• Journal of Plant Biology
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• 제20권2호
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• pp.91-101
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• 1977

Using barley seeds (Hordeum sativum Jess, var.), the influences of gibberellic acid (GA) and indole-3-acetic acid(IAA) on the amylase synthesis and that of the nucleic acid metabolism were investigated. 1. With the deembrynized barley seeds, the increase of amylase treated with a $10^{-5}M$ of GA and the decrease of amylase treated with $10^{-5}M$IAA were matched by a proportionate increase and decrease in the amount of RNA. The influence of the hormones on the RNA synthesis has appeared immediately after the treatment but on the amylase synthesis it has appeared 8 hours later. But no influence on the DNA synthesis was observed on both hormones. 2. The amylase from deembryonized barley seeds treated with GA and IAA have been fractionated by gel filteration on Sephadex G-100. The amylase components showed four fractions on both enzymes treated with GA and IAA. Fraction I(FI) was differed from fraction Ⅵ(FIV) in Km value and the effects of temperature, pH and metal ions. On the basis of their emzymatic properties, it was considered that the FI was $\beta$-amylase and FIV was $\alpha$-amylase. The influences of GA and IAA on each fractions appeared to be similar but on the amylase units per souble protein, IAA inhibited the production of amylase FIV while it promoted that of amylase FI. 3. An experiment was conducted to determine whether IAA inhibits GA-promoted amylase synthesis competitively or non-competitively. Using a Lineweaver-Burk plot, it was clear that IAA was acting in a non-competitive fashion. From this, IAA was probably not competing with GA at the same site, but it was acting at some other site which resutled in partial blocking of the action of GA on the amylase synthesis.

• Applied Biological Chemistry
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• 제32권2호
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• pp.162-169
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• 1989

완두(豌豆) 유묘(幼苗)줄기의 추출물(抽出物)에서 silica gel column chromatography, TLC, HPLC 등으로 분리(分離)하고 여러 가지 정색반응(呈色反應)을 행한 결과 tryptophan, indole-3-acetaldehyde, IAA, indole-3-aldehyde를 확인(確認)할 수 있었다. 여기서 확인(確認)된 indole화합물(化合物)들을 근거(根據)로 볼 때 완두유묘(豌豆幼苗)에는 tryptophan${\rightarrow}$(?)${\rightarrow}$indole-3-acetaldehyde${\rightarrow}$IAA${\rightarrow}$indole-3-aldehyde로 이어지는 대사계(代謝系)가 존재(存在)할 수 있음을 추정(推定)할 수 있었다. 한편, IAA-당(糖) 복합체(複合體)로써 IAA-rhamnose와 IAA-glucose가 완두 유묘에 분포함을 잠정적으로 확인하였다.

• 미생물학회지
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• 제10권3호
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• pp.117-127
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• 1972

To study the effect of IAA on the growth of Chlorella, the alage wre cultured on the media for six days by bubbling $_{2}$ enriched air under 10K lux at 20-$25^{\circ}C$. The culture media were made by adding a concentration of $10^{-3}$M, $10^{-4}$M, and 0M(as a control) IAA to the standard media. During the period of culture, Chlorella was smapled for the given time of interval and photosynthetic and respiratory activities were measured by Warburg manometer and change of chemical components of Chlorella was determined by spectrophotometry after the Chlorella cell was fractionated by Schmidt-Thannhauser method. 1) Photosynthetic and respiratory activities were enhanced by IAA ; especially the enhancement of respiratory activity was so remarkable. 2) As to the chemical components of Chlorella, carbohydrates and amino acids were reduced a little but phosphate, RNA, DNA, and protein were increased by $10^{-3}$M IAA ; the increase of RNA, in particular, was noticable. 3) The above results suggest that the enhancement of growth of Chlorella, by IAA and ATP induced by respiratory activity accelerated with IAA enhanced RNA synthesis, resulting in an increase of protein synthesis.

