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Effect of Hydrosalpingeal Fluid on the Implantation in-vitro in a Murine Model (생쥐 배아의 체외배양 시 착상과정에 대한 난관수종액의 영향)

  • Jun, Jin-Hyun;Koong, Mi-Kyoung;Lim, Chun-Kyu;Kim, Soo-Kyung;Kang, Inn-Soo
    • Clinical and Experimental Reproductive Medicine
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    • v.27 no.2
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    • pp.159-164
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    • 2000
  • 연구목적:인간의 체외수정 및 배아이식술에서 난관수종을 갖는 환자에서 임신율과 착상률이 감소된다는 보고들이 있지만 이에 대한 명확한 기작은 밝혀지지 않았다. 본 연구에서는 생쥐 배아를 이용한 체외 착상모델에서 인간의 난관수종액(HSF)이 착상과정에 미치는 영향을 알아보고자 하였다. 연구재료 및 방법 난관수종액은 난관수종으로 수술을 받은 8명의 환자로부터 채취하였으며, 실험에 사용하기 전까지 냉동고에 보관하였다. 생쥐의 포배기 배아는 2-세포기배아를 3일 동안 배양하여 그 중 상태가 양호한 포배기 배아만을 선별하여 투명대를 제거한 후 사용하였다. 기본 배양액으로는 Ham's F-10을 사용하였으며, 배양 시 기본 배양액만을 사용한 경우를 group Ⅰ으로 하였고, 기본 배양액에 0.5% FBS를 첨가한 경우를 group Ⅱ, 0.5% FBS와 50% HSF를 첨가한 경우를 group Ⅲ , 100% HSF에 0.5% FBS를 첨가한 경우를 group Ⅳ,100% HSF만을 사용한 경우를 group Ⅴ로 하였다. 투명대를 제거한 포배기 배아를 각각의 HSF에 대한 5종류의 배양액에서 48시간 동안 배양하였다. 체외 착상 유무는 부착 부위에서 크기가 커진 영양세포들을 관찰하여 판정하였으며, 착상 부위의 표면적은 화상분석기를 이용하여 산출하였다. 결 과: 생쥐 배아의 체외 착상률은 group Ⅰ, Ⅱ, Ⅲ, Ⅳ, Ⅴ에서 각각 0%, 98.9%, 77.5%, 40.4%, 10.0%로 나타났으며, 착상 부위의 평균 표면적은 group Ⅱ, Ⅲ, Ⅳ, Ⅴ에서 각각 $74,675{\pm}25,201{\mu}m^2$, $59,024{\pm}25,877{\mu}m^2$, $45,156{\pm}22,654{\mu}m^2$, $38,254{\pm}17,115{\mu}m^2$이었다. 체외 착상률과 부위의 표면적은 HSF의 농도가 증가함에 따라 통계적으로 유의하게 감소하였다(p<0.001). 결론:인간의 난관수종액(HSF)은 생쥐 배아의 체외 착상과 영양배엽세포의 증식을 억제하는 것으로 확인되었으며, 이러한 원인이 난관수종을 갖는 환자에서 임신율이 낮은 것과 밀접한 관련이 있을 것으로 생각된다.

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Comparison of the Effects of Different Foot Positions During Body-lifting in Wheelchair on Shoulder Muscle Activities, Peak Plantar Pressure, Knee Flexion Angle, and Rating Perceived Exertion in Individuals With Spinal Cord Injury (휠체어에서 엉덩이 들기 동작 동안 발위치가 척수손상환자의 어깨 근활성도, 최대 족저압, 무릎굽힘 각도, 운동자각도에 미치는 효과 비교)

  • Lee, Wang-jae;Lim, One-bin;Yoon, Byoung-gu;Lee, Bum-suk;Yi, Chung-hwi
    • Physical Therapy Korea
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    • v.24 no.2
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    • pp.1-8
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    • 2017
  • Background: Individuals with spinal cord injury (SCI) rely on their upper limbs for body-lifting activity (BLA). While studies have examined the electromyography (EMG) and kinematics of the shoulder joints during BLA, no studies have considered foot position during BLA. Objects: This study compared the effects of different foot positions during BLA on the shoulder muscle activities, peak plantar pressure, knee flexion angle, and rating perceived exertion in individuals with SCI. Methods: The study enrolled 13 mens with motor-complete paraplegic SCI, ASIA (American Spinal Injury Association) A or B. All subjects performed BLA with the feet positioned on the wheelchair footrest and on the floor independently. Surface EMG was used to collect data from the latissimus dorsi, pectoralis major, serratus anterior, and triceps brachii. The peak plantar pressure was measured using pedar-X and the knee flexion angle with Image J. Borg's rating perceived exertion scale was used to measure the physical activity intensity level. The paired t-test was used to compare the shoulder muscle activities, peak plantar pressure, knee flexion angle, and rating perceived exertion between the two feet positions during BLA. Results: The activity of the latissimus dorsi, pectoralis major, serratus anterior, and triceps brachii and rating perceived exertion decreased significantly and the peak plantar pressure and knee flexion angle increased significantly when performing BLA with the feet positioned on the wheelchair footrest compared with on the floor (p<.05). Conclusion: These findings suggest that individuals with SCI may perform BLA with the feet positioned on the wheelchair footrest for weight-relief lifting to decrease the shoulder muscle activities and the rating perceived exertion and to increase the peak plantar pressure and the knee flexion angle.

