• Animal Systematics, Evolution and Diversity
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• v.33 no.2
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• pp.136-139
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• 2017

The genus Iksookimia (Actinopterygii: Cypriniformes: Cobitidae) is a bottom-dwelling freshwater loaches, which are well-known as their endemism and high geographic variation. However, population genetic relationships among Iksookimia spp. have remained unclear due to a shortage of genetic markers that can be applied generally in the genus. Here, we developed high-resolving microsatellite markers using I. koreensis and I. longicorpa as representatives of Iksookimia species because of their wide distribution range and phylogenetic position. Ten of polymorphic microsatellite loci were isolated from Iksookimia koreensis and were successfully cross-amplified in I. longicorpa. The mean number of observed alleles per locus was about 10.4 (range, 2-17) for I. koreensis and about 13.2 (range, 2-24) for I. longicorpa. The loci, IK03 and IK08, deviated from the Hardy-Weinberg equilibrium in I. koreensis, after applying the Bonferroni correction. The microsatellite markers obtained in the present study will be useful to evaluate population genetic structure and to establish conservation strategies for I. koreensis and related Iksookimia species.

• The Korean Journal of Zoology
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• v.39 no.4
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• pp.419-425
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• 1996

The five spedes of genus Iksookimia from Korea were investigated by electron microscopes to clarify the adhesive membranes on zona radiata. In the late vitellogenic stage the adhesive membranes could be classified into three form as follows: 1) villous form of Iksookimia koreensis, I pumila, and I. hugowolfeldi, 2) cotton-shaped form of I. longicorpus, and 3) vine-shaped form of I. choii. And although I. koreensis, I pumila, and I. hugowolfeldi possessed the same villous form, It was evident that fine structure of the zona radiata varied according to spedes. The adhesive membranes and fine structures of zona radiata in Iksookimla showed a species specificity related to closely their habitats and spawning properties.

• Korean Journal of Ichthyology
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• v.21 no.4
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• pp.253-261
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• 2009

Habitat environment and feeding habitat of Iksookimia koreensis and Cobitis lutheri were investigated in the Mangyeong River, Jeollabuk-do, Korea from 2005 to 2006. They together inhabit the upper and middle stream, but they showed differences in their microhabitat. I. koreensis inhabited the stony zones of relatively rapid waters with a water depth of 30~60 cm, whereas C. lutheri lived in the sandy zones of relatively slow waters with a depth of 30~100 cm. The two species were active during daylight hours from March to October but hibernated in the winter season. During hibernation I. koreensis still lived in the gravel and stone and C. lutheri burrowed in the sand. I. koreensis ingested mainly chironomid and other aquatic insects, whereas C. lutheri fed mainly on Chironomidae, Copepoda and Branchiopoda. The feeding rate of both species was highest in April and September, but they did not feed in the winter.

• Korean Journal of Ecology and Environment
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• v.45 no.1
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• pp.93-101
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• 2012

In the present study, egg development and early life history of Korean spined loach, $Iksookimia$ $koreensis$, were observed. Adult fish were sampled using spoon nets in Okgye-ri, Hoengseong-gun, Gangwon-do, Korea in July 2010. Eggs were obtained after injecting females with Ovarprim. Eggs were then artificially fertilized in the laboratory using the dry method. Mature eggs were slightly adhesive and transparent with a light yellowish color, and measured $1.40{\pm}0.04mm$ (mean${\pm}$SD) in diameter. Hatching of the embryo occurred approximately 50 h after fertilization in the water at $23^{\circ}C$, and newly hatched larvae were averaged $4.7{\pm}0.21mm$ in total length. 5 days after hatching, the averaged total length of larvae was $7.1{\pm}0.25mm$ and their yolk sacs had been completely absorbed. 17 days after hatching, fish started to enter the juvenile stage and reached $12.2{\pm}1.10mm$ in total length. 80 days after hatching, the band patterns and external form of juvenile fish were similar to those of adults, and they averaged $31.0{\pm}3.98mm$ in total length.

• Korean Journal of Environment and Ecology
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• v.16 no.4
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• pp.403-408
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• 2003

Fishfauna were investigated in the Baekdudaegan Mountain area (Manbokdae-siribong) streams. At the uppertributary of Seomjin River, 17 species of 5 families were collected and the dominant species was Zacco temminki. Korean endemic species were six (35.3%) species, Acheiognathus koreensis, Coreoleuciscus splendidus, Squalidus gruacilis majimae, Microphysogobio yaluensis, Iksookimia longicorpus, and Odontobutis platycephala. Only Lampetra reissneri designated as a vulnerable species, was confirmed at the stream. At the upper streams of the Nakdong River, collected fishes were identified as 11 species of 3 families. The dominant species was Zacco temmincki, and two species (18.2%), Squalidus gracilis. majiimae and Ikosokimia longicorpus were Korean endemic.

