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Development of PCR-RFLP Technique for Identify Several Members of Fusarium incarnatum-equiseti Species Complex and Fusarium fujikuroi Species Complex

  • Pramunadipta, Syafiqa;Widiastuti, Ani;Wibowo, Arif;Suga, Haruhisa;Priyatmojo, Achmadi
    • The Plant Pathology Journal
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    • v.38 no.3
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    • pp.254-260
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    • 2022
  • Fusarium incarnatum-equiseti species complex (FIESC) contain over 40 members. The primer pair Smibo1FM/Semi1RM on the RPB2 partial gene has been reported to be able to identify Fusarium semitectum. The F. fujikuroi species complex (FFSC) contains more than 50 members. The F. verticillioides as a member of this complex can be identified by using VER1/VER2 primer pair on the CaM partial gene. In this research, the Smibo1FM/Semi1RM can amplify F. sulawesiense, F. hainanense, F. bubalinum, and F. tanahbumbuense, members of FIESC at 424 bp. The VER1/VER2 can amplify F. verticillioides, F. andiyazi, and F. pseudocircinatum, members of FFSC at 578 bp. Polymerase chain reaction-restriction fragment length polymorphism by using the combination of three restriction enzymes EcoRV, MspI, and HpyAV can differentiate each species of FIESC used. The two restriction enzymes HpaII and NspI can distinguish each species of FFSC used. The proper identification process is required for pathogen control in the field in order to reduce crop yield loss.

Automated Ship Reporting System in the Context of e-navigation

  • An, Kwang
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.28 no.3
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    • pp.423-429
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    • 2022
  • Ship reporting systems are used to exchange information between ship and shore. To realize the digital ship reporting concept, the International Maritime Organization (IMO) recently developed revised guidelines and descriptions of Maritime Service for ship reporting systems in the context of e-navigation. To improve the existing ship reporting system, each Administration should follow the IMO guidelines for ship reporting system. The purpose of this paper is to identify follow-up measures to be taken by the Korean Government as a member State according to the recently developed IMO guidelines in the context of e-navigation, and to present the considerations for the implementation of follow-up measures in Korea. In this study, a ship reporting system in Korea was investigated to identify the considerations for the digital ship reporting system. Consequently, a digital reporting system and digital traffic clearance were proposed and considerations for ship reporting system are presented. This paper is expected to be helpful for the safe operation of ships by presenting the considerations necessary for the implementation of an automated and standardized ship reporting system.

A Preliminary Study on Development and Evaluation of Home Health Care Nurse Clinical Practice Program -Focused on Postoperative Orthopedic Patients- (가정간호사 임상실무 훈련프로그램 개발과 평가를 위한 사전 연구 -정형외과 수술 환자를 중심으로-)

  • 서영숙
    • Journal of Korean Academy of Nursing
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    • v.26 no.1
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    • pp.15-32
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    • 1996
  • The clinical practice program for home care nurses was implemented in June 1994, to help to set up a hospital-based home care system in the Kwangju City area as a collaborative work between the Department of Orthopedic Surgery at Chunnam University Hospital and Chunnam University School of Nursing. Under the developed clinical practice strategy, the eight week training was given to five licensed home care nurses who had completed Part I and II of the home health care nursing practicum from June 1994. The purpose of this descriptive evaluation study was to identify the effectiveness of the clinical practice program for home care nurses specialized in the area of patient care for people with musculoskeletal function impairment. As a method in data analysis, data triangulation was used in the five home care nurse case evaluations. The variety of data analyzed include confidence score by home care nurse self-evaluation, patient and family member satisfaction scores, and competency score by preceptor evaluation. The study findings revealed that an increase rate in nursing performance didrate necessarily coincide with an increase not in competency score and also, not with the patient /family member satisfaction scores. And an order derived from the clinical performance scores of five home care nurses corresponded to those from three measurements-competency score, patient satisfaction score, and family member satisfaction score. However, it differed from the order associated with the confidence score. Consistency derived from the three objective evaluation methods may lead to the possibility that the level of competency measured by educator can be further explained by the levels of patient/family member satisfaction. The salient finding of this study was that, in case of nurse A who have had little clinical experience in the orthopedic patient care, there was a significant increase in the level of confidence and competency in subscale of professional skill with the home care clinical practice. Therefore, the effect of the clinical practice program would be successful for nurses who have had little experience in the area of specialization. The study results suggest that there might be some time difference in the development of cognitive sense (confidence) in performance and actual clinical performance (competency). In future research, relationships between the confidence and competency score, and between the confidence score and the patient satisfaction score should to be measured in different time frame to achieve a better explanation power of the study outcome.

