. Lastly, both ambient gas flow and inner vortex flow of injected fuel are analyzed by using DVM. An experiment with an evaporative free spray at the early stage of its injection was conducted under in-cylinder like conditions to examine an accuracy of the present hybrid model. The calculated results of the gas jet flow by DVM agree well with the experimental results. The calculated and experimental results all confirm that the ambient gas flow dominates the downstream diesel spray flow.

A Research on the Characteristics of Spray-Induced Mixing and Thermal Decomposition of Urea Solution in SCR System (SCR 시스템의 요소용액 미립화 및 분해반응 특성 예측에 관한 전산 해석 연구)

  • 김주연;민병수;하지수;류승협
    • Journal of Advanced Marine Engineering and Technology
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    • v.28 no.5
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    • pp.818-826
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    • 2004
  • The spray-induced mixing characteristics and thermal decomposition of aqueous urea solution into ammonia have been studied to design optimum sizes and geometries of the mixing chamber in SCR(Selective Catalytic Reduction) system. The cold flow tests about the urea-injection nozzle were performed to clarify the parameters of spray mixing characteristics such as mean diameter and velocity of drops and spray width determined from the interactions between incoming air and injected drops. Discrete particle model in Fluent code was adopted to simulate spray-induced mixing process and the experimental results on the spray characteristics were used as input data of numerical calculations. The simulation results on the spray-induced mixing were verified by comparing the spray width extracted from the digital images with the simulated Particle tracks of injected drops. The single kinetic model was adopted to predict thermal decomposition of urea solution into ammonia and solved simultaneously along with the verified spray model. The hot air generator was designed to match the flow rate and temperature of the exhaust gas of the real engines The measured ammonia productions in the hot air generator were compared with the numerical predictions and the comparison results showed good agreements. Finally, we concluded that the design capabilities for sizing optimum mixing chamber were established.

Effect of liquid viscosity on internal flow and spray characteristics of Y-jet atomizers (액체 점도에 따른 Y-jet 노즐 내부 유동 및 분무 특성의 변화)

  • Song, Si-Hong;Lee, Sang-Yong
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.20 no.12
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    • pp.4053-4061
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    • 1996
  • Internal flow characteristics within Y-jet atomizers and the local drop size distribution and cross-sectional averaged drop size at the outside were investigated with the liquid and air injection pressures, mixing port length of atomizers, and the liquid properties taken as parameters. To examine the effect of the liquid viscosity, glycerin-water mixtures were used in this study. The liquid viscosity plays only a minor role in determining the internal flow pattern and the spatial distribution shape of drops, but the drop sizes themselves generally increase with increasing of the liquid viscosity. An empirical correlation for the liquid discharge coefficient at the liquid port was deduced from the experimental results; the liquid discharge coefficient strongly depends on the liquid flow area at the mixing point which is proportional to the local volumetric quality(.betha.$_{Y}$), and the volumetric quality was included in the correlation. Regardless of the value of the liquid viscosity, the compressible flow through the gas port was well represented by the polytropic expansion process(k=1.2), and the mixing point pressure could be simply correlated to the aspect ratio( $l_{m}$/ $d_{m}$) of the mixing port and the air/liquid mass flow rate ratio( $W_{g}$/ $W_{f}$) as reported in the previous study.udy.udy.y.

Soot Deposition Process in a Diffusion Flame to the Wall under Microgravity (미소중력환경하에서의 확산화염내 매연입자의 벽면부착 관찰)

  • Choi, Jae-Hyuk;Fujita, Osamu;Yoon, Suck-Hun
    • Proceedings of the Korean Society of Marine Engineers Conference
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    • 2005.06a
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    • pp.87-92
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    • 2005
  • Experiments at the Japan Microgravity Center(JAMIC) have investigated the interaction between diffusion flames and solid surfaces placed near flames. The fuel for the flames was $C_2H_4$. The surrounding oxygen concentration was 35% with temperatures of $T_a$=300. Especially, the effect of wall temperature on soot deposition from a diffusion flame placed near the wall has been studied by utilizing microgravity environment, which can attain very stable flame along the wall. Cylindrical burner with fuel injection was adopted to obtain two dimensional soot distributions by laser extinction method. In the experiment two different wall temperatures, $T_w$=300,800K, were selected as test conditions. The results showed that the soot distribution between flame and burner wall was strongly affected by the wall temperature and soot deposition increases with decrease in wall temperature. The comparison among the values for two different wall temperatures suggested that the change in thermophoretic effect is the most dominant factor to give the change in soot deposition characteristics.