• Proceedings of the Korean Aquaculture Society Conference
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• 2006.05a
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• pp.562-563
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• 2006

• Korean Journal of Ichthyology
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• v.17 no.4
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• pp.271-278
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• 2005

The ichthyofauna and fish community were studied in Miho Stream from May 2004 to August 2005. During the surveyed period 41 species belonging to 10 families were collected. Twelve of the species (29.3%) are endemic to Korea. Exotic fishes were Carassius cuvieri, Lepomis macrochirus, and Micropterus salmoides. Dominant species was Zacco platypus (54.7%). Comparing the present result with that of Son (1982), the indices of diversity,evenness and richness were lower and the dominance index was higher; Lampetra reissneri, Anguilla japonica, Acheilognathus macropterus, Coreoleuciscus splendidus, Pseudopungtungia nigra, Gobiobotia naktongensis, Lefua costata, Pseudobagrus koreanus, Monopterus albus and Iksookimia choii did not appear; Carassius auratus, Rhodeus uyekii, Rhodeus notatus, Acheilognathus lanceolatus, Pseudogobio esocinus, Hemibarbus longirostris, Microphysogobio yaluensis, Cobitis lutheri, Iksookimia koreensis, Pseudobagrus fulvidraco, and Rhinogobius brunneus, remarkably decreased, and Z. platypus, Pseudorasbora parva, and Squalidus gracilis majimae increased.

• Korean Journal of Environment and Ecology
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• v.19 no.1
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• pp.46-54
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• 2005

The change of fish community in the Wonju-stream was investigated from May to November, 2004. During the period,24 species belonging to 6 families were collected. There were 10 Korean endemic species$(41.7\%)$, including Pseudopungtungia tenuicorpa, Coreoleuciscus spiendidus, Squalidus gracitis majimae, Squalidus japonicus coreanus, Microphysogobio yaluensis, Microphysogobio longidorsalis, Iksookimia koreensis, Koreocobitis rotundicaudata, Liobagus andersoni and Odontobutis interrupts. The dominant species was Zacco Platypus$(54.7\%)$, and the subdominant species was Rhynchocypris oxycephalus$(16.7\%)$. Also, Carassius auratus$(5.4\%)$, I. koreensis$(3.4\%)$, Orthrias toni$(3.3\%)$, and Pungtungia herzi$(3.0\%)$ appeared. According to the fish species compositions, the fish community was divided into 2 groups by an unweighted pair group method using arithmetic averages(UPGMA). The comparison of the fish community showed that benthic fish species were decreased, while, the number of water-column species, resistant 1,o substrate changes and water pollution were increased.

• Korean Journal of Ichthyology
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• v.20 no.1
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• pp.21-27
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• 2008

A total sample of 215 specimens of the Iksookimia koreensis was collected monthly from June 2004 to June 2005 in the Namdae stream, Cheorwon-gun, Gangwon-do, Korea. The age of every individual sample was estimated by the number of annual rings on the scales. A significant decrease of gonadosomatic index was noted between May and June reflecting the reproduction during this period. I. koreensis exhibited a strong bias to female (female : male, 1 : 0.56). The maximum age observed was 3 years old for male & female, both. The von Bertalanffy growth curves were expressed as $L_t=126.30[1-\exp\{-0.576(t+0.940)\}]$ for females and $L_t=100.19[1-\exp\{-1.119(t+0.695)\}]$ for males.Growth curve of weight was expressed as $W_t=11.567[1-\exp\{-0.576(t+0.940)\}]^{3.199}$ for females, $W_t=5.514[1-\exp\{-1.119(t+0.695)\}]^{3.199}$ for males. On the other hand, a significant difference in the growth rate between both sexes was found(i.e. females grew faster than males).

• Korean Journal of Environment and Ecology
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• v.25 no.1
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• pp.65-70
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• 2011

The fish fauna and population of Zacco koreanus in the upper region of the Gapyeong Stream was investigation from April, 2005 to August, 2009. The collected species during the survey period were 16 species belonging to 6 families. Dominant species was Z. koreanus and subdominant species was Pungtungia herzi. korea endemic species were Pseudopungtungia tenuicorpa, Coreoleuciscus splendidus, Hemibarbus mylodon, Gobiobotia brevibarba, Microphysogobio longidorsalls, Z. koreanus, Koreocobitis rotundicaudata, Iksookimia koreensis, Silorus microdorsalis, Liobagrus andersoni, Coreoperca herzi and Odontobutis platycephala, which showed a ratio of 75 % in all collected species. The species of H. mylodon was identified as natural monument, and P. tenuicorpa and G. brevibarba were endangered species. Length-weight relationship in the population of Z. koreanus was 3.44, and condition factor in the popuation was 0.004. The spawning season of Zacco koreanus was May and June.