Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Phylogenetic Analysis of Bacterial Populations in a Tomato Rhizosphere Soil Treated with Chicken Feather Protein Hydrolysate

닭우모 단백질 가수분해물을 처리한 토마토 근권토양 내 세균군집의 계통 해석

  • Kim, Se-Jong (Department of Microbial & Nano Materials, Mokwon University) ;
  • Han, Song-Ih (Department of Microbial & Nano Materials, Mokwon University) ;
  • Whang, Kyung-Sook (Department of Microbial & Nano Materials, Mokwon University)
  • 김세종 (목원대학교 미생물나노소재학과) ;
  • 한송이 (목원대학교 미생물나노소재학과) ;
  • 황경숙 (목원대학교 미생물나노소재학과)
  • Received : 2013.09.23
  • Accepted : 2013.10.15
  • Published : 2013.12.31
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Abstract

As a result of conducting a cultural experiment of tomato using chicken feather protein hydrolysate (CPH) which was mass produced by keratin protein degrading bacterium Chryseobacterium sp. FBF-7 (KACC 91463P), we found that the stem and the root of tomato showed significant improvement in growth. For the purpose of phylogenic interpretation, a comparison was drawn between the effect of CPH, a treated CPH and untreated, on the changes of bacterial populations by 454 pyrosequencing based on 16S rRNA gene sequences. Tomato rhizosphere soil untreated with CPH (NCPH) showed 6.54 Shannon index from 3,281 sequence reads, and the rhizosphere soil treated with CPH (TCPH) showed 6.33 Shannon index from 2,167 sequence reads, displaying that it does not affect the diversity. Bacterial populations were composed of 19 phyla in the rhizosphere soil, and the phylum Proteobacteria occupied 40% of total bacterial populations. Bradyrhizobium, Agromonas, Nitrobacter, and Afipia (BANA group) which belong to Bradyrhizobiaceae were abundant and commonly detected in both the treated and untreated soils, suggesting the dominance of bacterial group in rhizosphere soil. The results obtained showed that CPH treatment does not affect the indigenous bacterial populations present in the rhizosphere soil.

케라틴 단백질 분해 세균 Chryseobacterium sp. FBF-7(KACC 91463P)을 이용하여 대량생산한 닭우모 단백질 가수분해물(CPH)을 토마토에 처리한 결과, 토마토 줄기와 뿌리의 생장이 현저하게 증가되었다. 닭우모 가수분해물을 처리한 토마토 근권토양 내 세균군집 변동에 대한 계통학적 해석을 위하여 16S rRNA 유전자 서열을 기반으로 454 pyrosequencing을 수행하였다. 가수분해물을 처리하지 않은 토마토 근권토양(NCPH)의 16S rRNA 유전자 염기서열(3,281 reads)과 가수분해물을 처리한 토마토 근권토양(TCPH)의 16S rRNA 유전자 염기서열(2,167 reads)은 각각 6.33과 6.54의 다양성 지수를 나타내어 세균군집의 다양성에는 영향을 미치지 않는 것으로 확인되었다. 각 토마토 근권토양에는 총 19개의 문(phyla)의 세균이 존재하였고, 이중의 약 40%가 Proteobacteria이었다. Proteobacteria의 Bradyrhizobiaceae에 속하는 Bradyrhizobium, Agromonas, Nitrobacter 그리고 Afipia (BANA group)는 NCPH와 TCPH의 모든 근권토양에서 우점을 이루어 닭우모 가수분해믈 처리에 의해 토양 토착세균 군집에 영향을 미치지 않는 것으로 확인되었다.

Keywords

References

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