• Title/Summary/Keyword: In situ method

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A Comparison Method of Silver Nanoparticles Prepared by the Gamma Irradiation and in situ Reduction Methods

  • Lee, Chul-Jae;Karim, Mohammad Rezaul;Vasudevan, T.;Kim, Hee-Jin;Raushan, K.;Jung, Maeng-Joon;Kim, Dong-Yeub;Lee, Mu-Sang
    • Bulletin of the Korean Chemical Society
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    • 제31권7호
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    • pp.1993-1996
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    • 2010
  • Silver nanoparticles has been prepared by the $\gamma$-irradiation and in situ reduction methods. Based on the Raman spectra, TEM images, X-ray Diffraction (XRD) patterns and UV-vis spectra, the in situ reduction method is more stable and the average size of the silver nanoparticles is also smaller than by the $\gamma$-irradiation reduction method. It is identified that the silver ions interacting with nonbonding electrons of oxygen atom in the carbonyl group of polyvinylpyrrolidone (PVP) by the in situ reduction method. It is also found advantages of the in situ reduction method including no additional reducing agents, without $\gamma$-irradiations treatment and the room temperature treatment suitability.

고지의 광학적특성 개선을 위한 in-situ 탄산칼슘처리기술의 적용 (Application of in-situ CaCO3 forming process on recycled fibers for optical property improvement)

  • 박동휘;이민우;이종규;안지환;서영범
    • 펄프종이기술
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    • 제44권4호
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    • pp.8-15
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    • 2012
  • Optical property improvements for ONP (old newspaper) and OMG (old magazine) were attempted by application of in-situ $CaCO_3$ formation process on recycled fiber surfaces. Washing treatment of ONP and OMG resulted in 35~40% yield loss for around 6% brightness improvement. Washing plus bleaching process with $H_2O_2$ and FAS (formamidine sulfinic acid) improved brightness and ERIC values a little more with the same amount of yield loss as washing treatment. In-situ $CaCO_3$ formation method improved those optical properties much better than the washing plus bleaching method without loss of yield, and better than the case of adding high brightness PCC up to the same ash level. It can be said that the in-situ $CaCO_3$ formation method may be used as an effective alternative for upgrading optical properties of recycled fibers.

In-situ 광배향법을 이용한 Flexible TN 셀의 액정배향 효과 (Liquid Crystal Alignment Effect using in-situ Photoalignment on Flexible TN cell)

  • 남기형;황정연;이휘원;최면길;서동학;김영환;서대식
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2004년도 추계학술대회 논문집 Vol.17
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    • pp.506-509
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    • 2004
  • We have investigated the generation of pretilt angle for a nematic liquid crystal (NLC) alignment with in-situ photoalignment method on polyimide (PI) surfaces using thin plastic substrates. The LC aligning capabilities and pretilt angle of the thin plastic substrates by in-situ photoalignment method were better than that of the glass substrate by general photoalignment. Also, the LC pretilt angle increased with increasing heating temperature and exposure time. And EO characteristics of photoaligned TN-LCDs using in-situ photodissociation method on glass substrate and on plastic substrate are also excellent.

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In-situ 진공게이지 교정장치 개발 (Development of an Apparatus for In-situ Vacuum Gauge Calibration)

  • 홍승수;임인태;조문재;정원호
    • 한국진공학회지
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    • 제15권6호
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    • pp.605-611
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    • 2006
  • 정적법(constant volume method)을 이용하여 $1Pa\sim100kPa$ 영역에서 진공게이지의 직접교정 (direct calibration) 장치를 개발하였다. 이 장치는 피 교정 게이지의 이동 없이 in-situ 상태에서 비교교정(comparison calibration)도 가능하다. 이 장치가 개발됨으로서 산업체 교정기관의 생산성을 높이고 개발도상국에 국가 진공표준과 교정기술을 보급할 수 있게 되었다.

