• Title/Summary/Keyword: In-Situ Observation

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Captive breeding of endangered betta fish, Betta rubra, under laboratory conditions

  • Agus Priyadi;Asep Permana;Eni Kusrini;Erma Primanita Hayuningtyas;Bastiar Nur;Lukman;Josie South;Sawung Cindelaras;Sulasy Rohmy;Rendy Ginanjar;Muhamad Yamin;Djamhuriyah S Said;Tutik Kadarini;Darmawan Setia Budi
    • Fisheries and Aquatic Sciences
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    • v.27 no.4
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    • pp.213-224
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    • 2024
  • Betta rubra, classified as endangered fish species by the International Union for Conservation of Nature (IUCN), has been successfully bred and raised in captivity for two generations under laboratory conditions. This study aimed to provide comprehensive information on the captive breeding of B. rubra, focusing on various parameters crucial for ex-situ conservation and domestication. The research involved breeding trials, embryo and larvae observation, first feeding experiments, larva and fry rearing trials, and the evaluation of growth and reproduction in two generations. The study revealed that the female B. rubra, with an average total length of 5.17 ± 0.15 cm and weight of 1.61 ± 0.06 g, produced an average of 73.67 ± 7.09 eggs, 34.33 ± 5.13 total larvae, and exhibited a hatching rate of 46.67 ± 5.77%. The embryogenesis process commenced on the day of spawning (dps) and continued until the eggs hatched at 6 dps. Larvae development and yolk absorption occurred from 0 to 6 days post-hatching (dph). The study also examined the impact of different initial feeding options, with chopped Tubifex resulting in the most significant in- crease (p < 0.05) in length. The growth pattern of B. rubra larvae showed slow initial growth during the first seven days, followed by a rapid exponential growth phase from day 8 to day 39. Two generations of B. rubra (G1 and G2) were successfully bred in captivity, with G2 showing a better tendency for growth in length and weight compared to G1. Notably, there were no significant differences (p > 0.05) in reproductive success between the wild-origin broodstock (G0), G1, or G2. This research contributes valuable insights into the captive breeding of B. rubra and its early life stages, offering critical information for the conservation and sustainable management of this endangered species. Further research is needed to explore the long-term effects of domestication on behavior, physiology, and phenotypic diversity.

In situ posture of anterior body of Metagonimus yokogawai in experimentally infected dog (개의 실험적 요꼬가와흡충증에서 충체의 자세)

  • 장영기;강신영
    • Parasites, Hosts and Diseases
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    • v.23 no.2
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    • pp.203-213
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    • 1985
  • The in situ posture of anterior body of Metagonimus yokogawai was observed in experimental metagonimiasis of dog. The metacercariae were collected from naturally infected sweetfish by peptic digestion; a total of 8 dogs was orally infected with 10,000 metacercariae respectively. Two dogs were killed on 3 days, 9 days, 4 weeks and 10 weeks after the infection. The postures of worms in histological section of small intestine and of whole worms collected from the fixed intestinal mucosa were examined by light and scanning electron microscope. The results were summarized as follows: 1. The recovery rates of worms were 42.6% on 3 days, 55.0% on 9 days, 33.2% on 4 weeks and 9. 8% on 10 weeks after the infection respectively. 2. In histological sections of small intestine, most of worms were found at intervillous spaces as ovoid sections of posterior body. However, many worms, especially in 3 day-old worms, revealed protruded anterior body in glandular lumens of crypt. Some sections of anterior body were bifurcated or sacculated. 3. The worms collected from fixed intestinal mucosa under dissecting microscope exhibited a variety of postures. Many worms showed flat shapes with a concavity or curvatures. However, in many worms, the anterior body made a single or multiple protrusions. 4. By SEM observation of protruded anterior body, a longitudinal groove was found. 5. The frequency of worms with protruded anterior body decreased in 9 days, 4 weeks and 10 weeks than in 3 day-old worms. The above findings indicated that the anterior body of juvenile and adult M. yokogawai protruded to lumens of glandular crypt by folding their lateral portions to make a reversible tubelike structure. Frequent multiple protrusions were considered to be made to adapt the microniche of glandular crypts of dog intestine.

