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1976년 1월1일 윤초실시

  • Korean Federation of Science and Technology Societies
    • The Science & Technology
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    • v.8 no.12 s.79
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    • pp.31-31
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    • 1975
  • 국립천문대는 B.I.H.(국제시보국:빠리에 있음)로부터 I.A.U(국제천문연맹)와 I.R.C.C.(국제무선통신자문위원회)의 결의에 의해서 1976년1월1일 세계표준시 0시에 기하여 윤초를 실시한다는 통보를 받았다. 따라서 우리나라 표준시도 1976년1월1일 오전9시를 기하여 조정해야 하는데 그방법은 1976년 1월1일 오전8시 59분60초 다음의 초를 8시59분 61초가 되게하고 이 시각을 1976년 1월1일 오전9시 0분0초가 되도록 조정해야한다. 이에 따라 현재 우리가 사용하는 시간보다 1초가 늦어지는 셈이 된다.

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Purufication and Characterization of Extracellular Collagenase from Vibrio mimicus (Vibrio mimicus 가 생산하는 collagenase의 정제 및 특성)

  • 김용태;김세권
    • Journal of Life Science
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    • v.6 no.4
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    • pp.241-249
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    • 1996
  • A collagenase was isolated from the culture filtrate of Vibrio mimicus (ATCC 33658). The enzyme was purified to homogeneity by ammonium sulfate precipitation and DEAE-Sephadex A-50 chromatography, which an activity recovery of 22%. The molecular weight of the purified enzyme was estimated to be 42 kDa by SDS-polyacrylamide gel electrophoresis and gel filtration, indication a monomer structure. The optimum pH and temperature od the enzyme for insoluble collagen (Type I) were around 7.75 and 28$\circ$C, respectively. Some chelating agents and serine protease inhibitor inactivated the enzyme, but L-cysteine and histidine did not affect the activity. The amino acid composition indicated that the collagenase contained high amounts of amino acid residues of glycine and alanine. The K$_{m}$ and R$_{cat}$/K$_{m}$ values for the collagenase, using insoluble collagen (type I) as substrate, were 2.86 mg/ml and 972.28 U/mg-protein, respectively.

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Existence Results for an Nonlinear Variable Exponents Anisotropic Elliptic Problems

  • Mokhtar Naceri
    • Kyungpook Mathematical Journal
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    • v.64 no.2
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    • pp.271-286
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    • 2024
  • In this paper, we prove the existence of distributional solutions in the anisotropic Sobolev space $\mathring{W}^{1,\overrightarrow{p}(\cdot)}(\Omega)$ with variable exponents and zero boundary, for a class of variable exponents anisotropic nonlinear elliptic equations having a compound nonlinearity $G(x, u)=\sum_{i=1}^{N}(\left|f\right|+\left|u\right|)^{p_i(x)-1}$ on the right-hand side, such that f is in the variable exponents anisotropic Lebesgue space $L^{\vec{p}({\cdot})}(\Omega)$, where $\vec{p}({\cdot})=(p_1({\cdot}),{\ldots},p_N({\cdot})){\in}(C(\bar{\Omega},]1,+{\infty}[))^N$.

Purification and Characterization of Two Endoxylanases from an Alkaliphilic Bacillus halodurans C-1

  • Tachaapaikoon Chakrit;Lee Yun-Sik;Rantanakhanokchai Khanok;Pinitglang Surapong;Kyu Khin Lay;Rho Min-Suk;Lee Si-Kyung
    • Journal of Microbiology and Biotechnology
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    • v.16 no.4
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    • pp.613-618
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    • 2006
  • Two endoxylanases from an alkaliphilic bacterium, Bacillus halodurans C-1, were purified 3.8- and 7.9- fold with specific activities of 9.4 and 19.8U/mg protein, respectively. The molecular masses of both purified enzymes were 23 and 47 kDa, respectively, and 23 kDa xylanase I (Xyl I) exhibited an optimum pH at 7.0, whereas 47 kDa xylanase II (Xyl II) showed a broad pH range of 5.0 to 9.0. The temperature optima of both xylanases were $60^{\circ}C\;and\;70^{\circ}C$, respectively. Both were stable in the pH range of 6.0 to 9.0 and 5.0 to 10.0, respectively, and they were stable up to $60^{\circ}C\;and\;70^{\circ}C$, respectively. The $K_m\;and\;V_{max}$ of Xyl I were 4.33mg/ml and $63.5{\mu}mol/min/mg$, respectively, whereas Xyl II had a $K_m$ value of 0.30 mg/ml and $V_{max}$ of $210{\mu}mol/min/mg$. Both xylanases hydrolyzed xylans from birchwood, oat spelt, and larchwood. However, they showed different modes of action; a series of xylooligosaccharides larger than xylotriose were released as the major products by Xyl I, whereas xylobiose and xylotriose were the main products by Xyl II. The maximum synergistic action of the two enzymes on hydrolysis of xylan was 2.16 with the ratio of Xyl I to Xyl II at 1:9.

