• Korean Journal of Food Science and Technology
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• v.32 no.6
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• pp.1221-1226
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• 2000

Specific antibodies were produced to develope the enzyme-linked immunosorbent assay for analysis of soy proteins and the properties of the antibodies were compared. Isolate soy protein(ISP), and ISP heated with SDS and urea (ISP(SU)), acidic subunits(AS) of 11S globulin were immunized to produce polyclonal antibodies. By using competitive indirect ELISA(ciELISA), the reactivities of the antibodies toward soy proteins treated with different methods were investigated and shown as $IC_{50}$. $IC_{50}'s$ of anti-ISP antibodies to ISP, ISP(SU), ISP treated with 2-ME(ISP(ME)), and crude 11S were 20, 30, 36, and $1000\;{\mu}g/mL$, respectively. And the values of anti-ISP(SU) antibodies to the same antigens were 100, 5, 4, and $220\;{\mu}g/mL$ and those of anti-AS antibodies were 20, 2, 2.5, and $200\;{\mu}g/mL$, respectively. Therefore, anti-AS antibodies showed the highest reactivities toward soy proteins among the produced antibodies as determined by ciELISA.

• Nuclear Engineering and Technology
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• v.44 no.6
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• pp.561-586
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• 2012

This paper describes a summary of final prediction results by system-scale safety analysis codes during the OECD/NEA/CSNI ISP-50 exercise, targeting a 50% Direct Vessel Injection (DVI) line break integral effect test performed with the Advanced Thermal-Hydraulic Test Loop for Accident Simulation (ATLAS). This ISP-50 exercise has been performed in two consecutive phases: "blind" and "open" phases. Quantitative comparisons were performed using the Fast Fourier Transform Based Method (FFTBM) to compare the overall accuracy of the collected calculations. Great user effects resulting from the combination of the possible reasons were found in the blind phase, confirming that user effect is still one of the major issues in connection with the system thermal-hydraulic code application. Open calculations showed better prediction accuracy than the blind calculations in terms of average amplitude (AA) value. A total of nineteen organizations from eleven countries participated in this ISP-50 program and eight leading thermal-hydraulic system analysis codes were used: APROS, ATHLET, CATHARE, KORSAR, MARS-KS, RELAP5/MOD3.3, TECH-M-97, and TRACE.

• Korean Journal of Food Science and Technology
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• v.35 no.2
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• pp.182-187
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• 2003

Quality attributes of reconstructed pork patties with ISP were evaluated. Reconstructed pork patties with 30% meat plus ISP and 50% meat plus had significantly less cooking loss and dimensional changes than control. Sensory evaluation revealed patties with 30 or 50% meat had higher hardness and juiciness than control, patties with ISP, and patties with direct addition of ISP. Objective elasticities of patties with 30 or 50 % meat were high, whereas patties without ISP had higher values of hardness, gumminess, and chewiness. Color of patties with 30 or 50% meat were different from that of control. These result show addition of ISP to meat emulsion for pork patties markedly improved cooking loss, dimensional changes, hardness, and juiciness. When pork patties and cutlets prepared according to meat (30%) formula were frozen, cooking loss was significantly higher in slow-frozen patties, but freezing rate did not affect dimensional changes of patties and cutlets. Slow-frozen patties had higher hardness, but other textural properties were affected by the freezing rate. Quality of pork cutlets was not significantly changed by the freezing rate.

• Nuclear Engineering and Technology
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• v.44 no.7
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• pp.719-726
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• 2012

The object of this work is the validation and assessment of the TRACE v5.0 code using the scaled test ATLAS1 facility in the context of a DVI2 line break. In particular, the experiment selected models the 50%, 6-inch break of a DVI line. The same experiment was also adopted as a reference test in the ISP-503. The ISP-50 was proposed to, and accepted by, the OECD/NEA/CSNI due to its technical importance in the development of a best-estimate of safety analysis methodology for DVI line break accidents. In particular, the behavior of the two-phase flow in the upper annulus downcomer was expected to be complicated. What resulted was the need for relevant models to be implemented into safety analysis codes, in order to predict these thermal hydraulic phenomena correctly.

