• Korean Journal of Plant Resources
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• v.25 no.4
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• pp.407-415
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• 2012

Pinus pumila, which occurs in the northeast Asia, is found limitedly in Daecheongbong area of Mt. Seorak in the South Korea. This population was chosen to study spatial pattern, genetic diversity and spatial genetic structure. There were 48 polymorphic and 30 monomorphic I-SSR markers. A total of 65 individuals which distributed in the study site (40 m ${\times}$ 70 m) showed weakly aggregate distribution (Aggregate Index = 0.871). A total of 40 genets were observed from 65 individuals through I-SSR genotype comparison. Proportion of distinguishable genotype (G/N), genotype diversity (D) and genotype evenness (E) were 61.5%, 0.977 and 0.909, respectively. In spite of the small number and the limited distribution, Shannon's diversity index (I = 0.567) was relatively high as compared with those of other plant species. Spatial autocorrelation using Tanimoto's distance showed that the genetic patch was established within 12 m. Based on Mantel tests, there was relatively low correlation between genetic distance and geographic distance. Therefore, it seems the P. pumila population was formed by many parent trees in early stage. For ex situ genetic conservation of P. pumila, the sampling strategy is efficient at least above 12 m between individual trees.

• Journal of Korea Water Resources Association
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• v.54 no.3
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• pp.167-179
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• 2021

In order to characterize the mixing process of confluence for understanding the impacts of a river on the other river, it has been crucial to analyze the spatial mixing patterns for main streams depending on various inflow conditions of tributaries. However, most conventional studies have mostly relied upon hydraulic or water quality numerical models for understanding mixing pattern analysis of confluences, due to the difficulties to acquire a wide spatial range of in-situ data for characterizing mixing process. In this study, backscatters (or SNR) measured from ADCPs were particularly used to track sediment mixing assuming that it could be a surrogate to estimate the suspended sediment concentration. Raw backscatter data were corrected by considering the beam spreading and absorption by water. Also, an optical Laser diffraction instrument (LISST) was used to verify the method of acoustic backscatter and to collect the particle size distribution of main stream and tributary. In addition, image-based spatial distributions of sediment mixture in the confluence were monitored in various flow conditions by using an unmanned aerial vehicle (UAV), which were compared with the spatial distribution of acoustic backscatter. As results, we found that when acoustic backscatter by ADCPs were well processed, they could be proper indicators to identify the spatial patterns of the three-dimensional mixing process between two rivers. For this study, flow and sediment mixing characteristics were investigated in the confluence between Nakdong and Nam river.

• Journal of the Korea Organic Resources Recycling Association
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• v.25 no.1
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• pp.57-65
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• 2017

In a column experiment with low rank coal, rice straw was additionally supplied to induce methane gas generation by microorganisms in the state of supplying microorganisms and nutrients, and long-term biogas production characteristics were observed. When the weight ratio of the rice straw to coal was 0.04 or less, there was no significant gas generation. At 0.08, the biogas was generated for about 90 days. However, the methane gas generation was only 5% compared with the vial test result at optimum condition. Therefore, in order to produce biogas in the coal deposit in situ, a reactor that operates at COD concentration of 2000 mg/L or more at a ratio of 1:3 or more of rice straw to coal should be installed on the ground or under the ground. Liquid from the column filled with coal and rice straw and a liquid from vial containing rice straw were analyzed by microbial community analysis using pyrosequencing method, and compared the dominant microbial species among the two samples. In terms of the uniformity and diversity of the bacteria, the coal-filled column showed various species distribution, which has shown to be a disadvantageous microbial distribution to methane production.

• Journal of Korean Society of Forest Science
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• v.96 no.6
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• pp.667-675
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• 2007

The genetic diversity and the spatial structure in two populations of Abelia tyaihyoni in Yeongwol region were studied by employing I-SSR markers. In spite of the limited distribution and small population sizes of Abelia tyaihyoni, the amount of genetic diversity estimated at the individual level was comparable to other shrub species (S.I.=0.336, h=0.217). Genetic diversity at the genet level was very similar to that at individual level. (S.l.=0.339, h=0.219). About 18.7 percent of total variation was allocated between two populations, which was slightly higher or similar level as compared with other shrub species. Genotypic diversity estimated by the ratio of the number of genets ($N_G$) over the total number of individuals (N) and a modified Simpson's index ($D_G$) were also higher than those of other shrubs. The maximum diameter of a genet did not exceed 5.5 m. The high level of gene and genotypic diversity, and the relatively limited maximum diameter of a genet suggested that the clonal propagation is not the most dominant factor in determining the population structure of Abelia tyaihyoni. Spatial autocorrelation analysis revealed significant spatial genetic structure within 12 m and 18 m distances in two populations A and B, respectively. Autocorrelations among individuals at the both individual and genet levels in each population didn't show any considerable differences. As a sampling strategy for ex-situ conservation of populations showing continuous distribution, a minimum distance of 18 m between individuals was recommended. For the populations with many segments, it was considered very crucial to sample materials from as many segments as possible.

