• Journal of Microbiology and Biotechnology
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• 제18권9호
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• pp.1584-1589
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• 2008

Although resistance of Helicobacter pylori to clarithromycin is a major cause of failure of eradication therapies, little information is available regarding gene mutations of clarithromycin-resistant primary and secondary H. pylori isolates in Korea. In the present study, we examined gene mutations of H. pylori 238 rRNA responsible for resistance to clarithromycin. DNA sequences of the 238 rRNA gene in 21 primary clarithromycin-resistant and 64 secondary clarithromycin-resistant strains were determined by PCR amplification and nucleotide sequence analyses. Two mutations of the 238 rRNA gene, A2143G and T2182C, were observed in primary clarithromycin-resistant isolates. In secondary isolates, dual mutation of A2143G+T2182C was frequently observed. In addition, A2143G+T2182C+ T2190C, A2143G+T2182C+C2195T, and A2143G+T2182C+A2223G were observed in secondary isolates. Furthermore, macrolide binding was tested on purified ribosomes isolated from T2182C or A2143C mutant strains with $[^{14}C]$erythromycin. Erythromycin binding increased in a dose-dependent manner for the susceptible strain but not for the mutant strains. These results indicate that secondary isolates show a greater variety of 238 rRNA gene mutation types than primary isolates, and triple mutations of secondary isolates are associated with A2143G+T2182C in H. pylori isolated from Korean patients.

• 한국융합학회논문지
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• 제8권8호
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• pp.225-230
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• 2017

시조는 우리의 삶에 역동적이고 정격화 된 치유의 활력을 제공한다. 본 연구는 시조가 명사로 끝나는 부분에서 발화하는 문학치료의 기능을 살펴보고자 하였다. 연구의 결과 시조의 종장에 사용된 명사는 서술어의 기능을 발화시키는 것이었다. 이 시조는 한 음보 단축된 11음보로 응집되면서도 12음보의 기능으로 발화되는 것이다. 이러한 시조의 기능 발화는 문학치료의 부호화에 대한 치료적 서술이다. 그래서 시조나 언어에서 서술하지 않고 명사만 언급해도 치유적인 부호화를 활성화시킬 수 있다. 그 이유는 시조의 명사가 인체에 끼어들어 주어가 되고 인체의 뉴런이 서술어가 되어 문장을 만들어내기 때문이다. 이때 뉴런의 핵이 명사로 들어온 정보를 분석하여 새로운 문장을 만들어낸 것을 인체가 인식하는 것으로 판단된다. 이러한 인식이 또한 인체에서 발화하는 치료의 기능인 부호화의 과정이다.

• Journal of Plant Biotechnology
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• 제45권2호
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• pp.102-109
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• 2018

엉겅퀴는 일반적으로 이용되는 대표적인 다년생의 약용식물이다. 최근 국제적 추세에 따라 자국의 유전자원의 발굴, 보존 등이 강화됨에 따라 인접국가와 국내 자생 엉겅퀴 계통을 판별 할 수 있는 기준 설정에 관한 연구의 필요성이 대두되고 있지만, 분자생물학적 판별 기술의 개발은 아직 미흡한 실정이다. 본 연구에서는 국내 토종과 해외 유래 엉겅퀴종의 기원을 판별하기 위해 핵의 리보솜에 존재하는 ITS 유전자단편에서 SNP를 이용한 판별 프라이머를 확보하였으며, 이를 보완하여 보다 신속하게 판별하기 위하여 ARMS-PCR 및 HRM 기술을 이용한 판별 마커와 그 조건을 확립하였다. 또한, 국내 종 특이적 프라이머들을 이용한 정량적 PCR 분석방법을 이용해 두 가지 종의 genomic DNA의 혼합 여부를 판별하였다. 그러므로, 본 연구에서 개발된 SNP 마커는 다양한 지역 또는 국가에서 서식하는 엉겅퀴 종들의 신속한 확인을 위해 매우 유용하게 이용될 것으로 생각된다.