• Nuclear Engineering and Technology
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• v.44 no.6
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• pp.587-596
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• 2012

The present paper deals with the participation of the University of Pisa in the last International Standard Problem (ISP) focused on system thermal hydraulic, which was led by the Korean Atomic Energy Research Institution (KAERI). The selected test was a Direct Vessel Injection (DVI) line break carried out at the ATLAS facility. University of Pisa participated, together with other eighteen institutions, in both blind and open phase of the analytical exercise pursuing its methodology for developing and qualifying a nodalization. Qualitative and quantitative analysis of the code results have been performed for both ISP-50 phases, the latter adopting the Fast Fourier Transfer Based Method (FFTBM). The experiment has been characterized by three-dimensional behavior in downcomer and core region. Even though an attempt to reproduce these phenomena, by developing a fictitious three-dimensional nodalization has been realized, the obtained results were generally acceptable but not fully satisfactory in replicating 3D behavior.

• Journal of the Korean Society of Food Science and Nutrition
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• v.12 no.2
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• pp.73-83
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• 1983

This experiment was conducted to study effects of different sources of protein, and of the different combination ratios of the two protein supplemented or unsupplemented with methionine on the growth and body metabolism of the growing male rats. Casein and isolated soyprotein (ISP) were combined at different ratios of 100:0, 80:20, 50:50, 20:80 and 0:100 to supply the constant levels of dietary energy and protein of 3,600kcal/kg and 20%, respectively. Each combination was supplemented with either 0 or 0.3% methionine. Results obtained in these experiments were summarized as follows: The body weight and weight gain in the ISP diet were the lowest, but were increased with the increase of ratios of casein to ISP. The body weight gain in the ISP diet supplemented with methionine was similar to that in the combination diets of casein and ISP. The combination diets of casein and ISP supplemented with methionine had the higher weight gain than the casein diet. The food intake in the casein diet was the highest, and was increased with the increase of ratios of casein to ISP, and was lowered with methionine supplementation. The food intake in the ISP diet was the lowest and was not increased with methionine supplementation to the ISP diet. The food efficiency ratio of the combination diets of casein and ISP was improved compared to those of either the casein or the ISP diets. The food efficiency ratio was improved in the diet of either casein of ISP supplemented with methionine, but was not improved in the combination diets of casein and ISP with methionine supplementation. The gross energy intake had direct relation with the food intake of rats and the relation was increased either in the casein diet of with the increase of the ratios of casein to ISP. The energy efficiency and protein efficiency ratios were improved in the diet of either casein of ISP by the supplementation of methionine, and the effect of methionine supplementation of the energy efficiency and protein efficiency atio was increased with the increase of the ratios of ISP to casein. The nitrogen intake and urinary nitrogen excretion of the casein diet were the highest and those of the ISP diet were the lowest. They were increased with the increase of ratios of casein to ISP. The nitrogen balance and retention were not significantly among the treatments. The gross energy intake and fecal and urinary energy were the highest in the casein diet but the digestible and metabolizable energy and the digestibility and metabolizability of energy were not significantly different among the treatments. The body fat content of rats was increased by supplementation of methionine but the body protein content was decreased (r=-0.65, p<0.01). The body fat content of rats was negatively correlated with body moisture content (r=-0.83, p<0.01). The liver weight was highly correlated with the carcass weight (r=0.79, p<0.01), and was increased by the methionine supplementation and by the increase of the ratios of casein to ISP.

• Nuclear Engineering and Technology
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• v.44 no.7
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• pp.709-718
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• 2012

The pressurized water reactor APR1400 adopts DVI (Direct Vessel Injection) for the emergency cooling water in the upper downcomer annulus. The International Standard Problem number 50 (ISP-50) was launched with the aim to investigate thermal hydraulic phenomena during a 50% DVI line break scenario with best estimate codes making use of the experimental data available from the ATLAS facility located at KAERI. The present work describes the calculation results obtained for the ISP-50 using the RELAP5/MOD3.3 system code. The work aims at validation and assessment of the code to reproduce the observed phenomena and investigate about its limitations to predict complicated mixing phenomena between the subcooled emergency cooling water and the two-phase flow in the downcomer. The obtained results show that the overall trends of the main test variables are well reproduced by the calculations. In particular, the pressure in the primary system show excellent agreement with the experiment. The loop seal clearance phenomenon was observed in the calculation and it was found to have an important influence on the transient progression. Moreover, the collapsed water levels in the core are accurately reproduced in the simulations. However, the drop in the downcomer level before the activation of the DVI from safety injection tanks was underestimated due to multi-dimensional phenomena in the downcomer that are not properly captured by one-dimensional simulations.

