• Journal of Microbiology and Biotechnology
• /
• v.26 no.12
• /
• pp.2076-2086
• /
• 2016

Fungal cytochrome P450 (CYP) enzymes catalyze versatile monooxygenase reactions and play a major role in fungal adaptations owing to their essential roles in the production avoid metabolites critical for pathogenesis, detoxification of xenobiotics, and exploitation avoid substrates. Although fungal CYP-dependent biotransformation for the selective oxidation avoid organic compounds in yeast system is advantageous, it often suffers from a shortage avoid intracellular NADPH. In this study, we aimed to investigate the use of bacterial glucose dehydrogenase (GDH) for the intracellular electron regeneration of fungal CYP monooxygenase in a yeast reconstituted system. The benzoate hydroxylase FoCYP53A19 and its homologous redox partner FoCPR from Fusarium oxysporum were co-expressed with the BsGDH from Bacillus subtilis in Saccharomyces cerevisiae for heterologous expression and biotransformations. We attempted to optimize several bottlenecks concerning the efficiency of fungal CYP-mediated whole-cell-biotransformation to enhance the conversion. The catalytic performance of the intracellular NADPH regeneration system facilitated the hydroxylation of benzoic acid to 4-hydroxybenzoic acid with high conversion in the resting-cell reaction. The FoCYP53A19+FoCPR+BsGDH reconstituted system produced 0.47 mM 4-hydroxybenzoic acid (94% conversion) in the resting-cell biotransformations performed in 50 mM phosphate buffer (pH 6.0) containing 0.5 mM benzoic acid and 0.25% glucose for 24 h at $30^{\circ}C$. The "coupled-enzyme" system can certainly improve the overall performance of NADPH-dependent whole-cell biotransformations in a yeast system.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.3 no.4
• /
• pp.22-32
• /
• 2000

Development of index for evaluation of environmental nature and its application for the purpose of construction of industrial complex with environmentally friendly. Industrial complex comes to be recognized as a disgusting zone according to the increasing environment consciousness of residents. And the objection about the exploitation of geographically unwanted land site is increased during development process and it is possible to cause Nimby phenomenon. Therefore, ecology and environment-oriented strategies tends to be adopted for industrial complex development. Furthermore, the maximization of profit mainly directed by enterprises as of today cannot guarantee its future and the efforts of adopting environmental constancy and harmony as a new objective are made progressively. For the development of city and region, in particular such as residence complex, farming and special city, the discussion of environmental consistency, new paradigm with environmentally friendly is briskly under progress, but the relevant discussion for the development of industrial estate is not done so much. Just only few studies are realized from the viewpoint of industrial ecology but its level remains in the understanding of the concepts and presentation of development direction. This study is to prepare for the foundation of the construction of industrial estate with environmentally friendly under the current condition of Korea against coming future environment era and to try to develop of evaluation range and index which are the basis of evaluation of environment. I applied the above evaluation index to the residents of actual industrial estate to understand their consciousness of decreased environment. And I obtained 7 evaluation ranges and 37 evaluation indexes for the relevant residents. Among them, I get the value less than the average in 19 index items. And they represent very sensible reaction about environment pollution including decrease of waste materials. This study has several limits but is meaningful in that the development of evaluation index for environmental nature in industrial complex is done, and that the basic discussion of the plan and design for environmentally friendly industrial estate is made under the above evaluation and that 37 evaluation items are applied to the reality.

• Journal of Information Technology Applications and Management
• /
• v.14 no.2
• /
• pp.27-47
• /
• 2007

In an effort to apply IT to practices of learning, universities are trying to implement e-Learning systems and expanding the extent of their usage. Nowadays, e-Learning systems are not only used for conducting web-based distance learning, but also used for supporting traditional classes education by encouraging communication and information sharing between instructors and learners or among the learners. There is relatively lack of studies on the exploitation of e-Learning systems in the traditional classes, in comparison with a distance education. Specifically, howe-Learning systems could support the traditional class and/or which benefits e-Learning systems could offer in the classes are among the important issues. In this study, we suggest that e-Learning systems would be the rich media to communicate and exchange information among people who participate in a class. We derive key variables like media richness and media experience from Media Richness Theory and from Channel Expansion Theory. Moreover, Media usefulness and Satisfaction of a learner with e-Learning system is drawn from the literature on IS success. We examine the effects of perceived media richness, media usefulness, and media experience on leaner's satisfaction with e-Learning systems. In addition, we also investigate learner's media usefulness perception which is positively related to media richness and media experience. Finally, learner's experience with e-Learning systems affects perceived media richness. Based on the results of an empirical test. we first suggest that perceived media richness with e-Learning systems contributes to increase media usefulness and satisfaction of a learner. Second, media experience is an important predictor of media richness and media usefulness perception. Consequently, the result can support Channel Expansion Theory. Finally, media usefulness perception affects learner's satisfaction with e-Learning systems.

