20 bacterial strains capable of growing on phenol minimal medium were isolated from soil and wastewater by the enrichment culture technique, and among them, one isolate which was the best in the cell growth was selected and identified as Bacillus sp. SH3 by its characteristics. Strain SH3 could grow with phenol as the sole carbon source up to 15 mM, but did not grow in minimal medium containing above 20 mM of phenol. The optimal conditions of temperature and initial pH for growth and phenol degradation were 30$^{\circ}$C and 7.5, respectively. This strain could grow on various aromatic compounds such as catechol, protocatechuic acid, gentisic acid, o-, m-, p-cresol, benzoic acid, p-hydroxybenzoic acid, anthranilic acid, phenyl acetate and pentachlorophenol, and the growth-limiting log P value of strain SH3 on organic solvents was 3.1. In batch culture, strain SH3 degraded 97% of 10 mM phenol in 48 hours. In continuous culture under the conditions of 20 mM of influent phenol concentration and 0.050 hr$^{-1}$ of dilution rate, the treatment rate of phenol was 94%.
Lee Jeong, Mi-Jeong;Gillis, James M.;Hwang, Jiann-Yang
Bulletin of the Korean Chemical Society
/
v.24
no.12
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pp.1763-1766
/
2003
Algal growth studies of Chlorella strains were conducted in a batch mode with bench type experiments. Carbon dioxide fixation rates of the following green microalgae were determined: Chlorella sp. H84, Chlorella sp. A2, Chlorella sorokiniana UTEX 1230, Chlorella vulgaris, and Chlorella pyrenoidosa. C. vulgaris, among other strains of microalgae, showed the highest growth rate (1.17 optical density/5 days). Cultivating conditions for C. vulgaris that produced the highest growth rate were at concentrations of 243 ${\mu}g\;CO_2$/mL, 10 mM ammonia, and 1 mM phosphate, with an initial pH range of 7-8.
An 8-wk experiment was conducted to evaluate the effect of replacing fish meal (FM) with soy protein isolate (SPI) on the growth, digestive enzyme activity and serum biochemical parameters of juvenile Amur sturgeon (Acipenser schrenckii). SPI was used to replace 0, 25, 50, 62.5, 75, 87.5, 100% of dietary FM and 100% replacement supplemented crystalline amino acid. Healthy sturgeon with an average initial weight of $26.38{\pm}0.24$ g were randomly assigned to 24 aquaria (8 treatments with triplicates each) at an initial stocking density of 11 fish per aquarium and cultured for 8 wks. The results showed that 75.00% or more substitution resulted in a poor weight gain rate, feed conversion ratio and survival rate compared to that of fish fed the control diet (p<0.05), whereas no significant differences were observed between diets of 25.00% to 62.50% substitution. Protease, lipase and amylase activity in foregut, mid-gut and hindgut were significantly (p<0.05) decreased by diets where SPI replacement levels were 62.50% or more. Levels of serum total protein (TP) and globulin decreased significantly from 21.03, 10.34 to 14.05, 5.63 g/L with the increasing dietary SPI (p<0.05), but alkaline phosphatase activity significantly increased (p<0.05). In addition, supplemental crystalline amino acid in the FM absence diet did not improve growth performance, intestine digestive enzyme activities and serum biochemical parameters. In conclusion, the results from this study showed adverse effects of inclusion of SPI in diets on growth performance, feed utilization and serum biochemical parameters in juvenile Amur sturgeon. Based on WGR and replacement ratio presented in this report, a 57.64% replacement level was recommended.
Photosynthetic parameters of Scendesmus quadricauda, such as the maximum photosynthetic rate ($P_{max}$), photosynthetic efficiency (α) and the initial saturation intensity of irradiance for photosynthesis ($I_K$) were obtained using photosynthesis-irradiance (P-I) curve in a phosphorus-limited chemostat. S. quadricauda exhibitied no photoinhibition until at 200 μmol·$m^{-2}$ . $P_{max}$ (r=0.963, P=0.002) and $I_K$(r=0.904, P=0.013) showed linear relationships with growth rate. Chlorophyll-α concentration and cell dry weight decreased at higher growth rates, ut chlorophyll-α content per cell dry weight increased. The increase in photosynthetic rates at higher growth rates was due to the increase of $P_{max}$ and $I_K$ which was caused mainly by the increase in the absolute amount of chlorophyll-α rather than the increased photosynthetic efficiency of individual chlorphyll-α. The α did not show a significant relationship with growth rate (r=0.714, P=0.111). The cell quota of carbon (r=0.554, P=0.254) was not correlated with growth rate, but cell quota of nitrogen (r=0.818, P=0.047) and phosphorus (r=0.855, P=0.030) exhibited linear correlations with growth rate.
