• Title/Summary/Keyword: Index of Shape

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Isolation and Characterization of mas1+ of Schizosaccharomyces pombe, a Homologue of Human CIP29/Hcc-1 Involved in the Regulation of Cell Division (세포분열에 관여하는 인간의 CIP29/Hcc1 유전자와 상동성을 가지는 분열형 효모의 새로운 유전자 mas1+의 특성분석)

  • Cha, Jae-Young;Shin, Sang-Min;Ha, Se-Eun;Lee, Jung-Sup;Park, Jong-Kun
    • Journal of Life Science
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    • v.21 no.12
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    • pp.1666-1677
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    • 2011
  • The regulation of gene expression plays an important role in cell cycle controls. In this study, a novel gene, the $mas1^+$($\underline{mi}$tosis $\underline{as}$sociated protein) gene, a homolog of human CIP29/Hcc1, was isolated and characterized from fission yeast Schizosaccharomyces pombe (S. pombe) using a gene-specific polymerase chain reaction. The isolated gene contained a complete open reading frame capable of encoding 245 amino acid residues with a typical promoter, as judged by nucleotide sequence analysis. It was also found that a PCB ($\underline{p}$ombe cell $\underline{c}$ycle $\underline{b}$ox) is located in the promoter region, which controls M-$G_1$ specific transcription in S. pombe. The quantitative analysis of the $mas1^+$ transcript against $adh1^+$ showed that the pattern of expression is similar to that of the septation index. Cytokinesis of mas1 mutant was greatly delayed at $25^{\circ}C$ and $36^{\circ}C$, and a large number of multi-septate cells were produced. The mas1 mutant had 2C, 4C and 6C DNA contents, as determined by FACS analysis. In addition, the number of multi-septate cells significantly increased. When cells were cultured in nitrogen starvation medium to increase proliferation, the abnormal phenotypes of mas1 mutant dramatically increased. These phenotypes could be rescued by an overexpression of the $mas1^+$ gene. The mas1 protein localized in the nuclei of S. pombe and human HeLa cells, as evidenced by Mas1-EGFP signals. The abnormal growth pattern and the morphology of mas1 mutant were complemented by a plasmid carrying human CIP29/Hcc-1cDNA. In addition, CIP29 /Hcc-1 transcript level increased in active cell proliferation stages in the developing mouse embryos. These results indicate that the $mas1^+$ ishomologous to the human CIP29/Hcc1 gene and is involved in cytokinesis and cell shape control.

Development of Greenhouse Cooling and Heating Load Calculation Program Based on Mobile (모바일 기반 온실 냉난방 부하 산정 프로그램 개발)

  • Moon, Jong Pil;Bang, Ji Woong;Hwang, Jeongsu;Jang, Jae Kyung;Yun, Sung Wook
    • Journal of Bio-Environment Control
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    • v.30 no.4
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    • pp.419-428
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    • 2021
  • In order to develope a mobile-based greenhouse energy calculation program, firstly, the overall thermal transmittance of 10 types of major covers and 16 types of insulation materials were measured. In addition, to estimate the overall thermal transmittance when the cover and insulation materials were installed in double or triple layers, 24 combinations of double installations and 59 combinations of triple installations were measured using the hotbox. Also, the overall thermal transmittance value for a single material and the thermal resistance value were used to calculate the overall thermal transmittance value at the time of multi-layer installation of covering and insulating materials, and the linear regression equation was derived to correct the error with the measured values. As a result of developing the model for estimating thermal transmittance when installing multiple layers of coverings and insulating materials based on the value of overall thermal transmittance of a single-material, the model evaluation index was 0.90 (good when it is 0.5 or more), indicating that the estimated value was very close to the actual value. In addition, as a result of the on-site test, it was evaluated that the estimated heat saving rate was smaller than the actual value with a relative error of 2%. Based on these results, a mobile-based greenhouse energy calculation program was developed that was implemented as an HTML5 standard web-based mobile web application and was designed to work with various mobile device and PC browsers with N-Screen support. It had functions to provides the overall thermal transmittance(heating load coefficient) for each combination of greenhouse coverings and thermal insulation materials and to evaluate the energy consumption during a specific period of the target greenhouse. It was estimated that an energy-saving greenhouse design would be possible with the optimal selection of coverings and insulation materials according to the region and shape of the greenhouse.

