• 제목/요약/키워드: IG

검색결과 3,587건 처리시간 0.038초

콩 P34 단백질 결핍 유전자를 이용한 SSLP 마커 개발 (Development of SSLP Marker Targeted to P34 Null Gene in Soybean)

  • 양기웅;고종민;이영훈;전명기;정찬식;백인열;김현태;박금룡
    • 한국육종학회지
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    • 제42권5호
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    • pp.502-506
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    • 2010
  • 콩 알레르기에 민감한 사람들은 15가지가 넘는 콩 알레르기 단백질을 인지한다. 이러한 알레르기 단백질 때문에 콩의 광범위한 사용이 제한적이다. 시스테인 프로테아제에 속하는 P34 단백질은 콩의 주된 알러젠이다. 미국 국무부에서 16,226개의 유전자원에서 P34 단백질이 결핍된 PI567476 유전자원을 찾아냈다. P34 유전자 염기서열과 P34 유전자가 결실된 염기서열을 NCBI 데이터베이스에서 확인한 결과 P34 유전자가 결실된 염기서열에서 4 bp 가 삽입되었다는 것을 확인하였고, 그 부위에서 SSLP 마커를 개발하였다. 본 연구에서는 태광콩과 PI567476을 이용한 교배조합 $F_2$ 339개체를 개발한 분자표지마커로 확인하였다. 실험결과 태광콩 유형과 heterozygous 유형 및 PI567476 유형의 분리비는 85: 187: 67로 $X^2{_{0.05}}=5.99$, df=2에서 1:2:1의 분리비로 하나의 유전자가 관여한다는 것으로 나타났다. 앞으로 P34 단백질 관련 분자마커가 단백질 수준에서 정확히 일치하는지 확인 할 것이다.

거래당사자간 합의에 기반하는 온라인 전자금융 2-WAY 거래인증 모델 제안 (Proposal for 2-WAY Trade Verification Model that Based on Consensus between Trading Partners)

  • 이익준;오재섭;염흥열
    • 정보보호학회논문지
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    • 제28권6호
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    • pp.1475-1487
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    • 2018
  • 금융 회사 전자금융 거래 시 송금인이 수취인에게 자금을 이체할 경우 송금인은 출금 계좌 번호와 출금 금액 그리고 금융 회사에 사전 등록한 비밀번호 또는 금융 회사가 사전에 배부한 인증 매체에서 제공하는 정보 등 이용자 인증 정보를 입력하여 계좌 이체를 수행한다. 그러나, 현재 이러한 금융 회사와 송금인간 발생하는 이용자 인증 중심의 단방향 거래는 착오 송금 또는 보이스피싱 사기 거래 등 사고 위험에 노출되어 있다. 따라서, 본 연구에서는 송금인과 금융회사 이외 수취인과도 자금 이체 거래 시 거래 내용을 공유하여 수취인이 확인 후 응답하면 이체가 성립될 수 있도록 상호합의가 가능한 온라인 전자금융 2-WAY 거래인증 모델을 제안한다 기존 단방향 전자금융 거래 이체 방법을 양방향 거래 방법으로 개선하여 착오 송금, 보이스피싱 사기 예방 이외 대여금 거래, 계약 거래 등 다양한 용도로 활용하여 금융 회사 계좌이체 거래 이용자의 불편 감소 및 편리성 강화와 금융 회사의 P2P거래 활성화 등의 기대효과를 창출하고자 한다.

