• Asian-Australasian Journal of Animal Sciences
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• v.17 no.5
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• pp.612-616
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• 2004

This study was carried out to compare the semen characteristics, frozen-thawed sperm viability and testosterone concentration and in vitro fertilization (IVF) and development of in vitro matured pig oocytes between two Yorkshire boars. Semen and blood samples were collected once per week from October to November 2002 from two adult Yorkshire boars at 18 months of age with 170 kg body weight. Sperm were deep frozen in 5 ml maxi-straws with lactose-egg yolk and N-acetyl-D-glucosamine (LEN) diluent and stored in liquid nitrogen. Blood samples were obtained at 10 a.m. by inserting a 21 gauge, hypodermic needle attached to 10 ml syringe into surface veins in the ear. The concentration of testosterone was determined by Competitive Enzyme Immunoassay. Ovaries were collected from prepubertal gilts at a local slaughter house. Cumulus oocyte complexes were aspirated from antral follicles (3 to 6 mm in diameter). The medium used for oocyte maturation was modified TCM 199. After about 22 h of culture, oocytes were cultured without cysteamine and hormones for 22 h at $38.5^{\circ}C$, 5% $CO_2$ in air. For IVF, one frozen 5 ml straw was thawed at $52^{\circ}C$in 40 sec and was diluted with 20 ml Beltsville thawing solution at room temperature. Sperm were washed 2 times in mTLP-PVA and inseminated without preincubation after thawing. Oocytes were inseminated with $2{\times}10^7$/ml sperm concentration. Oocytes were coincubated for 6 h in 500 ${\mu}$l mTBM fertilization medium. At 6 h after IVF, oocytes were transferred into 500 ${\mu}$l NCSU-23 culture medium for further culture of 48 and 144 h. There were no significant differences in the semen volume, motility, normal acrosome morphology and sperm concentration of raw semen between A and B of Yorkshire boar. However, motility and normal acrosome of boar A were higher than those of boar B at 0.5, 2, 3, 4, 5 and 6 h incubations of frozen-thawed sperm. Testosterone concentration (3.75 ng/ml) of boar A was higher than that (2.34 ng/ml) of boar B. The rate of blastocyst formation (15.1%) of boar A was higher than that (10.4%) of boar B. In conclusion, serum testosterone concentration of boar showed very important role for the frozen-thawed sperm viability and the blastocyst formation of pig oocytes matured in vitro.

• The Journal of Information Systems
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• v.27 no.1
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• pp.171-191
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• 2018

Purpose Corporate technology leakage is not only monetary loss, but also has a negative impact on the corporate image and further deteriorates sustainable growth. In particular, since SMEs are highly dependent on core technologies compared to large corporations, loss of technology leakage threatens corporate survival. Therefore, it is important for SMEs to "prevent and protect technology leakage". With the recent development of data analysis technology and the opening of public data, it has become possible to discover and proactively detect companies with a high probability of technology leakage based on actual company data. In this study, we try to construct profiles of enterprises with and without technology leakage experience through profiling analysis using data mining techniques. Furthermore, based on this, we propose a classification model that distinguishes companies that are likely to leak technology. Design/methodology/approach This study tries to develop the empirical model for prevention and protection of technology leakage through profiling method which analyzes each SME from the viewpoint of individual. Based on the previous research, we tried to classify many characteristics of SMEs into six categories and to identify the factors influencing the technology leakage of SMEs from the enterprise point of view. Specifically, we divided the 29 SME characteristics into the following six categories: 'firm characteristics', 'organizational characteristics', 'technical characteristics', 'relational characteristics', 'financial characteristics', and 'enterprise core competencies'. Each characteristic was extracted from the questionnaire data of 'Survey of Small and Medium Enterprises Technology' carried out annually by the Government of the Republic of Korea. Since the number of SMEs with experience of technology leakage in questionnaire data was significantly smaller than the other, we made a 1: 1 correspondence with each sample through mixed sampling. We conducted profiling of companies with and without technology leakage experience using decision-tree technique for research data, and derived meaningful variables that can distinguish the two. Then, empirical model for prevention and protection of technology leakage was developed through discriminant analysis and logistic regression analysis. Findings Profiling analysis shows that technology novelty, enterprise technology group, number of intellectual property registrations, product life cycle, technology development infrastructure level(absence of dedicated organization), enterprise core competency(design) and enterprise core competency(process design) help us find SME's technology leakage. We developed the two empirical model for prevention and protection of technology leakage in SMEs using discriminant analysis and logistic regression analysis, and each hit ratio is 65%(discriminant analysis) and 67%(logistic regression analysis).