Kim, Tae-Keun;Kim, Hyoun-Chol;Song, Jin-Young;Lee, Hee-Seon;Ko, Seok-Hyung;Lee, You-mi;Song, Chang-Khil
Korean Journal of Plant Resources
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v.28
no.2
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pp.243-252
/
2015
This study was performed to establish a production system for in situ and ex situ conservation of Elsholtzia minima Nakai, an endemic plant grown in Jeju Island. Moreover, this study aimed to identify root-growth characteristics according to the use of pre-treatment agents and seedling growth effect according to fertilizer application. The mean temperature was similar in greenhouse and vinyl-moist chamber, but air humidity was higher in vinyl-moist chamber than in greenhouse. After stem planting of Elsholtzia minima Nakai, initial root growth was observed after 10 days in greenhouse and after 7 days in vinyl-moist chamber. Root growth rate was more rapid in vinyl-moist chamber. Moreover, survival rate, root growth rate and root number was slightly higher in vinyl-moist chamber than in greenhouse, indicating that vinyl-moist chamber is more effective in plant growth. When pre-treatment agents were used to remove root growth-inhibiting substances, a higher root growth rate of more than 95% was found in pre-treatment groups, excluding the group treated with AgNO3 at 77.5%. Thus, Elsholtzia minima Nakai is thought to have less root growth inhibitors. In the analysis of nitrogen application rate and Osmocote application by seedling container, a difference was found in survival rate and growth according to application rate and container conditions. When Osmocote, a slow release fertilizer, was applied to the soil surface around each culture container, survival rate and the growth of aerial and root parts were most favorable. Thus, Osmocote fertilizer is thought to be desirable for seedling propagation of Elsholtzia minima Nakai.
Journal of the Korea Society of Computer and Information
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v.24
no.6
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pp.189-197
/
2019
The purpose of this study was to investigate about the effects of parental rearing behavior on self-esteem and gender role stereotype during adolescence and to reveal the mediating effect of self-esteem on adolescents' gender role strerotype. Also investigated the relationship among these variables and condition variables. For this purpose, used the Second Grade longitudianl Panel data of Middle School from the Korea Youth Panel Survay(KYPS). And the latent growth model was analyzed 3,449 men and women adolescents' cases of the first, the fourth and the sixth wave of the Korea Youth Panel Survey(KYPS) administered by Korea Institute for Youth development. And the structural equation model was used to investigate whether self-esteem mediates parental rearing behavior and male and female gender role Strerotype. The results of this study, the direct effect between variable factors showed that the more positive the parenting behavior of the second grader of middle school is, the more positive the self-esteem of male adolescents and the initial value of stereotypes of male gender role. It also affects the self-esteem of female adolescents and stereotypes of female gender roles but not statistically significant. The male gender role stereotypes were decreased in influence by the rate of change of parental rearing behaviors, and the initial value and the rate of change of self-esteem were statistically influenced to the male gender role stereotypes and the higher the self-esteem, the higher the self-esteem. However, there was no significant effect on stereotypes of female gender roles. As a result of analyzing the mediating effect of self-esteem, partially mediated between the initial value of parental rearing behavior and initial stereotype of male gender role, but there was no longitudinal mediation effect. There was no mediating effect of self-esteem between parental rearing behaviors and stereotypes of female gender roles and there was no longitudinal mediation effect. The effect of condition variable gender was found that female adolescents are more affected by paretal rearing behavior than male adolescents and also affected by the growth process. The monthly income of households influenced the initial value of parental rearing behaviors and showed a difference in parental rearing behavior according to household income. And the higher the mother 's educational level, the more the self-esteem of male adolescents was affected.
