• Title/Summary/Keyword: Infiltration model

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Anti-allergic Effects of Samhwangsasim-tang ($S{\bar{a}}nhu{\acute{a}}ngxi{\grave{e}}x{\bar{i}}nt{\bar{a}}ng$) on Ovalbumin-induced Allergic Model in Mice (삼황사심탕(三黃瀉心湯)이 난황 알부민으로 유도된 알레르기 Mouse모델에서 항알레르기 효과)

  • Choi, Chong-Hwan;Keum, Seon-Oh;Lee, Se-Won;Kim, Il-Hyun;Lee, Ha-Il;Song, Yung-Sun
    • Journal of Korean Medicine Rehabilitation
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    • v.24 no.3
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    • pp.71-85
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    • 2014
  • Objectives In this study, we investigated the inhibitory effects of Samhwangsasim-tang (S.H) on the allergic response caused by ovalbumin(OVA) sensitization and challenge in BALB/c mice. Methods The experimental animals were divided into five groups; 1) normal as negative control, 2) OVA-sensitized mice, 3) OVA-sensitized mice treated with 200 mg/kg of S.H 200, 4) OVA-sensitized mice treated with 400 mg/kg of S.H 400, and 5) OVA-sensitized mice treated with 5 mg/kg of Dexamethasone (Dex). Antigen sensitization for allergic mouse model was performed with twice injection of OVA for 2 weeks. After secondary injection, S.H was administrated orally into mice every day for 13 days and the inhibitory effect of S.H on allergic responses was evaluated. Results Treatment of S.H into allergic mice reduced significantly ear edema and infiltration of immune cells in ear tissues induced with OVA challenge in a dose-dependent manner. S.H reduced significantly the serum levels of Total Immunoglobulin(Ig)G and IgE, and particularly inhibited the production of OVA-specific IgE, but not OVA-specific IgG. The serum level of proinflammatory cytokine TNF-${\alpha}$ and Th2-associated cytokine IL-4 also were significantly decreased by S.H adminstration in a dose denpendent manner. S.H attenuated OVA-induced secretion of IFN-${\gamma}$, but not IL-12 which is a cytokine inducing the development of Th1 cells. It also reduced significantly the secretion of IL-4, which is a cytokine inducing the development of Th2 cells, after splenocytes were stimulated with OVA. However the secretion of IL-5 and IL-13 was influenced weakly or a little. Conclusions These results indicate that S.H could reduce the allergic response through inhibition of antigen-specific IgE and Th2-inducing cytokines. It suggest that S.H may be available clinically for the treatment of allergic patients.

Heat shock protein X purified from Mycobacterium tuberculosis enhances the efficacy of dendritic cells-based immunotherapy for the treatment of allergic asthma

  • Kim, Hye-Young;Kang, Hyun Kyu;Cho, Joon;Jung, In Duk;Yoon, Gun Young;Lee, Min-Goo;Shin, Sung Jae;Park, Won Sun;Park, Jong-Hwan;Ryu, Seung-Wook;Park, Yeong-Min;You, Ji Chang
    • BMB Reports
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    • v.48 no.3
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    • pp.178-183
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    • 2015
  • Dendritic cells play an important role in determining whether na${\ddot{i}}$ve T cells mature into either Th1 or Th2 cells. We determined whether heat-shock protein X (HspX) purified from Mycobacterium tuberculosis regulates the Th1/Th2 immune response in an ovalbumin (OVA)-induced murine model of asthma. HspX increased interferon-gamma, IL-17A, -12 and transforming growth factor (TGF)-${\beta}$ production and T-bet gene expression but reduced IL-13 production and GATA-3 gene expression. HspX also inhibited asthmatic reactions as demonstrated by an increase in the number of eosinophils in bronchoalveolar lavage fluid, inflammatory cell infiltration in lung tissues, airway luminal narrowing, and airway hyper-responsiveness. Furthermore, HspX enhanced OVA-induced decrease of regulatory T cells in the mediastinal lymph nodes. This study provides evidence that HspX plays critical roles in the amelioration of asthmatic inflammation in mice. These findings provide new insights into the immunotherapeutic role of HspX with respect to its effects on a murine model of asthma.

