• Asian-Australasian Journal of Animal Sciences
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• 제32권8호
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• pp.1095-1103
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• 2019

Objective: Among stress responses, the unfolded protein response (UPR) is a well-known mechanism related to endoplasmic reticulum (ER) stress. ER stress is induced by a variety of external and environmental factors such as starvation, ischemia, hypoxia, oxidative stress, and heat stress. Inositol requiring enzyme $1{\alpha}$ ($IRE1{\alpha}$)-X-box protein 1 (XBP1) is the most conserved pathway involved in the UPR and is the main component that mediates $IRE1{\alpha}$ signalling to downstream ER-associated degradation (ERAD)- or UPR-related genes. XBP1 is a transcription factor synthesised via a novel mechanism called 'frame switch splicing', and this process has not yet been studied in the horse XBP1 gene. Therefore, the aim of this study was to confirm the frame switch splicing of horse XBP1 and characterise its dynamics using Thoroughbred muscle cells exposed to heat stress. Methods: Primary horse muscle cells were used to investigate heat stress-induced frame switch splicing of horse XBP1. Frame switch splicing was confirmed by sequencing analysis. XBP1 amino acid sequences and promoter sequences of various species were aligned to confirm the sequence homology and to find conserved cis-acting elements, respectively. The expression of the potential XBP1 downstream genes were analysed by quantitative real-time polymerase chain reaction. Results: We confirmed that splicing of horse XBP1 mRNA was affected by the duration of thermal stress. Twenty-six nucleotides in the mRNA of XBP1 were deleted after heat stress. The protein sequence and the cis-regulatory elements on the promoter of horse XBP1 are highly conserved among the mammals. Induction of putative downstream genes of horse XBP1 was dependent on the duration of heat stress. We confirmed that both the mechanisms of XBP1 frame switch splicing and various binding elements found in downstream gene promoters are highly evolutionarily conserved. Conclusion: The frame switch splicing of horse XBP1 and its dynamics were highly conserved among species. These results facilitate studies of ER-stress in horse.

• 대한치과교정학회지
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• 제24권1호
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• pp.87-94
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• 1994

The purpose of this study was to evaluate the torque effect of othodontic wires. Ten types of orthodontic wires (five types of materials, two types of cross-sectional dimensions) were selected. Each group of ire type was constituted with five specimens. These specimens were tested on the universal testing machine(Instron) with specially-designed jig. The torque-twist curve of each wire was obtained and the results were analyzed statistically. The results were as follows. 1. 0.017'$\times$ 0.025' wire showed more torque effect than 0.016'$\times$ 0.022' wire at the same twist. 2.Torque effect was the greatest in stainless steel and the least in Nitinol. 3.The maximum amount of torque was the greatest in heat-treated Blue Elgiloy and the least in Nitinol.

• 대한의생명과학회지
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• 제14권1호
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• pp.59-62
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• 2008

This experiment was performed to study the effect of TSH (thyroid-stimulating hormone) on the expression of endoplasmic reticulum (ER) chaperones in the rat thyrocytes FRTL-5 cells. Although the expressions of ER membrane kinases (ATF6, IRE1 and PERK) were specially enhanced under absence of TSH, no remarkable up- or down regulations of ER chaperones (BiP, CHOP and Calnexin) were detected by TSH. We firstly report here that TSH by dose up-regulated expression of ER membrane kinases in FRTL-5 culture thyrocytes.

• 대한의생명과학회지
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• 제16권1호
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• pp.65-67
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• 2010

The endoplasmic reticulum (ER) is a multifunctional intercellular organelle in which several posttranslational modification steps occurred such as protein folding, lipid biosynthesis, calcium storage and release. Perturbations that disrupt ER homeostasis lead to the misfolding of proteins in the ER lumen and up-regulation of ER signaling pathway called the unfolded protein response (UPR). Here, we have demonstrated that ageing changes the expression of ER chaperone and associated ER membrane kinases of IRE1, ATF6 and PERK.

• 한국군사과학기술학회지
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• 제9권3호
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• pp.101-108
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• 2006

This paper describes the design, fabrication, and measured results of a W-band Cassegrain antenna suitable for the target detecting fuze sensor. The Cassegrain antenna is designed using MATLAB and MWS of CST. We use the multi-mode horn antenna as a feeder. The measurement results are as follows: The gain is about 41dB; SLL is 17.7dB; 3dB beamwidth is about $1.51^{\circ}$ in E-plane and $1.45^{\circ}$ in H-plane. The magnitude of leakage signals is about 43.5mVpp when the fabricated antenna and the transceiver of the fuze sensor ire combined. As a result, the designed W-band Cassegrain antenna could be quite applicable to the target detecting fuze sensor.