A Study on the Influence of Substituting Cu Eine Particle for CuO on NiCuZn Ferrite (CuO 대신 Cu 미분말 치환이 NiCuZn Ferrite에 미치는 영향에 관한 연구)

  • Kim, Jae-Sik;Koh, Jae-Gui
    • Journal of the Korean Magnetics Society
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    • v.13 no.1
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    • pp.15-20
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    • 2003
  • Diffusion speed of Cu metal fine particle is fast better than CuO, so it will promote grain growth in sintering. In this paper, the influence on substituting Cu fine particle for CuO of NiCuZn ferrite with basic composition (N $i_{0.204}$C $u_{0.204}$Z $n_{0.612}$ $O_{1.02}$)F $e_{1.98}$ $O_{2.98}$ has been investigated with varying Cu/CuO ratio. The perfect spinel structure of sintered specimen at 90$0^{\circ}C$ was confirmed by the analysis of XRD patterns. The best condition was obtained when the ratio of Cu/CuO was 60%, and the permeability was 1100 and Ms was 87 emu/g in this condition. Cu has influenced on grain growth in sintering, substituting Cu fine particle for CuO could lower sintering temperature over the 3$0^{\circ}C$. After sintering, substituting Cu performed as good as CuO.s CuO.s CuO.

The Slow Strain Rate Dependence of Zircaloy-4 Cladding Tube in Iodine Atmosphere (I) (요드분위기에서 지르칼로이 피복재의 저변형율속도 의존성(I))

  • Choi, Y.;Kang, Y.H.;Ryu, W.S.;Rim, C.S.
    • Nuclear Engineering and Technology
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    • v.17 no.3
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    • pp.211-215
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    • 1985
  • The effects of temperature and strain rate on the I-SCC behaviors of Zircaloy-4 were investigated by constant load test at 30$0^{\circ}C$ and constant elongation rate test at 300, 350 and 40$0^{\circ}C$ in 3.34mg $I_2$/㎤. The results showed that I-SCC susceptibility increased as the strain rate decreased or the temperature increased. The empirical relation between the stress and the time to failure at 30$0^{\circ}C$ was given by 1/ $t_{f}$∝exp (0.3$\sigma$/$\sigma$$_{UTS}$-31.5) When the I-SCC susceptibility was expressed by the ratio of fracture energy in iodine atmosphere to that in the inert atmosphere, severe I-SCC susceptibility was found near 7.6$\times$10$^{-6}$ sec at 30$0^{\circ}C$ and the maximum point of I-SCC susceptibility tended to shift to the higher strain rate with increasing the temperature. The quasi-cleavage fracture was observed in I-SCC fracture surface. From these results, it was certain that the film repture step was involved as an important process in the I-SCC mechanism of Zircaloy-4.4.

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Primary Productivity and Assimilation Number in the Kyonggi Bay and the mid0eastern coast of Yellow Sea (서해 중동부 연안수역과 경기만에서 일차 생사력과 동화계수에 관한 연구)

  • 강연식;최중기
    • 한국해양학회지
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    • v.27 no.3
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    • pp.237-246
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    • 1992
  • In order to examine controlling factors on primary productivity and assimilation Number of phytoplankton, chlorophyll-a concentrations, light intensity, temperature, salinity and transparency were measured in the Kyonggi Bay and in the mid0eastern coast of Yellow Sea from March 1989 to October 1990. Chlorophyll-a concentration of phytoplankton ranged from 0.91 to 4.30 ug/; in the Kyonggi Bay, and from 0.78 to 4.97 ug/l in the mideastern coast of Yellow Sea. Daily averaged primary productivities and annual primary productivities of phytoplankton ranged from 37.23 to 1104.44 (averaged 361.54) mgC/m$^2$/day, 131.96hC/m$^2$/yr in the mid0eastern coast of Yellow Sea, respectively. Assimilation Number of phytoplankton ranged from 1.47 to 28.28 mgC/mg chl-a/hr in the Kyonggi Bay, and of phytoplankton in the Kyonggi Bay was higher than that of the mid0eastern coast of Yellow Sea. Light utilization efficiencies (a) in the P-I curve ranged from 0.03 to 0.93 [mgC/mg chl-a/hr]/[ue/m$^2$/sec]in the Kyonggi Bay, and from 0.01 to 0.62 [mgC/mg chl-a/hr]/[ue/m$^2$/sec] in the mid-eastern coast of Yellow Sea. Their results indicated that phytoplankton in the Kyonggi Bay utilized light more efficiently than those of the mid0eastern coast of Yellow Sea. The average values of I/SUB k/ were 48.15 ue/m$^2$/sec in the Kyonggi Bay, and 120.37 uE/m$^2$/sec in the mid-eastern coast of yellow Sea. It means the phytoplankton populations in the Kyonggi Bay seem to be adapted to lower light intensity than those of the mid-eastern coast of Yellow sea.

