Maxillofacial Plastic and Reconstructive Surgery

Korean Association of Maxillofacial Plastic and Reconstructive Surgeons (대한악안면성형재건외과학회)
권호 표지

Absorbable Guided Bone Regeneration Membrane Fabricated from Dehydrothermal Treated Porcine Collagen

Dehydrothermal Treatment로 제작한 흡수성 콜라겐 골유도재생술 차단막

  • Pang, Kang-Mi (Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University) ;
  • Choung, Han-Wool (Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University) ;
  • Kim, Sung-Po (Bioland) ;
  • Yang, Eun-Kyung (Bioland) ;
  • Kim, Ki-Ho (Bioland) ;
  • Kim, Soung-Min (Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University) ;
  • Kim, Myung-Jin (Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University) ;
  • Jahng, Jeong-Won (Dental Research Institute, Seoul National University) ;
  • Lee, Jong-Ho (Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University)
  • 방강미 (서울대학교 치의학대학원 구강악안면외과학교실) ;
  • 정한울 (서울대학교 치의학대학원 구강악안면외과학교실) ;
  • 김성포 (바이오랜드연구소) ;
  • 양은경 (바이오랜드연구소) ;
  • 김기호 (바이오랜드연구소) ;
  • 김성민 (서울대학교 치의학대학원 구강악안면외과학교실) ;
  • 김명진 (서울대학교 치의학대학원 구강악안면외과학교실) ;
  • 장정원 (서울대학교 치의학연구소) ;
  • 이종호 (서울대학교 치의학대학원 구강악안면외과학교실)
  • Received : 2010.09.20
  • Accepted : 2011.01.04
  • Published : 2011.03.31
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Abstract

Purpose: Collagen membranes are used extensively as bioabsorbable barriers in guided bone regeneration. However, collagen has different effects on tissue restoration depending on the type, structure, degree of cross-linking and chemical treatment. The purpose of this study was to evaluate the inflammatory reaction, bone formation, and degradation of dehydrothermal treated porcine type I atelocollagen (CollaGuide$^{(R)}$) compared to of the non-crosslinked porcine type I, III collagen (BioGide$^{(R)}$) and the glutaldehyde cross-linked bovine type I collagen (BioMend$^{(R)}$) in surgically created bone defects in rat mandible. Methods: Bone defect model was based upon 3 mm sized full-thickness transcortical bone defects in the mandibular ramus of Sprague-Dawley rats. The defects were covered bucolingually with CollaGuide$^{(R)}$, BioMend$^{(R)}$, or BioGide$^{(R)}$ (n=12). For control, the defects were not covered by any membrane. Lymphocyte, multinucleated giant cell infiltration, bone formation over the defect area and membrane absorption were evaluated at 4 weeks postimplantation. For comparison of the membrane effect over the bone augmentation, rats received a bone graft plus different covering of membrane. A $3{\times}4$ mm sized block graft was harvested from the mandibular angle and was laid and stabilized with a microscrew on the naturally existing curvature of mandibular inferior border. After 10 weeks postimplantation, same histologic analysis were done. Results: In the defect model at 4 weeks post-implantation, the amount of new bone formed in defects was similar for all types of membrane. Bio-Gide$^{(R)}$ membranes induced significantly greater inflammatory response and membrane resorption than other two membranes; characterized by lymphocytes and multinucleated giant cells. At 10 weeks postoperatively, all membranes were completely resorbed. Conclusion: Dehydrotheramal treated cross-linked collagen was safe and effective in guiding bone regeneration in alveolar ridge defects and bone augmentation in rats, similar to BioGide$^{(R)}$ and BioMend$^{(R)}$, thus, could be clinically useful.