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Validation of Satellite Scatterometer Sea-Surface Wind Vectors (MetOp-A/B ASCAT) in the Korean Coastal Region (한반도 연안해역에서 인공위성 산란계(MetOp-A/B ASCAT) 해상풍 검증)

  • Kwak, Byeong-Dae;Park, Kyung-Ae;Woo, Hye-Jin;Kim, Hee-Young;Hong, Sung-Eun;Sohn, Eun-Ha
    • Journal of the Korean earth science society
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    • v.42 no.5
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    • pp.536-555
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    • 2021
  • Sea-surface wind is an important variable in ocean-atmosphere interactions, leading to the changes in ocean surface currents and circulation, mixed layers, and heat flux. With the development of satellite technology, sea-surface winds data retrieved from scatterometer observation data have been used for various purposes. In a complex marine environment such as the Korean Peninsula coast, scatterometer-observed sea-surface wind is an important factor for analyzing ocean and atmospheric phenomena. Therefore, the validation results of wind accuracy can be used for diverse applications. In this study, the sea-surface winds derived from ASCAT (Advanced SCATterometer) mounted on MetOp-A/B (METeorological Operational Satellite-A/B) were validated compared to in-situ wind measurements at 16 marine buoy stations around the Korean Peninsula from January to December 2020. The buoy winds measured at a height of 4-5 m from the sea surface were converted to 10-m neutral winds using the LKB (Liu-Katsaros-Businger) model. The matchup procedure produced 5,544 and 10,051 collocation points for MetOp-A and MetOp-B, respectively. The root mean square errors (RMSE) were 1.36 and 1.28 m s-1, and bias errors amounted to 0.44 and 0.65 m s-1 for MetOp-A and MetOp-B, respectively. The wind directions of both scatterometers exhibited negative biases of -8.03° and -6.97° and RMSE values of 32.46° and 36.06° for MetOp-A and MetOp-B, respectively. These errors were likely associated with the stratification and dynamics of the marine-atmospheric boundary layer. In the seas around the Korean Peninsula, the sea-surface winds of the ASCAT tended to be more overestimated than the in-situ wind speeds, particularly at weak wind speeds. In addition, the closer the distance from the coast, the more the amplification of error. The present results could contribute to the development of a prediction model as improved input data and the understanding of air-sea interaction and impact of typhoons in the coastal regions around the Korean Peninsula.

CHARACTERISTICS OF HETEROEPITAXIALLY GROWN $Y_2$O$_3$ FILMS BY r-ICB FOR VLSI

  • Choi, S.C.;Cho, M.H.;Whangbo, S.W.;Kim, M.S.;Whang, C.N.;Kang, S.B.;Lee, S.I.;Lee, M.Y.
    • Journal of the Korean institute of surface engineering
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    • v.29 no.6
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    • pp.809-815
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    • 1996
  • $Y_2O_3$-based metal-insulator-semiconductor (MIS) structure on p-Si(100) has been studied. Films were prepared by UHV reactive ionized cluster beam deposition (r-ICBD) system. The base pressure of the system was about $1 \times 10^{-9}$ -9/ Torr and the process pressure $2 \times 10^{-5}$ Torr in oxygen ambience. Glancing X-ray diffraction(GXRD) and in-situ reflection high energy electron diffracton(RHEED) analyses were performed to investigate the crystallinity of the films. The results show phase change from amorphous state to crystalline one with increasingqr acceleration voltage and substrate temperature. It is also found that the phase transformation from $Y_2O_3$(111)//Si(100) to $Y_2O_3$(110)//Si(100) in growing directions takes place between $500^{\circ}C$ and $700^{\circ}C$. Especially as acceleration voltage is increased, preferentially oriented crystallinity was increased. Finally under the condition of above substrate temperature $700^{\circ}C$ and acceleration voltage 5kV, the $Y_2O_3$films are found to be grown epitaxially in direction of $Y_2O_3$(1l0)//Si(100) by observation of transmission electron microscope(TEM). Capacitance-voltage and current-voltage measurements were conducted to characterize Al/$Y_2O_3$/Si MIS structure with varying acceleration voltage and substrate temperature. Deposited $Y_2O_3$ films of thickness of nearly 300$\AA$ show that the breakdown field increases to 7~8MV /cm at the same conditon of epitaxial growing. These results also coincide with XPS spectra which indicate better stoichiometric characteristic in the condition of better crystalline one. After oxidation the breakdown field increases to 13MV /cm because the MIS structure contains interface silicon oxide of about 30$\AA$. In this case the dielectric constant of only $Y_2O_3$ layer is found to be $\in$15.6. These results have demonstrated the potential of using yttrium oxide for future VLSI/ULSI gate insulator applications.