• Journal of Korean Academy of Nursing
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• v.29 no.6
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• pp.1365-1378
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• 1999

This study is a methodological research study to develop an instrument to measure in patients with cancer and to test the validity and reliability of the instrument. The research procedure was as follows : 1) The first step was to develop conceptual framework based on a comprehensive review of the literature and in-depth interviews with patients with cancer. This conceptual framework was organized in to three dimensions (the intrapersonal dimension, the significant-other and context related dimension, the transcendental dimension). Initially 59 items were adopted. 2) These items were analyzed through the index of content validity(CVI) and 53 items were selected which met more than 80% on the CVI. 3) The pretest was carried out with 87 patients with cancer. After the pretest results were analyzed by item analysis, 44 items were selected. A second test of content validity was conducted and 6 items were eliminated considering the 80% CVI. 4) To test for reliability and validity, data collection was done during the period from January 25, 1999, to February 26, 1999. The subjects for the test were 160 patients with cancer and 185 healthy persons. analysis, item analysis and multitrait-multimethod method to analyze validity. The findings are as follows : 1) The Cronbach's alpha coefficient for internal consistency was .92 for the total 38 items and .79, .82, .85, for the three dimensions in that order. 2) The item analysis was based on the corrected item to total correlation coefficient( .30 or more) and information about the alpha estimate if this item was dropped from the scale. 3) As a result of the initial factor analysis using principal component analysis and varimax rotation, one item was deleted because of factor complexity (indiscriminate factor loadings). In the secondary factor analysis, 7 factors with eigenvalue of more than 1.0 were extracted and these factors explained 56 percents of the total variance. The seven factors were labeled as 'family relationship', 'emotional condition', 'physical discomfort', 'meaning and goal of life', 'contextual stimuli', 'change of body image', 'guilt feelings'. 4) The convergence effect between this instrument and the life satisfaction scale was identified and there was significant positive correlation(r= .52, p= .00). The discriminant validity between this instrument and the depression scale(CES-D) was tested and there was significant negative correlation(r= -.50, p= .00). The instrument for accessing the suffering of patients with cancer developed in this study was identified as a tool with a high degree of reliability and validity. In this sense, this tool can be effectively utilized for assessment in caring for patients with cancer.

• Journal of Korea Multimedia Society
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• v.11 no.9
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• pp.1204-1212
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• 2008

The vehicle simulation (flight simulator) in 1920's was the first in the virtual reality. With the development of precise optical and electromagnetic equipment, the virtual reality widened its application for other purposes than military one. Based on the realistic display technology, it is more and more common in the various areas such as entertainment, medical profession, learning, film, architectural design, tourism and etc. In 1989, Jaron Ranier was the first to use the terminology 'Virtual Reality'. With this term, all virtual projects could be classified in a single item. But even before the term was used, the virtual reality has been studied up to now. As a part of virtual reality, the human thirst for the impossible thing has led to the development of ride film. The ride film consists of the special technical elements as well as the psychological analysis of human being. The ultimate purpose of virtual reality is engrossment through interaction. Even though the real interaction requires interface, input sensor and reaction ability, the ride film is not an element of the typical interaction. The virtual reality is mostly defined in technical terms now. But in this study, we will analyze the concepts worked out by Professor Michael Haim who is called a philosopher in the cyberspace in aspect of experience-oriented definition. We will analyze the adaptability of virtual reality based on his concepts such as artificial reality/ interaction/ engrossment/ networked world/ remote display/ simulation/ onmon engrossment. And also, we aim to suggest the directions of developing the ride films for perfect engrossment and to draw optimized conclusion thereon. In this viewpoint, we consider that the study of ride film on which there were few case studies will contribute to level up the basic frameworks of IT technology and the digital image.