• Journal of Korea Water Resources Association
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• v.53 no.11
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• pp.973-983
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• 2020

Recently, SAR (Synthetic Aperture Radar) is being highlighted as a solution to the coarse spatial resolution of remote sensing data in water resources research field. Spatial resolution up to 10 m of SAR backscattering coefficient has facilitated more elaborate analyses of the spatial distribution of soil moisture, compared to existing satellite-based coarse resolution (>10 km) soil moisture data. It is essential, however, to multilaterally analyze how various hydrological and environmental factors affect the backscattering coefficient, to utilize the data. In this study, soil moisture estimated by WCM (Water Cloud Model) and linear regression is compared with in-situ soil moisture data at 5 soil moisture observatories in the Korean peninsula. WCM shows suitable estimates for observing instant changes in soil moisture. However, it needs to be adjusted in terms of errors. Soil moisture estimated from linear regression shows a stable error range, but it cannot capture instant changes. The result also shows that the effect of soil moisture on backscattering coefficients differs greatly by land cover, distribution of vegetation, and water content of vegetation, hence that there're still limitations to apply preexisting models directly. Therefore, it is crucial to analyze variable effects from different environments and establish suitable soil moisture model, to apply SAR to water resources fields in Korea.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.15 no.1
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• pp.1-10
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• 2003

In this paper, the laboratory test results with middle-sired ,soil box test in modeling the in-situ installing of horizontal drains are discussed the estimation of the optimum negative pressure. The test was carried out in the different vacuum pressure conditions together with the measurement for the settlement and volume change of drained water by the installed drains during the consolidation process. After the test, the water content was measured to both directions of lateral distance from the drain and depth of the soil, to find out the distribution of ground improvement and strength enhancement. From the analysis on the distribution of water content, the gradual application of vacuum pressure to higher level by pre-determined stages starting from low vacuum pressure is found to be effective and desirable. In the comparison of the degrees of consolidation with elapsed time, the calculated value by the prediction method based on the Barren's conventional theory showed a good agreement with the measured value. With this, It is positively considered that the applicability of the prediction method based on Barren's theory to the practical design of horizontal drains can be justified such as in the calculation of drain spacing and consolidation period.

• Journal of Environmental Impact Assessment
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• v.23 no.2
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• pp.119-136
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• 2014

This work aims to select the potentially vulnerable plant species against climate change at alpine and subalpine belts of Mts. Sorak, Jiri, and Halla, from central, southern, southern insular high mountains of the Korean Peninsula, respectively. The selection of global warming related vulnerable plants were performed by adapting various criteria, such as flora, endemicity, rarity, floristically specific and valuable species, species composition at mountain summits, horizontal and vertical ranges of individual species, and their distributional pattern in the Korean Peninsula. Line and quadrat field surveys along the major trails from all directions at height above 1,500 meters above sea level of Mts, Sorak, Jiri and Halla were conducted each year during spring, summer, and autumn from 2010 to 2011. Based upon above mentioned eight criteria, high level of climate change related potentially vulnerable arboral plants, such as Rhododendron aureum, Taxus caespitosa, Pinus pumila, Oplopanax elatus, Vaccinium uliginosum, and Thuja koraiensis are noticed from at subalpine belt of Mt. Sorak. Species of Abies koreana, Rhododendron tschonoskii, Oplopanax elatus, Taxus cuspidata, Picea jezoensis, and Juniperus chinensis var. sargentii belong to climate change concerned vulnerable species at subalpine belt of Mt. Jiri. High level of climate change related species vulnerability is found at alpine and subalpine belts of Mt. Halla from Diapensia lapponica var. obovata, Salix blinii, Empetrum nigrum var. japonicum, Vaccinium uliginosum, Juniperus chinensis var. sargentii, Taxus cuspidata, Rhamnus taquetii, Abies koreana, Hugeria japonica, Prunus buergeriana, and Berberis amurensis var. quelpartensis. Countermeasures to save the global warming vulnerable plants in situ are required.