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Factors that Impact on Safety Activities of Personnels in Oriental Medicine Hospital of University (대학부속 한방병원 교직원의 안전활동에 영향을 미치는 요인)

  • Lee, Jung-Woo
    • Korea Journal of Hospital Management
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    • v.22 no.3
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    • pp.61-73
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    • 2017
  • Purposes : The purpose of this study is to identify factors that have effects on safety activities of hospital personnels by investigating causality between patient safety culture, job stress, safety system and safety activities of faculty and staff member who are working for oriental medicine hospital of university. Methodology : The subjects were 246 employees working in 4 oriental medicine hospitals of university in Daejeon and Chungcheongnam-do. The data were collected from January 16 to January 25, 2017 using a structured questionnaire. For data analysis, descriptive statistics, Pearson correlation coefficient, t-test, ANOVA and Duncan test with SPSS 22.0 were used. Findings : The activity score for patient safety of faculty and staff member, who were experienced at job training program after joining a company and regular training course for qualification or license, was meaningfully higher than that of group who had no job training experience. The result indicated that the higher there is level of safety culture and safety system and the lower there is work stress, the more the activity has positive effect on patient safety. The level score of awareness for safety culture of faculty and staff member in C hospital, which is facing financial crisis in business circumstances recently, showed average value of 3.29. It was significantly lower than that of the other three hospital. Also, The activity score for patient safety was markedly lower than that of the other three hospital. This result become interested in the process of linking non-financial performance and financial performance. The level score of safety activity in A hospital which obtained healthcare accreditation was remarkably higher than that of the other three hospital which didn't certify healthcare accreditation. Pratical Implications : Subjects about Q.I or patient's experience management must be included in curriculum of Oriental medicine. It is necessary to get the effect of job training program for faculty and staff member through the process of preparation for obtaining healthcare accreditation. When the hospital director is appointed, it must be considered that he/she has the ability to attach importance to analysis and management of the factors creating safety accident, and has business mindset for healthcare delivery of customer- centricity. This research showed that financial performance of hospital, which of business environment is favorable; located in metropolitan city and having large scale of hospital and quality of residence rating but low-level of safety culture and safety activity, was lower than that of general hospital situated in small and medium-sized cities. More research needs to be done for answering this result.

Genome wide association study on feed conversion ratio using imputed sequence data in chickens

  • Wang, Jiaying;Yuan, Xiaolong;Ye, Shaopan;Huang, Shuwen;He, Yingting;Zhang, Hao;Li, Jiaqi;Zhang, Xiquan;Zhang, Zhe
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.4
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    • pp.494-500
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    • 2019
  • Objective: Feed consumption contributes a large percentage for total production costs in the poultry industry. Detecting genes associated with feeding traits will be of benefit to improve our understanding of the molecular determinants for feed efficiency. The objective of this study was to identify candidate genes associated with feed conversion ratio (FCR) via genomewide association study (GWAS) using sequence data imputed from single nucleotide polymorphism (SNP) panel in a Chinese indigenous chicken population. Methods: A total of 435 Chinese indigenous chickens were phenotyped for FCR and were genotyped using a 600K SNP genotyping array. Twenty-four birds were selected for sequencing, and the 600K SNP panel data were imputed to whole sequence data with the 24 birds as the reference. The GWAS were performed with GEMMA software. Results: After quality control, 8,626,020 SNPs were used for sequence based GWAS, in which ten significant genomic regions were detected to be associated with FCR. Ten candidate genes, ubiquitin specific peptidase 44, leukotriene A4 hydrolase, ETS transcription factor, R-spondin 2, inhibitor of apoptosis protein 3, sosondowah ankyrin repeat domain family member D, calmodulin regulated spectrin associated protein family member 2, zinc finger and BTB domain containing 41, potassium sodium-activated channel subfamily T member 2, and member of RAS oncogene family were annotated. Several of them were within or near the reported FCR quantitative trait loci, and others were newly reported. Conclusion: Results from this study provide valuable prior information on chicken genomic breeding programs, and potentially improve our understanding of the molecular mechanism for feeding traits.

An Overview on Vibration or Wave Therapy in Korea (진동, 파동치료에 관한 국내 연구 동향)

  • Lee, Jae Heung;Beag, Ji You;Chang, Sung Jin;Pil, Gam Mai
    • Journal of Korean Medical Ki-Gong Academy
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    • v.20 no.1
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    • pp.15-67
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    • 2020
  • Objective : The purpose of this study is to identify the trends of vibration(or wave) therapy in Korea, to actively utilize vibration(or wave) therapy, and to help research activities of vibration therapy in Korean Medicine. Methods : The following Korean words "진동기", "진동요법", "진동운동", "진동치료", "파동요법", "파동운동", "파동치료" were searched on three specialized search sites (RISS, NAL, DBpia). Trends of vibration therapy were analyzed through the selected researches suitable for this study among these searched researches in an overview format. Results : 1. A total of 8,116 studies were searched and a total of 365 studies were finally selected 2. From 2000 to 2019, when research began to increase in earnest, there were 17.45±10.28 studies per year, and the AGR(Average Annual Growth Rate) was 11.92%. 3. In the main field of research, the 'Medicine and Pharmacy' was the largest with 147(40.16%) studies. In the Middle Field, the 'Kinesiology' was the largest with 99(27.05%) studies. In the study design, 'RCT(Randomized Controlled trial)' was the largest with 138(47.75%) studies. In the Age Group, 'Youth' was the largest with 126(48.84%) studies. 4. The average of the number of participants was 24.90±17.44. 5. The most used Intervention was the 'WBV(Whole Body Vibration)' with 177(61.25%) studies. 6. The average of Intervention Period was 5.99±4.14 weeks, while the maximum was 36 weeks. 7. The journal that published the most research papers is 'K. J. of Sports Science(체육과학연구;13)', and the society is 'Rehabilitation Engineering And Assistive Technology Society of Korea(한국재활복지공학회; 14)'. The University that published the most dissertations is 'Sahmyook University(11)'. 8. The authors who published the most studies are Ju-Hwan O(8) as the main author and Tae Kyu Kwon(18) as the co-author (including the thesis Director). In an integrated analysis of the authors and co-authors, Tae Kyu Kwon published the most numerous studies(19) Conclusions : 1. The study of vibration or wave therapy has been increasing noticeably every year. 2. The major academic Fields studying vibration or wave therapy are the 'Kinesiologic Field', 'Physical Therapy Field', and 'Biomedical Engineering Field'. 3. The most chosen method of study design on vibration or wave treatment was 'RCT', and there was no significant change in the annual presentation rate. 4. Types of vibration or wave therapy could be classified as 'LVS(Local Vibration Stimulation)', 'WBV(Whole Body Vibration)', 'MV(Micro Vibration)', 'BV(Bio Vibration)' and 'SWV(Sound Wave Vibration)', and the study on Whole Body Vibration is most active. 5. Most of the studies of vibration or wave therapy were on musculoskeletal systems, but there were very few studies on internal diseases.

