• Proceeding of EDISON Challenge
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• 2014.03a
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• pp.287-299
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• 2014

Bicarbonate anion ($HCO_3{^-}$) takes the role of major buffer systems in our body by maintaining the pH at 7.4. Epithelial $HCO_3{^-}$ secretion also hydrolyzes the mucus which protects body from noxious infections. It has been widely known that such infections are closely related to $HCO_3{^-}$ permeability through membrane and, thus, increasing the $HCO_3{^-}$ permeability is essential. To evaluate the $HCO_3{^-}$ permeability through ion channels, the free energy changes relevant to ion pumping are calculated with the Integral Equation Formalism-PCM (IEF-PCM) theory. Molecular structures of various anions including $HCO_3{^-}$ were optimized with the density functional theory at the level of B3LYP/6-311++G(d,p) in gas and solution phase. In addition, the anion permeability is significantly influenced by the relative size of the anion and pore. We introduce a shifted volume factor model that describes the pore size effect when the charged solutes transfer through ion channels. We found excellent agreement between experimental and calculated permeability when our novel model of the size effect was taken into account to.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.2
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• pp.129-133
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• 1997

A total 943 accession of soybeans (G. max) and 50 wild soybeans (G. soja) were examined for leucine aminopeptidase (LAP) isozyme variation by 5％ polyacrylamide gel electrophoresis(PAGE) and isoelectric focusing(IEF) of pH 4~6.5. The Lap1*b by PAGE was the most common phenotype in both G. max and G. soja. The frequency of Lap1*b allele was observed to be higher in G. max(1.00) than in G. soja(0.96) of Korea. This result shows that G. max is fixed for Lapl*b allele at the Lap1 locus. LAP isozyme band type I and II were found using IEF of pH 4~6.5 in G. max and G. soja of Korea. Type I was observed from 92.8％ in G. max and 92.0％ in G. soja, and type II was discovered in 7.2％ G. max and 8.0％ G. soja. This result suggested the possibility to be found more various band types.

• Parasites, Hosts and Diseases
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• v.34 no.1
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• pp.79-86
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• 1996

A strain, KA/S2, isolated from Korean soil and morphologically assigned to Acanthcmoebc cQsteLlcnii, was characterized by isoenzyme analysis , and total proteins profile, End mitochondrial (Mt) DNA restriction fragment length polymorphism (RFLP) , and compared with four reference strains assigned to the species (the authenitic Castellani, Neff, Ma, and Chang strains). It was found that four isoenzyme, total proteins, and Mt DNA RFLP patterns by eight restriction endonucleases of the strain KA/S2 were identical with those of the Neff strain, isolated from soil of California, USA. The Chang strain was unique in its morphology and total protein patterns. Interstrain polymorphisms of isoensyme profiles and Mt DNA RFLP patterns were observed among the Castellani, Neff, Ma, and Chang strains. Mt DNA RFLP was confirmed to be highly appropriate for the strain characterization and identification of Acnnthamoeba spry.

• Journal of Life Science
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• v.13 no.4
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• pp.412-415
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• 2003

The purpose of this present study was to investigate the polymorphism of esterase locus for individual identification and parentage verification in Jeju native horse (JNH). Seventy three random JNH samples were studied by polyacrylamide gel isoelectric focusing(IEF) at pH 3.5 ∼ 6.0. We detected international recognized alleles, F, G, H, I, M, and an silent allele $I^o$. Gene frequencies of allele I showed 0.479 the highest, while allele H and M($I^o$) with relatively low frequencies were 0.027 and 0.014, respectively.

• Korean Journal of Animal Reproduction
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• v.16 no.4
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• pp.289-299
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• 1993

The polypeptide patterns of cellular and follicular components were analysed by SDS-PAGE and two dimensional(2-D)electrophoresis combined with isoelectric focusing (IEF) to establish protein profiles in each of the components in porcine follicles. Oocyte-cumulus complexes were cultured in M16+FCS+Gn at 39 in an atmosphere of 5% CO$_2$, in air for 35 h. At the end of the culture, the zona-free oocyte, ZP alone and cumulus cells were prepared and analysed either on 10% SDS-PAGE for the protein profile at the first dimensional gel or 2-D protein pattern. The amounts of each samples were determined for the visualization with Coomasie brilliant blue (CBB) or silver staining, thus giving useful information for the identification of specific proteins in the components or appropriate amount of samples for proper visualization. Oocyte showed 25 and 114 kd major protein band. Other minor components were additionally visualized with CBB on the same gel after silver staining procedure. Cumulus cells also showed specific proteins which is not present in the oocytes. The number of cumulus cell was proper to give major bands with CBB and additional minor bands with silver staining. To establish the degree of contamination from the remnant of the corona radiata to the ZP, zonae were differently prepared or analysed by SDS-PAGE.The preparation of the ZP in this study did not showed any contamination judged by the protein profile of the components. Also follicular fluid showed its specific protein profile without any significant differences among the different sizes of follicles. The established protein profile of each follicular component should be helpful for the identification and elimination of contaminated components, i. e., antigen preparation or immunological studies. The results also suggest that the preparation of each components in the study was appropriate and can be used for a further sensitive biochemical analysis in mammalian oocytes and early embryos.