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Ground Subsidence Estimation in a Coastal Reclaimed Land Using JERS-1 L-band SAR Interferometry (JERS-1 L-band SAR Interferometry 를 이용한 연안매립지 지반침하 관측)

  • 김상완;이창욱;원중선
    • Economic and Environmental Geology
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    • v.35 no.5
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    • pp.465-478
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    • 2002
  • We measured subsidences occurred in a coastal reclaimed land, Noksan industrial complex, from May 2, 1996 to February 21, 1998, using 5 interferograms of JERS-1 L-band SAR. SAR with a spatial resolution of about 16 m can detect the two-dimensional distribution of subsidence that is difficult to be estimated from in situ measurements. Accuracy of the subsidences estimated by 2-pass DInSAR was evaluated using the measurements of Magnetic Probe Extensometer (accuracy of :${\pm}$1 mm) installed at 42 stations. DInSAR measurements were well correlated with the field measurements showing an average correlation coefficient of 0.77. The correlation coefficient was further improved to be 0.87 (with RMSE of 1.44 cm) when only highly coherenced (>0.5) pixels were used. The slope of regression line was 1.04, very close to the unit value. In short, DInSAR measurements have a good linear relation with field measurements so that we can effectively detect a subsidence in the coastal reclaimed area especially using pixels of high coherence (>0.5). The maximum accumulated subsidence was about 60 cm in the study area, while the subsidence in the northern and south western areas were less than 20 cm. The resuts show that DInSAR is extremely useful for geotechnical applications as well as observation of natural deformation.

A study of artificial neural network for in-situ air temperature mapping using satellite data in urban area (위성 정보를 활용한 도심 지역 기온자료 지도화를 위한 인공신경망 적용 연구)

  • Jeon, Hyunho;Jeong, Jaehwan;Cho, Seongkeun;Choi, Minha
    • Journal of Korea Water Resources Association
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    • v.55 no.11
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    • pp.855-863
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    • 2022
  • In this study, the Artificial Neural Network (ANN) was used to mapping air temperature in Seoul. MODerate resolution Imaging Spectroradiomter (MODIS) data was used as auxiliary data for mapping. For the ANN network topology optimizing, scatterplots and statistical analysis were conducted, and input-data was classified and combined that highly correlated data which surface temperature, Normalized Difference Vegetation Index (NDVI), Enhanced Vegetation Index (EVI), time (satellite observation time, Day of year), location (latitude, hardness), and data quality (cloudness). When machine learning was conducted only with data with a high correlation with air temperature, the average values of correlation coefficient (r) and Root Mean Squared Error (RMSE) were 0.967 and 2.708℃. In addition, the performance improved as other data were added, and when all data were utilized the average values of r and RMSE were 0.9840 and 1.883℃, which showed the best performance. In the Seoul air temperature map by the ANN model, the air temperature was appropriately calculated for each pixels topographic characteristics, and it will be possible to analyze the air temperature distribution in city-level and national-level by expanding research areas and diversifying satellite data.

Change Detection of Damaged Area and Burn Severity due to Heat Damage from Gangwon Large Fire Area in 2019 (2019년 강원도 대형산불지역의 열해 피해로 인한 피해강도 변화 탐색)

  • Won, Myoungsoo;Jang, Keunchang;Yoon, Sukhee;Lee, HoonTaek
    • Korean Journal of Remote Sensing
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    • v.35 no.6_2
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    • pp.1083-1093
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    • 2019
  • The purpose of this study is to detect the burned area change by direct burning of tree canopies and post-fire mortality of trees via analyzing satellite imageries from the Korea multi-purpose satellite-2 and -3 (KOMPSAT-2 and -3) for two large-fires over the Goseong-Sokcho and Gangneung-Donghae regions in April 2019. For each case, the burned area was compared between two dates: the day when the fire occurred and 15-18 days after it. As the results, within these two dates, there was no substantial difference in burned area of sites whose severities were marked as "Extreme", but sites with "High" and "Low" severities showed significant differences in burned area between the two dates. These differences were resulted from the lagged post-fire browning of canopies which was detected by images from in-situ observation,satellite, and the unmanned aerial vehicle. The post-fire browning started after 3-4 days and became apparent after 10-15 days. This study offers information about the timing to quantify the burned area by large fire and about the mechanism of post-fire mortality. Also, the findings can support policy makers in planning the restoration of the damaged areas.