황칠 추출물을 첨가한 청국장이 마우스의 면역기능에 미치는 효과 (Effect of Dendropanax morbifera extract Addition on Chungkukjang on Immune Response of Mice)

  • 김진솔;정민주;정경아;송선영;이현화
    • 한국산학기술학회논문지
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    • 제20권6호
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    • pp.430-438
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    • 2019
  • 본 연구는 황칠추출물과 청국장의 기능성 강화를 위해 황칠추출물이 첨가된 청국장의 면역 활성 효능을 검증하고자 실시하였다. 황칠추출물 250, 500, 750 mg/kg 투여군, 청국장 400 mg/kg 투여군, 청국장 400 mg/kg에 황칠추출물 500 mg/kg을 첨가한 투여군으로 나누어 9주간 마우스에 경구 투여 한 후 체중 및 장기무게, 항체 생산 세포 수, 혈청 면역글로불린의 농도를 측정하고 지라의 조직을 검사하였다. 황칠추출물과 황칠추출물을 첨가한 청국장의 투여가 수컷 마우스의 체중과 장기무게에 미치는 영향을 조사한 결과 모든 투여군에서 체중과 각 장기의 무게는 유의성 있는 차이를 보이지 않았다. 양적혈구에 대한 항체 생산 세포 수 측정에서는 황칠추출물 250 mg/kg과 500 mg/kg 투여군에서 유의성 있게 증가하였으며, 림프구은 황칠추출물 500 mg/kg 투여군이 대조군보다 증가하였다. 황칠추출물 500 mg/kg을 첨가한 청국장 400 mg/kg 투여군은 대조군에 비교하여 IgG 수치가 증가하였으며, 지라 조직 검사결과 백색수질의 증가가 관찰되었다. 이러한 결과는 황칠추출물의 영향으로 황칠 추출물을 첨가한 청국장이 생체 내 면역기능 활성에 효과가 있음을 나타내며 향후 다양한 기능성식품 개발 가능성과 면역증진 식품 소재로 활용될 수 있음을 시사한다.

Selection of Vaccinia Virus-Neutralizing Antibody from a Phage-Display Human-Antibody Library

  • Shin, Yong Won;Chang, Ki-Hwan;Hong, Gwang-Won;Yeo, Sang-Gu;Jee, Youngmee;Kim, Jong-Hyun;Oh, Myoung-don;Cho, Dong-Hyung;Kim, Se-Ho
    • Journal of Microbiology and Biotechnology
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    • 제29권4호
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    • pp.651-657
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    • 2019
  • Although smallpox was eradicated in 1980, it is still considered a potential agent of biowarfare and bioterrorism. Smallpox has the potential for high mortality rates along with a major public health impact, eventually causing public panic and social disruption. Passive administration of neutralizing monoclonal antibodies (mAbs) is an effective intervention for various adverse reactions caused by vaccination and the unpredictable nature of emerging and bioterrorist-related infections. Currently, vaccinia immune globulin (VIG) is manufactured from vaccinia vaccine-boosted plasma; however, this production method is not ideal because of its limited availability, low specific activity, and risk of contamination with blood-borne infectious agents. To overcome the limitations of VIG production from human plasma, we isolated two human single-chain variable fragments (scFvs), (SC34 and SC212), bound to vaccinia virus (VACV), from a scFv phage library constructed from the B cells of VACV vaccine-boosted volunteers. The scFvs were converted to human IgG1 (VC34 and VC212). These two anti-VACV mAbs were produced in Chinese Hamster Ovary (CHO) DG44 cells. The binding affinities of VC34 and VC212 were estimated by competition ELISA to $IC_{50}$ values of $2{\mu}g/ml$ (13.33 nM) and $22{\mu}g/ml$ (146.67 nM), respectively. Only the VC212 mAb was proven to neutralize the VACV, as evidenced by the plaque reduction neutralization test (PRNT) result with a $PRNT_{50}$ of ~0.16 mg/ml (${\sim}1.07{\mu}M$). This VC212 could serve as a valuable starting material for further development of VACV-neutralizing human immunoglobulin for a prophylactic measure against post-vaccination complications and for post-exposure treatment against smallpox.