• Asian-Australasian Journal of Animal Sciences
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• 제19권5호
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• pp.622-626
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• 2006

Molecular characterization of Hallikar, the native cattle breed of Karnataka, was undertaken using 19 cattle specific, highly polymorphic microsatellite markers recommended by FAO. The genomic DNA was subjected to PCR amplification and alleles were resolved through six per cent denaturing PAGE with a 10 bp DNA ladder followed by silver staining. Genotyping of animals was done based on allele size. The number of alleles ranged from three to nine with allele sizes ranging from 102 bp to 294 bp. These alleles were distributed in the frequency range between 0.0306 and 0.8673 in the population. The mean observed number of alleles was $6.368{\pm}1.4225$. The mean observed and expected heterozygosities were $0.7515{\pm}0.1734$ and $0.7850{\pm}0.1381$, respectively. The high heterozygosity observed implies presence of higher genetic variability within Hallikar breed. The PIC (Polymorphism Information Content) values ranged from 0.2322 (ETH152) to 0.8654 (ETH225). The percentage of polymorphic loci obtained was 100 as all the 19 microsatellite markers were found to be polymorphic. Except for ETH152, all the other loci had high PIC values, indicating that these markers are highly informative for characterization of Hallikar breed. The population was tested for Hardy-Weinberg equilibrium at 19 microsatellite loci, and at 74 per cent of the loci the population was found to be in disequilibrium.

• Asian-Australasian Journal of Animal Sciences
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• 제20권10호
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• pp.1473-1477
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• 2007

Four-and-a-half LIM-only protein 3 (FHL3) is a member of the LIM protein superfamily and can participate in mediating protein-protein interaction by binding one another through their LIM domains. In this study, the 5'- and 3'- cDNA ends were characterized by RACE (Rapid Amplification of the cDNA Ends) methodology in combination with in silico cloning based on the partial cDNA sequence obtained. Bioinformatics analysis showed FHL3 protein contained four LIM domains and four LIM zinc-binding domains. In silico mapping assigned this gene to the gene cluster MTF1-INPP5B-SF3A3-FHL3-CGI-94 on pig chromosome 6 where several QTL affecting intramuscular fat and eye muscle area had previously been identified. Transcription of the FHL3 gene was detected in spleen, liver, kidney, small intestine, skeletal muscle, fat and stomach, with the greatest expression in skeletal muscle. The A/G polymorphism in exon II was significantly associated with birth weight, average daily gain before weaning, drip loss rate, water holding capacity and intramuscular fat in a Landrace-derived pig population. Together, the present study provided the useful information for further studies to determine the roles of FHL3 gene in the regulation of skeletal muscle cell growth and differentiation in pigs.

• 대한전자공학회논문지SD
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• 제47권12호
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• pp.1-7
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• 2010

본 논문은 멀티레벨을 갖는 PoRAM 셀의 데이터를 센싱하는 기법에 관하여 제안하였다. PoRAM은 유기물질을 사용한 단위 셀의 상,하단 전극에 전압을 가했을 때 나타나는 저항 상태의 변화로 셀 데이터를 구분하는 메모리 소자이다. 특히 한 셀당 최대 4 레벨의 안정된 저항 값을 가지므로 멀티레벨 셀로 활용이 가능하다. 따라서 멀티레벨의 센싱을 위해 어드레스 디코딩 방법, 센스 앰플리파이어, 이를 위한 제어 신호 등을 새롭게 제안하였다. 센스 앰플리파이어는 셀에 흐르는 전류를 입력 값으로 받아 설정된 기준 전류($I_{REF}$)와 비교하는 전류 비교기를 기본으로 구성되며 전류를 증폭하기 위해 낮은 입력 임피던스를 갖도록 설계되었다. 제안된 기법에 의해 설계된 회로는 $0.13{\mu}m$ CMOS 공정 라이브러리를 사용하여 설계되었고, 이를 사용함으로써 단위 셀에 흐르는 서로 다른 4 가지 전류 값이 각각 데이터 "00", "01", "10", "11"으로 정확히 센싱 되는 것을 검증하였다.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2009년도 정보 및 제어 심포지움 논문집
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• pp.357-359
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• 2009

In this paper, the 2.4GHz doppler radar system consisting of the doppler radar module and a baseband module were designed to detect heartbeat and respiration signal without direct skin contact. A bio-radar system emits continuous RF signal of 2.4GHz toward human chest, and then detects the reflected signal so as to investigate cardiopulmonary activities. The heartbeat and respiration signals acquired from quadrature signal of the doppler radar system are applied to the pre-processing circuit, amplification circuit, and the offset circuit of the baseband module. ECG(electrocardiogram) and reference respiration signals are measured simultaneously to evaluate the doppler radar system. As a result, the respiration signal of doppler radar signal is detected to 1m without complex digital signal processing. The sensitivity and calculated from I/Q respiration signal were $98.29{\pm}1.79%$, $97.11{\pm}2.75%$, respectively, and positive predictivity were $98.11{\pm}1.45%$, $92.21{\pm}10.92%$, respectively. The sensitivity and positive predictivity calculated from phase and magnitude of the doppler radar were $95.17{\pm}5.33%$, $94.99{\pm}5.43%$, respectively. In this paper, we confirmed that noncontact real-time heartbeat and respiration detection using the doppler radar system has the possibility and limitation.