• The Korean Journal of Zoology
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• v.38 no.2
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• pp.294-298
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• 1995

The genetic polymorphism of Protease inhibitor (Pl) in Korean population was investigated by using isoelectric focusing (IEF) in an ultra-narrow pH range,4.2-4.9, and immunoblottins. Three common alleles (Pl * Ml, Pl*2, Pl * M3) were observed and the frequencies for the alleles were Pl * M1=0.7843, Pl * M2=0.1613, Pl * M3=0.0323. In addition to the three common alleles, rare alleles (Pl *5, Pl * Z, Pl* H were detected at low-level frequency. Two unknovlm variants, which were not reported on previous studies in Korean population, were also found.

• Journal of Plant Biology
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• v.33 no.1
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• pp.73-80
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• 1990

Subcellular distribution of arginase activity was measured in leaves of Canavalia lineata. Both mitochondrial and cytosolic fraction were found to contain the arginase activity. It was noticible that cytosolic fraction contained a substantial amount of arginase activity. Different mobility of arginase from these two fractions was showed on DEAE-Sephacel chromatography and polyacrylamide gel electrophoresis. Also different pI value was showed 6.3 in cytosolic and 6.7, 7.1 in mitochondiral fraction on IEF gel electrophoresis. However, canavaine-dependent-activity (CDA) of arginase in these two fractions were not different. These results indicate that heterogenity of arginase occurs in leaves of C. lineata.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.2
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• pp.121-127
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• 1994

A procedure for the direct detection of (1-3)-$\beta$-glucanase isozymes in electrophoresis gels was developed. The procedure employed the commercial preparation of AZCL-pachyman as a chromogenic substrate for (1-3)-$\beta$-glucanases. The procedure detected the three basic isozymes which have been known to be expressed in germinating barley kernels. A major acidic and a minor isozymes were also detected in germinating kernels. The procedure was proved to be fast, simple and sensitive enough to be used for the analysis of the expression of (1-3)-$\beta$-glucanase isozymes in plant tissues. The detection limit of the procedure for the commercial preparation of Penicillium (1-3)-$\beta$-glucanase was estimated to be as low as 50$\mu$U. The procedure could be used for the investigation of (1-3)-$\beta$-glucanases in laboratories facilitated with ordinary equipments and research personnel.

• Journal of the Korean Ceramic Society
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• v.44 no.12
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• pp.690-695
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• 2007

Scandia stabilized zirconia (ScSZ) is adapted for electrolyte material of solid oxide fuel cell (SOFC) because of its high ionic conductivity and chemical stability. ScMnSZ1 powder having a composition of $((ZrO_2)_{0.89}(Sc_2O_3)_{0.1}(MnO_2)_{0.01})$ is synthesized by ultrasonic spray pyrolysis (USP) method. Porous ScMnSZ1 powder is obtained by using a pore forming agent. Microstructure and morphology, particle size distribution of porous powder synthesized with 3wt% pore forming agent are investigated. Sintered ScMnSZ1 sample with ground fine powder are also investigated their microstructure and electrical conductivity. The electrical conductivity of sintered ScMnSZ1 samples with ground fine powder was 0.082 S/cm, 0.127 S/cm and 0.249 S/cm at $750^{\circ}C$, $800^{\circ}C$ and $900^{\circ}C$, respectively.

• Bulletin of the Korean Chemical Society
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• v.31 no.9
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• pp.2493-2496
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• 2010

Erythropoietin (EPO) is mainly produced in kidney and stimulates erythropoiesis. The use of recombinant EPOs for doping is prohibited because of its performance enhancing effect. This study investigated whether biosimilar EPOs could be differentiated from endogenous one by iso-electro-focusing plus double blotting and SDS-PAGE for antidoping analysis. The established method was validated with positive control urine. The band patterns were reproducible and meet the criteria, which was made by world anti doping agency (WADA). Isoelectric focusing was conducted in pH range 2 to 6. Recormon (La Roche), Aropotin (Kunwha), Epokine (CJ Pharm Co.), Eporon (Dong-A), Espogen (LG Life Sciences), and Dynepo (Shire Pharmaceuticals) were detected in basic region. All biosimilars showed discriminative isoelectric profiles from endogenous EPO profiles, but they showed different band patterns with the reference one except Epokine (CJ Pharm Co.). Next, SDS-PAGE of biosimilar EPOs resulted in different molecular weight patterns which were distributed higher than endogenous EPO. Commercial immune assay kit as an immune affinity purification tool and immobilized antibody coated magnetic bead were tested for the purification and concentration of EPO from urinary matrix. The antibody-coated magnetic bead gave better purification yield. The IEF plus double blotting and SDS-PAGE with immunoaffinity purification method established can be used to discriminate biosimilar EPOs from endogenous EPO.