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Metamaterial CRLH Structure-based Balun for Common-Mode Current Indicator

  • Kahng, Sungtek;Lee, Jinil;Kim, Koon-Tae;Kim, Hyeong-Seok
    • Journal of Electrical Engineering and Technology
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    • v.9 no.1
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    • pp.301-306
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    • 2014
  • We proposed a new PCB-type 'common-mode current($I_c$) and differential-mode current($I_d$) detector' working for fast detection of $I_c$ and $I_d$ from the differential-mode signaling, with miniaturization effect and possibility of cheaper fabrication. In order to realize this device, we suggest a branch-line-coupler balun having a composite right- and left-handed(CRLH) one-layer microstrip phase-shifting line as compact as roughly ${\lambda}_g/14$. The presented balun obviously is different from the conventional bent-&-folded delay lines or slits on the ground for coupling the lines on the top and bottom dielectrics. As we connect the suggested balun output ports of the differential-mode signal lines via the through-port named U and coupled-port named L, $I_c$ and $I_d$ will appear at port ${\Delta}$ and port ${\Sigma}$ of the present device, in order. The validity of the design scheme is verified by the circuit-and numerical electromagnetic analyses, and the dispersion curve proving the metamaterial characteristics of the geometry. Besides, the examples of the $I_c$ and $I_d$ indicator are observed as the even and odd modes in differential-mode signal feeding. Also, the proposed device is shown to be very compact, compared with the conventional structure.

Micro Forming of Bulk Metallic Glass using the Deformation Behavior in the Supercooled Liquid Region (과냉각 액체 영역에서의 변형거동을 이용한 벌크 비정질 합금의 미세성형 기술 개발)

  • 홍경태;옥명렬;서진유
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2003.10a
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    • pp.93-96
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    • 2003
  • Recently, various bulk metallic glasses (BMG's) haying good mechanical and chemical properties were developed. BMG's can easily be deformed in the supercooled liquid region, via viscous flow mechanism. In our previous work, we evaluated the deformation behavior and some other basic properties of Z $r_{41.2}$ $Ti_{13.8}$C $u_{12.5}$N $i_{10}$B $e_{22.5}$ alloy. In this study, we investigated the micro forming of Z $r_{41.2}$ $Ti_{13.8}$C $u_{12.5}$N $i_{10}$B $e_{22.5}$ alloy. The process condition was chosen based on the viscosity data from TMA, and superalloy and Si wafer with micro patterns on the surface were used as forming die. The alloy showed good replication of the patterns. However, some stripe patterns, resembling scratches, appeared on the deformed alloy surface. These scratches can be reduced or eliminated by polishing before forming.ing.ore forming.ing.

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Terpinen-4-ol Induces Autophagic and Apoptotic Cell Death in Human Leukemic HL-60 Cells

  • Banjerdpongchai, Ratana;Khaw-on, Patompong
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.12
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    • pp.7537-7542
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    • 2013
  • Background: Terpinen-4-ol, a monoterpene, is found as the main component of essential oil extracts from many plants. In this study apoptotic and autophagic types of cell death induced by terpinen-4-ol and associated mechanisms were investigated in human leukemic HL-60 cells. Materials and Methods: The cytotoxicity of human leukemic U937 and HL-60 cells was determined by MTT assay. Cytochrome c release, expression of Bax, Bcl-2, Bcl-xl and cleaved Bid were determined by Western blotting. Cell morphology was examined under a transmission electron microscope. LC3-I/II, ATG5 and Beclin-1 levels were detected by immunoblotting. Results: Terpinen-4-ol exhibited cytotoxicity to human leukemic HL-60 but not U937 cells. The apoptotic response to terpinen-4-ol in HL-60 cells was due to induction of cytochrome c release from mitochondria and cleavage of Bid protein after the stimulation of caspase-8. There was a slightly decrease of Bcl-xl protein level. The characteristic cell morphology of autophagic cell death was demonstrated with multiple autophagosomes in the cytoplasm. At the molecular level, the results from Western blot analysis showed that terpinen-4-ol significantly induced accumulation of LC3-I/II, ATG5 and Beclin-1, regulatory proteins required for autophagy in mammalian cells. Conclusions: Terpinen-4-ol induced-human leukemic HL-60 cell death was via both autophagy and apoptosis.