Absorbable Guided Bone Regeneration Membrane Fabricated from Dehydrothermal Treated Porcine Collagen (Dehydrothermal Treatment로 제작한 흡수성 콜라겐 골유도재생술 차단막)

  • Pang, Kang-Mi;Choung, Han-Wool;Kim, Sung-Po;Yang, Eun-Kyung;Kim, Ki-Ho;Kim, Soung-Min;Kim, Myung-Jin;Jahng, Jeong-Won;Lee, Jong-Ho
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.33 no.2
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    • pp.112-119
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    • 2011
  • Purpose: Collagen membranes are used extensively as bioabsorbable barriers in guided bone regeneration. However, collagen has different effects on tissue restoration depending on the type, structure, degree of cross-linking and chemical treatment. The purpose of this study was to evaluate the inflammatory reaction, bone formation, and degradation of dehydrothermal treated porcine type I atelocollagen (CollaGuide$^{(R)}$) compared to of the non-crosslinked porcine type I, III collagen (BioGide$^{(R)}$) and the glutaldehyde cross-linked bovine type I collagen (BioMend$^{(R)}$) in surgically created bone defects in rat mandible. Methods: Bone defect model was based upon 3 mm sized full-thickness transcortical bone defects in the mandibular ramus of Sprague-Dawley rats. The defects were covered bucolingually with CollaGuide$^{(R)}$, BioMend$^{(R)}$, or BioGide$^{(R)}$ (n=12). For control, the defects were not covered by any membrane. Lymphocyte, multinucleated giant cell infiltration, bone formation over the defect area and membrane absorption were evaluated at 4 weeks postimplantation. For comparison of the membrane effect over the bone augmentation, rats received a bone graft plus different covering of membrane. A $3{\times}4$ mm sized block graft was harvested from the mandibular angle and was laid and stabilized with a microscrew on the naturally existing curvature of mandibular inferior border. After 10 weeks postimplantation, same histologic analysis were done. Results: In the defect model at 4 weeks post-implantation, the amount of new bone formed in defects was similar for all types of membrane. Bio-Gide$^{(R)}$ membranes induced significantly greater inflammatory response and membrane resorption than other two membranes; characterized by lymphocytes and multinucleated giant cells. At 10 weeks postoperatively, all membranes were completely resorbed. Conclusion: Dehydrotheramal treated cross-linked collagen was safe and effective in guiding bone regeneration in alveolar ridge defects and bone augmentation in rats, similar to BioGide$^{(R)}$ and BioMend$^{(R)}$, thus, could be clinically useful.

RG-II from Panax ginseng C.A. Meyer suppresses asthmatic reaction

  • Jung, In-Duk;Kim, Hye-Young;Park, Jin-Wook;Lee, Chang-Min;Noh, Kyung-Tae;Kang, Hyun-Kyu;Heo, Deok-Rim;Lee, Su-Jung;Son, Kwang-Hee;Park, Hee-Ju;Shin, Sung-Jae;Park, Jong-Hwan;Ryu, Seung-Wook;Park, Yeong-Min
    • BMB Reports
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    • v.45 no.2
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    • pp.79-84
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    • 2012
  • In asthma, T helper 2 (TH2)-type cytokines such as interleukin (IL)-4, IL-5, and IL-13 are produced by activated $CD^{4+}$ T cells. Dendritic cells played an important role in determining the fate of naive T cells into either $T_H1$ or $T_H2$ cells. We determined whether RG-II regulates the $T_H1/T_H2$ immune response by using an ovalbumin-induced murine model of asthma. RG-II reduced IL-4 production but increased interferon-gamma production, and inhibited GATA-3 gene expression. RG-II also inhibited asthmatic reactions including an increase in the number of eosinophils in bronchoalveolar lavage fluid, an increase in inflammatory cell infiltration in lung tissues, airway luminal narrowing, and airway hyperresponsiveness. This study provides evidence that RG-II plays a critical role in ameliorating the pathogenic process of asthmatic inflammation in mice. These findings provide new insights into the immunotherapeutic role of RG-II in terms of its effects in a murine model of asthma.

