Journal of Sensor Science and Technology (센서학회지)

The Korean Sensors Society (한국센서학회)
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Development of Disposable Enzyme-linked Immunosensor Strip Platform

일회용 스트립형 효소면역센서용 플랫폼의 개발

  • Choi, Ji-Hye (Institute of BioPhysio Sensor Technology, Pusan National University) ;
  • Yi, Seung-Jae (School of Mechanical Engineering, Pusan National University) ;
  • Chang, Seung-Cheol (Institute of BioPhysio Sensor Technology, Pusan National University) ;
  • Kim, Kyung-Chun (School of Mechanical Engineering, Pusan National University)
  • 최지혜 (부산대학교 바이오피지오센서연구소) ;
  • 이승재 (부산대학교 기계공학부) ;
  • 장승철 (부산대학교 바이오피지오센서연구소) ;
  • 김경천 (부산대학교 기계공학부)
  • Received : 2011.10.17
  • Accepted : 2011.10.27
  • Published : 2011.11.30
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Abstract

This study introduced the development of a strip type disposable enzyme-linked immunosensor platform for the detection of IgG. Strips of the strip sensor were fabricated by using commercial nitrocellulose filter membranes and a housing holder for the strips was manufactured by using a standard injection molding process for a plastic material. An IgG-urease conjugate was prepared and used for the competitive immune-binding with sample IgG. From the enzymatic reaction between the conjugated urease and urea added, ammonia was generated and caused a localized alkaline pH change on the immobilized antibody band which was coated onto the sensor strips. This pH increase subsequently caused a color change of the antibody band in the presence of a pH indicator, phenol red. Used in conjunction with a competitive immunoassay format, the intensity of the color produced is directly linked with the concentration of target analyte, IgG, and specific measurement of IgG in a lateral flow immunoassay format was achieved over the range 100 ppb to 2000 ppb IgG.

Keywords

References

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