• Animal cells and systems
• /
• v.13 no.4
• /
• pp.419-427
• /
• 2009

The Minke whale, Balaenoptera acutorostrata, is the smallest baleen whale in the suborder Mysticeti. Because this species inhabits coastal areas, it became a main target species of coastal small-type whaling in the North Atlantic and the Northwest Pacific Oceans, and the species' population size dramatically decreased because of over-exploitation. As a result, the International Whaling Commission declared a global moratorium on whaling and launched the development of a management procedure for protecting the whales. Morphological studies, whaling history analysis, and genetic studies conducted mainly by Japanese scientists showed the existence of one unique "E" stock that inhabits the waters around the Korean peninsula and mixes with the "O" stock in the southern part of the Sea of Okhotsk. We used the mitochondrial DNA control region polymorphism of 348 Minke whales bycaught or stranded in Korean waters from 30 October 1998 to 25 June 2005 to assess the whale population structure by year. The frequency of the 10 major haplotypes from the 40 identified haplotypes was not significantly different among groups, suggesting that a subpopulation was not present. A comparison of the genetic distances calculated with Tamura-Nei's method showed that the distances between groups were lower than those within groups, which suggests that there was no genetic difference in the Minke whale populations. The Fst comparison between groups and the phylogenetic tree constructed using the unweighted pair group method with arithmetic mean (UPGMA) and Neighbor Joining (NJ) method also detected no obvious sub-stock structure.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2000.10a
• /
• pp.114-115
• /
• 2000

Aralia elata is found in mountain areas all over Korean peninsula. Aralia elata is the scientific name for Japanese angelica tree. The tree belongs to the family Araliaceae, commonly known as ginseng family. Bud sprouts from apical shoot tip of the plants are rich in flavor and thus mainly used for both folk medicine and vegetable. The stalks with apical buds are gathered in the early spring and planted in sandy soil or water in the greenhouse. The sprouting buds are then collected and sold as fresh vegetable. Although the plants have been used for food, they have been cultivated in a very small scale. In spring, local farmers just go around mountain areas to search the trees and gather the stalks as much as they get and sell them to the market. No conservation efforts have been made to stop the exploitation or to save the dwindling population. We tried to provide local farmers with the plants that may be used as an alternative to stalks from wild populations. This will bel! p conserve the wild populations. However, it is hard to propagate them either by conventional cuttings or by seed germination in a short period of time. Mass propagation using tissue culture systems have shown a great promise with several woody plants. Recently we developed a mass propagation technique via somatic embryogenesis system using mature and/or juvenile explants for Aralia elata. Several factors affecting somatic embryogenesis system including SE(somatic embryo) induction, embryogenic callus proliferation, SE germination, plant regeneration and transplanting to field frill be presented. And some problems arising for the somatic embryogenesis system will be also discussed.

• Molecules and Cells
• /
• v.19 no.3
• /
• pp.398-401
• /
• 2005

The bacterial enzyme UDP-N-acetylglucosamine enolpyruvyl transferase catalyzes the first committed step of peptidoglycan biosynthesis, i.e., transfer of enolpyruvate from phosphoenolpyruvate to UDP-N-acetyl-glucosamine. We have overexpressed the enzyme from Haemophilus influenzae in Escherichia coli and crystallized it in the apo-form, as well as in a complex with UDP-N-acetylglucosamine and fosfomycin using ammonium sulfate as the precipitant. X-ray diffraction data from a crystal of the apo-form were collected to $2.8{\AA}$ resolution at 293 K. The crystal quality was improved by co-crystallization with UDP-N-acetylglucosamine and fosfomycin. X-ray data to $2.2{\AA}$ have been collected at 100 K from a flash-frozen crystal of the complex. The complex crystals belong to the orthorhombic space group I222 (or $I2_12_12_1$) with unit-cell parameters of a = 63.7, b = 124.5, and $c=126.3{\AA}$. Assuming a monomer of the recombinant enzyme in the crystallographic asymmetric unit, the calculated Matthews parameter ($V_M$) is $2.71{\AA}^3Da^{-1}$ and solvent content is 54.6%.