Effect of Areca Nut on Helicobacter pylori-Induced Gastric Diseases in Mice

  • Lee, Jinwook;Gunawardhana, Niluka D.;Jang, Sungil;Choi, Yun Hui;Illeperuma, Rasika P.;Kim, Aeryun;Su, Hanfu;Hong, Youngmin A.;Kim, Ji-Hye;Kim, Jinmoon;Jung, Da-Woon;Cha, In-Ho;Bak, Eun-Jung;Cha, Jeong-Heon
    • Journal of Microbiology and Biotechnology
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    • v.26 no.10
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    • pp.1817-1823
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    • 2016
  • Areca nut (AN) chewing is a habit in many countries in Central, Southern, and Southeast Asia. It is strongly associated with the occurrence of oral, pharyngeal, and esophageal cancer as well as systemic inflammation. However, the association between AN intake and the development of gastric lesions has not yet been identified. The aim of this study was to investigate the effect of AN on gastric diseases using a mouse model for Helicobacter pylori infection. We studied four groups of mice: those fed a normal diet (ND), those fed a diet containing 2.5% AN (AD), those fed ND and infected with H. pylori PMSS1 strain (ND/HP), and those fed AD and infected with H. pylori PMSS1 strain (AD/HP). Food intake and body weight were monitored weekly during the experiments. At 10 weeks, the mice were sacrificed, and the stomach weight, H. pylori colonization, and gastric inflammation were evaluated. The stomach weight had increased significantly in the ND/HP and AD/HP groups along with increases in H. pylori colonization; however, there was no significant difference between these two groups with respect to stomach weight and colonization. On histological grading, mononuclear cell infiltration was severer in the AD/HP group than in the ND/HP group. These data suggest that chronic gastric inflammation was aggravated by AN treatment in the mice with H. pylori-induced gastric lesions. Furthermore, as previously suggested, this animal model is useful to determine the effect of potential carcinogens on gastric lesions induced by H. pylori infection.

Fimasartan attenuates renal ischemia-reperfusion injury by modulating inflammation-related apoptosis

  • Cho, Jang-Hee;Choi, Soon-Youn;Ryu, Hye-Myung;Oh, Eun-Joo;Yook, Ju-Min;Ahn, Ji-Sun;Jung, Hee-Yeon;Choi, Ji-Young;Park, Sun-Hee;Kim, Chan-Duck;Kim, Yong-Lim
    • The Korean Journal of Physiology and Pharmacology
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    • v.22 no.6
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    • pp.661-670
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    • 2018
  • Fimasartan, a new angiotensin II receptor antagonist, reduces myocyte damage and stabilizes atherosclerotic plaque through its anti-inflammatory effect in animal studies. We investigated the protective effects of pretreatment with fimasartan on ischemia-reperfusion injury (IRI) in a mouse model of ischemic renal damage. C57BL/6 mice were pretreated with or without 5 (IR-F5) or 10 (IR-F10) mg/kg/day fimasartan for 3 days. Renal ischemia was induced by clamping bilateral renal vascular pedicles for 30 min. Histology, pro-inflammatory cytokines, and apoptosis assays were evaluated 24 h after IRI. Compared to the untreated group, blood urea nitrogen and serum creatinine levels were significantly lower in the IR-F10 group. IR-F10 kidneys showed less tubular necrosis and interstitial fibrosis than untreated kidneys. The expression of F4/80, a macrophage infiltration marker, and tumor necrosis factor $(TNF)-{\alpha}$, decreased in the IR-F10 group. High-dose fimasartan treatment attenuated the upregulation of $TNF-{\alpha}$, interleukin $(IL)-1{\beta}$, and IL-6 in ischemic kidneys. Fewer TUNEL positive cells were observed in IR-F10 compared to control mice. Fimasartan caused a significant decrease in caspase-3 activity and the level of Bax, and increased the Bcl-2 level. Fimasartan preserved renal function and tubular architecture from IRI in a mouse ischemic renal injury model. Fimasartan also attenuated upregulation of inflammatory cytokines and decreased apoptosis of renal tubular cells. Our results suggest that fimasartan inhibited the process of tubular injury by preventing apoptosis induced by the inflammatory pathway.

Dynamic-stability Evaluation of Unsaturated Road Embankments with Different Water Contents (함수비에 따른 불포화 도로성토의 동적 안정성 평가)

  • Lee, Chung-Won;Higo, Yosuke;Oka, Fusao
    • Journal of the Korean Geotechnical Society
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    • v.30 no.6
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    • pp.5-21
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    • 2014
  • It has been pointed out that the collapses of unsaturated road embankments caused by earthquake are attributed to high water content caused by the seepage of the underground water and/or the rainfall infiltration. Hence, it is important to study influences of water content on the dynamic stability and deformation mode of unsaturated road embankments for development of a proper design scheme including an effective reinforcement to prevent severe damage. This study demonstrates dynamic centrifugal model tests with different water contents to investigate the effect of water content on deformation and failure behaviors of unsaturated road embankments. Based on the measurement of displacement, the pore water pressure and the acceleration during dynamic loading, dynamic behavior of the unsaturated road embankments with about optimum water content and the higher water content than the optimum one have been examined. In addition, an image analysis has revealed the displacement field and the distributions of strains in the road embankment, by which deformation mode of the road embankment with higher water content has been clarified. It has been confirmed that in the case of higher water content the settlement of the crown is large mainly owing to the volume compression underneath the crown, while the small confining pressure at the toe and near the slope surface induces large shear deformation with volume expansion.