Derivation of Stem Taper Equations and a Stem Volume Table for Quercus acuta in a Warm Temperate Region (난대지역 붉가시나무의 수간곡선식 도출 및 수간재적표 작성)

  • Suyoung Jung;Kwangsoo Lee;Hyunsoo Kim; Joonhyung Park;Jaeyeop Kim;Chunhee Park;Yeongmo Son
    • Journal of Korean Society of Forest Science
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    • v.112 no.4
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    • pp.417-425
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    • 2023
  • The aim of this study was to derive stem taper equations for Quercus acuta, one of main evergreen broad-leaved tree species found in warm temperate regions, and to prepare a stem volume table using those stem taper equations. A total of 688 individual trees were used in the analysis, which were collected from Jeonnam-do, Gyeongnam-do, and Jeju-do. The stem taper models applied to derive the stem curve pattern were the Max and Burkhart, Kozak, and Lee models. Among the three stem taper models, the best explanation of the stem curve shape of Q. acuta was found to be given by the Kozak model, which showed a fitness index of 0.9583, bias of 0.0352, percentage of estimated standard error of 1.1439, and mean absolute deviation of 0.6751. Thus, the stem taper of Q. acuta was estimated using the Kozak model. Moreover,thestemvolumecalculationwasperforme d by applying the Smalian formula to the diameter and height of each stem interval. In addition, an analysis of variance (ANOVA) was conducted to compare the two existing Q. acuta stem volume tables (2007 and 2010) and the newly created stem volume table (2023). This analysis revealed that the stem volume table constructed in the Wando region in 2007 included about twice as much as the stem volume tables constructed in 2010 and 2023. The stem volume table (2023) developed in this study is not only based on the regional collection range and number of utilized trees but also on a sound scientific basis. Therefore, it can be used at the national level as an official stem volume table for Q. acuta.

Efficiency of ceramic bracket debonding with the Er:YAG laser (세라믹 브라켓의 제거 시 Er:YAG 레이저의 효능)

  • Suh, Chung-Hwan;Chang, Na-Young;Chae, Jong-Moon;Cho, Jin-Hyoung;Kim, Sang-Cheol;Kang, Kyung-Hwa
    • The korean journal of orthodontics
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    • v.39 no.4
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    • pp.213-224
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    • 2009
  • Objective: The aim of this study was to find out whether Er:YAG laser can aid in debonding ceramic brackets, and to see what kind of method will be the most appropriate for debonding. Methods: One hundred and ninety teeth, monocrystalline brackets ($MISO^{TM}$, HT, Ansan-Si, Korea), polycrystalline brackets ($Transcend^{TM}$ series 6000, 3M Untek, Monrovia, CA, USA) and the KEY Laser3 (KavoDental, Biberach, Germany) were used. Experimental groups were classified according to the type of ceramic brackets, and the amount of laser energy (0, 140, 300, 450, 600 mJ). After applying laser on the bracket at two points at 1 pulse each, the shear bond strength was measured. The effect of heat caused by laser was measured at the enamel beneath the bracket and pulp chamber. After measuring the shear bond strength, adhesive residue was evaluated and enamel surface was investigated using SEM. Results: All ceramic bracket groups showed a significant decrease in shear bond strength as the laser energy increased. The greatest average temperature change was $3.78^{\circ}C$ on the enamel beneath the bracket and $0.9^{\circ}C$ on the pulp chamber. Through SEM, crater shape holes caused by the laser was seen on the enamel and adhesive surfaces. Conclusions: If laser is applied on ceramic brackets for debonding, 300 - 450 mJ of laser energy will be safe and efficient for monocrystalline brackets ($MISO^{TM}$), and about 450 mJ for polycrystalline brackets ($Transcend^{TM}$ series 6000).

Comparison of Flavonoid Contents and Antioxidant Activity of Yuzu (Citrus junos Sieb. ex Tanaka) Based on Harvest Time (산지별 유자의 수확시기에 따른 플라보노이드 함량 및 항산화활성 비교)

