• Title/Summary/Keyword: Hydrogen Accumulation

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A Study on the Actual Condition of Microplastic Contamination in Mackerel

  • Kwon, Woo-Taeg;Kim, Kui-Sung;Lee, Woo-Sik;Kwon, Lee-Seung;Rha, Young-Ah
    • Culinary science and hospitality research
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    • v.24 no.2
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    • pp.119-127
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    • 2018
  • The purpose of this study is to investigate the contamination level of micro plastics in Korean mackerel and to investigate the detection method of micro plastics in fish. This study analyzed the detection and composition of micro plastics using natural mackerel and wild mackerel. 30% hydrogen peroxide and surfactant were added to GF/C filter paper in order to detect micro plastics in the sea. If micro plastics are exposed to the ocean for a long time, accumulation of heavy metals in the water will make pollutant bioaccumulation more serious. Microscopic consequences The risk to human health of plastics has already been studied by many researchers and the risks to human health already caused by marine microorganisms and zooplankton are becoming clear. The conclusion of this study is that there is a difference between the length and height of the mackerel when it is cooked from the standpoint of food in terms of practical implications. The conclusion of this study is that the probability of micro-plastic-contaminated mackerel to reach and be consumed by end-consumers is low in academic implications. Residual contaminants adsorbed on micro plastics are absorbed by digestive organs and cause disruption, and further research on micro plastics is needed in the future.

Electrical Properties of Boron-Doped Amorphous Silicon Ambipolar Thin Film Transistor (보론 도우핑된 비정질 실리콘을 이용한 쌍극 박막 트랜지스터의 전기적 특성)

  • Chu, Hye-Yong;Jang, Jin
    • Journal of the Korean Institute of Telematics and Electronics
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    • v.26 no.5
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    • pp.38-45
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    • 1989
  • We have studied the electrical characteristics of the hydrogenated amorphous silicon (a-Si:H) ambiploar thin film transistors (TET'S)using 100ppm boron-doped a-Si:H as an active layer. The enhancement of drain current due to the double injection behavior has been observed in the p-channel operation of the TFT. The drain current decreases with time in streched exponential form when the gate voltage is positive. The result indicates that the dangling bonds created by electron accumulation show identical time dependence as the diffusion of hydrogen in the film. We observed the experimental evidence that the doping efficiency changes either when the gate bias is applied or when the light is illuminated on boron-doped a-Si:H.

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OsWRKY42 Represses OsMT1d and Induces Reactive Oxygen Species and Leaf Senescence in Rice

  • Han, Muho;Kim, Chi-Yeol;Lee, Junok;Lee, Sang-Kyu;Jeon, Jong-Seong
    • Molecules and Cells
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    • v.37 no.7
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    • pp.532-539
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    • 2014
  • We isolated a rice (Oryza sativa L.) WRKY gene which is highly upregulated in senescent leaves, denoted OsWRKY42. Analysis of OsWRKY42-GFP expression and its effects on transcriptional activation in maize protoplasts suggested that the OsWRKY42 protein functions as a nuclear transcriptional repressor. OsWRKY42-overexpressing (OsWR KY42OX) transgenic rice plants exhibited an early leaf senescence phenotype with accumulation of the reactive oxygen species (ROS) hydrogen peroxide and a reduced chlorophyll content. Expression analysis of ROS producing and scavenging genes revealed that the metallothionein genes clustered on chromosome 12, especially OsMT1d, were strongly repressed in OsWRKY42OX plants. An OsMT1d promoter:LUC construct was found to be repressed by OsWRKY42 overexpression in rice protoplasts. Finally, chromatin immunoprecipitation analysis demonstrated that OsWRKY42 binds to the W-box of the OsMT1d promoter. Our results thus suggest that OsWRKY42 represses OsMT1d-mediated ROS scavenging and thereby promotes leaf senescence in rice.

Effects of polyamines on hydrogen peroxide-scavenging enzymes in radish seedling plants under paraquat stress (Paraquat 스트레스를 받는 무 (Raphanus sativus L) 유식물에서 H2O2 분해 효소에 대한 폴리아민의 효과)