Comparison of Anonymous Authentication Protocols

  • Kim, Jongseong;Kim, Kwangjo
    • Proceedings of the Korea Institutes of Information Security and Cryptology Conference
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    • 2002.11a
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    • pp.369-372
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    • 2002
  • An anonymous authentication scheme allows a user to identify himself as a member of a group of users in a secure and anonymous way. It seems to be crucial and indispensable components in English auction, electronic voting and open procurement, which are getting very popular business areas in E-commerce. First, we briefly describe the previous anonymous authentication protocols how to work and what cryptographic techniques adopted to increase performance and achieve anonymity. Second, we compare those protocols from the viewpoint of the communication and computation complexity and the specific cryptographic techniques used in their protocols.

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A Study on the Education Training Satisfaction of Employees in General Hospitals (종합병원 직원의 교육훈련 만족에 관한 연구)

  • Ahn, Sang-Yoon
    • Korea Journal of Hospital Management
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    • v.17 no.2
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    • pp.34-51
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    • 2012
  • This study is an empirical research to identify difference of education training satisfaction by demographic variables, and to investigate the influence of education training satisfaction on the member's organizational commitment in general hospitals. As a result of ANOVA, t-test based on the data of 325 employees in 7 general hospitals in Korea, education training satisfaction has a significant difference by a construct and demographic variables. Education training satisfaction has a significant difference by a construct such as education training system, education training administration and education training practice. Education training satisfaction has a significant difference by occupation, career, scholarship. Education training satisfaction has a partly significant difference by status, age. But it has not a significant difference by sex distinction. Satisfaction of education training system and education training practice has a significantly positive relationship with organizational commitment. But Satisfaction of education training administration has a partly positive relationship with organizational commitment. By these results, the advanced education training structure needs to equipped in order to elevate management performance in general hospitals.

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A systematic study of nuclear interactome of C-terminal domain small phosphatase-like 2 using inducible expression system and shotgun proteomics

  • Kang, NaNa;Koo, JaeHyung;Wang, Sen;Hur, Sun Jin;Bahk, Young Yil
    • BMB Reports
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    • v.49 no.6
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    • pp.319-324
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    • 2016
  • RNA polymerase II C-terminal domain phosphatases are newly emerging family of phosphatases that contain FCPH domain with Mg+2-binding DXDX(T/V) signature motif. Its subfamily includes small CTD phosphatases (SCPs). Recently, we identified several interacting partners of human SCP1 with appearance of dephosphorylation and O-GlcNAcylation. In this study, using an established cell line with inducible CTDSPL2 protein (a member of the new phosphatase family), proteomic screening was conducted to identify binding partners of CTDSPL2 in nuclear extract through immunoprecipitation of CTDSPL2 with its associated. This approach led to the identification of several interacting partners of CTDSPL2. This will provide a better understanding on CTDSPL2.

Food Management Pattern and Preception of Housewives in Yuanbean Area (연변지역 조선족 주부들의 식생활 관리 실태)

  • 박영선
    • Journal of the East Asian Society of Dietary Life
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    • v.11 no.6
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    • pp.430-445
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    • 2001
  • The purpose of this study was to identify the condition and perception of food management pattern in Yuanbean area. An investigation based on Previous research was administered to 200 housewives by questionnaire method in 1993 and 1995. Data were descriptively analyzed and chi-square value was calculated to compare group differences. Findings indicated that snack preference, food budget planning. menu planning. focusing factors for preparing and purchasing food materials, and family member considering for preparing meal were varied by socio-demographic factors. such as age, education. occupation. family income. religion. family type and size, years of marriage, hometown, kitchen structure, and types of fuels. Similarities and differences between groups were discussed and implications for nutrition were suggested.

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