  • Moon, So Hyun;Assefa, Awraris Derbie;Ko, Eun Young;Park, Se Won
    • Horticultural Science & Technology
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    • v.33 no.2
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    • pp.283-291
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    • 2015
  • The aim of this study was to evaluate the changes of fruit quality, flavonoid contents and antioxidant activity of Yuzu (Citrus junos Sieb. ex Tanaka) from Go-heung and Jeju according to harvest time. Samples were harvested from August to December on the $1^{st}$ of every month. August and September samples were green colored, whereas November and December samples were yellow. The fruit shape index decreased, changing from globular to elliptical, whereas the $^{\circ}birx$ increased with ripening stage. The yuzu from Jeju was larger than that from Go-heung in each month of cultivation. August samples exhibited the highest amounts of phenolic compounds. In addition, samples from Jeju had higher total phenolic content than those from Go-heung. The content of phenolic compounds decreased with ripening until October and then increased subsequently. Antioxidant activity of the yuzu was evaluated by FRAP and DPPH methods. The antioxidant activity showed a similar trend as total phenolic content. Immature yuzu fruit was found to exhibit the highest amount of flavonoids such as naringin and hesperidin. November and December samples showed almost the same contents of flavonoids. The flavonoid content of yuzu fruit harvested from Jeju was higher than that from Go-heung. Overall, the samples harvested at the early stage, in the month of August, exhibited the highest flavonoid content, phenolic content and antioxidant activity. As the health benefits of these compounds has been demonstrated in various studies, the immature yuzu appears to be preferable for use as a raw material for formulation of pharmaceutical products as well as for functional food production after a proper in-vivo and in-vitro medical tests.

A MORPHOMETRIC STUDY OF THE MAXILLARY PRIMARY FIRST MOLARS USING THREE-DIMENSIONAL SCANNER (3차원 스캐너를 이용한 상악 제1유구치 치관의 크기와 형태에 관한 연구)

  • Lee, Jong-Beom;Kim, Chong-Chul;Hahn, Se-Hyun;Lee, Sang-Hoon
    • Journal of the korean academy of Pediatric Dentistry
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    • v.33 no.4
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    • pp.643-652
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    • 2006
  • The purpose of this study was to elucidate the size and morphologic characteristics of maxillary primary first molars in Korean children using three-dimensional laser scanner and compare three-dimensional image with preformed stainless steel crown. Scanned three-dimensional images of dental cast taken from 132 children(male 62, female 70) by three-dimensional laser scanner(Breuckmann opto-Top HE100, INUS, Korea) were used. Mesiodistal diameter, buccolingual diameter, occlusogingival height and crown shape of each image were calculated by Rapidform 2004 program(INUS, Korea). The values were statistically compared by independent samples t-test with 95% of significant level. The results were as follows : 1. No significant difference in crown size was found between left and right maxillay primary first molar(p>0.05). 2, Significant difference in mesiodistal diameter, buccolingual diameter, buccal occlusogingival height was found between male and female (p<0.05), and crown size of male was bigger than that of female. 3. Average image of maxillay primary first molar was shaped three-dimensionally and measured. In comparison with 3M stainless steel crown, this image was similar with No.4 or No.5 SS crown in male, No.4 in female. In comparison with ILSUNG SS crown, this image was similar with No.5 in male, No.4 in female. 4 Mesiolingual line angle area, distolingual line angle area and buccogingival ridge were more obvious in average image than 3M stainless steel crown. ILSUNG SS crown was more square and had longer mesiodistal diameter than average 3D image.

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Physical Characteristics of Seasoning Pork during Aging 8t Cold Temperature (저온 숙성에 의한 양념 돈육의 물리적 특성)

  • Hah Kyung-Hee;Ahn Chong-Nam;Joo Seon-Tea;Park Gu-Boo;Sung Nak-Ju;Park Ki-Hoon;Kim Il-Suk;Jin Sang-Keun;Chung Ku-Young
    • Food Science of Animal Resources
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    • v.25 no.4
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    • pp.397-402
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    • 2005
  • This study was carried out to evaluate the physical characteristics of seasoned pork using low fat loin portion. The samples, pork loins were cut by the shape of cube $(5\times15\times5cm)$ and seasoned pork with Korean traditional sauces such as soy sauce base (T1), red pepper sauce base (T2), and soybean sauce base (T3) in the same proportion of meat seasonings, respectively. The seasoned samples were aging at $1\pm1^{\circ}C$ for 28 days. pH of seasoned pork with soybean sauce and red pepper sauce were higher compared to seasoned pork with soy sauce at 1 day of aging and pH of all treatments were not much changed during aging periods. Shear force was decreased in seasoned pore with soybean sauce and red pepper sauce, however seasoned pork with soy sauce was increased during aging periods. Water holding capacity (WHC) was decreased in seasoned pork with soy sauce and red pepper sauce, but seasoned pork with soybean sauce was maintained during aging periods. Cooking loss of seasoned pork with soy sauce was higher than other treatment. Myofibrillar fragmentation index (MFI) or all treatment was increased during in Periods (T3>T2>T1). Tenderness or seasoned pork could be improved by the soybean sauce and red pepper sauce. Both soybean and red pepper sauce were decreased shear lone and increased MFI and WHC of seasoned pork Tenderness of seasoned pork were improved by the soybean sauce and red pepper sauce.