• Journal of Pharmaceutical Investigation
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• v.23 no.3
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• pp.41-50
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• 1993

The iontophoretic delivery across rat skin of quaternary ammonium salts (isopropamide: ISP, pyridostigmine: PS), which are positively charged over a wide pH range, was measured ill vitro. The study showed that: (a) iontophoresis significantly enhanced delivery of ISP and PS compared to respective passive transport; (b) delivery of ISP and PS was directly proportional to the applied continuous direct current density over the range of $0-0.69\;mA/cm^2;$ (c) delivery of ISP and PS was also proportional to the drug concentration in the donor compartment over the range of $0-2{\time}l0^{-2}M:$ (d) sodium ion in the donor compartment inhibited the drug transport possibly due to decreasing the electric transference number of the drug; (e) delivery of ISP and PS increased as the pH of the donor solution increased over the pH range 2-7 suggesting permselective nature of the epidermis, and inhibition of the transference number of the drugs by hydronium ion; (f) some organic anions such as taurodeoxycholate, salicylate and benzoate which form lipophilic ion-pair complexes with ISP inhibited the delivery of ISP. The degree of inhibition by the organic anions was linearly proportional to the extraction coefficient $(K_e)$ of ISP from the partition system with each counteranion between phosphate buffer (pH 7.4) and n-octanol. For PS, however, taurodeoxycholate, but not salicylate and benzoate inhibited the iontophoretic delivery. It suggests that not only sodium ion and hydronium ion but also the counteranions which form lipophilic ion-pairs with quaternary ammonium drugs are not favorable components in formulating the donor solution of the drugs to achieve an effective iontophoretic delivery.

• Journal of the Institute of Electronics and Information Engineers
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• v.50 no.2
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• pp.60-69
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• 2013

Highlighted by recent security breaches including Google, Western Energy Company, and the Stuxnet infiltration of Iranian nuclear sites, Cyber warfare attacks pose a threat to national and global security. In particular, targeted attacks such as APT exploiting a high degree of stealthiness over a long period, has extended their victims from PCs and enterprise servers to government organizations and critical national infrastructure whereas the existing security measures exhibited limited capabilities in detecting and countermeasuring them. As a solution to fight against such attacks, we designed and implemented a security monitoring system, which shares security information and helps cooperative countermeasure. The proposed security monitoring system collects security event logs from heterogeneous security devices, analyses them, and visualizes the security status using 3D technology. The capability of the proposed system was evaluated and demonstrated throughly by deploying it under real network in a ISP for a week.

• BMB Reports
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• v.40 no.6
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• pp.911-920
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• 2007

Tuberculosis, caused by Mycobacterium tuberculosis, continues to be one of the leading infectious diseases to humans. It is urgent to discover novel drug targets for the development of antitubercular agents. The 2-C-methyl-Derythritol-4-phosphate (MEP) pathway for isoprenoid biosynthesis has been considered as an attractive target for the discovery of novel antibiotics for its essentiality in bacteria and absence in mammals. MEP cytidyltransferase (IspD), the third-step enzyme of the pathway, catalyzes MEP and CTP to form 4-diphosphocytidyl-2-C-methylerythritol (CDP-ME) and PPi. In the work, ispD gene from M. tuberculosis H37Rv (MtIspD) was cloned and expressed. With N-terminal fusion of a histidine-tagged sequence, MtIspD could be purified to homogeneity by one-step nickel affinity chromatography. MtIspD exists as a homodimer with an apparent molecular mass of 52 kDa. Enzyme property analysis revealed that MtIspD has high specificity for pyrimidine bases and narrow divalent cation requirements, with maximal activity found in the presence of CTP and $Mg^{2+}$. The turnover number of MtIspD is $3.4 s^{-1}$. The Km for MEP and CTP are 43 and $92{\mu}M$, respectively. Furthermore, MtIspD shows thermal instable above $50^{\circ}C$. Circular dichroism spectra revealed that the alteration of tertiary conformation is closely related with sharp loss of enzyme activity at higher temperature. This study is expected to help better understand the features of IspD and provide useful information for the development of novel antibiotics to treat M. tuberculosis.