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Soft Lithographic Patterning Method for Flexible Graphene-based Chemical Sensors with Heaters

  • Kang, Min-a;Jung, Min Wook;Myung, Sung;Song, Wooseok;Lee, Sun Suk;Lim, Jongsun;Park, Chong-Yun;An, Ki-Seok
    • Proceedings of the Korean Vacuum Society Conference
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    • 2014.02a
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    • pp.176.2-176.2
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    • 2014
  • In this work, we demonstrated that the fabrication of flexible graphene-based chemical sensor with heaters by soft lithographic patterning method [1]. First, monolayer and multilayer graphene were prepared by thermal chemical vapor deposition transferred onto SiO2 / Si substrate in order to fabrication of patterned-sensor and -heater. Second, patterned-monolayer and multilayer graphene were detached through soft lithography process, which was transferred on top and bottom sides of PET film. Third, Au / Ti (Thickness : 100/30 nm) electrodes were deposited end of the patterned-graphene line by sputtering system. Finally, we measured sensor properties through injection of NO2 and CO2 gas on different temperature with voltage change of graphene heater.

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A Study on the Microstructures and Properties of $Al-SiC)_p$ Metal Matrix Composites Fabricated by Spray Forming Process (분무성형법에 의해 제조된 $Al-SiC)_p$ 금속기 복합재료의 미세조직과 성질에 관한 연구)

  • 김춘근
    • Journal of Powder Materials
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    • v.1 no.1
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    • pp.42-51
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    • 1994
  • 6061Al-SiCP metal matrix composite materials(MMCs) were fabricated by injecting SiCP particles directly into the atomized spray. The main attraction of this technique is the rapid fabrication of semi-finished, composite products in a combined atomization, particulate injection(10 $\mu\textrm{m}$, 40 $\mu\textrm{m}$, SiCP) and deposition operation. Conclusions obtained are as follows; The microstructure of the unreinforced spray formed 6061Al alloy consisted of relatively fine(50 $\mu\textrm{m}$) equiaxed grains. By comparision, the microstructure of the I/M materials was segregated and consisted of relatively coarse(150 $\mu\textrm{m}$) grains. The probability of clustering of SiCP particles in co-sprayed metal matrix composites increased it ceramic particle size(SiCP) was reduced and the volume fraction was held constant. Analysis of overspray powders collected from the spray atomization and deposition experiments indicated that morphology of powders were nearly spherical and degree of powders sphercity was deviated due to composite with SiCp particles. Interfacial bonding between matrix and ceramics was improved by heat treatment and addition of alloying elements(Mg). Maximum hardness values [Hv: 165 kg/mm2 for Al-10 $\mu\textrm{m}$ SiCp Hv--159 kg/mm2 for Al-40 $\mu\textrm{m}$SiCp] were obtained through the solution heat treatment at $530^{\circ}C$ for 2 hrs and aging at $178^{\circ}C$, and there by the resistance were improved.

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Study of toluene decomposition using nonthermal plasma and catalyst (저온플라즈마와 촉매를 이용한 톨루엔 분해 연구)

  • Lim, Yun Hui;Lee, Ju-Yeol;Shin, Jae-Ran;Choi, Jin-Sik;Park, Byung Hyun
    • Journal of the Korean Applied Science and Technology
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    • v.31 no.4
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    • pp.541-548
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    • 2014
  • This study was performed to obtain high conversion efficiency of $C_7H_8$ using non-thermal plasma and metal-supported catalyst. Adsorption-desorption characteristics of toluene was performed using 4A type (Zeolite) filled in a concentration reactor. Through this test, it was found that the concentration reactor has 0.020 g/g of adsorption capacity (at ambient temperature and pressure) and 3,600 ppm of desorption property at $150^{\circ}C$ (with in 20 min). In case of developed catalyst, toluene decomposition rate of Pd-AO (Pd coated catalyst) was better than Pd/Cu-AO and Pd/Ag-AO (Pd/Ag composite metal catalyst). Developed non-thermal plasma system was obtained flame amplification effect using injection process of desorbed tolune, and 98% of removal efficiency.