A Study on the Estimation of Calcaneal Width Using a Correlation of Calcaneal Length and Width (종골 길이와 너비의 상관관계를 이용한 종골 너비 추정에 관한 연구)

  • Chun, Dong-il;Hwang, Shu Chiang;Cho, Jae-ho;Choi, Sung-Woo;Kim, Yong Beom;Won, Sung Hun
    • Journal of Korean Foot and Ankle Society
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    • v.21 no.2
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    • pp.61-65
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    • 2017
  • Purpose: The purpose of this study was to determine the correlation and ratio between the calcaneal length and width for predicting the width of calcaneus. Materials and Methods: A total of 190 feet (190 patients) were included based on computed tomography scans. The length of calcaneus (CL) was measured on the line connecting the center of a circle tangent to the cortical margin in the anterior and posterior parts of the calcaneus in a sagittal plane (W1, W2). The width of the calcaneus was defined as the horizontal line of each part (W1, W2, W3) on the same axial plane. The relationship between the measurement was determined through a correlation analysis. The reliability was assessed based on intraclass correlation coefficients. Results: The CL and widths of calcaneus (W1, W2, W3) had a good positive correlation (r=0.848 [W1/CL], r=0.738 [W2/CL], r=0.769 [W3/CL]; p<0.001). The mean CL and widths ratios were 0.33 (W1/CL), 0.37 (W2/CL), and 0.37 (W3/CL). Using these ratios to estimate the widths by multiplying each ratio by the measured calcaneal length, we found a difference between the estimated calcaneal widths and the actual measured calcaneal widths values was 0.25 mm, 0.43 mm, and 0.16 mm. All measurements showed good-to-excellent inter- and intraobserver reliability. Conclusion: This study analyzed the correlation and ratio between the length and width of the calcaneus. The results will help orthopedic surgeons fixate screws in a stable manner to prevent iatrogenic injuries to the medial neurovascular structures of the calcaneus.

Development of Biotope Evaluation Indexes for Improving Bird Diversity (조류 다양성 확보를 위한 비오톱 평가지표개발)

  • Chae, Jin-Hwak;Koo, Tae-Hoe
    • Journal of Environmental Policy
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    • v.4 no.1
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    • pp.113-127
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    • 2005
  • This study was aimed at developing an application method of biotope evaluation indexes to secure bird diversity on urban biotope that has high intensity of land use. The main purpose of the study was to plan the effective restoration method of sound urban biotope from the ecological aspects after evaluating the relationship between the biotope evaluation indexes. The biotope area, number of insect species, distance from adjacent road, biotope area index were surveyed on each designated site in order to analyze the evaluation indexes that influenced bird diversity in urban biotope. The analysis of the relationship between biotope evaluation. indexes showed the following results number of bird species and number of insect species > number of bird species and biotope area index > number of insect species and biotope area index, that is, the number of insect species and biotope area index had a specific effect on the bird diversity. The equation derived from the above result is 'number of bird species = 6.124 $\times$ biotope area index + 0.095 $\times$ number. of insect species - 1.197' ($R^2=0.716$, F=27.743, P<0.001). Therefore, even a small area can be helpful for promoting bird diversity in the urban area if the biotope area index and diversity of vertical landscape for insects are maintained.

