• Journal of Practical Agriculture & Fisheries Research
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• v.25 no.4
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• pp.111-117
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• 2024

Reproductive research such as artificial insemination and embryo transfer is necessary to produce high-quality riding horses. In this study, we investigated the effects of estrus synchronization and ovulation induction methods, which can be considered the basis of artificial insemination in horses, on the hormone concentration and artificial insemination pregnancy rate of mares. For the purpose of synchronization of estrus in horses, Cidr-plus insertion method, Regumate feeding method, and 150mg progesterone + 10mg estradiol mixed administration method were used. In the Cidr-plus insertion method and the Regumate feeding method, the progesterone concentration reached the appropriate level for ovulation induction on the 8th day of administration. The mixed administration method of 150mg progesterone + 10mg estradiol maintained the progesterone concentration at an appropriate level immediately after administration. With the administration of PGF2a and hCG, progesterone concentration decreased rapidly, making ovulation induction possible. As a result of comparing the pregnancy rate between natural estrus and estrus synchronization, the pregnancy rate was found to be higher in estrus synchronization and ovulation induction. From the results of this study, it is insufficient to judge the effect of the pregnancy rate due to the small number of tests, but in terms of usability, estrus synchronization and ovulation induction were useful. Therefore, it is expected to contribute to improving the efficiency of future roadster production.

• Development and Reproduction
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• v.16 no.4
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• pp.271-277
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• 2012

The effects of ovulation induction in ussurian bullhead, Leiocassis ussuriensis, were investigated by treating ussurian bullhead with hCG, LHRHa, GnRHa, ovaprim, and pimozide. hCG was injected to ussurian bullhead at 0.75% NaCl, 5,000, 10,000, 20,000, and 30,000 IU, respectively. The ovulation inducement rates were 100% in 20,000 and 30,000 IU. Fertilization rates were 82.7% and 79.8%. Hatching rates were 59.4% and 57.2%. Ovulation time was between 16-19 hr The concentrations of LHRHa injected were 0.75 NaCl, 50, 100, 200, 300, and $300{\mu}g/kg$. The ovulation inducement rates were 100% in 300 and $400{\mu}g/kg$. Fertilization and hatching rates were 84.9% and 68.4% at $200{\mu}g/kg$. The times to ovulation were between 23 hr and 34 hr. Ovaprim of 0.75% NaCl, 1.0, 1.5, 2.0, 2.5 and 3.0 ml/kg were injected to the abdominal cavity. The ovulation inducement rate was highest at 2.0 and 3.0 ml/kg to 92% and ovulation time was between 27-38 hr. LHRHa concentrations of 0.75% NaCl, 50, 100, 200, 300 and $400{\mu}g$ were injected with pimozide ($1,000{\mu}g$). Ovulation inducement rate was 100% from 200 to 400 IU with pimozide. Ovulation time was 22-36 h. Fertilization and hatching rates were 88.9% and 70.4% in $200{\mu}g/kg$ with pimozide.

• Clinical and Experimental Reproductive Medicine
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• v.12 no.2
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• pp.15-23
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• 1985

Therapy for anovulation represents one of the more gratifying and successful type of infertility management. Despite the introduction of bromocriptine, human menopausal gonadotropin (HMG), and gonadotropin releasing hormone (GnRH), clomiphene citrate remains the mainstay of ovulation induction therapy. There is wide variability in reported rates of ovulation induction (57-91%) and conception (25-43%) following clomiphene therapy. Factors contributing to this variability among different reports are the differences in dosage and duration of therapy, different criteria utilized in selecting patients for clomiphene therapy and different luteal phase parameters of presumptive ovulation. A review of recent experience with clomiphene citrate in the Department of Obstetrics and Gynecology in Seoul National University from January, 1983 to May, 1985 yields the following conclusions: 1. Ovulation rate per total patients treated was, 69.0% 2. Pregnancy rate per total patients treated was 31.7%, and that per total patients ovulated was 45.9%. 3. Ovulation rate at the dosage level up to 150 mg/day (50.3-53.8%) was somewhat higher than that at 200 mg/day or more (33.3-34.6%), and pregnancy rate per total patients treated was comparable at each dosage level. 4. Ovulation rate per total patients ovulated at each dosage level, where ovulation and conception occurred, showed a decreasing tendency as the dosage increased, but pregnancy rate per total patients conceived was comparable except at 200 mg/day. 5. Cumulative pregnancy rate per total patients conceived in each ovulatory cycle was 68.9% in 3 cycles, 88.9% in 4 cycles, and 100% in 6 cycles.