• Journal of the Korean Association of Geographic Information Studies
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• v.16 no.3
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• pp.136-146
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• 2013

This study has specified terminology for mountain ridgeline, and organized the present condition of Korean mountain ridgeline and empirical management measures of Beakdudeagan and Jeongmeak. Moreover, based on previously published paper maps, mountain map, and numerical maps, this study has used GIS to reorganize Jeongmaek of Korea, including Hanbukjeongmaek, Hannamjeongmaek, and Gumbukjoengmaek. To ensure accuracy, DEM, Shaded Relief Image, gradient, curvature, and aspect have been analyzed from recent numerical maps. On extraction process, this study analyzes more specifically the results, previous Beakdudaegan is modified and new Jeongmaeks have been extracted. Moreover, for analyzing natural environment of surrounding of major mountain ridgeline, ecological zoning grade of mountain ridgeline is analyzed. With the analyzed result, domestic and foreign mountain ridgeline management policy is suggested, and environmental impact assessment method for major mountain ridgeline development is also suggested. In this study, Baekdudaegan and its Jeongmaek are organized and analyzed. With the results, this study suggested political linkage of mountain ridgelines and guidelines for environmental impact assessment of ridgeline development.

• Archives of design research
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• v.19 no.4 s.66
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• pp.175-186
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• 2006

In the 21C, each nation controls exhaust fumes from automobiles and makes an effort to develop alternative energy because of serious environmental problem. Jechon area has many historical and cultural archeological sites. And Jechon city sponsors various cultural events. But the way of transportation which is connected with Jecheon and around sightseeing places is general and not ready yet. Therefore, if a special means of vehicle is developed, it could play an another role of sightseeing resources. Special identity of Jecheon area for establishment of green vehicle traffic system which gives Jecheon area specific character was investigated for theoretical background. Traffic system was studied for establishment of direction through existent successful case study. Moreover content, method, structure and advantage & shortcoming etc. of vehicle that use green energy resource such as solar car, fuel cell car, hybrid car, natural gas car etc. were examined. The suitable means of vehicle for Jechon area was proposed to three directions with research and investigation. After comparison and investigation by inquiry of each section's experts, the most suitable traffic system of which energy resource of car, form of vehicles, the complement, dimension of vehicles etc. were decided. Design proposal should be drawn according to process of automobile design in decided direction. Special Exterior design of vehicle that use green energy resource connecting Jecheon and around area should be suggested in Jecheon City Hall and Chungchong-bukdo provincial office for vivify image of cleanliness area.

• Journal of Biosystems Engineering
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• v.28 no.6
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• pp.517-524
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• 2003

There have been worldwide research and development efforts to automate various processes of bio-production and those efforts will be expanded with priority given to tasks which require high intensive labor or produce high value-added product and tasks under hostile environment. In the field of bio-production capabilities of the versatility and robustness of automated system have been major bottlenecks along with economical efficiency. This paper introduces a new concept of automation based on tole-operation, which can provide solutions to overcome inherent difficulties in automating bio-production processes. Operator(farmer), computer, and automatic machinery share their roles utilizing their maximum merits to accomplish given tasks successfully. Among processes of greenhouse watermelon cultivation tasks such as pruning, watering, pesticide application, and harvest with loading were chosen based on the required labor intensiveness and functional similarities to realize the proposed concept. The developed system was composed of 5 major hardware modules such as wireless remote monitoring and task control module, wireless remote image acquisition and data transmission module, gantry system equipped with 4 d.o.f. Cartesian type robotic manipulator, exchangeable modular type end-effectors, and guided watermelon loading and storage module. The system was operated through the graphic user interface using touch screen monitor and wireless data communication among operator, computer, and machine. The proposed system showed practical and feasible way of automation in the field of volatile bio-production process.

