• Korean Journal of Microbiology
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• v.49 no.4
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• pp.328-335
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• 2013

As a result of conducting a cultural experiment of tomato using chicken feather protein hydrolysate (CPH) which was mass produced by keratin protein degrading bacterium Chryseobacterium sp. FBF-7 (KACC 91463P), we found that the stem and the root of tomato showed significant improvement in growth. For the purpose of phylogenic interpretation, a comparison was drawn between the effect of CPH, a treated CPH and untreated, on the changes of bacterial populations by 454 pyrosequencing based on 16S rRNA gene sequences. Tomato rhizosphere soil untreated with CPH (NCPH) showed 6.54 Shannon index from 3,281 sequence reads, and the rhizosphere soil treated with CPH (TCPH) showed 6.33 Shannon index from 2,167 sequence reads, displaying that it does not affect the diversity. Bacterial populations were composed of 19 phyla in the rhizosphere soil, and the phylum Proteobacteria occupied 40% of total bacterial populations. Bradyrhizobium, Agromonas, Nitrobacter, and Afipia (BANA group) which belong to Bradyrhizobiaceae were abundant and commonly detected in both the treated and untreated soils, suggesting the dominance of bacterial group in rhizosphere soil. The results obtained showed that CPH treatment does not affect the indigenous bacterial populations present in the rhizosphere soil.

• Mycobiology
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• v.45 no.3
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• pp.160-171
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• 2017

Larvae of Bradysia agrestis, an insect vector that transports plant pathogens, were sampled from geographically isolated regions in Korea to identify their cutaneous fungal and bacterial flora. Sampled areas were chosen within the distribution range of B. agrestis; each site was more than 91 km apart to ensure geographical segregation. We isolated 76 microbial (fungi and bacteria) strains (site 1, 29; site 2, 29; site 3, 18 strains) that were identified on the basis of morphological differences. Species identification was molecularly confirmed by determination of universal fungal internal transcribed spacer and bacterial 16S rRNA gene sequences in comparison to sequences in the EzTaxon database and the NCBI GenBank database, and their phylogenetic relationships were determined. The fungal isolates belonged to 2 phyla, 5 classes, and 7 genera; bacterial species belonged to 23 genera and 32 species. Microbial diversity differed significantly among the geographical groups with respect to Margalef's richness (3.9, 3.6, and 4.5), Menhinick's index (2.65, 2.46, and 3.30), Simpson's index (0.06, 0.12, and 0.01), and Shannon's index (2.50, 2.17, and 2.58). Although the microbial genera distribution or diversity values clearly varied among geographical groups, common genera were identified in all groups, including the fungal genus Cladosporium, and the bacterial genera Bacillus and Rhodococcus. According to classic principles of co-evolutionary relationship, these genera might have a closer association with their host insect vector B. agrestis than other genera identified. Some cutaneous bacterial genera (e.g., Pseudomonas) displaying weak interdependency with insect vectors may be hazardous to agricultural environments via mechanical transmission via B. agrestis. This study provides comprehensive information regarding the cutaneous microflora of B. agrestis, which can help in the control of such pests for crop management.

• Journal of Microbiology and Biotechnology
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• v.30 no.6
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• pp.804-810
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• 2020

The profitability of the dairy and beef industries is largely affected by the actually achieved reproductive efficiency. Although a large proportion of cows worldwide are bred by artificial insemination (AI) services, many potential factors affecting the outcome of pregnancy by AI remain to be addressed. In the present study, we investigated the vaginal microbiota by high-throughput sequencing of 16S rRNA gene and analyzed their association with differential pregnancy outcomes (i.e., pregnant vs. nonpregnant) of multiple AI services in dairy cows. Sequencing of the V3-V4 region totally produced 512,046 high-quality sequences that were computationally clustered into 2,584 operational taxonomic units (OTUs). All OTUs were taxonomically assigned to 10 bacterial phyla. There were statistically significant differences among the three AI service times (T1, T2 and T3) with respect to the Shannon index and number of observed OTUs (p < 0.05). Bray-Curtis distance-based PCoA analysis also revealed that T2 group could be significantly distinguished from T1 and T3. However, no significant difference between the pregnant and nonpregnant cows was found in confidence regarding both alpha diversity and beta diversity. These results could help us better understand the possible influence of vaginal microbial community on pregnancy outcomes of AI service in cows.