• Journal of Fisheries and Marine Sciences Education
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• v.27 no.4
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• pp.981-989
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• 2015

This study aims to investigate how ovulation induction of sevenband grouper, Epinephelus septemfasciatus varies when treated with different hormonal substances such as ovaprim, pimozide, LHRHa, and HCG. As for LHRHa, we injected it in different concentrations: $50{\mu}g/kg$, $100{\mu}g/kg$, $150{\mu}g/kg$, and $200{\mu}g/kg$. All hormonal substances including LHRHa were injected into back muscles. As a result, ovulation occurred in all sample groups. The sample group injected with HCG released the largest amount of eggs, and the dimeter of eggs and oil globules were also significantly bigger than those of others. Fertilization rate, embryonic survival rate, and hatching rate were the highest in a LHRHa ($100{\mu}g/kg$) +pimozide ($1,000{\mu}g/kg$) group and buoyant rate in a LHRHa group. Ovulation rate was 100% when LHRHa was injected in different concentrations. The largest amount of eggs was released in $200{\mu}g/kg$. The diameter of eggs and oil globules were the biggest in $200{\mu}g/kg$ and the smallest in $50{\mu}g/kg$. Fertilization rate showed no significant difference through all different levels of concentration: 94.6-95.3%. Buoyant rate was the highest in $100{\mu}g/kg$ and embryonic survival rate and hatching rate in $150{\mu}g/kg$. Based on these experimental results, we concluded that LHRHa in $100{\mu}g/kg$ is the most efficient for the ovulation induction of sevenband grouper.

• Clinical and Experimental Reproductive Medicine
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• v.14 no.2
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• pp.101-108
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• 1987

In order to evaluate the consistency of the serum estradiol pattern and response and to determine the influence of number of ovary on them in repeat cycles in the same patient, 57 cycles in 24 patients who underwent in Vitro Fertilization-Embryo Transfer or Gamete Intrafallopian Transfer in Seoul National University Hospital. The patients were stimulated by follicular stimulating hormone and human menopausal gonadotropin and classified as high(${\geqq}$400 pg/ml) and low(<400 pg/ml) response group according to preovulatory estradiol concentration and classified as three estradiol patterns (A,G.B) according to Jones criteria. Seventeen(89.5%) of 19 patients in when a high response to ovulation induction in their first cycle showed a high response. 40%(2/5) of the second ovulation induction cycle were low response in the patient whose first cycle resulted resulted in a low response. Serum estradiol pattern in the first ovulation induction cycle tended to be repeated in the second cycle. Women with high response tended to be more likely to have A and G patterns and less likely to have a cancellation than those with low response. One-ovary patients were at higher risk for inadequate ovulation induction response.

• Development and Reproduction
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• v.16 no.2
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• pp.137-143
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• 2012

The main objective of this study is to investigate the effects of ovulation induction by treating HCG, LHRHa, GnRHa, ovaprim and pimozidein in long snout bullhead, L. longriostris. All hormons were injected into the muscles of back. Concentration of LHRHa to injection were 20, 50, 100, 150 and 200 ${\mu}g/kg$ and same concentration of LHRHa was injected after 24 hour. The ovulation induction rate was 100% and fertilization and hatching rates were 68.4, 45.2, 58.4 and 33.6% in 50 and 100 ${\mu}g/kg$. The times to ovulation were between 28 and 44 h. HCG was injected in long snout bullhead at 5,000, 10,000, 15,000, 20,000 and 25,000 IU/kg. The ovulation induction rate was 50% in 15,000 and 20,000 IU/kg. Fertlilzation and hatching rates were 55.2, 45.6, 52.8 and 44.8%. Ovulation time was between 72~80 h. HCG concentration of 500, 1,000, 2,000 and 4,000 IU/kg were injected with $50{\mu}g$ of LHRHa. Ovulation and hatching rates in 2,000 IU/kg were 75 and 35%. Ovulation time was 28~48 h. Ovaprim of 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mL/kg were injected to the abdominal cavity. The ovulation induction rate was highest at 2.5 mL/kg to 50% and ovulation time was between 66~86 h. LHRHa concentration of 50, 100, 200, 300 and 400 ${\mu}g/kg$ was injected with pimozide (1,000 ${\mu}g/kg$). Ovulation induction rate was 87% at 100 and 200 IU/kg with pimozide. Ovulation time was between 66~86 h.