• Journal of the Korean association of regional geographers
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• v.3 no.2
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• pp.195-208
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• 1997

On the basis of the topographic maps in the 1910's and 1990's and the classification map of Landsat TM satellite image photographed on Sept 1, 1996, the spatial distribution and the current situation concerning tidal flats in Korea were studied by measuring the area with GIS Arc/Info system and examining the regional condition required to develop the tidal flats. The results are as follow; The tidal flat resources in Korea cover an area of about 3800 square meters, including the reclaimed one since the 1910's. And they are widely distributed in the west coast of South Jeonla, Kyunggi bay, Asan bay, the south coast of South Jeonla, Kunsan bay, Chunsoo bay, and the coast of South Kyungsang and Pusan when put in the order from bigger area. Given the area under the construction at present, more than 50% of the tidal flats are reclaimed ones. The tidal flats are being developed especially in Kyunggi and Asan bays because they perfectly measure up to the conditions required. For Kunsan bay, a remarkably good supply system of the alluvial sedimentary materials and a favorable coastline requirement for the coast of the South Jeonla also contribute to the development of the tidal flats. In the case study of Kunsan bay, it was shown that the shape of the tidal flat is making a continuous change and the area is getting bigger in terms of the multi-temporal change of the tidal flat development. However, while in the first half of the 20th century, the increasing rate of the area was considered to be rather high, it is considerably lowered almost to a standstill in the latter half of the century.

• Korean Journal of Clinical Laboratory Science
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• v.36 no.2
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• pp.163-172
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• 2004

Mast cells (MCs) have been implicated in the pathogenesis of tissue fibrosis. However, the role of MC in the development of liver fibrosis has not been fully elucidated. Stem cell factor (SCF) is known to recruit MCs to the liver following injury as it induces mast cell proliferation, survival and differentiation from resident tissue precursors. This study examines the interaction between activated hepatic stellate cells (HSCs) and MCs in rat fibrotic liver, and SCF production by HSCs during culture in vitro. Rats were studied 4, 7, 14 and 21 days after bile duct ligation (BDL). Fibrogenesis was assessed by a measurement of collagen stained with sirius red F3B. Activated HSCs and MCs were identified by ${\alpha}$-smooth muscle actin (${\alpha}-SMA$) immunohistochemical and alcian blue staining and measured by a computerized image analysis system. SCF production was determined in rat HSC cultures using Western blotting. Mild fibrotic changes were noted in BDL rat livers as early as 4 days after induction of cholestasis. Significant expansion and organization of fibrous tissue has occurred in day 14 BDL rats which progressed to bridging fibrosis by day 21. In BDL rats, both a large number of activated HSCs and MCs were detected in portal tracts and fibrous septa. Both area of activated HSCs infiltration and density of MCs were significantly higher in all BDL group compared with Shams. In BDL rats, both areas of activated HSCs infiltration and density of MCs were no significant difference between day 4 and 7 and were significantly higher in day 14. However, the areas of activated HSCs infiltration were significantly lesser in day 21 and the densities of MCs were significantly higher in day 21 compared with day14 BDL. In BDL rats, both areas of activated HSCs infiltration and density of MCs were highly correlated with areas of fibrosis. Western blotting showed that SCF protein was consistently produced in activated HSCs by culture on plastic and freshly isolated HSCs expressed relatively little 30kD SCF compared to late primary culture activated HSCs (day 14) and passaged HSCs. These results suggest that HSCs activated in vitro produce SCF, and may play an important role in recruiting mast cells to the liver during injury and fibrosis.

• Journal of the Korean Society for Marine Environment & Energy
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• v.17 no.3
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• pp.247-256
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• 2014

Recently, camera and RADAR are used for more effective and accurate observation of the bird migration. In recent years, many researches on the bird migration using RADAR are undertaking and in active, thus causes the advent of "RADAR ornithology" as a new academic field. Due to the lack of accessibility, economic feasibility and mobility of weather RADAR, airport searching RADAR and tracking RADAR, Nowadays, a marine RADAR is widely used for a bird observation. In this paper, we deals with a study on development of a remote bird observation system using marine FMCW RADAR, which monitors, records and analyzes bird movement by RADAR image processing and target recognition technology. Also, we conduct first test and second test for availability of the developed system, and verify the system to apply in bird observation domain. Consequently, we figured problems out, and correct the problems to improve the system. The developed system can apply in other domains such as environment evaluation. In the future, the system needs to improve accuracy of statistics and to track migration route of bird.