Escherichia coli-Derived Outer Membrane Vesicles Deliver Galactose-1-Phosphate Uridyltransferase and Yield Partial Protection against Actinobacillus pleuropneumoniae in Mice

  • Quan, Keji;Zhu, Zhuang;Cao, Sanjie;Zhang, Fei;Miao, Chang;Wen, Xintian;Huang, Xiaobo;Wen, Yiping;Wu, Rui;Yan, Qigui;Huang, Yong;Ma, Xiaoping;Han, Xinfeng;Zhao, Qin
    • Journal of Microbiology and Biotechnology
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    • 제28권12호
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    • pp.2095-2105
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    • 2018
  • In our previous studies, we have identified several in vivo-induced antigens and evaluated their potential as subunit vaccine candidates in a murine model, in which the recombinant protein GalT showed the most potent immunogenicity and immunoprotective efficacy against Actinobacillus pleuropneumoniae. To exploit a more efficient way of delivering GalT proteins, in this study, we employed the widely studied E. coli outer membrane vesicles (OMVs) as a platform to deliver GalT protein and performed the vaccine trial using the recombinant GalT-OMVs in the murine model. Results revealed that GalT-OMVs could elicit a highly-specific, IgG antibody titer that was comparable with the adjuvant GalT group. Significantly higher lymphocyte proliferation and cytokines secretion levels were observed in the GalT-OMVs group. 87.5% and 50% of mice were protected from a lethal dose challenge using A. pleuropneumoniae in active or passive immunization, respectively. Histopathologic and immunohistochemical analyses showed remarkably reduced pathological changes and infiltration of neutrophils in the lungs of mice immunized with GalT-OMVs after the challenge. Taken together, these findings confirm that OMVs can be used as a platform to deliver GalT protein and enhance its immunogenicity to induce both humoral and cellular immune responses in mice.

Immunomodulatory Properties of Lactobacillus plantarum NC8 Expressing an Anti-CD11c Single-Chain Fv Fragment

  • Liu, Jing;Yang, Guilian;Gao, Xing;Zhang, Zan;Liu, Yang;Yang, Xin;Shi, Chunwei;Liu, Qiong;Jiang, Yanlong;Wang, Chunfeng
    • Journal of Microbiology and Biotechnology
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    • 제29권1호
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    • pp.160-170
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    • 2019
  • The lactic acid bacteria species Lactobacillus plantarum (L. plantarum) has been used extensively for vaccine delivery. Considering to the critical role of dendritic cells in stimulating host immune response, in this study, we constructed a novel CD11c-targeting L. plantarum strain with surface-displayed variable fragments of anti-CD11c, single-chain antibody (scFv-CD11c). The newly designed L. plantarum strain, named 409-aCD11c, could adhere and invade more efficiently to bone marrow-derived DCs (BMDCs) in vitro due to the specific interaction between scFv-CD11c and CD11c located on the surface of BMDCs. After incubation with BMDCs, the 409-aCD11c strain harboring a eukaryotic vector pValac-GFP could lead to more efficient expression of GFP compared with wild-type strains shown by flow cytometry analysis, indicating the enhanced translocation of pValac-GFP from L. plantarum to BMDCs. Similar results were also observed in an in vivo study, which showed that oral administration resulted in efficient expression of GFP in both Peyer's patches (PP) and mesenteric lymph nodes (MLNs) within 7 days after the last administration. In addition, the CD11c-targeting strain significantly promoted the differentiation and maturation of DCs, the differentiation of $IL-4^+$ and $IL-17A^+$ T helper (Th) cells in MLNs, as well as production of $B220^+$ $IgA^+$ B cells in the PP. In conclusion, this study developed a novel DC-targeting L. plantarum strain which could increase the ability to deliver eukaryotic expression plasmid to host cells, indicating a promising approach for vaccine study.