• Clinical and Experimental Reproductive Medicine
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• v.14 no.2
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• pp.119-126
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• 1987

Exponential rise rate(ERR) of serum estradiol concentrations during active follicular phase was calculated for 49 ovulation induction cycles by human menopausal gonadotropin to know the ovulation induction outcome according to ERR classified into 3 groups with low, moderate and high ERR values(Group I${\leqq}$0.3, 0.3${\leqq}$0.6，Group III>0.6). The results were summarised as follows : 1. No significant difference in the dosage of human menopausal gonadotropin was identified in each group. 2. The mean serum estradiol concentration at the day of human chorionic gonadotropin injection in Group II and Group ill was significantly higher than that in Group I . 3. The mean diameter of leading follicles at the day of human chorionic gonadotropin injection showed no significant difference in each group. 4. No significant difference in the ovulation rate was observed in relation to ERR. How ever, 20% and 20.8% of pregnancy rate in Group I and Group II were achieved while no pregnancy was occurred in Group III. 5. The ovarian hyperstimulation frequency was significantly higher in Group ill that in Group I and Group II. In conclusion, the study suggests that exponential rise rate of serum estradiol is a useful tool in HMG ovulation induction by preventing ovarian hyperstimulation without reducing pregnancy success rate.

• Clinical and Experimental Reproductive Medicine
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• v.35 no.3
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• pp.231-238
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• 2008

Objective: Significant portion of premature ovarian failure patients ovulate intermittently and even can achieve pregnancy. The clinical features and reproductive potentials of Korean women with premature ovarian failure have never been elucidated. The purpose of this study is to assess ovarian follicular growth and ovulatory potential. Methods: Complete medical records of fifteen women with idiopathic premature ovarian failure were reviewed retrospectively. Subjects with more than 6 ultrasonographic scans and 36 months of follow-up period were included. Once the ovarian follicle was found by ultrasonography, the follicular growth was serially monitored. The result of ovulation induction of 6 cycles in 4 patients were also evaluated. Rate of positive ovarian follicle by ultrasound, rate of natural follicular growth and response of ovulation induction were assessed. Results: Among the 15 patients, 6 women (40%) had ovarian follicle in random ultrasonography. But the chance of positive follicle per ultrasonography scan was 7% (8 out of 115 scans). One out of 6 women eventually ovulated during the follow-up scan. In remaining 5 subjects, size of follicles remained unchanged. None of 6 ovulation induction cycles resulted successful ovulation. Conclusion: Incidence of positive ovarian follicle in Korean premature ovarian failure patients is lower than that of Western counterpart. This result, however, could not be directly compared because of the differences of study design. The ovulation rate by medical ovulation induction in this group is 0%, which is worse than the reported success rate of Western studies.

• Journal of Aquaculture
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• v.10 no.4
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• pp.485-486
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• 1997

The effectiveness of intraperitonial injections of human chorionic gonadotrpin(HCG) follwed 4 days later by either HCG, salmon pituitary extract or des-GLY10[D-Ala6] LHRHa-ethylamide on ovulation of the coho salmon, Oncorhynchus kisutch, were incestigated and the results were discussed.

• Clinical and Experimental Reproductive Medicine
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• v.28 no.4
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• pp.255-264
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• 2001

Objective : This study was performed to investigate the effect of metformin therapy on ovulation induction & pregnancy rate in clomiphene citrate-resistant PCOS women. Method: This study used a randomized, single-blinded, case-controlled methods. Total study group consisted of 21 women who showed clomiphene citrate-resistant parttern on previous ovulation induction cycles. Patients of metformin group received metformin 500 mg three times daily, for 7 weeks. Control group received none. Metformin group was consisted of 10 women and control group was consisted of 11 women. Then clomiphene was administrated at daily 50 mg for 5 days to both groups. Clomiphene dosage was increased to daily 150 mg until ovulation was occurred. Before and After metformin treatment, blood samples for measurement of insulin, glucose, steroids were obtained. Results: In the metformin and control groups, 6 of 10 women (60%) and 2 of 11 women (18%) ovulated. And 4 of 10 women (40%) and 0 of 11 women (0%) conceived. Comparisons between the groups were significant. Conclusion: In PCOS women who are resistant to CC, metformin use increased the ovulation rate and pregnancy rate from CC treatment, significantly.