• 생명과학회지
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• 제25권4호
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• pp.473-480
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• 2015

진핵세포의 소포체는 분비를 담당하는 첫 번째 기관이다. 대부분의 분비단백질과 막 형성단백질은 소포체에서 세포질/핵으로 전달되는 신호전달에 의한 번역후수식에 의해서 소포체를 통해서 분비된다. 그 결과 완전하게 접 힘이 일어난 단백질만 세포 밖으로 분비된다. 소포체내에서 완전하게 접힘이 일어나지 않아 축적된 단백질은 세 포내스트레스(소포체스트레스)가 되어 unfolded protein response (UPR)시스템을 작동시킨다. UPR을 작동시키는 3종류의 소포체막단백질은 inositol requiring 1 (IRE1), PKR-like ER kinase (PERK), activating transcription factor 6 (ATF6)이 존재한다. 최근에 새로운 종류의 ATF6이 동정되었다. 이들은(Luman, OASIS, BBF2H7, CREBH, CREB4) 공통적으로 소포체막관통영역, 전사활성영역, bZIP영역을 가지며 특이조직과 세포내기관에서 기능을 가 진다. 현재로서는 OASIS family의 정확한 분자기전 설명은 어렵지만, 본 리뷰에서 이들 분자신호를 포괄적으로 소개할 것이다

• The Korean Journal of Ecology
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• 제26권5호
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• pp.251-254
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• 2003

To control the spread of surface fire in the rangelands and to check the entry of fire into forest systems from grassland patches, 26 evergreen / succulent species were selected and among them 3 shrubby species such as Berberis tintoria Lesch, Elaeagnus kologa Schlecht and Rhodomyrtus tomentosa Wt. were screened for vegetational fire breaks on the basis of leaf moisture content and some other ecological characters. The fire trail experiments indicate that crossing of fire was checked by the fire belt size of 5 ${\times}$ 1.5m for all the three species. However, due to higher fire retardant capacity, the Ire belt of R. tomentosa was effective in comparison to other two species.

• 한국안전학회지
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• 제13권1호
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• pp.40-46
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• 1998

This paper is presented that theoretical optimization design method in order to consider mass reduction for the structural safety In this paper, it described methods for reducing vibration in structural safety by the determination of the optimum sizes and locations of tunning masses through formal mathematical optimization techniques. The optimization procedure which employs the tunning masses and corresponding locations is developed. Design variables are systematically changed to achieve low values of shear without a large mass penalty. Three optimization methods ire developed and tested. The first is based on minimizing the modal shaping parameter which indirectly reduce the modal shear amplitudes corresponding to each harmonic of airload. The second method reduces these amplitudes directly and the third method reduces the shear as a function of time during a revolution of the blade. The first method works well for reducing the shear for one mode responding to a single harmonic of the airload but has been found in some bases to be ineffective for more than one mode.

• 한국표면공학회지
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• 제36권5호
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• pp.398-405
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• 2003

The stainless steel wire requires good corrosion resistance and mechanical properties, such as drawing ability, combined with a high resistance to corrosion. For increasing drawing ability of stainless steel, Ni coating methods have been used in this study. However, there is no information on the electrochemical corrosion behavior of drawed wires after Ni coating. To investigate corrosion resistance and mechanical property of drawed wire, the characteristics of Ni coated wires have been determined by tensile strength tester, hardness tester, field emission scanning microscope, energy dispersive x-ray analysis and potentiodynamic method in 0.1 M HCl. The drawed stainless steel wires showed the strain-induced martensitic structure, whereas non-drawed stainless steel wire showed annealing twin in the matrix of austenitic structure. The hardness and tensile strength of drawed stainless steel wire were higer than that of non-drawed stainless steel wire. Electrochemical measurements showed that, in the case of drawed stainless steel o ire after Ni coating, the corrosion resistance and pitting potential increased compared with non-coated and drawed stainless steel wire due to decrease in the surface roughness.

• 한국의류학회지
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• 제15권4호
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• pp.405-416
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• 1991

This paper examined the risks perceived by consumers in apparel buying situation by 1) measuring the contents and perception level of risk, 2) categorizing each risk into meaningful factors (risk types), 3) analyzing the relationships between risk types and consumers' demo-graphic variables. 224 respondents deliberately selected to include each level of S demographic variables were contacted with 37-item question3.ire. Factor analysis showed that 32-item perceived risk could be categorized into 6 risk types: psychological. social, economic, time/convenience loss, fashionability loss, performance risk. Psychological risk were perceived highest in terms of perception level while social and performance risk showed relatively low perception level. 4 of 5 demographic variables including sex, education level, income, occupa-tion showed partial relationship with each risk type after ANOVA and Duncan test. Sex had the greatest influence on risk perception level and each age level (20's, 30's, 40's) showed different risk structure.