• Journal of Nutrition and Health
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• v.57 no.1
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• pp.75-87
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• 2024

Purpose: Dietary habits are strongly related to the symptoms of people with irritable bowel syndrome (IBS). Therefore, personalized nutrition management can help reduce symptoms and improve the quality of life of people with IBS. This study assessed the effectiveness of a personalized web-based nutrition management based on the types of food that trigger IBS symptoms. Methods: Sixty Korean adults with IBS according to Rome IV criteria in their 20s and 30s were enrolled in this study. The data from the final 49 patients who completed a three-month personalized nutrition intervention were analyzed. The general information, anthropometry, dietary intake survey, and gut microbiota were examined pre and post-intervention. The gut microbiota analysis included the relative abundance and the Shannon index. The food intake was recorded for two days for personalized nutrition education, followed by three months of personalized nutrition intervention. Statistical analysis was performed using the Wilcoxon signed-rank test in SPSS 26.0, with the significance set to p < 0.05. Results: The relative abundance of the gut microbiota changed after personalized nutrition management, with a significant decrease in the presence of Veillonella (p = 0.048). Furthermore, when the gut microbiota was analyzed according to the type of food that triggers symptoms, the diversity was increased significantly in the high fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) type (p = 0.031) and FODMAPs-containing gluten-type personalized nutrition intervention types (p < 0.001). Conclusions: Gut microbial diversity and gut microbiota distribution changed after using web-based personalized nutrition management. Hence, personalized nutrition management that considers trigger foods may improve IBS symptoms.

• Journal of The Korean Association For Science Education
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• v.23 no.2
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• pp.117-130
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• 2003

The study is to analyze the middle school-science parts among the products of nation-wide science exhibition for eleven years recently and the results of compared to the content of science-textbooks of middle school. The number of observation in the student parts among the products of nation-wide science exhibition was the highest in all methods studied, whereas, most teachers surveyed experiments in their products. The mean Shannon's diversity index (DI) was same trend for students and teachers. There was a non-significant difference of the methods in students and teacher groups according to years and the association between students and teachers were closely connected with methods. The correlation between the content of students' products and texts of middle-school was highly positive for earth science, chemistry, and biology, whereas physics showed negative correlation.

• The Korean Journal of Mycology
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• v.49 no.1
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• pp.109-118
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• 2021

In this study, we analyzed the diversity of ectomycorrhizal (ECM) fungal communities of Quercus spp. roots in the limestone area. We identified 45 generd of ECM using next generation sequencing (NGS) analysis. Soil chemical composition analysis confirmed soil pH, substitution calcium concentration, total nitrogen content, organic phosphate, and organic matter content. Shannon's Index was calculated according to the changes in soil chemical composition. The results of cluster analysis showed that Sebacina, Tomentella, Tuber, Densospora, Inocybe, Suillus, and Piloderma were the main genera of symbiotic ECM fungi that thrived in soil with high pH and calcium content.

• Journal of Environmental Science International
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• v.20 no.6
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• pp.701-710
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• 2011

The purpose of this study is to investigate the vegetation structure and ecological restoration of disturbed forest due to artificial plant. To this end, 12 plots were set up and surveyed. The result analyzed considering mean importance percentage(M.I.P) showed that the types were divided into three groups which are artificial planted forest type(three plots), natural forest-artificial planted forest type(four plots), natural forest type(five plots). Dominant proportion of artificial planted species were as follows: artificial planted forest type was over 60%, natural forest-artificial planted forest types were 14~49%. The range of Shannon's index of all associations was from 0.7131 to 0.7771(natural forest-artificial planted forest > natural forest > artificial planted forest). Also we suggested restoration method of vegetation for ecological value as follow: Control of density considering step and Remove of Pinus koraiensis seedlings of understory layer and shurb layer.