Development of Suspended Sediment Algorithm for Landsat TM/ETM+ in Coastal Sea Waters - A Case Study in Saemangeum Area - (Landsat TM/ETM+ 연안 부유퇴적물 알고리즘 개발 - 새만금 주변 해역을 중심으로 -)

  • Min Jee-Eun;Ahn Yu-Hwan;Lee Kyu-Sung;Ryu Joo-Hyung
    • Korean Journal of Remote Sensing
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    • v.22 no.2
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    • pp.87-99
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    • 2006
  • The Median Resolution Sensors (MRSs) for land observation such as Landsat-ETM+ and SPOT-HRV are more effective than Ocean Color Sensors (OCSs) for studying of detailed ecological and biogeochemical components of the coastal waters. In this study, we developed suspended sediment algorithm for Landsat TM/ETM+ by considering the spectral response curve of each band. To estimate suspended sediment concentration (SS) from satellite image data, there are two difference types of algorithms, that are derived for enhancing the accuracy of SS from Landsat imagery. Both empirical and remote sensing reflectance model (hereafter referred to as $R_{rs}$ model) are used here. This study tried to compare two algorithm, and verified using in situ SS data. It was found that the empirical SS algorithm using band 2 produced the best result. $R_{rs}$ model-based SS algorithm estimated higher values than empirical SS algorithm. In this study we used $R_{rs}$ model developed by Ahn (2000) focused on the Mediterranean coastal area. That's owing to the difference of oceanic characteristics between Mediterranean and Korean coastal area. In the future we will improve that $R_{rs}$ model for the Korean coastal area, then the result will be advanced.

Formation Behavior and Properties of PEO Films on AZ91 Mg Alloy in 0.1 M NaOH + 0.05 M NaF Solution Containing Various Na2SiO3 Concentrations (AZ91 마그네슘 합금의 플라즈마 전해산화 피막 형성 및 물성에 미치는 0.1 M NaOH + 0.05 M NaF 용액 중 Na2SiO3 농도의 영향)

  • Kwon, Duyoung;Song, Pung-Keun;Moon, Sungmo
    • Journal of the Korean institute of surface engineering
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    • v.53 no.2
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    • pp.59-66
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    • 2020
  • Effects of Na2SiO3 concentration added into 0.1 M NaOH + 0.05 M NaF solution on the formation behavior and properties of PEO films on AZ91 Mg alloy were investigated under 1200 Hz of alternating current (AC) by voltage-time curves, in-situ observation of arc generation behavior and measurements of film thickness, surface roughness and micro vickers hardness. In the absence of Na2SiO3 in the 0.1 M NaOH + 0.05 M NaF solution, about 4 ㎛ thick PEO film was formed within 1 min and then PEO film did not grow but white spots were formed by local burning. Addition of Na2SiO3 up to 0.2 M caused more increased formation voltage and growth of PEO film with uniform generation of arcs. Addition of Na2SiO3 from 0.2 M to 0.4 M showed nearly the same voltage-time behavior and uniform arc generation. Addition of Na2SiO3 more than 0.5 M resulted in a decrease of formation voltage and non-uniform arc generation due to local burning. PEO film growth rate increased with increasing added Na2SiO3 concentration but maximum PEO film thickness was limited by local burning if added Na2SiO3 concentration is higher than 0.5 M. Surface roughness of PEO film increased with increasing added Na2SiO3 concentration and appeared to be proportional to the PEO film thickness. PEO film hardness increased with increasing added Na2SiO3 concentration and reached a steady-state value of about 930 HV at more than 0.5 M of added Na2SiO3 concentration.

Establishment of Thermal Infrared Observation System on Ieodo Ocean Research Station for Time-series Sea Surface Temperature Extraction (시계열 해수면온도 산출을 위한 이어도 종합해양과학기지 열적외선 관측 시스템 구축)

  • KANG, KI-MOOK;KIM, DUK-JIN;HWANG, JI-HWAN;CHOI, CHANGHYUN;NAM, SUNGHYUN;KIM, SEONGJUNG;CHO, YANG-KI;BYUN, DO-SEONG;LEE, JOOYOUNG
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.22 no.3
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    • pp.57-68
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    • 2017
  • Continuous monitoring of spatial and temporal changes in key marine environmental parameters such as SST (sea surface temperature) near IORS (Ieodo Ocean Research Station) is demanded to investigate the ocean ecosystem, climate change, and sea-air interaction processes. In this study, we aimed to develop the system for continuously measuring SST using a TIR (thermal infrared) sensor mounted at the IORS. New SST algorithm is developed to provide SST of better quality that includes automatic atmospheric correction and emissivity calculation for different oceanic conditions. Then, the TIR-based SST products were validated against in-situ water temperature measurements during May 17-26, 2015 and July 15-18, 2015 at the IORS, yielding the accuracy of 0.72-0.85 R-square, and $0.37-0.90^{\circ}C$ RMSE. This TIR-based SST observing system can be installed easily at similar Ocean Research Stations such as Sinan Gageocho and Ongjin Socheongcho, which provide a vision to be utilized as calibration site for SST remotely sensed from satellites to be launched in future.