• Journal of Construction Engineering and Project Management
• /
• v.8 no.3
• /
• pp.1-23
• /
• 2018

Since the protection the of human being from natural disaster and atmospheric factors have become an essential requirement, some attempts have been taken place to provide shelter and create a safe environment to a more comfortable life with welfare. For this purpose, using existing resources in nature and exploiting them in a different manner have been taken into account. Initially, the performed exploitations for construction had the least damage to the environment, but over time and as a result of population growth, aggressive exploitation of nature has led to destroying effects on the environment and resulted in consequences such as pollution and environmental destruction. Thus, the construction industry has been identified as the top pollutant factors of the environment. Among various construction factors, the building materials used in this industry are considered as the most important effective factors on the environment, as they have direct influences on the environment from the beginning of construction of the final steps. This research focuses on the review of the most of the existing green materials definitions and various approaches towards using eco-efficient construction materials. It presents and discusses possible ways of reducing the destructive effects on the environment by selecting and using green materials, review current literature and highlight the necessity of applying such materials in future constructions in all communities. This paper provides a base for this purpose that sustainable development communities and environment is realized by elimination of environmental pollution and approaching the criteria of green building by using sustainable materials.

• Journal of Digital Convergence
• /
• v.11 no.7
• /
• pp.173-184
• /
• 2013

Quantum Information Processing and Communication, based on the physical laws of Quantum mechanics, exploits fundamentally new modes of computation and communication and holds the promise of immense computing power beyond the capabilities of any classical computer. In Quantum Information Processing, replacing bits with qubits, one makes two-state quantum systems that do not possess in general the definite values of 0 or 1 of classical bits, but rather are in a so-called. "coherent superposition", of the two. Full exploitation of this additional freedom implies that new processing devices need to be designed and implemented, and that a large scale quantum computer can in principle be built. New discoveries will enable a range of exciting new possibilities including: greatly improved sensors with potential impact for mineral exploration and improved medical imaging and a revolutionary new computational paradigm that will likely lead to the creation of computing devices capable of efficiently solving problems that cannot be solved on a classical computer. In short, Quantum computing is an economy game changer, with a potential of disrupting entire industries and creating new ones.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2000.10b
• /
• pp.16-17
• /
• 2000

Aralia elata is found in mountain areas all over Korean peninsula. Aralia elata is the scientific name for Japanese angelica tree. The tree belongs to the family Araliaceae, commonly known as ginseng family. Bud sprouts from apical shoot tip of the plants are rich in flavor and thus mainly used for both folk medicine and vegetable. The stalks with apical buds are gathered in the early spring and planted in sandy soil or water in the greenhouse. The sprouting buds are then collected and sold as fresh vegetable. Although the plants have been used for food, they have been cultivated in a very small scale. In spring, local farmers just go around mountain areas to search the trees and gather the stalks as much as they get and sell them to the market. No conservation efforts have been made to stop the exploitation or to save the dwindling population. We tried to provide local farmers with the plants that may be used as an alternative to stalks from wild populations. This will hel! p conserve the wild populations. However, it is hard to propagate them either by conventional cuttings or by seed germination in a short period of time. Mass propagation using tissue culture systems have shown a great promise with several woody plants. Recently we developed a mass propagation technique via somatic embryogenesis system using mature and/ or juvenile explants for Aralia elata. Several factors affecting somatic embryogenesis system including SE(somatic embryo) induction, embryogenic callus proliferation, SE germination, plant regeneration and transplanting to field will be presented. And some problems arising for the somatic embryogenesis system will be also discussed.lso discussed.

• Journal of Korean Society of Archives and Records Management
• /
• v.11 no.1
• /
• pp.253-271
• /
• 2011

The modern age is such an era of time and space compressed. The central axis having compressed the time of the modern Busan city was the very trains and trams. The development of the means of modern transportation, such as trans and trams, came to change the time-space characteristics of cities in the modern age. Through trains and trams regarded as symbols of modern cities, this study attempted to find the localities of Busan as a modern city. The development of the modern Busan city was actually based on modernity and colonial exploitation. Documents of localities provide data to read humans' local-related experiences. 'Documenting localities' related to the means of modern transportation is a work process to read the localities of Busan in the modern age. Through this process, it is possible to understand how means of transportation can be correlated with a local community, local residents' daily lives and spatial change. Overall, this study is such a small attempt for this concern.