  • Jin, Chang-Duck
    • Journal of Plant Biotechnology
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    • v.36 no.2
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    • pp.149-156
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    • 2009
  • Application of exogenous polyamines (PAs) reduced the paraquat (PQ)-induced cotyledon injuries in radish seedling plants with 1 mM spermidine (Spd) being the most effective protectant. PQ injury symptoms in the cotyledons, e.g., large accumulation of $H_2O_2$, and losses of fresh weight, chlorophyll, and proteins, were significantly alleviated. Likewise, analysis of $H_2O_2$-scavenging enzymes such as catalase (CAT) and guaiacol peroxidase (GPX) showed that pretreatment with Spd among PAs remarkably increased total CAT activity and strongly retarded PQ-induced rapid decline in total GPX activity. In a native gel assay, one CAT isozyme (CAT1) and two GPX isozymes (GPX1 and a newly synthesized GPX isozyme) proved to be more responsible for PQ tolerance, as manifested by the strong increases in their activities by Spd pretreatment. Based on these results, we can suggest that PAs (especially 1 mM Spd) may function as antioxidant protectors by invoking CAT and GPX enzymes which control the endogenous $H_2O_2$ level in radish cotyledons exposed to PQ.

Sequential adsorption - photocatalytic oxidation process for wastewater treatment using a composite material TiO2/activated carbon

  • Andriantsiferana, Caroline;Mohamed, Elham Farouk;Delmas, Henri
    • Environmental Engineering Research
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    • v.20 no.2
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    • pp.181-189
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    • 2015
  • A composite material was tested to eliminate phenol in aqueous solution combining adsorption on activated carbon and photocatalysis with $TiO_2$ in two different ways. A first implementation involved a sequential process with a loop reactor. The aim was to reuse this material as adsorbent several times with in situ photocatalytic regeneration. This process alternated a step of adsorption in the dark and a step of photocatalytic oxidation under UV irradiation with or without $H_2O_2$. Without $H_2O_2$, the composite material was poorly regenerated due to the accumulation of phenol and intermediates in the solution and on $TiO_2$ particles. In presence of $H_2O_2$, the regeneration of the composite material was clearly enhanced. After five consecutive adsorption runs, the amount of eliminated phenol was twice the maximum adsorption capacity. The phenol degradation could be described by a pseudo first-order kinetic model where constants were much higher with $H_2O_2$ (about tenfold) due to additional ${\bullet}OH$ radicals. The second implementation was in a continuous process as with a fixed bed reactor where adsorption and photocatalysis occurred simultaneously. The results were promising as a steady state was reached indicating stabilized behavior for both adsorption and photocatalysis.

Inhibition Mechanism of Ammonia Nitrogen on the Granules in an Upflow Anaerobic Sludge Blanket Reactor (암모니아성 질소 첨가에 따른 상향류 혐기성 블랭킷 반응조내 입상슬러지의 저해 기작)

  • Lee, Chae Young;Han, Sun Kee;Shin, Hang Sik
    • Journal of Korean Society on Water Environment
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    • v.23 no.6
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    • pp.993-997
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    • 2007
  • The upflow anaerobic sludge blanket (UASB) reactor can be effective for treating simple organic compounds containing high concentration of ammonia nitrogen. The chemical oxygen demand (COD) removal efficiency was about 80% at ammonia nitrogen concentration up to 6,000 mg-N/L. This result also showed that it would be possible to treat propionate effectively at free ammonia nitrogen concentration up to 724 mg-N/L if sufficient time was allowed for adaptation. However the specific methanogenic activity (SMA) of granule was lower than that of granule in the reactor with lower ammonia nitrogen concentration. At 8,000 mg-N/L, the inhibition of high ammonia concentration was observed with evidence of increase of the volatile suspended solids (VSS) concentration in the effluent. It might be ascribed to the decrease in the content of extracellular polymer (ECP), which resulted to the sloughing off of obligated proton-reducing acetogens and heterogenotrophic methanogens from the exterior of granular sludge. This caused a great portion of the finely sludge to be easily washed out. Therefore, failure to maintain the balance between these two groups of microorganism cause accumulation of the hydrogen partial pressure in the reactor, which could have inhibited the growth of acetate utilizing methanogens.

Aluminum Stress Inhibits Root Growth and Alters Physiological and Antioxidant Enzyme Responses in Alfalfa (Medicago sativa L.) Roots (알팔파 뿌리에 있어서 알루미늄 스트레스 처리에 따른 뿌리 생장 저해와 생리 및 항산화 반응의 변화)

  • Min, Chang-Woo;Khan, Inam;Lee, Byung-Hyun
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.39 no.4
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    • pp.298-302
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    • 2019
  • Acidic soil significantly reduces crop productivity mainly due to aluminum (Al) toxicity. Alfalfa (Medicago sativa L.) roots were exposed to aluminum stress (Al3+) in calcium chloride (CaCl2) solution (pH4.5) and root growth, physiological and antioxidant enzyme responses were investigated. The root growth (length) was significantly inhibited after 48 h of aluminum stress imposition. Histochemical staining with hematoxylin indicated significant accumulation of aluminum in Al stress-treated root tissues. Histochemical assay were also performed to detect superoxide anion, hydrogen peroxide and lipid peroxidation, which were found to be more in root tissues treated with higher aluminum concentrations. The enzymatic activity of CAT, POD and GR in root tissues was slightly increased after Al stress treatment. The result suggests that Al stress alters root growth in alfalfa and induces reactive oxygen species (ROS) production, and demonstrates that antioxidant enzymes involved in detoxification of Al-mediated oxidative stress.

