• The Korean Journal of Cytopathology
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• v.7 no.1
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• pp.38-43
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• 1996

Epstein-Barr virus(EBV) is associated with a wide spectrum of benign and malignant disorders including leukoplakia, Hodgkln's lymphoma, central nervous system lymphoma, peripheral T cell lymphoma and nasopharyngeal undifferentiated carcinoma. There are several distinctive aspects of biology of the virus that are important in investigation of virus in clinical specimens. The abundant expression of the EBER mRNA transcripts makes possible the sensitive detection of latent expression in EBV-associated tumors. Although there has been a dramatic increased interest in the direct characterization of EBV in clinical specimens, there have been few studios about the effective and reliable positive controls in performing in situ hybridization technique for EBV, especially on paraffin-em bedded tissue. We applied Burkitts lymphoma ceil line as positive control in EBV in situ hydridization using Oncor Kit. The cell block of Burkitt lymphoma cell line(CCL85 EB-3) showed strong and specific positivity for EBER in situ in nuclei of EBV infected cells.

• Tunnel and Underground Space
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• v.7 no.4
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• pp.323-333
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• 1997

A rock mass is usually classified by the results of geological survey and laboratory tests on rock specimens in order to obtain the adequate properties for the numerical analysis. For these purposes a rock mass strength is estimated based on the empirical criterion proposed by Hoek and Brown and a modulus of deformation is taken with the empirical relations developed by Bieniawski, Serafim and Pereira. In addition, the $K_o$ value which is the ratio of the horizontal stress to the vertical stress is one of the most important input data in the numerical analysis. Its role on a tunnel stability analysis could be verified with the numerical results taken by a finite difference code or a distinct element code. However, a deduced value used to be applied for the $K_o$ value in most of tunnel designs, even though the patterns of stress tensor are variable with regions and depths. Thus in situ stresses were measured by a hydraulic fracturing technique on several tunnel sites and applied directly to the tunnel design for the enhancement of its precision. With those informations on in situ stresses, the safe design should be obtained economically on the road or subway tunnels.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.5 no.6
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• pp.1128-1135
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• 2001

In this paper, a new HSG-Si formation technology, "seeding method', which employs Si$_2$H$_{6}$-molecule irradiation and annealing, was applied for realizing 64Mbit DRAMs. By using this technique, grain size controlled HSG-Si can be fabricated on in-situ phosphorous-doped amorphous-Si electrode. The new HSG-Si fabrication technology achieves twice the storage capacitance with high reliability for the stacked capacitors. In this technique, optimum process conditions of the phosphorous concentration, storage polysilicon deposition temperature and thickness of hemispherical grain silicon are in the range of 3.0-4.0E19atoms/㎤, 53$0^{\circ}C$ and 400$\AA$, respectively. In the 64M bit DRAM capacitor using optimum process conditions, limit thickness of dielectric nitride is about 65$\AA$.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.7
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• pp.73-83
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• 2017

This study was conducted to evaluate the chemical composition, digestibility, and energy value of 15 non-conventional feeds produced in South Korea as ruminant feeds. Three Hanwoo steers (body weight, $520{\pm}20.20kg$) fitted with a permanent rumen cannula and duodenal cannula were housed individually in tie-stall barns, followed by a 14-day adaptation period and 3-day experimental period. Chemical composition analysis, in situ nylon bag, and mobile bag technique were used as experiments. As a result of the chemical composition analysis offeeds, crude protein (CP) contentsofmalt meal, perilla meal, soy sauce cake, and soymilk residue were greater than 30%. As a result of the degradability characteristics analysis of feeds using an in situ nylon bag, rumen undegraded protein (RUP) contents of beet pulp, brewer's grain, coffee meal, malt meal, milo bran, perilla meal, ramen residue, and soymilk residue were greater than 50%. Analysis of total digestible nutrient (TDN) values of feeds using an in situ mobile bag showed that TDN values of beet pulp, brewer's grain, makgeolli residue, milo bran, perilla meal, ramen residue, rice bran, soy sauce cake, soybean curd cake, soymilk residue, and wheat bran weregreater than 50%. In summary, these non-conventional feeds have high potential value as good feed resources to replace formulated feeds or roughage. Therefore, the chemical composition, digestibility, and energy value of non-conventional feeds obtained from this study can be used as base data for the manufacture of ruminant total mixed ration (TMR) with improved feed efficiency, reduced feed costs, and reduction of environmental pollution.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.10
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• pp.6131-6134
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• 2013

Background: The two basic methods that are currently accepted to identify the HER2 status are immunohistochemistry and flyorescence in situ hybridization (FISH). The aim of this study was to perform the dual-color silver in situ hybridization (dc-SISH) technique as an alternative to FISH. Materials and Methods: A total of 40 invasive breast carcinoma cases were assessed for HER2 gene amplification by FISH and dual-color SISH. Results: Significant correlation was found in the HER2 expression results obtained with the two approaches (p=0.001, p<0.05). The concordance rate was 92.3%. Conclusions: Foutine practical use of the dc-SISH method, which is much easier to apply, score, and evaluate, has many advantages. HER2 and CEN17 status can be evaluated simultaneously with the newly developed "Dual-Color Probe". All these specifications and the reliable results obtained support the widespread use of SISH technique in clinical practice.