Seroepidemiology of Hepatitis Viruses and Hepatitis B Genotypes of Female Marriage Immigrants in Korea

  • Kwon, Jae-Cheol;Chang, Hye Young;Kwon, Oh Young;Park, Ji Hoon;Oh, In Soo;Kim, Hyung Joon;Lee, Jun Hyung;Roh, Ha-Jung;Lee, Hyun Woong
    • Yonsei Medical Journal
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    • 제59권9호
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    • pp.1072-1078
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    • 2018
  • Purpose: The Korean society has moved rapidly toward becoming a multicultural society. This study aimed to estimate the seroprevalence of hepatitis viruses and investigate hepatitis B virus (HBV) genotypic diversity in female marriage immigrants. Materials and Methods: Screening program was conducted at support centers for multicultural families in 21 administrative districts in Korea between July 2011 and January 2017. A total of 963 female marriage immigrants were included in this study. Blood samples were tested for hepatitis viral markers and HBV genotype. Results: Subjects' median age was 33 years (20-40 years), and they originated from nine countries including Vietnam (n=422, 43.8%), China (n=311, 32.3%), the Philippines (n=85, 8.8%), Cambodia (n=58, 6.0%), and Japan (n=39, 4.0%). About 30% (n=288) of subjects required hepatitis A vaccination. HBsAg positive rate was 5.4% (n=52). Positive HBsAg results were the highest in subjects from Southeast Asia (6.6%, n=38). Anti-HBs positive rate was 60.4% (n=582). About 34% (n=329) of subjects who were negative for anti-HBs and HBsAg required HBV vaccinations. Genotypes B and C were found in 54.6% (n=12) and 45.4% (n=10) of the 22 subjects with HBV, in whom genotypes were tested. Eight (0.8%) subjects were positive for anti-HCV. Positive anti-HCV results were the highest in subjects from Central Asia (7.9%, n=3). Conclusion: Testing for hepatitis viral marker (hepatitis A virus IgG and HBsAg/anti-HBs) is needed for female marriage immigrants. Especially, HBV genotype B is different from genotype C of Koreans. Therefore, interest and attention to vaccination programs for female marriage immigrants are necessary for both clinicians and public health institutes.

Clinical features of Epstein-Barr Virus-associated Infectious Mononucleosis According to Age Group in Children

  • Lee, Soram;Chung, Ju-Young;Park, Jung Je;Seo, Ji-Hyun;Kim, Jae Young;Yeom, Jung Sook;Park, Eun-Sil;Lim, Jae-Young;Woo, Hyang-Ok;Youn, Hee-Shang
    • 고신대학교 의과대학 학술지
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    • 제33권3호
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    • pp.347-357
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    • 2018
  • Objectives: Few studies of pediatric Epstein-Barr virus (EBV)-associated infectious mononucleosis (IM) have been conducted in Korea. We evaluated the clinical features of children with IM to define differences according to age. Methods: We conducted retrospective chart reviews of 68 children aged 0 to 15 years who were diagnosed by EBV-associated IM with EBV-Viral Capsid Antigen(VCA) IgM at laboratory test and were admitted between 2010 and 2014. The children were classified into four age groups: aged 0-3, 4-6, 7-9, and 10-15 years. Results: The age distribution of patients was as follows: 19 (27.9%) 0-3, 25 (36.8%) 4-6, 13 (19.1%) 7-9, and 11 (16.2%) 10-15. Fever was the most common presentation regardless of age. It was more common in the 0-3 group than the 4-6 group (P = 0.018). Pharyngitis was more common in the 7-9 group than the 0-3 group (P = 0.048), and myalgia was more common in the 10-15 group than the 0-3 group (P = 0.007). Pharyngitis was accompanied by lymphadenopathy, protracted fever, and rash. In the 0-3 age group, the prevalence of rash was higher while the percentage of atypical lymphocytes was lower, but there was no statistical support for this tendency. There were no differences in the frequency of hepatosplenomegaly or laboratory findings between age groups. Conclusions: IM is not uncommon in young children and its clinical presentation varies with age. Therefore, IM should be suspected in young febrile children with pharyngitis and rash despite low percentages of atypical lymphocytes.

