Journal of Practical Agriculture & Fisheries Research (현장농수산연구지)

Korea National University of Agriculture and Fisheries (국립한국농수산대학교 교육개발센터)
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Effects of Estrus Synchronization and Ovulation Induction Methods on Hormone Concentrations and Pregnancy Rate in Artificial Insemination of Riding Horses

말 인공수정에서 발정동기화와 배란유도 방법이 호르몬 농도와 임신율에 미치는 효과

  • Su heon Gwon (Animal material engineering, Division Animal Science, Gyeongsang National University) ;
  • Yong Soo Park (Department of Livestock, Korea National University of Agriculture and Fisheries)
  • 권수현 (경상대학교 축산과학부) ;
  • 박용수 (한국농수산대학교 축산학부)
  • Received : 2023.12.06
  • Accepted : 2023.12.22
  • Published : 2024.01.20
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Abstract

Reproductive research such as artificial insemination and embryo transfer is necessary to produce high-quality riding horses. In this study, we investigated the effects of estrus synchronization and ovulation induction methods, which can be considered the basis of artificial insemination in horses, on the hormone concentration and artificial insemination pregnancy rate of mares. For the purpose of synchronization of estrus in horses, Cidr-plus insertion method, Regumate feeding method, and 150mg progesterone + 10mg estradiol mixed administration method were used. In the Cidr-plus insertion method and the Regumate feeding method, the progesterone concentration reached the appropriate level for ovulation induction on the 8th day of administration. The mixed administration method of 150mg progesterone + 10mg estradiol maintained the progesterone concentration at an appropriate level immediately after administration. With the administration of PGF2a and hCG, progesterone concentration decreased rapidly, making ovulation induction possible. As a result of comparing the pregnancy rate between natural estrus and estrus synchronization, the pregnancy rate was found to be higher in estrus synchronization and ovulation induction. From the results of this study, it is insufficient to judge the effect of the pregnancy rate due to the small number of tests, but in terms of usability, estrus synchronization and ovulation induction were useful. Therefore, it is expected to contribute to improving the efficiency of future roadster production.

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References

  1. Backman T, Bruemmer JE, Graham JK and Squires EL. 2004. Pregnancy rates of mares inseminated with semen cooled for 18hours and then frozen. J. Anim. Sci. 82:690-694.  https://doi.org/10.2527/2004.823690x
  2. Barbacini, S.; Loomis, P.; Squires, E.L. The eect of sperm number and frequency of insemination on pregnancy rates of mares inseminated with frozenthawed spermatozoa. Anim. Reprod. Sci. 2005, 89, 203-235. 
  3. Bedford-Guaus SJ. 2007. Transported stallion semen and breeding mares with cooled or frozen-thawed semen. Clin. Tech. Equine Pract. 6:239-248.  https://doi.org/10.1053/j.ctep.2007.09.003
  4. Hemberg, E.; Lundeheim, N.; Einarsson, S. Successful timing of ovulation using deslorelin (Ovuplant) is labour-saving in mares aimed for single AI with frozen semen. Reprod. Domest. Anim. 2006, 41, 535-537.  https://doi.org/10.1111/j.1439-0531.2006.00709.x
  5. Loomis PR. 2001. The equine frozen semen industry. Anim. Reprod. Sci. 68:191-200.  https://doi.org/10.1016/S0378-4320(01)00156-7
  6. Loomis PR and Graham JK. 2008. Commercial semen freezing: individual male variation in cryosurvival and the response of stallion sperm to customized freezing protocols. Anim. Reprod. Sci. 105:119-128.  https://doi.org/10.1016/j.anireprosci.2007.11.010
  7. Metcalf, E.S. Optimizing pregnancy rates using frozenthawed equine semen. Anim. Reprod. Sci. 2005, 89, 209-212. 
  8. Morris, L.H.; Tiplady, C.; Allen, W.R. Pregnancy rates in mares after a single fixed time hysteroscopic insemination of low numbers of frozenthawed spermatozoa onto the uterotubal junction. Equine. Vet. J. 2003, 35, 197201. 
  9. Nielsen JM, Kofoed Bock TS and Ersboll AK. 2008. Factors associated with fertility in horse in a Danish equine practice after artificial insemination with frozen-thawed semen. Anim. Reprod. Sci. Suppl. 107:336-337.  https://doi.org/10.1016/j.anireprosci.2008.05.113
  10. Park yong-soo and Cho gil-jae. 2011. Factors affecting on the motility of semen and the pregnancy rate of artificial insemination in equine. J. Emb. Trans. 26:15-19. 
  11. Samper JC. 1995. Stallion semen cryopreservation: factors affecting pregnancy rates. Proc. Soc. Theriogenology 160-165. 
  12. Samper JC. 2008. Induction of estrus and ovulation: Why some mares respond and others do not. Theriogenology 70:445-447.  https://doi.org/10.1016/j.theriogenology.2008.04.040
  13. Sieme H, Schafer T, Stout TA, Klug E and Waberski D. 2003. The effects of different insemination regimes on fertility in mares. Theriogenology 60:1153-1164.  https://doi.org/10.1016/S0093-691X(03)00113-4
  14. Vidament M. 2005. French field results(1985-2005) on factors affecting fertility of frozen stallion semen. Anim. Reprod. Sci. 89:115-136.  https://doi.org/10.1016/j.anireprosci.2005.07.003
  15. Vidament M, Dupere AM, Julienne P, Evain A, Noue P and Palmer E. 1997. Equine frozen semen freezability and fertility results. Theriogenology 48:907-917.  https://doi.org/10.1016/S0093-691X(97)00319-1