• Proceedings of the KSRS Conference
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• 2008.10a
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• pp.344-347
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• 2008

Ocean surface waves may be modified by ocean current and their observation may be severely distorted if the observer is on a moving platform with changing speed. Tidal current near a sill varies inversely with the water depth, and results spatially inhomogeneous modulation on the surface waves near the sill. For waves propagating upstream, they will encounter stronger current before reaching the sill, and therefore, they will shorten their wavelength with frequency unchanged, increase its amplitude, and it may break if the wave height is larger than 1/7 of the wavelength. These small scale (${\sim}$ 1 km changes is not suitable for satellite radar observation. Spatial distribution of wave-height spectra S(x, y) can not be acquired from wave gauges that are designed for collecting 2-D wave spectra at fixed locations, nor from satellite radar image which is more suitable for observing long swells. Optical images collected from cameras on-board a ship, over high-ground, or onboard an unmanned auto-piloting vehicle (UAV) may have pixel size that is small enough to resolve decimeter-scale short gravity waves. If diffuse sky light is the only source of lighting and it is uniform in camera-viewing directions, then the image intensity is proportional to the surface reflectance R(x, y) of diffuse light, and R is directly related to the surface slope. The slope spectrum and wave-height spectra S(x, y) may then be derived from R(x, y). The results are compared with the in situ measurement of wave spectra over Keelung Sill from a research vessel. The application of this method is for analysis and interpretation of satellite images on studies of current and wave interaction that often require fine scale information of wave-height spectra S(x, y) that changes dynamically with time and space.

• Ocean and Polar Research
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• v.27 no.2
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• pp.205-214
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• 2005

From ancient Antarctic glacier ice, we extracted total genomic DNA that was suitable for prokaryotic 16S rDNA gene cloning and sequencing, and bacterial artificial chromosome (BAC) library and end-sequencing. The ice samples were from the Dry Valley region. Age dating by $^{40}Ar/^{39}Ar$ analysis on the volcanic ashes deposited in situ indicated the ice samples are minimum 100,000-300,000 yr (sample DLE) and 8 million years (sample EME) old. Further assay proved the ice survived freeze-thaw cycles or other re-working processes. EME, which was from a small lobe of the basal Taylor glacier, is the oldest known ice on Earth. Microorganisms, preserved frozen in glacier ice and isolated from the rest of the world over a geological time scale, can provide valuable data or insight for the diversity, distribution, survival strategy, and evolutionary relationships to the extant relatives. From the 16S gene cloning study, we detected no PCR amplicons with Archaea-specific primers, however we found many phylotypes belonging to Bacteria divisions, such as Actinobacteria, Acidobacteria, Proteobacteria $({\alpha},\;{\beta},\;and\;{\gamma})$, Firmicutes, and Cytophaga-Flavobacterium-Bacteroid$. BAC cloning and sequencing revealed protein codings highly identical to phenylacetic acid degradation protein paaA, chromosome segregation ATPases, or cold shock protein B of present day bacteria. Throughput sequencing of the BAC clones is underway. Viable and culturable cells were recovered from the DLE sample, and characterized by their 16S rDNA sequences. Further investigation on the survivorship and functional genes from the past should help unveil the evolution of life on Earth, or elsewhere, if any.

• Journal of the Mineralogical Society of Korea
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• v.24 no.3
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• pp.217-224
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• 2011

Removal of dissolved selenium by D. michiganensis, a iron-reducing bacterium, and effects of dissolved metal elements such as iron, sulfate, and copper were investigated. Selenide that was reduced from selenite (2 mM) by D. michiganensis was gradually removed from the aqueous medium. As the reduced selenide was combined with aqueous iron, it was precipitated as a nanoparticulate iron-selenide. Sulfate and copper negatively affected the microbial selenite reduction, and the copper was especially toxic to the bacterium, inhibiting a microbial removal of dissolved selenite. These results show that it should be carefully biotreated for a selenium-contaminated site considering in situ sulfate or copper distribution and concentration. Consequently, the formation of iron-selenide by bacteria will be an important measure for preventing a long-distance migration of selenium in the subsurface environments.