• 한국토양비료학회지
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• 제43권1호
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• pp.96-104
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• 2010

무균 및 온실조건에서 indole-3-acetic acid (IAA)의 처리와 1-aminocyclopropane-1-carboxylate deaminase (ACCD) 및 IAA 활성을 갖는 Methylobacterium 균주 접종 시 토마토와 고추의 생장을 비교 평가하였다. 무균조건에서 1.0 ${\mu}g\;mL^{-1}$의 IAA는 고추와 토마토의 뿌리생장을 촉진시키는데 비해 10.0 ${\mu}g\;mL^{-1}$ 이상의 높은 농도에서는 뿌리생장이 억제되었다. 그러나 높은 ACCD 활성을 갖고, IAA 활성은 낮거나 가지고 있지 않은 Methylobacterium 균주들을 접종하였을 때에는 고추와 토마토 모두 IAA 처리구 보다 뿌리생장이 증진되는 것을 확인하였다. 마찬가지로 온실조건에서 Methylobacterium 균주들을 접종했을 때, 마디길이와 잎의 크기 그리고 단위 면적당 잎의 무게 (SLW)에서 유의성 있는 증진효과를 보였다. 전반적인 식물 생장에서 저농도의 IAA 처리 효과는 Methylobacterium의 효과와 비슷한 경향을 나타냈다. 유묘의 지상부 길이는 ACCD 활성과 IAA 생산능을 갖는 Methylobacterium 균주 처리구에서 유의성 있는 증가를 확인할 수 있었으며, 전체 건물중 또한 Methylobacterium 처리 시 유의성 있는 증진 효과를 확인 할 수 있었다. 하지만 고농도의 IAA는 고추와 토마토의 생물량을 억제시켰다. 이러한 결과는 접종 균주의 IAA와 ACCD가 고추와 토마토 유묘의 생장 증진에 영향을 끼친다는 것을 증명한다.

• Journal of Microbiology and Biotechnology
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• 제18권7호
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• pp.1235-1244
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• 2008

Indole-3-acetic acid (IAA) is produced commonly by plants and many bacteria, however, little is known about the genetic basis involving the key enzymes of IAA biosynthetic pathways from Bacillus spp. IAA intermediates from the Gram-positive spore-forming bacterium Paenibacillus polymyxa E681 were investigated, which showed the existence of only an indole-3-pyruvic acid (IPA) pathway for IAA biosynthesis from the bacterium. Four open reading frames (ORFs) encoding indole-3-pyruvate decarboxylase-like proteins and putative indole-3-pyruvate decarboxylase (IPDC), a key enzyme in the IPA synthetic pathway, were found on the genome sequence database of P. polymyxa and cloned in Escherichia coli DH5$\alpha$. One of the ORFs, PP2_01257, was assigned as probable indole-3-pyruvate decarboxylase. The ORF consisted of 1,743 nucleotides encoding 581 amino acids with a deduced molecular mass of 63,380 Da. Alignment studies of the deduced amino acid sequence of the ORF with known IPDC sequences revealed conservation of several amino acids in PP2_01257, essential for substrate and cofactor binding. Recombinant protein, gene product of the ORF PP2_01257 from P. polymyxa E681, was expressed in E. coli BL21 (DE3) as a glutathione S-transferase (GST)-fusion protein and purified to homogeneity using affinity chromatography. The molecular mass of the purified enzyme showed about 63 kDa, corresponding closely to the expected molecular mass of IPDC. The indole-3-pyruvate decarboxylase activity of the recombinant protein, detected by HPLC, using IPA substrate in the enzyme reaction confirmed the identity and functionality of the enzyme IPDC from the E681 strain.