원전 고온 1차수 환경에서 응력부식균열의 실시간 마이크로 스케일 관찰 방법 개발 (Development of Method for In-situ Micro-Scale Observation of Stress Corrosion Cracking in High-Temperature Primary Water Environment)

  • 신정호;이종연;김성우
    • Corrosion Science and Technology
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    • 제22권4호
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    • pp.265-272
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    • 2023
  • The aim of this study was to develop a new in-situ observation method and instrument in micro-scale to investigate the mechanism of stress corrosion cracking (SCC) initiation of Ni-base alloys in a high temperature water environment of pressurized water reactors (PWRs). A laser confocal microscope (LCM), an autoclave with diamond window view port, and a slow strain-rate tester with primary water circulation loop system were components of the instrument. Diamond window, one of the core components of the instrument, was selected based on its optical, chemical, and mechanical properties. LCM was used to observe the specimen in micro-scale, considering the experimental condition of a high-temperature primary water environment. Using in-situ method and instrument, it is possible to observe oxidation and deformation of specimen surface in micro-scale through the diamond window in a high-temperature primary water in real-time. The in-situ method and instrument developed in this work can be utilized to investigate effects of various factors on SCC initiation in a high-temperature water environment.

Experimental Applications of in situ Liver Perfusion Machinery for the Study of Liver Disease

  • Choi, Won-Mook;Eun, Hyuk Soo;Lee, Young-Sun;Kim, Sun Jun;Kim, Myung-Ho;Lee, Jun-Hee;Shim, Young-Ri;Kim, Hee-Hoon;Kim, Ye Eun;Yi, Hyon-Seung;Jeong, Won-Il
    • Molecules and Cells
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    • 제42권1호
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    • pp.45-55
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    • 2019
  • The liver is involved in a wide range of activities in vertebrates and some other animals, including metabolism, protein synthesis, detoxification, and the immune system. Until now, various methods have been devised to study liver diseases; however, each method has its own limitations. In situ liver perfusion machinery, originally developed in rats, has been successfully adapted to mice, enabling the study of liver diseases. Here we describe the protocol, which is a simple but widely applicable method for investigating the liver diseases. The liver is perfused in situ by cannulation of the portal vein and suprahepatic inferior vena cava (IVC), with antegrade closed circuit circulation completed by clamping the infrahepatic IVC. In situ liver perfusion can be utilized to evaluate immune cell migration and function, hemodynamics and related cellular reactions in each type of hepatic cells, and the metabolism of toxic or other compounds by changing the composition of the circulating media. In situ liver perfusion method maintains liver function and cell viability for up to 2 h. This study also describes an optional protocol using density-gradient centrifugation for the separation of different types of hepatic cells, allowing the determination of changes in each cell type. In summary, this method of in situ liver perfusion will be useful for studying liver diseases as a complement to other established methods.

UV 배향된 액정셀에서의 전기광학 특성 (Electro-Optical Characteristics of the UV Aligned LCD Cell)

  • 이병순;이호영
    • 전기학회논문지P
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    • 제62권4호
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    • pp.223-226
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    • 2013
  • In this study, we investigated the electro-optical(EO) characteristic of fringe-field switching(FFS) mode cell by the two kinds of ultraviolet(UV) alignment method on the organic thin film(polyimide: PI). The suitable organic layersfor FFS cell and the aligning capabilities of nematic liquid crystal(NLC) using the in-situ photoalignment method were studied Disclination is observed after conventional photoalignment method for 1h, and in-situ photoalignment method for 1h. Monodomain alignment of the NLC can be observed via in-situ photoalignment method for 2h and 3h. It is considered that NLC alignment is due to photo-depolymerization of the polymer with oblique non-polarized UV irradiation on PI surface. An unstable V-T curve of UV-aligned FFS-LCD with conventional photoalignment method can be achieved. However, a stable V-T curve of UV-aligned FFS-LCD with in-situ photoalignment method(1h), and V-T curve of UV-aligned FFS-LCD with in-situphotoalignment method was much stable comparing with that of other UV-aligned FFS-LCD's. As a result, more stable EO performanceof UV-aligned FFS-LCD with in-situphotoalignment method for 3h is obtained than that of the other UV-aligned FFS-LCD's.