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Effects of Soil Addition and Subsoil Plowing on the Change of Soil Chemical Properties and the Reduction of Root-Knot Nematode in Continuous Cropping Field of Oriental Melon (Cucumis melo L.) (시설참외 연작재배지 토양의 객토 및 심토반전이 토양 화학성 변화와 토양선층 억제효과에 미치는 영향)

  • Jun, Han-Sik;Park, Woo-Chul;Jung, Jae-Sik
    • Korean Journal of Environmental Agriculture
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    • v.21 no.1
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    • pp.1-6
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    • 2002
  • To elucidate the effect of soil addition and subsoil plowing on the change of sell chemical properties and the reduction of root-knot nematode, this experiment carried out in continuous cropping field of protected oriental melon (Cucumis melo L.). Soil addition reduced electric conductivity (E. C.) from 4.3 to 1.8 dS/m (58%), available $P_2O_5$ from 406 to 182 mg/kg (55%) and organic matter content from 16 to 11 g/kg (31%). Population densities of root-knot nematode in soil reduced as much as 89%, 84%, and 69% at first year, third years, and of five years later, respectively The effects of subsoil plowing were similar to that of soil addition. E. C. and phosphate were reduced from 4.30 to 1.98 dS/m (54%) and phosphate from 406 to 329 mg/kg (19%), respectively. Population densities of root-knot nematode reduced as much as 71%, 67%, and 42% after 1, 3, and 5 years, respectively Subsoil plowing reduced nematode densities only for three years.

A Study on the Factors Affecting the Recognition of Self-determination Right and the Necessity of Decision Support for the Elderly Living Alone (독거노인의 자기결정권 인식과 의사결정 지원 필요성에 대한 영향요인 연구)

  • Kim, Hyojung
    • The Journal of the Korea Contents Association
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    • v.19 no.6
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    • pp.125-134
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    • 2019
  • The purpose of this study is to explore the direction of decision support for senior citizens living alone by identifying the level of self-determination and desire for decision support. The survey was conducted on 300 elderly people living alone in five districts of Seoul. The analysis results are as follows: First, it has been shown that elderly living alone have thought that it is most important to reflect their decisions when deciding where to live. Second, respondents highly recognized that the need for support decision making as the age group grew. Third, it was found that the factors affecting the perception of the need to support decision-making by senior citizens living alone were age, trust in family and recognition of importance in decision-making. The study suggested the need for a specific, institutionalized system to support decision making for elderly living alone.

Self-poisoning as a Target Group for Prevention of Suicide (자살 예방을 위한 집중관리 대상으로서의 중독)

  • Kwak, Moon Hwan;Kang, Hyun Young;Lee, Si Jin;Han, Kap Su;Kim, Su Jin;Lee, Eu Jung;Lee, Sung Woo
    • Journal of The Korean Society of Clinical Toxicology
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    • v.16 no.2
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    • pp.93-101
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    • 2018
  • Purpose: The Korean government has tried to decrease the suicide death rate over the last decade. Suicide attempts, particularly non-fatal attempts, are the most powerful known risk factor for a completed suicide. An analysis of suicide attempt methods will help establish the effective preventive action of suicide. Fit prevention according to the method of suicide attempt may decrease the incidence of suicide death. Self-poisoning is suggested as a major method of both suicide attempts and suicide death. The aim of this study was to determine if a self-poisoning patient is a suitable target for the prevention of the suicide. Methods: This was retrospective analysis of a prospective cohort, which included patients who presented to the emergency department (ED) after a self-harm or suicide attempt from Jan 2013 to Dec 2017. The proportion of methods in suicide attempts, psychological consultation, and fatality according to the suicide attempt method were analyzed. The types of poison were also analyzed. Results: Poisoning was the most common method of suicide attempts (52.1%). The rate of psychological consultations were 18.8% for all patients and 29.1% for poison patients (p<0.001). The rate of mortality in poisoning was 0.6%. Psychological consultation was performed more frequently in admission cases than discharged cases. The most common materials of poisons was psychological medicines and sedatives that had been prescribed at clinics or hospital. Conclusion: Self-poisoning is a major method of suicide attempt with a high rate of psychiatric consultation, low mortality rate, versus others methods. The prevention of suicide death for suicide attempts may focus on self-poisoning, which is the major method of suicide attempts. A suitable aftercare program for self-poisoning may be an effective method for preventing suicide if an early diagnosis and management of psychiatric disorders through psychiatric consultation can be made, and early connection to social prevention program for non-fatal patients are possible.