열처리 온도에 의한 구조 결정성이 탄소섬유의 전자파 차폐 성능에 미치는 영향 (The Effect of Crystallization by Heat Treatment on Electromagnetic Interference Shielding Efficiency of Carbon Fibers)

  • 김종구;정철호;이영석
    • 공업화학
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    • 제22권2호
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    • pp.138-143
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    • 2011
  • 열처리 온도에 따른 탄소섬유의 전자파 차폐특성을 알아보고자 전기방사법을 이용하여 나노섬유를 제조하고 1073, 1323, 1873, 2573 K의 온도 조건에서 열처리 공정을 실시하여 서로 다른 탄소 결정화도를 갖는 PAN계 탄소섬유를 제조하였다. 장방출 주사전자현미경을 통하여 제조된 섬유의 표면 형상을 조사하였고 열처리 온도에 따른 탄소섬유의 결정화도를 Raman 분석을 통하여 확인하였다. 또한 결정화도에 따른 전기전도성을 알아보고자 4-탐침법을 이용하여 표면저항을 측정하고 전기전도성을 계산하였으며, 회로망 분석기를 이용하여 800~4500 MHz의 주파수 영역에서 S-parameter를 측정하고 유전율 및 투자율, 그리고 전자파 차폐 특성을 조사하였다. 2573 K에서 제조된 탄소섬유의 경우 Raman 분석을 통하여 Ig/Id 값이 2.66으로 1323 K에서 제조된 탄소섬유의 1.08에 비해 2.4배 증가하여 결정화도가 향상됨을 확인하였고 전기전도성 또한 54.7 S/cm로 약 6배의 향상을 확인하였다. 유전율 실수부에서는 평균 20의 수치를 보여 1323 K에서 제조된 탄소섬유와 비교하여 약 4배의 향상을 보였다. 결과적으로 열처리 온도에 따른 탄소의 결정화도 향상에 의해 전자파 차폐 성능이 평균 41.7 dB로 약 10 dB이 향상되었음을 확인하였다.

Prevalence of Toxoplasma gondii Antibodies in Stray Dogs from Various Locations in West and East Malaysia

  • Watanabe, Malaika;Sadiq, Mohammed Babatunde;Mulop, Nazrul Iqbal Abdul;Mohammed, Konto;Rani, Puteri Azaziah Megat;Fong, Lau Seng;Aziz, Nor Azlina;Kamaludeen, Juriah;Ramanoon, Siti Zubaidah;Mansor, Rozaihan;Ping, Tan Li;Syed-Hussain, Sharifah Salmah
    • Parasites, Hosts and Diseases
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    • 제58권5호
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    • pp.487-492
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    • 2020
  • Toxoplasmosis is caused by an obligate intracellular protozoan parasite; Toxoplasma gondii, which is one of the most important zoonotic parasite worldwide. In dogs, the sexual reproductive cycle of T. gondii is lacking, and the animals are not widely consumed as food, but they are vital in the mechanical transmission of the parasite. However, there is no present data on the exposure of stray dogs to T. gondii in Malaysia. The objective of this serological survey was to determine the prevalence of T. gondii antibodies (IgG) and associated factors in stray dogs in East and West Malaysia. Antibodies to T. gondii were determined in serum samples from 222 stray dogs from 6 different states in East and West Malaysia (Peninsular Malaysia) using an Indirect ELISA. The seroprevalence for T. gondii was 23.4% (Confidence interval: CI 17.8-29.2%). Stray dogs from Selangor and Kuala Lumpur had the highest seroprevalence (32.4%; CI 13.2-45.5%) and lowest in those from Penang and Kedah (12.5%; CI 1.3-23.5%). Gender and breed were not associated with T. gondii seropositivity. However, adult dogs were more likely to be seropositive for T. gondii (OR=2.89; CI 1.1-7.7) compared with younger dogs. These results revealed that T. gondii is prevalent in stray dogs in the studied areas in Malaysia, and indicative of the level of environmental contamination of this parasite especially in urban areas.