저온 Si계 에피 성장기술에서 실험계획법에 의한 in-situ H$_2$ bake 및 GeH$_4$ clean 공정 최적화 (The process optimization of in-situ H$_2$ bake and GeH$_4$ clean in low temperature Si epitaxy using design of experiment)

  • 이경수
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 1994년도 추계학술대회 논문집
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    • pp.54-58
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    • 1994
  • H$_2$ bake and GeH$_4$ clean are used as a in-situ pre-clean method in low temperature Si based epitaxial growth technology using rapid thermal processing chemical vapor deposition (RTPCVD). In this paper, the H$_2$ bake and GeH$_4$ clean processes are optimized for low surface defect density using Taguchi method. In H$_2$ bake process, the epitaxial growth temperature affects dominantly on the surface defect density, and the next affecting factors are H$_2$ bake temperature and rinse time in de-ionised water. In GeH$_4$ clean process, GeH$_4$ clean temperature affects most strongly on the surface defect density, and the minor factor is GeH$_4$flow rate. The optimum process conditions predicted fly Taguchi method agree well with tile experimental data in both in-situ clean processes.

LSM을 사용한 합성 PC 부재의 현장생산 공기 산정 기초연구 (Basic study for time analysis of insitu production of composite precast concrete members using linear scheduling method)

  • 임채연;김선국
    • 한국건축시공학회:학술대회논문집
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    • 한국건축시공학회 2014년도 추계 학술논문 발표대회
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    • pp.92-93
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    • 2014
  • Green Frame is a method for Rahmen structure construction composed of composite PC members. The composite PC members of Green Frame which are based on in-situ production can reduce the construction cost and are more likely to secure quality when compared to production in factories. Previous studies developed forms for in-situ production of Green Frame composite PC members and proposed algorithms to arrange them on site. However, it requires not only their arrangement, but also calculation of an accurate production period to produce the required PC members in a limited space and supply them in a timely manner. In particular, it is necessary to clearly define the properties of detailed processes for in-situ production of PC members and to calculate the time required for respective process. To do so, this study is a basic research on calculating the time for in-situ production using a linear scheduling method.

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In situ PCR에 의한 alcelaphine herpesvirus-l (AHV-l)의 진단법 개발 및 다른 분자생물학적 진단법들과의 비교 (In situ PCR for the Detection of Alcelaphine Herpesvirus-l and Comparison with other Molecular Biological Diagnostic Methods)

  • 김옥진
    • 한국수의병리학회지
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    • 제6권1호
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    • pp.1-5
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    • 2002
  • A1celaphine herpesvirus 1 (AHV-1) is a causative agent of malignant catarrhal fever which is a fatal and a lymphoproliferative syndrome. AHV-1 is a gamma herpesvirus, which induces frequent latent infection and often difficult to detect its antigens or specific nucleic acids because of its low viral copies in the infected tissues. A new method, in situ PCR, is developed for the detection of AHV-1 nucleic acid in this study. Target sequences of AHV-1 open reading frame 50 gene were detected within AHV-1 infected MDBK cells. As compare with other molecular biological methods for the detection of AHV-1, in situ PCR was found to be more sensitive than in situ hybridization and to be less sensitive than nested PCR. However, nested PCR cannot afford to observe and differentiate AHV-1 infected cells. In situ PCR amplifies a target sequence within cells that can be visualized microscopically with increased sensitivity compared to detection by in situ hybridization. In situ PCR has wide applications for sensitive localization of low copy AHV-1 viral sequences within cells to investigate the role of viruses in a variety of clinical conditions and also provide the rapid, sensitive, and specific detection of AHV-1 infection.

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