A Study on the Bird Communities and Similarity of Three Streams in Daejeon Metropolitan City (대전 3대 하천의 조류군집과 유사성에 관한 연구)

  • Kim, In-Kyu;Lee, Han-Soo;Paek, Woon-Kee;Lee, Joon-Woo
    • Korean Journal of Environment and Ecology
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    • v.24 no.2
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    • pp.147-156
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    • 2010
  • This study was conducted from April, 2002 to March 2006, using three urban streams(Gap Stream, Yudeung Stream and Daejeon Stream) in Daejeon Metropolitan City. 12,027 individual birds summed by the peak count in 126 species, 34 families, and 13 orders were observed from three stream sites. Dominant species were of Anas poecilorhyncha, Anas crecca, Columba livia, Passer montanus, and Egretta garzetta(in that order). The groups of birds were classified into six types. The most frequent group were the arbor birds(54 species), while the smallest group was the diving ducks(7 species). As for the number of individuals, the shrub bird group had 721 individuals while the dabbling ducks observed had 4,974 individuals. Regarding the distribution of birds appeareing in each stream, 14,885 individual numbers in 114 species were observed at Gap Stream, 6,642 individuals in 90 species at Yudeung Stream and 4,202 individuals in 69 species at Daejeon Stream. Various indices of the birds were analyzed with respects to the similarities between streams. Gap Stream had similar characteristics to Yudeung Stream, and the latter was similar to Daejeon Stream. However, Gap Stream and Daejeon Stream showed different characteristics. The dominance index of each section was calculated using ten dominant bird species top-down. Subsequently, the birds and their preferred environment were analyzed. The results showed that shrub birds and arbor birds preferred the upper stream of every stream, while herons and dabbling ducks inhabited the midstream. Dabbling ducks and some diving ducks appeared downstream.

Effects of Dense Planting on the Growth and Producivity in Hot Pepper(Capsicum annum L.) (재식밀도(裁植密度)가 고추의 생육(生育) 및 생산성(生産性)에 미치는 영향(影響))

  • Kim, Kwang-Yong;Park, Sang-Keun;Lim, Sang-Cheol
    • Korean Journal of Environmental Agriculture
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    • v.2 no.1
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    • pp.45-54
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    • 1983
  • This experiment was carried out to investigate effects of dense planting on the growth and productivity in varieties of hot pepper at open field. Three varieties (Joseng jinheung, Weonkyo No. 304 and Bulam house Putgochu) were sowed on Jan. 30th and transplanted on May 12th. The planting densities used were 3,300, 5,500 and 8,300 plants per 10a. The results obtained are as follows: 1) With higher plant density, the plant shape was changed from open-type to upright-type and the yield was increased, presumably due to the increase of leaf area index. 2) LAI and SLA per unit area were increased by denser planting. Wonkyo No. 304 presented the highest LAI and the lowest SLA. 3) Dry matter weight per unit area was also increased, however the LW/SW ratio was tended to decrease on denser planting. 4) Through crop growth analysis, it was determined that CGR and NAR were increased during the early growth stages and became decreased from last June. 5) Light intensity under the canopy formed by denser planting was decreased by 40% measured at 60 ㎝ above the soil surface. The vertical distribution of fruits became concentrated the upper part of plant. Fruit yield per unit area in denser planting was increased as compared to the conventional planting. The number of fruit setting decreased. 6) Generally, no differences in yield were detected in comparison between denser planting and conventional planting harvested on Aug. 30th and Oct. 15th, respectively. But Weonkyo No. 304 increased yield by 18% in denser planting.

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Derivation of the Synthetic Unit Hydrograph Based on the Watershed Characteristics (유역특성에 의한 합성단위도의 유도에 관한 연구)