Keywords

References

  1. Dahlin C, Linde A, Gottlow J, Nyman S. Healing of bone defects by guided tissue regeneration. Plast Reconstr Surg 1988;81:672-6. https://doi.org/10.1097/00006534-198805000-00004
  2. Buser D, Dula K, Hess D, Hirt HP, Belser UC. Localized ridge augmentation with autografts and barrier membranes. Periodontol 2000 1999;19:151-63. https://doi.org/10.1111/j.1600-0757.1999.tb00153.x
  3. Gottlow J. Guided tissue regeneration using bioresorbable and non-resorbable devices: initial healing and long-term results. J Periodontol 1993;64(11 Suppl):1157-65. https://doi.org/10.1902/jop.1993.64.11s.1157
  4. Wallace SS, Froum SJ, Cho SC, et al. Sinus augmentation utilizing anorganic bovine bone (Bio-Oss) with absorbable and nonabsorbable membranes placed over the lateral window: histomorphometric and clinical analyses. Int J Periodontics Restorative Dent 2005;25:551-9.
  5. Lim HC, Chae GJ, Jung UW, et al. Initial tissue response of biodegradable membrane in rat subcutaneous model. J Korean Acad Periodontol 2007;37:839-48. https://doi.org/10.5051/jkape.2007.37.4.839
  6. Bunyaratavej P, Wang HL. Collagen membranes: a review. J Periodontol 2001;72;215-29. https://doi.org/10.1902/jop.2001.72.2.215
  7. Friedmann A, Strietzel FP, Maretzki B, Pitaru S, Bernimoulin JP. Histolological assessment of augmented jaw bone utilizing a new collagen barrier membrane compared to a standard barrier membrane to protect a granular bone substitute material. Clin Oral Implants Res 2002;13:587-94. https://doi.org/10.1034/j.1600-0501.2002.130603.x
  8. Tierney CM, Haugh MG, Liedl J, Mulcahy F, Hayes B, O'Brien FJ. The effects of collagen concentration and crosslink density on the biological, structural and mechanical properties of collagen-GAG scaffolds for bone tissue engineering. J Mech Behav Biomed Mater 2009;2:202-9. https://doi.org/10.1016/j.jmbbm.2008.08.007
  9. Oh TJ, Meraw SJ, Lee EJ, Giannobile WV, Wang HL. Comparative analysis of collagen membranes for the treatment of implant dehiscence defects. Clin Oral Implants Res 2003;14:80-90. https://doi.org/10.1034/j.1600-0501.2003.140111.x
  10. Owens KW, Yukna RA. Collagen membrane resorption in dogs: a comparative study. Implant Dent 2001;10:49-58. https://doi.org/10.1097/00008505-200101000-00016
  11. Paul BF, Mellonig JT, Towle HJ 3rd, Gray JL. Use of a collagen barrier to enhance healing in human periodontal furcation defects. Int J Periodontics Restorative Dent 1992;12: 123-31.
  12. Rothamel D, Schwarz F, Sculean A, Herten M, Scherbaum W, Becker J. Biocompatibility of various collagen membranes in cultures of human PDL fibroblasts and human osteoblast- like cells. Clin Oral Implants Res 2004;15:443-9. https://doi.org/10.1111/j.1600-0501.2004.01039.x
  13. Verissimo DM, Leitao RF, Ribeiro RA, et al. Polyanionic collagen membranes for guided tissue regeneration: Effect of progressive glutaraldehyde cross-linking on biocompatibility and degradation. Acta Biomater 2010;6:4011-8. https://doi.org/10.1016/j.actbio.2010.04.012
  14. Moses O, Pitaru S, Artzi Z, Nemcovsky CE. Healing of dehiscence- type defects in implants placed together with different barrier membranes: a comparative clinical study. Clin Oral Implants Res 2005;16:210-9. https://doi.org/10.1111/j.1600-0501.2004.01100.x
  15. Gough JE, Scotchford CA, Downes S. Cytotoxicity of glutaraldehyde crosslinked collagen/poly(vinyl alcohol) films is by the mechanism of apoptosis. J Biomed Mater Res 2002; 61:121-30. https://doi.org/10.1002/jbm.10145
  16. Drexler JW, Powell HM. DHT crosslinking of electrospun collagen. Tissue Eng Part C Methods 2010 [Epub ahead of print]
  17. Cornwell KG, Lei P, Andreadis ST, Pins GD. Crosslinking of discrete self-assembled collagen threads: Effects on mechanical strength and cell-matrix interactions. J Biomed Mater Res A 2007;80:362-71.
  18. Haugh MG, Jaasma MJ, O'Brien FJ. The effect of dehydrothermal treatment on the mechanical and structural properties of collagen-GAG scaffolds. J Biomed Mater Res A 2009;89:363-9.
  19. Tatakis DN, Promsudthi A, Wikesjo UM. Devices for perio dontal regeneration. Periodontol 2000 1999;19:59-73. https://doi.org/10.1111/j.1600-0757.1999.tb00147.x
  20. Sano A, Maeda M, Nagahara S, et al. Atelocollagen for protein and gene delivery. Adv Drug Deliv Rev 2003;55:1651-77. https://doi.org/10.1016/j.addr.2003.08.005
  21. Luten J, van Nostrum CF, De Smedt SC, Hennink WE. Biodegradable polymers as non-viral carriers for plasmid DNA delivery. J Control Release 2008;126:97-110. https://doi.org/10.1016/j.jconrel.2007.10.028