Cytochemical Evidence on Seasonal Variation of Peroxidase Activities in Cambial Region of Pinus densiflora, Ginkgo biloba, and Populus alba

  • Wi, Seung-Gon;Lee, Kwang-Ho;Kim, Yoon-Soo
    • Journal of the Korean Wood Science and Technology
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    • v.28 no.4
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    • pp.17-24
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    • 2000
  • The peroxidase activity was localized cytochemically to get an insight into its precise function in lignin biosynthesis. In this work, cerium chloride ($CeCl_3$) was used as a trapping agent for hydrogen peroxide ($H_2O_2$) generated from peroxidase. Seasonal variation of peroxidase activities in cambial region of Populus, Pinus, and Ginkgo was investigated at subcellular levels. Under transmission electron microscopy, electron dense deposits of cerium perhydroxide formed by reaction with $H_2O_2$ were observed in cambium and its immediate derivatives. The staining with $CeCl_3$ in cambium varied with growth seasons. The strongest $H_2O_2$ accumulation, regardless of tree species, appeared in May. Staining pattern of $CeCl_3$ in the cambium of poplar indicated that the production of peroxidase started in March before the opening of buds and reached the highest in May and then declined in August. Ginkgo and Pinus showed relatively late generation of $H_2O_2$ production when compared with Populus. Although Ginkgo and Pinus are classified into gymnosperms, however, the generation of peroxidase production and its duration was different from each other. Little staining appeared in all the tree samples collected in September before falling the leaves.

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Investigation on the Photooxidation of Pigment in Leaf-Burning II. Investigation and analysis of physiological reaction mechanism on the chlorophyll bleaching phenomenon (인삼 엽소병에서 색소의 광산화작용에 관한 연구 II. Chlorophyll bleaching의 생리적 반응기작에 관한 연구)

  • Yang, Deok-Jo;Yu, Hui-Su;Yun, Jae-Jun
    • Journal of Ginseng Research
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    • v.11 no.2
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    • pp.101-110
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    • 1987
  • This study was investigated and analyzed the physiological reaction mechanisms and the factors of the chlorophyll bleaching phenomenon on leaf burning-disease of the Ginseng (Panax ginseng C.A. Meyer). Chlorophyll bleaching phenomenon was mainly caused by the photooxidation of singlet oxygen and the autooxidation of hydrogen peroxide($H_2O_2$) accumulation resulted from inactivation of catalase and peroxidase. Chlorophyll bleaching phenomenon was remarkably accelerated by addition of saponin.

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Considerations of Acid Decomposition System for the Analysis of Heavy Metals in Packaging-grade Paper (포장용지류에서의 중금속 분석을 위한 산분해 전처리 방법의 탐색)

  • Lee, Tai-Ju;Ko, Seung-Tae;Kim, Hyoung-Jin
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.43 no.1
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    • pp.65-73
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    • 2011
  • The fibrous raw materials in packaging-grade paper production in Korea were mainly obtained from waste paper. The use of recycled paper has both positive and negative impacts in papermaking process. The primary positive impacts are the environmental protection and manufacturing cost reduction, and the negative impacts are the quality reduction in paper quality and the accumulation of heavy metals and other pollutants in wet- and dry-end process. This study was carried out to consider the optimum acid decomposition system with the highest recovery rate for the analysis of heavy metals in packaging-grade paper. The open digestion system using Kjeldahl apparatus and the closed digestion system using microwave oven for decomposing the organic materials in paper were compared. In both open and closed digestion method, the combination of nitric acid, hydrochloric acid and hydrogen peroxide showed higher recovery rate than using only nitric acid alone because the presence of Cl- ions in hydrochloric acid stabilizes ligand formation with metal ions. KOCC was observed to have the highest heavy metal content among the recycled paper samples. The heavy metal contents decomposed with the closed digestion system were relatively higher than with open digestion system.