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book I
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• pp.617-629
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• 2003

A recent development in cosmetics has been the pursuit of wrinkling in the skin. The cosmetics composed of anti-wrinkle agent stand out from the point of view of environmental contamination and pollution. Among them, Indole-3-acetic acid (IAA), studied with wrinkling pigmentation and swelling conditions in the area of the eye, showed clinically significant reduction in depth of lines during one month trial using skin treatment. But, IAA has shown some problems when used in cosmetic formulations, such as stability, permeability and toxicity. The results of the clinical examination were shown that its permeability and toxicity didn't matter. To increase the stability of IAA, antioxidants such as Licorice, ubiquinone, tocopherol, Baicalin, ferulic acid, BHT, ascorbic acid, sodium metabisulfite, and so on were employed in cosmetic formulations. Our main purpose is the study for the stability efficiency and effect of each other of cream formulations containing optimal dosage antioxidants (o/w type emulsion), This study evaluated wrinkle reduction effect of IAA, which is used in cosmetics.

• 한국토양비료학회지
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• 제39권6호
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• pp.386-391
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• 2006

본 연구는 생물학적 토양건전성평가 지표로서의 토양의 탈수소효소 생성균 밀도와 IAA생성능의 적용 가능성을 살펴보고자, 무시용구, 질소 단용구, 3요소 시용구 및 퇴비 3요소 시용구로 처리된 논토양에서의 탈수소효소 생성균 및 토양의 IAA 생성능의 변화에 대해 조사하였다. 비색법과 HPLC법 간의 IAA분석치는 유의성 있는 정의 상관관계가 있었으며, 시험토양의 IAA생성량은 비색법 $0.83{\sim}1.23{\mu}g\;IAA\;g^{-1}h^{-1}$, HPLC법 $0.80{\sim}1.29{\mu}g\;IAA\;g^{-1}h^{-1}$로 분석법 간에 거의 비슷한 수준을 보였다. 탈수소효소생성 토양세균과 토양의 IAA생성능은 대조구와 N 단용구에 비해 NPK구 및 CNPK구에서 유의적으로 높은 결과를 보였으며, 탈수소효소 생성세균과 토양의 IAA 생성능 간에는 유의성이 높은 정의 상관관계를 보였다.

• 한국미생물·생명공학회지
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• 제18권4호
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• pp.327-330
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• 1990

Thalictrum rugosum 세포배양에서 여러 가지 식물생장 조절물질이 세포증식 및 berberine 생산에 미치는 영향을 조사하였다. 조사된 식물생장 조절물질들 중에서 indole-3-acetic acid(IAA)가 berberine 생산에 가장 적합함을 알 수 있었고 그 최적농도는 1$\mu \textrm m$ 이었다. 비교 기준인 2,4-D의 사용결과에 비할 때 60이상의 생산량 상승효과가 있었으며, cytokinin인 6-benzylaminopurine(BA)를 동시에 사용하는 것보다 IAA만을 단독으로 사용하는 것이 더 효과적이다.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1995년도 식물학심포지움 식물로부터 유용 2차대사산물의 생산 PRODUCTION OF USEFUL SECONDARY METABOLITES FROM PLANTS
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• pp.12-26
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• 1995

• 한국표면공학회지
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• 제57권4호
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• pp.265-273
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• 2024

An amperometric sensor for measuring indole-3-acetic acid (IAA) was studied based on a screen-printed carbon electrode (SPCE) coated with a reduced graphene oxide composite electrocatalyst. The PEI-GO dispersion is uniformly formed through a nucleophilic substitution reaction between the active amine group of Polyethyleneimine (PEI) and the epoxide group exposed on the surface of graphene oxide. And The 3-dimensional PEI-rGO AG (Polyethyleneimine-reduced graphene oxide aerogel) complex was easily prepared through simple heat treatment of the combined PEI-GO dispersion. The proposed composite catalyst electrode, PEI-rGO AG/SPCE, showed a two linear relationship in the low and high concentrations in IAA detection, and the linear equation was I_pa = 0.2883C + 0.0883 (R²=0.9230) at low concentration and Ipa = 0.00464C + 0.6623 (R²=0.9894) at high concentration was proposed, and the detection limit was calculated to be 203.5nM±33.2nM. These results showed the applicability of the PEI-rGO AG composite catalyst as an electrode material for electrocatalysts for the detection of IAA.