• Journal of the Korean Applied Science and Technology
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• v.18 no.4
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• pp.273-284
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• 2001

A series of microcapsule were synthesized by using several PCM(Phase Change Material) as a core material and gelatin/arabic gum, melamine/formaldehyde as a shell material. Coacervation technique and in situ polymerization were adopted in synthesizing microcapsules. In the microencapsulation by coacervation, tetradecane and octadecane were used as core materials. In the microencapsulation by situ polymerization tetradecane, pentadecane, hexadecane, heptadecane, octadecane, and nonadecane were used as core material. The synthesized microcapsule was examined to observe the shape of the microcapsule. The particle size analysis was performed by particle size analyzer. The thermal properties(e.g. melting point, heat of melting, crystallization temperature, heat of crystallization, differences between melting point and crystallization temperature) were obtained by DSC(Differential Scanning Calorimeter). The stirring rate effect was investigated during the microencapsulation. It was found that with increasing the stirring rate much smaller microcapule was produced. However, this did not necessarily lead to formation of spherical microcapsule.

• Journal of the Korean Society for Precision Engineering
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• v.21 no.10
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• pp.102-108
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• 2004

In this paper, we studied a vibration problem that is critical and common to most precision measurement systems. For micro mechanical part measurements, results obtained from the vision-based precision measurement system may contain errors due to the vibration. In order to defeat this generic problem, for the current study, a PC based image processing technique was used first, to assess the effect of the vibration to the precision measurement and second, to develop an in-situ calibration algorithm that automatically compensate the measurement results in real time. We used a set of stereoscopic CCD cameras to acquire the images for the dimensional measurement and the reference measurement. The mapping function was obtained through the in-situ calibration to compensate the measurement results and the statistical analysis for the actual results is provided in the paper. Based on the current statistical study, it is expected to obtain high precision results for the micro measurement systems.

• Proceedings of the Korean Geotechical Society Conference
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• 1999.03a
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• pp.439-446
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• 1999

In situ experimental studies were Performed at Yongjong Island Geotechnical Experimental Site to evaluate the ground densification on sand deposited. Standard penetration test, cone penetration test, and SASW test were performed and soil profiles and quality of ground improvements were evaluated. The feasibility of applying SASW method were verified by comparing test results. The evaluation technique of in-situ density using SASW and resonant column tests was proposed, and the reliability of proposed method was verified by performing case studies.

• Journal of Korean Society for Atmospheric Environment
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• v.17 no.E4
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• pp.169-181
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• 2001

To test the compatibility of differential optical absorption spectroscopy (DOAS) and conventional in-situ monitoring technique we conducted a comparative analysis of the two systems using hourly measurement data sets of three criteria pollutants including No$_{2}$O$_{3}$, and SO$_{2}$ collected in months between April and June of 2001 at Sung Man city, Kyung Gi Province, Korea. The results of our comparative analysis were useful to evaluate the various aspects of DOAS performance, of particular the level of agreement with the counterpart method through computation of percent differences and correlation analysis. Interpretation of the mixing ratio data for those chemical species was however confined in terms of explaining the differences affected by the changes in environmental conditions because measurements of important meteorological parameters were limited during most of the study period. Nevertheless, the overall results of this study strongly demonstrated that the mixing ratio of major pollutants measured by the two different systems maintain strong compatibility from various respects.

• Corrosion Science and Technology
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• v.2 no.6
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• pp.277-282
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• 2003

Holographic interferometry, an electromagnetic method, was used to study corrosion of carbon steel, aluminum and copper nickel alloys in NaOH, KCI and $H_2SO_4$ solutions respectively. The technique, called electrochemical emission spectroscopy, consisted of in-situ monitoring of changes in the number of fringe evolutions during the corrosion process. It allowed a detailed picture of anodic dissolution rate changes of alloys. The results were compared to common corrosion measurement methods such as linear polarization resistance measurements and electrochemical impedance spectroscopy. A good agreement between both data was found, thus indicating that holographic interferometry can be a very powerful technique for in-situ corrosion monitoring.