This study was carried out to investigate the growth and branching pattern of stolons at Korean lawn(Zoysia japonica Steud.) in the field condition. The results were summarized as follows ; 1.About 80% of observed lawns had one primary stolon. Among the lawns with several primary stolons, 30% of them had two primary stolons, 63% had three, and 7% had four. 2.The angles between the primary stolon and the shoot were increased from 0˚ to 52˚ according to the node order from the terminal shoot apex, and reached maximum angle at the 7th node. 3.The internode length was the longest in the middle position of stolon, and its growing rate which depends on months was increased from May to September. 4.The branching angles between primary and secondary stolon were from 44˚ to 53˚ in each node. The average left branching angle was about 48.20, right branching angle 47.8˚, and the total average branching angle was 48.00. 5.The rate of initial brabch was the highest at the 10th node in May, the 7th node in July and the 5th node in September. But, the initial branching rate at the 7th node in July was higher than any other that at the 10th node in May and the 5th node in September. 6.The distribution rate of secondary stolon in each node of primary stolon was the highest at the 7th node. 7.when the terminal shoot apex of primary stolon was damaged mechanically, the branching rate at the first node after the damaged region was highly increased to 62%. The results of this study may be suggested that the secondary stolon begins to branch with the angle of 480 from the 7th node of the normal primary stolon, and those may be used as a basic data for the branching simulation in lawn.
Journal of the Korean Society of Environmental Restoration Technology
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v.19
no.6
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pp.77-86
/
2016
Due to aging of the rural and mountain populations the labor force is reduced. However work intensity continues to increase, thus, there is a need to improve the current effectiveness of forest operations. This study compared and analyzed the Operation productivity and efficiency of planting containerized seedlings using a battery-powered planting auger and a mattock. Growth was also investigated by looking into the initial growth increments in the planted seedlings. Tasks were investigated by analyzing the process and operation time needed to plant 1 containerized seedling using a planting auger and a mattock. The time spent on the various elements of the planting process was measured with a stopwatch but observations were done continuously. Result of the study shows that with the use of a lightweight planting auger the average time spent to plant a containerized seedling is 18.61 seconds while with the use of a mattock it took an average of 26.96 seconds which clearly demonstrates that the planting auger is more efficient in terms of working hours. Working intensity was also analyzed with the use of a portable heart rate monitor (Polar V800). The average increase in heart rate and work intensity index were analyzed for one planting cycle. It was observed that when using the lightweight planting auger, there was a 46.51% increase in the average heart rate while a 74.67% increase in heart rate when the mattock was used which shows that there is a significant increase in heart rate when mattock is used. In addition, work intensity index was observed to be 29.95% and 47.83% when the planting auger and mattock were used respectively. With the continuous use, work intensity index is significantly higher with the use of the mattock as compared to that of the lightweight planting auger. There were no significant differences on the growth increment of seedlings planted using the different tools until a year after planting, however differences in growth increment were observed after a year. A difference of 15.1 cm in height and 3.41 mm in diameter was observed which shows that the use of lightweight planting auger is excellent for planting containerized seedlings.
Variation of sintered density of BaTiO3 powder calcined at 120$0^{\circ}C$ and 135$0^{\circ}C$ was investigated with respect to the grain growth behavior. It was found that BaTiO3 powder, which was calcined at 120$0^{\circ}C$, showed abnormal grain growth behavior during sintering process. At initial stage of sintering process, the densification rate of specimen was accelerated with rapid grain growth caused by the abnormal grain growth. But with the increase of sintering time, abnormally grown grain met each other and the density of specimen decreased drastically due to coalescence of pores located in triple junction. On the contrary, BaTiO3 powder calcined at 135$0^{\circ}C$ showed normal grain growth behavior and gradually densified with the increase of sintering time.
Fresh ginseng of same grade was stored under the 4$\pm$1$^{\circ}C$ and 87~92% RH for 10 weeks. During the storage, an aliquot amount of the ginseng was drawn, freeze dried and chemical constituents and physicochemical parameters were measured. After 10 weeks of storage drying rate and shrinkage of ginseng were 1520% and 9.04%, respectively, mold growth was seen at week 5 and observed for 51.2% of the ginseng week 10. Amylase activity level was elevated at the early stage of storage and decreased to 5% of initial value at week 5. At week 5, the elevated amylase activity was inconcomitant with the appearance of the mold growth. Crude protein contents were increased and decreased, respectively 5 week post storage. No significant changes in crude fat, crude fiber, ash, total sugar, n-butanol extract and ginsenoside were observed. The content of water-extractable substance showed maximum at week 7 to 8. The value of pH was slightly elevated and reducing sugar was increased during the storage. Key words Ginseng storage, physicochemical properties, drying rate, shrinkage, amylase activity.
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