• Parasites, Hosts and Diseases
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• 제55권3호
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• pp.351-355
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• 2017

Hymenolepis nana and Hymenolepis diminuta are globally widespread zoonotic cestodes. Rodents are the main reservoir host of these cestodes. Brown rats (Rattus norvegicus) are the best known and most common rats, and usually live wherever humans live, especially in less than desirable hygiene conditions. Due to the little information of the 2 hymenolepidid species in brown rats in China, the aim of this study was to understand the prevalence and genetic characterization of H. nana and H. diminuta in brown rats in Heilongjiang Province, China. Total 114 fecal samples were collected from brown rats in Heilongjiang Province. All the samples were subjected to morphological examinations by microscopy and genetic analysis by PCR amplification of the mitochondrial cytochrome c oxidase subunit 1 (COX1) gene and the internal transcribed spacer 2 (ITS2) region of the nuclear ribosomal RNA gene. In total, 6.1% (7/114) and 14.9% (17/114) of samples were positive for H. nana and H. diminuta, respectively. Among them, 7 and 3 H. nana isolates were successfully amplified and sequenced at the COX1 and ITS2 loci, respectively. No nucleotide variations were found among H. nana isolates at either of the 2 loci. Seventeen H. diminuta isolates produced 2 different COX1 sequences while 7 ITS2 sequences obtained were identical to each other. The present results of H. nana and H. diminuta infections in brown rats implied the risk of zoonotic transmission of hymenolepiasis in China. These molecular data will be helpful to deeply study intra-specific variations within Hymenolepis cestodes in the future.

• 비파괴검사학회지
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• 제35권2호
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• pp.128-133
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• 2015

원격진료를 요하는 응급현장에서 사용을 위하여 1차원 배열 탐촉자를 손으로 조작하는 동안 변하는 방사빔의 위치를 추적하여 정적인 3차원 초음파영상을 구성하였다. 탐촉자에 고정된 모션센서의 병진운동 및 회전운동에 대한 정보를 기준직교좌표계로 변환하는 이론적 모델을 제시하였으며, 신호 증폭과 시리얼 통신을 위한 인터페이스 용도의 모듈을 상용의 센서를 사용하여 제작하였다. 실리콘 퍼티로 제작된 시험용 팬텀에 대한 B-모드의 동영상과 센서의 신호를 동기화하여 기록하였다. 동영상으로부터 주기적으로 추출한 B-모드 영상과 각 영상내의 픽셀 각각의 그레이 레벨을 이용하여 3차원 복셀의 그레이 레벨을 재구성하였다. 이 복셀의 데이터를 이용하여 3차원 영상과 임의단면의 B-모드 방식의 2차원 영상을 구성하였으며, 이 영상들은 시험용 팬텀의 형상과 잘 일치하였다.

• Parasites, Hosts and Diseases
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• 제53권1호
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• pp.113-117
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• 2015

Cryptosporidium spp., ubiquitous enteric parasitic protozoa of vertebrates, recently emerged as an important cause of economic loss and zoonosis. The present study aimed to determine the distribution and species of Cryptosporidium in post-weaned and adult pigs in Shaanxi province, northwestern China. A total of 1,337 fresh fecal samples of post-weaned and adult pigs were collected by sterile disposable gloves from 8 areas of Shaanxi province. The samples were examined by Sheather's sugar flotation technique and microscopy at${\times}400$ magnification for Cryptosporidium infection, and the species in positive samples was further identified by PCR amplification of the small subunit (SSU) rRNA gene. A total of 44 fecal samples were successfully amplified by the nested PCR of the partial SSU rRNA, with overall prevalence of 3.3%. The average prevalence of Cryptosporidium infection in each pig farms ranged from 0 to 14.4%. Species identification by sequencing of SSU rRNA gene revealed that 42 (3.1%) samples were Cryptosporidium suis and 2 (0.15%) were Cryptosporidium scrofarum. C. suis had the highest prevalence (7.5%) in growers and the lowest in breeding pigs (0.97%). C. suis was the predominant species in pre-weaned and adult pigs, while C. scrofarum infected pigs older than 3 months only. A season-related difference of C. suis was observed in this study, with the highest prevalence in autumn (5.5%) and the lowest (1.7%) in winter. The present study provided basic information for control of Cryptosporidium infection in pigs and assessment of zoonotic transmission of pigs in Shaanxi province, China.