Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Full-length cDNA, Expression Pattern and Association Analysis of the Porcine FHL3 Gene

  • Zuo, Bo (Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture & Key Lab of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University) ;
  • Xiong, YuanZhu (Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture & Key Lab of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University) ;
  • Yang, Hua (Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture & Key Lab of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University) ;
  • Wang, Jun (Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture & Key Lab of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University)
  • Received : 2006.11.08
  • Accepted : 2007.04.07
  • Published : 2007.10.01
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Abstract

Four-and-a-half LIM-only protein 3 (FHL3) is a member of the LIM protein superfamily and can participate in mediating protein-protein interaction by binding one another through their LIM domains. In this study, the 5'- and 3'- cDNA ends were characterized by RACE (Rapid Amplification of the cDNA Ends) methodology in combination with in silico cloning based on the partial cDNA sequence obtained. Bioinformatics analysis showed FHL3 protein contained four LIM domains and four LIM zinc-binding domains. In silico mapping assigned this gene to the gene cluster MTF1-INPP5B-SF3A3-FHL3-CGI-94 on pig chromosome 6 where several QTL affecting intramuscular fat and eye muscle area had previously been identified. Transcription of the FHL3 gene was detected in spleen, liver, kidney, small intestine, skeletal muscle, fat and stomach, with the greatest expression in skeletal muscle. The A/G polymorphism in exon II was significantly associated with birth weight, average daily gain before weaning, drip loss rate, water holding capacity and intramuscular fat in a Landrace-derived pig population. Together, the present study provided the useful information for further studies to determine the roles of FHL3 gene in the regulation of skeletal muscle cell growth and differentiation in pigs.

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References

  1. Coghill, I. D., S. Brown, D. L. Cottle, M. J. McGrath, P. A. Robinson, H. H. Nandurkar, J. M. Dyson and C. A. Mitchell. 2003. FHL3 is an actin-binding protein that regulates alphaactinin- mediated actin bundling: FHL3 localizes to actin stress fibers and enhances cell spreading and stress fiber disassembly. J. Biol. Chem. 278(26):24139-24152. https://doi.org/10.1074/jbc.M213259200
  2. Cottle, D. L., M. J. McGrath, B. S. Cowling, I. D. Coghill, S. Brown and C. A. Mitchell. 2007. FHL3 binds MyoD and negatively regulates myotube formation. J. Cell Sci. 120: 1423-1435. https://doi.org/10.1242/jcs.004739
  3. Dai, L. H., Y. Z. Xiong, C. Y. Deng, S. W. Jiang, B. Zuo, R. Zheng, F. E. Li and M. G. Lei. 2006. Association of the A-G polymorphism in porcine adiponectin gene with fat deposition and carcass traits. Asian-Aust. J. Anim. Sci. 19(6):779-783. https://doi.org/10.5713/ajas.2006.779
  4. Fimia, G. M., D. De Cesare and P. Sassone-Corsi. 2000. A family of LIM-only transcriptional coactivators: tissue-specific expression and selective activation of CREB and CREM. Mol. Cell. Biol. 20:8613-8622. https://doi.org/10.1128/MCB.20.22.8613-8622.2000
  5. Goureau, A., M. Yerle, A. Schmitz, J. Riquet, D. Milan, P. Pinton, G. Frelat and J. Gellin. 1996. Human and porcine correspondence of chromosome segments using bidirectional chromosome painting. Genomics 36(2):252-262. https://doi.org/10.1006/geno.1996.0460
  6. Lee, S. M., S. K. W. Tsui, K. K. Chan, M. Kotaka, H. Y. Li, S. S. C. Chim, M. M. Y. Waye, K. P. Fung and C. Y. Lee. 1998. Chromosomal mapping of a skeletal muscle specific LIM-only protein FHL3 to the distal end of the short arm of human chromosome 1. Somatic Cell Mol. Genet. 24:197-202. https://doi.org/10.1023/B:SCAM.0000007122.03392.4b
  7. Li, H. Y., E. K. Ng, S. M. Lee, M. Kotaka, S. K. Tsui, C. Y. Lee, K. P. Fung and M. M. Waye. 2001. Protein-protein interaction of FHL3 with FHL2 and visualization of their interaction by green fluorescent proteins (GFP) two-fusion fluorescence resonance energy transfer (FRET). J. Cell Biochem. 80(3):293-303. https://doi.org/10.1002/1097-4644(20010301)80:3<293::AID-JCB10>3.0.CO;2-U
  8. Liu, B. H. 1998. Statistical Genomics: Linkage, Mapping, and QTL Analysis. CRC Press, LLC, pp. 404-409.
  9. Mils, V., S. M. Lee, W. Joly, E. W. Hang, V. Baldin, M. M. Waye, B. Ducommun and S. K. Tsui. 2003. LIM-only protein FHL3 interacts with CDC25B2 phosphatase. Exper. Cell Res. 285(1): 99-106. https://doi.org/10.1016/S0014-4827(03)00018-1
  10. Morgan, M. J. and A. J. Madgwick. 1999. The LIM proteins FHL1 and FHL3 are expressed differently in skeletal muscle. Biochem. Biophy. Res. Comm. 255:245-250. https://doi.org/10.1006/bbrc.1999.0179
  11. Ovilo, C., M. Perez-Enciso, C. Barragan, A. Clop, C. Rodriguez, M. A. Oliver, M. A. Toro and J. L. Noguera. 2000. A QTL for intramuscular fat and backfat thickness is located on porcine chromosome 6. Mamm. Genome 11:344-340. https://doi.org/10.1007/s003350010065
  12. Ovilo, C., A. Clop, J. L. Noguera, M. A. Oliver, C. Barragan, C. Rodriguez, L. Silio, M. A. Toro, A. Coll, J. M. Folch, A. Sanchez, D. Babot, L. Varona and M. Perez-Enciso. 2002. Quantitative trait locus mapping for meat quality traits in an Iberian${\times}$Landrace F2 pig population. J. Anim. Sci. 80(11): 2801-2808.
  13. Shin, S. C. and E. R. Chung. 2007. Association of SNP marker in the leptin gene with carcass and meat quality traits in Korean cattle. Asian-Aust. J. Anim. Sci. 20(1):1-6.
  14. Turner, J., H. Nicholas, D. Bishop, J. M. Matthews and M. Crossley. 2003. The LIM protein FHL3 binds basic Kruppellike factor/Kruppel-like factor 3 and its co-repressor Cterminal-binding protein 2. J. Biol. Chem. 278(15):12786-12795. https://doi.org/10.1074/jbc.M300587200
  15. Xiong, Y. Z. and C. Y. Deng. 1999. Principle and method of swine testing. Chinese Agriculture Press, Beijing.
  16. Zuo, B., Y. Z. Xiong, C. Y. Deng, Y. H. Su, J. Wang, M. G. Lei, F. E Li, S. W. Jiang and R. Zheng. 2004. cDNA cloning, genomic structure and polymorphism of the porcine FHL3 gene. Anim. Genet. 3:230-233.