  • 서승덕
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.17 no.1
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    • pp.3642-3654
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    • 1975
  • The purpose of this thesis is to derive a unit hydrograph which may be applied to the ungaged watershed area from the relations between directly measurable unitgraph properties such as peak discharge(qp), time to peak discharge (Tp), and lag time (Lg) and watershed characteristics such as river length(L) from the given station to the upstream limits of the watershed area in km, river length from station to centroid of gravity of the watershed area in km (Lca), and main stream slope in meter per km (S). Other procedure based on routing a time-area diagram through catchment storage named Instantaneous Unit Hydrograph(IUH). Dimensionless unitgraph also analysed in brief. The basic data (1969 to 1973) used in these studies are 9 recording level gages and rating curves, 41 rain gages and pluviographs, and 40 observed unitgraphs through the 9 sub watersheds in Nak Oong River basin. The results summarized in these studies are as follows; 1. Time in hour from start of rise to peak rate (Tp) generally occured at the position of 0.3Tb (time base of hydrograph) with some indication of higher values for larger watershed. The base flow is comparelatively higher than the other small watershed area. 2. Te losses from rainfall were divided into initial loss and continuing loss. Initial loss may be defined as that portion of storm rainfall which is intercepted by vegetation, held in deppression storage or infiltrated at a high rate early in the storm and continuing loss is defined as the loss which continues at a constant rate throughout the duration of the storm after the initial loss has been satisfied. Tis continuing loss approximates the nearly constant rate of infiltration (${\Phi}$-index method). The loss rate from this analysis was estimated 50 Per cent to the rainfall excess approximately during the surface runoff occured. 3. Stream slope seems approximate, as is usual, to consider the mainstreamonly, not giving any specific consideration to tributary. It is desirable to develop a single measure of slope that is representative of the who1e stream. The mean slope of channel increment in 1 meter per 200 meters and 1 meter per 1400 meters were defined at Gazang and Jindong respectively. It is considered that the slopes are low slightly in the light of other river studies. Flood concentration rate might slightly be low in the Nak Dong river basin. 4. It found that the watershed lag (Lg, hrs) could be expressed by Lg=0.253 (L.Lca)0.4171 The product L.Lca is a measure of the size and shape of the watershed. For the logarithms, the correlation coefficient for Lg was 0.97 which defined that Lg is closely related with the watershed characteristics, L and Lca. 5. Expression for basin might be expected to take form containing theslope as {{{{ { L}_{g }=0.545 {( { L. { L}_{ca } } over { SQRT {s} } ) }^{0.346 } }}}} For the logarithms, the correlation coefficient for Lg was 0.97 which defined that Lg is closely related with the basin characteristics too. It should be needed to take care of analysis which relating to the mean slopes 6. Peak discharge per unit area of unitgraph for standard duration tr, ㎥/sec/$\textrm{km}^2$, was given by qp=10-0.52-0.0184Lg with a indication of lower values for watershed contrary to the higher lag time. For the logarithms, the correlation coefficient qp was 0.998 which defined high sign ificance. The peak discharge of the unitgraph for an area could therefore be expected to take the from Qp=qp. A(㎥/sec). 7. Using the unitgraph parameter Lg, the base length of the unitgraph, in days, was adopted as {{{{ {T}_{b } =0.73+2.073( { { L}_{g } } over {24 } )}}}} with high significant correlation coefficient, 0.92. The constant of the above equation are fixed by the procedure used to separate base flow from direct runoff. 8. The width W75 of the unitgraph at discharge equal to 75 per cent of the peak discharge, in hours and the width W50 at discharge equal to 50 Per cent of the peak discharge in hours, can be estimated from {{{{ { W}_{75 }= { 1.61} over { { q}_{b } ^{1.05 } } }}}} and {{{{ { W}_{50 }= { 2.5} over { { q}_{b } ^{1.05 } } }}}} respectively. This provides supplementary guide for sketching the unitgraph. 9. Above equations define the three factors necessary to construct the unitgraph for duration tr. For the duration tR, the lag is LgR=Lg+0.2(tR-tr) and this modified lag, LgRis used in qp and Tb It the tr happens to be equal to or close to tR, further assume qpR=qp. 10. Triangular hydrograph is a dimensionless unitgraph prepared from the 40 unitgraphs. The equation is shown as {{{{ { q}_{p } = { K.A.Q} over { { T}_{p } } }}}} or {{{{ { q}_{p } = { 0.21A.Q} over { { T}_{p } } }}}} The constant 0.21 is defined to Nak Dong River basin. 11. The base length of the time-area diagram for the IUH routing is {{{{C=0.9 {( { L. { L}_{ca } } over { SQRT { s} } ) }^{1/3 } }}}}. Correlation coefficient for C was 0.983 which defined a high significance. The base length of the T-AD was set to equal the time from the midpoint of rain fall excess to the point of contraflexure. The constant K, derived in this studies is K=8.32+0.0213 {{{{ { L} over { SQRT { s} } }}}} with correlation coefficient, 0.964. 12. In the light of the results analysed in these studies, average errors in the peak discharge of the Synthetic unitgraph, Triangular unitgraph, and IUH were estimated as 2.2, 7.7 and 6.4 per cent respectively to the peak of observed average unitgraph. Each ordinate of the Synthetic unitgraph was approached closely to the observed one.

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