The Relationship Between Korean Handwriting Skill and Visual Fixation (비장애 아동의 한글쓰기 숙련도와 시선고정 간의 관련성)

  • Hong, Mi Young;Lee, Cho Hee;Kim, Eunbin;Lee, Onseok;Kim, Eun Young
    • The Journal of Korean Academy of Sensory Integration
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    • v.17 no.1
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    • pp.1-8
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    • 2019
  • Objective : This paper aimed to measure the relationship between the performance of Korean handwriting and visual fixation for children. Methods : Twenty-one typically developing children aged 7 to 9 years participated in the study. The children performed Korean handwriting task wearing Tobii Pro Glasses 2. The Korean handwriting task consisted of 10 words from elementary school textbooks. The handwriting skill was measured by the coefficient variation of the letter size and the fixation cound and duration. Correlation analysis was performed to investigate the relation between visual fixation and the coefficient variation of the letter size. Results : The results showed that the visual fixation per second was positively correlated with Korean handwriting vertical size coefficient variation, indicating that the more consistent the vertical size of the letter, the smaller the fixation count per second. Conclusion : The results suggested a relation between the performance of Korean handwriting and visual fixation in typically developing children. This study is the first attempt to measure eye movement during the Korean handwriting process, and suggests a future direction for research on students' development in writing Korean.

Study on the Anti-stress Effect of Chenwangbosim-Dan Extract∘Fermented Rice Extract (GABA ː gamma-aminobutyric acid 30%) Complex : A double-blind, randomized, placebo-controlled study (천왕보심단(天王補心丹)∘쌀발효추출물(GABAːgamma-aminobutyric acid 30%) 복합물의 항(抗)스트레스에 대한 유효성 연구ː무작위 배정 및 이중맹검)

  • Choyoung, Hong;Seo, Sang Gwon;Seunggon, Na;Hosong, Cho;Mi-Kyung, Lee;Mu Hyun, Jin;Seok-Seon, Roh;Youngsung, Ju
    • The Korea Journal of Herbology
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    • v.38 no.2
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    • pp.1-7
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    • 2023
  • Objectives : From this study, we sight to identify Anti-stress effect of Chenwangbosim-Dan (Tianwangbuxin-Dan) Extract∘Fermented Rice Extract (GABA : gamma-aminobutyric acid 30%) Complex Methods : In order to measure the degree of functional evaluation of tension relief due to stress, it was evaluated whether the psychological indicators VAS(Visual Analog Scale) and POMS(Profile Of Mood State) were improved. Which is generally used for stress measurement and has a high frequency of clinical use. All clinical trial subjects were required to take the drug once a day at 9 ± 30 min in the morning for 2 weeks. Results : After intake, the group that Chenwangbosim-Dan(Tianwangbuxin-Dan) Extract∘Fermented Rice Extract (GABA : gamma-aminobutyric acid 30%) Complex had a statistically significant improvement effect compared to before intake due to an improved 30.81% of the VAS scores. The POMS-T score improved 54.13%, the POMS-V score improved 117.5% and the POMS-F score improved 59%, which had a statistically significant effect compared to Placebo group (p<0.001). There is a significant difference between two group, so even considering the placebo effect, the anti stress effect was confirmed in the Chenwangbosim-Dan (Tianwangbuxin-Dan) Extract∘Fermented Rice Extract (GABA : gamma-aminobutyric acid 30%) Complex intake group. Conclusions : The above results showed that the Chenwangbosim-Dan(Tianwangbuxin-Dan) Extract∘Fermented Rice Extract (GABA : gamma-aminobutyric acid 30%) Complex had an anti-stress effect. Therefore, it is expected to be developed as a safe and comfortable mental care health functional food that can reduce the risk of drug abuse.

Analytical Study of Geometric Nonlinear Behavior of Cable-stayed Bridges (사장교의 기하학적 비선형 거동의 해석적 연구)

  • Kim, Seungjun;Lee, Kee Sei;Kim, Kyung Sik;Kang, Young Jong
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.30 no.1A
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    • pp.1-13
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    • 2010
  • This paper presents an investigation on the geometric nonlinear behavior of cable-stayed bridges using geometric nonlinear finite element analysis method. The girder and mast in cable-stayed bridges show the combined axial load and bending moment interaction due to horizontal and vertical forces of inclined cable. So these members are considered as beam-column member. In this study, the nonlinear finite element analysis method is used to resolve the geometric nonlinear behavior of cable-stayed bridges in consideration of beam-column effect, large displacement effect (known as P-${\delta}$ effect) and cable sag effect. To analyze a cable-stayed bridge model, nonlinear 6-degree of freedom frame element and nonlinear 3-degree of freedom equivalent truss element is used. To resolve the geometric nonlinear behavior for various live load cases, the initial shape analysis is performed for considering dead load before live load analysis. Then the geometric nonlinear analysis for each live load case is performed. The deformed shapes of each model, load-displacement curves of each point and load-tensile force curves for each cable are presented for quantitative study of geometric nonlinear behavior of cable-stayed bridges.