Research of Runoff Management in Urban Area using Genetic Algorithm (유전자알고리즘을 이용한 도시화 유역에서의 유출 관리 방안 연구)

  • Lee, Beum-Hee
    • Journal of the Korean Geophysical Society
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    • v.9 no.4
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    • pp.321-331
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    • 2006
  • Recently, runoff characteristics of urban area are changing because of the increase of impervious area by rapidly increasing of population and industrialization, urbanization. It needs to extract the accurate topologic and hydrologic parameters of watershed in order to manage water resource efficiently. Thus, this study developed more precise input data and more improved parameter estimating procedures using GIS(Geographic Information System) and GA(Genetic Algorithm). For these purposes, XP-SWMM (EXPert-Storm Water Management Model) was used to simulate the urban runoff. The model was applied to An-Yang stream basin that is a typical Korean urban stream basin with several tributaries. The rules for parameter estimation were composed and applied based on quantity parameters that are investigated through the sensitivity analysis. GA algorithm is composed of these rules and facts. The conditions of urban flows are simulated using the rainfall-runoff data of the study area. The data of area, slope, width of each subcatchment and length, slope of each stream reach were acquired from topographic maps, and imperviousness rate, land use types, infiltration capacities of each subcatchment from land use maps, soil maps using GIS. Also we gave the management scheme of urbanization runoff using XP-SWMM. The parameters are estimated by GA from sensitivity analysis which is performed to analyze the runoff parameters.

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Sensitivity Analysis of the SWMM Model Parameters Based on Design Rainfall Condition (설계강우조건에 따른 SWMM모형 매개변수의 민감도 분석)

  • Lee, Jong-Tae;Hur, Sung-Chul;Kim, Tae-Hwa
    • Journal of Korea Water Resources Association
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    • v.38 no.3 s.152
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    • pp.213-222
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    • 2005
  • This study is a sensitivity analysis of the parameters which affect the simulation results under various design rainfall conditions, using the SWMM model, for three selected basins in urban areas. The sensitivity of the peak flow rate is defined by $S_Q$ (=1.0 - (min. ratio of peak flow rate/max. ratio of peak flow rate)), and the rainfall conditions are classified in terms of design rainfall frequency, duration, and distribution. The simulation results show that in most conditions the parameters - the impermeable area ratio, the sewer slope, and the initial infiltration capacity - have more significant effects on the results than other parameters. As the design rainfall frequency increases, the sensitivity of the sewer slope and sewer roughness increases, while the parameters related with the surface runoff decrease. When the rainfall duration increases, the sensitivities of most parameters of surface runoff and sewer flow decrease. Also, at the 1st quarterly Huff rainfall distribution condition, the impermeable area ratio has high sensitivity, but at the 4th quarterly condition the parameters related with sewer flow show higher sensitivities. These tendencies can be explained by considering the procedure for computing the effective rainfall and kinematic wave on the surface and sewer flow.

Estimation of Saturation Velocity in Soils During Rainfall using Soil Box Test (모형토조실험을 이용한 강우시 토층의 포화속도 산정)

  • Kim, Chul-Min;Song, Young-Suk;Kim, Hak-Joon
    • The Journal of Engineering Geology
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    • v.25 no.3
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    • pp.377-385
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    • 2015
  • We constructed a model test apparatus to evaluate the dependence of the saturation velocity (Vs) in soils on rainfall intensity (IR). The apparatus comprises a soil box, a rainfall simulator, and measuring sensors. The model grounds (60 cm × 50 cm × 15 cm) were formed by Joomunjin standard sand with a relative density of 75%. The rainfall simulator can control the rainfall intensity to reenact the actual rainfall in a soil box. Time Domain Reflectometer (TDR) sensors and tensiometers were installed in the soils to measure changes in the volumetric water content and matric suction due to rainfall infiltration. During the tests, the soil saturation was determined by raising the groundwater table, which was formed at the bottom of the soil box. [Please check that the correct meaning has been maintained.] The wetting front did not form at the ground surface during rainfall because the soil particles were uniform and the coefficient of permeability was relatively high. Our results show that the suction stress of the soils decreased with increasing volumetric water content, and this effect was most pronounced for volumetric water contents of 20%-30%. Based on a regression analysis of the relationship between rainfall intensity and the average saturation velocity, we suggest the following equation for estimating the saturation velocity in soils: Vsavg (cm/sec) = 0.068IR (mm/hr).