• Korean Journal of Environment and Ecology
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• v.18 no.3
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• pp.279-287
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• 2004

To investigate the vegetation structure of the section of Nakdong-jeongmaek from Gajisan to Neungdongsan, fifty plots of 100$m^2$ at ten locations were set up. In accordance with the requirements of DCA(detrended correspondance analysis), which is an ordination technique, the sites were chosen contiguously with each other. The vegetation structure analysis showed Quercus dentata, Pinus densiflora, Q. mongolica to be the major species at canopy layer, Rhododendron schlippenbachii at the layer below the canopy and Tripterygium regelii at the shrub level. Shannon's diversity index was 1.5315～2.4005 per unit area of 500$m^2$. It appeared that the Quercus dentata forest of this ridge section of Nakdong-jeongmaek is about 30 or 40 years old.

• Journal of Korean Society of Forest Science
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• v.90 no.2
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• pp.169-175
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• 2001

Genomic DNAs were extracted from the leaves of 182 ginkgo trees (Ginkgo biloba L.) planted in 6 regions and subjected to the analysis of both I-SSR and RAPD markers. A total of 227 amplicon variants were generated by PCR using 15 I-SSR primers and 67 amplicons by PCR with 5 RAPD primers. Levels of genetic diversity within 6 populations were turned out to be similar (Shannon's Index, I-SSR : 0.35~0.40; mean of 0.38, RAPD : 0.31~0.38; mean of 0.35, combined : 0.35~0.40; mean of 0.37). Ranks of the level of genetic diversity estimated from I-SSR, RAPD, and combined data were not coincided each other. Majority of genetic diversity was allocated among individuals within populations (I-SSR : 94.31%, RAPD : 93.62%, combined : 93.57%), which resulted in pretty low level of population differentiation. Genetic differentiation between male and female groups was turned out to be quite low (I-SSR : 0.03, RAPD : 0.091, combined : 0.043), which slightly fluctuated when analysis was restricted to the data obtained from 3 regions where both male and female trees were sampled (I-SSR : 0.038, RAPD : 0.084, combined : 0.047). Genetic relationships among the populations, reconstructed by UPGMA, were not coincided with geographic affinity, which might be resulted from sharing of seed sources in some regions. Whereas independent cluster analyses with I-SSR data and RAPD data, respectively, reclassified by sexes revealed two sexual groups in which all the male and the female populations were clustered together, cluster analysis with combined data did not show clear sexual grouping.

• Journal of Korean Society of Forest Science
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• v.94 no.1 s.158
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• pp.26-33
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• 2005

The study was carried out to provide information about growth and ecological characteristics by site types of natural deciduous forest in Pyeongchang, Gangwon Province. The data were collected from four aspects(the East, the West, the South, and the North) with three elevation(higher than 1.000 m, 700~1,000 m, lower than 700 m) and three topographical conditions(ridge, hill, and valley). Growth parameters by site types were evaluated based on the growth performances of dbh for the last 5 to 10 years; which were also estimated based on both Pressler and Schneider formulae. In addition, ecological characteristics such as Shannon-Wiener's diversity index, evenness index and richness index were analyzed by site types. The management methods by site types were suggested by considering stand density, growth patterns, and ecological characteristics. It was found that the stands on the South and the East are necessary the tending practices to improve growth and ecological characteristics. On the other hand, the West and the North needed the tending practices focusing on stand density management to increase growth rate rather than ecological management. It was also found that the area lower than 700 m in elevation showed Higher growth rate than other areas. As a management standard considered in terms of growth rate, it was suggested that tending practices should be applied to the stands of which growth rate for the last 5 to 10 years were lower than 2%. Considering topographical conditions, valley area showed better ecological characteristics than both ridge and hilly areas. Consequently, it was revealed that the valley areas were suitable for the ecological management.